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The map label for this gene is fadD25 [H]

Identifier: 159898380

GI number: 159898380

Start: 2159191

End: 2160930

Strand: Direct

Name: fadD25 [H]

Synonym: Haur_1856

Alternate gene names: 159898380

Gene position: 2159191-2160930 (Clockwise)

Preceding gene: 159898378

Following gene: 159898381

Centisome position: 34.02

GC content: 52.76

Gene sequence:

>1740_bases
ATGCTTGATCATGATCTGATGTTGGAGGCGGGTGGCAGTTCTGCGACGATTCAATCCAACCCTGCAAGTTTTGTTGAAAT
TATTCTTCAGCGGGCCAAAACCACTCCTGAAGCGATTGCGCTTAATTTTTATACAGCTGATGGCTATCGTACCAGCCTAT
CATATGGCTTATTGGCGCAGCGTTGCCAAGCAATTGCTGCCGTTTTGCAACGGGTGACCAAACCAGGCGATCGCGCTTTG
CTATTATACCCGCCTGGCTTGGGTTATGTGGAAGCATTTTTTGGCTGTTTATTCGCTGGCGTGATTGCCGTTCCTGCTTA
TCCGCCGCGACCCAATCGCCGCGATCAACGTTTGGAAGCAATTATTAGTAATGCTCAAGCACGGGTGATGCTGGCAGCCA
GCGAGGTCGTGGCTCAGCAACGCGCTTTATCGCAACAATATCCTGGCCTTGCTCAATTGCAATGGATCGCTAGTGATCTG
GTCAATAGCCAATTGGCCAGTATGTGGCAAGCTCCCAGCATCAATACCCATGATTTGGCCTTTTTGCAATATACCTCTGG
CTCAACTTCGCAACCACGCGGTGTAATGGTCAGCCACGAAAATCTGGTCTATAACTCGGGCTTGATTGCCCAAAGTTTTG
GCATCACGGCTGATGATCATGTGTTTATTTGGTTGCCGCCTTACCACGATATGGGCTTGATCGGTGGGATTATGCAGCCG
TTGTTTACTGGCTGTGAATTAAGTTTGATCGATCCCCTAACCTTTTTGCAACAACCGCTGATTTGGCTCCGCATGATCAG
CGATTTAGGGGTGACGGTCACTGGCGGGCCAAATTTTGCCTACGATTTATGCGTTGCCAAAGCCAAGCCCGAAGCCTTGG
CAGGCGTTGATCTAAGCCGTTTGCGGGTGGCATTTAATGGCGCTGAGCCAATTCGTGCAGCAACCCTTGAGCGATTTAGC
CGCACTTTTGCCCCGTTAGGCTTCAAACCTCAGGCCTTCTTGCCGTGTTATGGCTTAGCCGAAGCCACGCTCTTTGTCAG
TGGTGCGCCGCATGCTGCCGAACCAACCACACTCACGGTCGATGCTCAAGCCTTGAGCCAACATCAAGCGCTGCCAAGTG
AGCGTGGGACATTGTTGGTAAGTTCGGGCATGGTGGCCGCGCCGCAAATTGTGGCGATTGTCGATCCTGAGCAGGGCCAG
GTTTGTGCCGATGGTTGGGTTGGCGAGGTCTGCATTCACGGGCGTAGCATTGCCCATGGCTATTGGGATAACTCCGCCGC
TAGCGAGGCTACTTTTCAATTGATCTTGCCTGATGGCAGCGGCCCCTTCCTGCGCACGGGCGACCTTGGTTTTATCCACG
AAGGGCAGTTGTATATTACTGGGCGGCTCAAAGACCTGATTATTATTGATGGGCGCAATCATTACCCCCAAGACTTGGAA
TTGAGCGTTGAATTGGCGCATCCGGCAATCCGCCAAGGTGGTTGTGCCGCTTTTGCCGTCGATGGCGCTGATGGTGAGCA
AATTGTGATTGTGGCCGAAATTCGCCGCCCCAATCAAGCCGAAGAAGCGGCTCAGGCTGTGCGCTTGGCCCTCCAGCAAC
AATACGATTTAGCAATTGCCGATTTGATGTGGGTACGGCCTGGTCAAGTGCCCAAAACCTCAAGCGGCAAGGTGCGCCGC
CGCGAATGCCGCCAACGCTATTTGAGCCAAACCCTCAATAGCTTGGAGGGCGAGGAATGA

Upstream 100 bases:

>100_bases
AAAACGATCACTTTAAGCGCCTATCAATATCAGGTTAATAGCTGTTGTTGCCATGCTGGGTGTGGTGTAATGCCTTGCAG
TTGAACAGTGTGAGGTTGTT

Downstream 100 bases:

>100_bases
GTTCCAACTATAGCGCTGCGCAAATTGCAGGTTGGCTGCAAACGCAGATTGCTGAACGTTTGAAACGCTCGCCCGAACTG
GTAGCACTGACGGCTCCGTT

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MLDHDLMLEAGGSSATIQSNPASFVEIILQRAKTTPEAIALNFYTADGYRTSLSYGLLAQRCQAIAAVLQRVTKPGDRAL
LLYPPGLGYVEAFFGCLFAGVIAVPAYPPRPNRRDQRLEAIISNAQARVMLAASEVVAQQRALSQQYPGLAQLQWIASDL
VNSQLASMWQAPSINTHDLAFLQYTSGSTSQPRGVMVSHENLVYNSGLIAQSFGITADDHVFIWLPPYHDMGLIGGIMQP
LFTGCELSLIDPLTFLQQPLIWLRMISDLGVTVTGGPNFAYDLCVAKAKPEALAGVDLSRLRVAFNGAEPIRAATLERFS
RTFAPLGFKPQAFLPCYGLAEATLFVSGAPHAAEPTTLTVDAQALSQHQALPSERGTLLVSSGMVAAPQIVAIVDPEQGQ
VCADGWVGEVCIHGRSIAHGYWDNSAASEATFQLILPDGSGPFLRTGDLGFIHEGQLYITGRLKDLIIIDGRNHYPQDLE
LSVELAHPAIRQGGCAAFAVDGADGEQIVIVAEIRRPNQAEEAAQAVRLALQQQYDLAIADLMWVRPGQVPKTSSGKVRR
RECRQRYLSQTLNSLEGEE

Sequences:

>Translated_579_residues
MLDHDLMLEAGGSSATIQSNPASFVEIILQRAKTTPEAIALNFYTADGYRTSLSYGLLAQRCQAIAAVLQRVTKPGDRAL
LLYPPGLGYVEAFFGCLFAGVIAVPAYPPRPNRRDQRLEAIISNAQARVMLAASEVVAQQRALSQQYPGLAQLQWIASDL
VNSQLASMWQAPSINTHDLAFLQYTSGSTSQPRGVMVSHENLVYNSGLIAQSFGITADDHVFIWLPPYHDMGLIGGIMQP
LFTGCELSLIDPLTFLQQPLIWLRMISDLGVTVTGGPNFAYDLCVAKAKPEALAGVDLSRLRVAFNGAEPIRAATLERFS
RTFAPLGFKPQAFLPCYGLAEATLFVSGAPHAAEPTTLTVDAQALSQHQALPSERGTLLVSSGMVAAPQIVAIVDPEQGQ
VCADGWVGEVCIHGRSIAHGYWDNSAASEATFQLILPDGSGPFLRTGDLGFIHEGQLYITGRLKDLIIIDGRNHYPQDLE
LSVELAHPAIRQGGCAAFAVDGADGEQIVIVAEIRRPNQAEEAAQAVRLALQQQYDLAIADLMWVRPGQVPKTSSGKVRR
RECRQRYLSQTLNSLEGEE
>Mature_579_residues
MLDHDLMLEAGGSSATIQSNPASFVEIILQRAKTTPEAIALNFYTADGYRTSLSYGLLAQRCQAIAAVLQRVTKPGDRAL
LLYPPGLGYVEAFFGCLFAGVIAVPAYPPRPNRRDQRLEAIISNAQARVMLAASEVVAQQRALSQQYPGLAQLQWIASDL
VNSQLASMWQAPSINTHDLAFLQYTSGSTSQPRGVMVSHENLVYNSGLIAQSFGITADDHVFIWLPPYHDMGLIGGIMQP
LFTGCELSLIDPLTFLQQPLIWLRMISDLGVTVTGGPNFAYDLCVAKAKPEALAGVDLSRLRVAFNGAEPIRAATLERFS
RTFAPLGFKPQAFLPCYGLAEATLFVSGAPHAAEPTTLTVDAQALSQHQALPSERGTLLVSSGMVAAPQIVAIVDPEQGQ
VCADGWVGEVCIHGRSIAHGYWDNSAASEATFQLILPDGSGPFLRTGDLGFIHEGQLYITGRLKDLIIIDGRNHYPQDLE
LSVELAHPAIRQGGCAAFAVDGADGEQIVIVAEIRRPNQAEEAAQAVRLALQQQYDLAIADLMWVRPGQVPKTSSGKVRR
RECRQRYLSQTLNSLEGEE

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=588, Percent_Identity=26.530612244898, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI55749758, Length=514, Percent_Identity=25.875486381323, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI45827692, Length=593, Percent_Identity=25.8010118043845, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI225735629, Length=593, Percent_Identity=25.8010118043845, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI225735625, Length=607, Percent_Identity=23.8879736408567, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI45827694, Length=551, Percent_Identity=23.7749546279492, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI225735627, Length=505, Percent_Identity=23.1683168316832, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI45827696, Length=506, Percent_Identity=23.1225296442688, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI45827698, Length=478, Percent_Identity=22.8033472803347, Blast_Score=83, Evalue=9e-16,
Organism=Escherichia coli, GI1788107, Length=551, Percent_Identity=26.1343012704174, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI145693145, Length=571, Percent_Identity=25.0437828371278, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1789201, Length=348, Percent_Identity=26.1494252873563, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786801, Length=535, Percent_Identity=24.6728971962617, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193202679, Length=605, Percent_Identity=25.1239669421488, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17559526, Length=559, Percent_Identity=23.613595706619, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=586, Percent_Identity=22.6962457337884, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=582, Percent_Identity=21.4776632302405, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=528, Percent_Identity=21.969696969697, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24654656, Length=477, Percent_Identity=23.2704402515723, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI18859661, Length=401, Percent_Identity=26.6832917705736, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24581924, Length=414, Percent_Identity=24.6376811594203, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62688; Mature: 62688

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDHDLMLEAGGSSATIQSNPASFVEIILQRAKTTPEAIALNFYTADGYRTSLSYGLLAQ
CCCCCEEEECCCCCCEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHH
RCQAIAAVLQRVTKPGDRALLLYPPGLGYVEAFFGCLFAGVIAVPAYPPRPNRRDQRLEA
HHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
IISNAQARVMLAASEVVAQQRALSQQYPGLAQLQWIASDLVNSQLASMWQAPSINTHDLA
HHCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
FLQYTSGSTSQPRGVMVSHENLVYNSGLIAQSFGITADDHVFIWLPPYHDMGLIGGIMQP
EEEECCCCCCCCCCEEEECCCEEEECCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHH
LFTGCELSLIDPLTFLQQPLIWLRMISDLGVTVTGGPNFAYDLCVAKAKPEALAGVDLSR
HHCCCCEEEECHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEECCCCCHHCCCCHHH
LRVAFNGAEPIRAATLERFSRTFAPLGFKPQAFLPCYGLAEATLFVSGAPHAAEPTTLTV
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEE
DAQALSQHQALPSERGTLLVSSGMVAAPQIVAIVDPEQGQVCADGWVGEVCIHGRSIAHG
EHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEECCCCEEEEEECCCEEECC
YWDNSAASEATFQLILPDGSGPFLRTGDLGFIHEGQLYITGRLKDLIIIDGRNHYPQDLE
CCCCCCCCCEEEEEEEECCCCCEEEECCCCEEECCCEEEEECCEEEEEECCCCCCCCCCE
LSVELAHPAIRQGGCAAFAVDGADGEQIVIVAEIRRPNQAEEAAQAVRLALQQQYDLAIA
EEEEECCCHHHCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEH
DLMWVRPGQVPKTSSGKVRRRECRQRYLSQTLNSLEGEE
HHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLDHDLMLEAGGSSATIQSNPASFVEIILQRAKTTPEAIALNFYTADGYRTSLSYGLLAQ
CCCCCEEEECCCCCCEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHH
RCQAIAAVLQRVTKPGDRALLLYPPGLGYVEAFFGCLFAGVIAVPAYPPRPNRRDQRLEA
HHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
IISNAQARVMLAASEVVAQQRALSQQYPGLAQLQWIASDLVNSQLASMWQAPSINTHDLA
HHCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
FLQYTSGSTSQPRGVMVSHENLVYNSGLIAQSFGITADDHVFIWLPPYHDMGLIGGIMQP
EEEECCCCCCCCCCEEEECCCEEEECCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHH
LFTGCELSLIDPLTFLQQPLIWLRMISDLGVTVTGGPNFAYDLCVAKAKPEALAGVDLSR
HHCCCCEEEECHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEECCCCCHHCCCCHHH
LRVAFNGAEPIRAATLERFSRTFAPLGFKPQAFLPCYGLAEATLFVSGAPHAAEPTTLTV
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEE
DAQALSQHQALPSERGTLLVSSGMVAAPQIVAIVDPEQGQVCADGWVGEVCIHGRSIAHG
EHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEECCCCEEEEEECCCEEECC
YWDNSAASEATFQLILPDGSGPFLRTGDLGFIHEGQLYITGRLKDLIIIDGRNHYPQDLE
CCCCCCCCCEEEEEEEECCCCCEEEECCCCEEECCCEEEEECCEEEEEECCCCCCCCCCE
LSVELAHPAIRQGGCAAFAVDGADGEQIVIVAEIRRPNQAEEAAQAVRLALQQQYDLAIA
EEEEECCCHHHCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEH
DLMWVRPGQVPKTSSGKVRRRECRQRYLSQTLNSLEGEE
HHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]