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The map label for this gene is dhbF [H]

Identifier: 159898097

GI number: 159898097

Start: 1824589

End: 1826274

Strand: Direct

Name: dhbF [H]

Synonym: Haur_1573

Alternate gene names: 159898097

Gene position: 1824589-1826274 (Clockwise)

Preceding gene: 159898096

Following gene: 159898098

Centisome position: 28.75

GC content: 50.89

Gene sequence:

>1686_bases
ATGCCGATTTACAATAGCCAACTCTTAATTCTGAACGATGGTCTACAGCCAGTTCCCGATGGATGTATTGGCCAAATTTG
GATTAGTGGGGCTGGAGTTGGCAATGGCTATACTGGCCAGCCAGGGTTGACGGCTGAGCGTTTTCGGCCTAACCCCTTTG
ATTATATCCCTGGCTCGCGGATGTATTGCAGCGGTGATCTGGGTCGGCGTAACCAGAATGGGGCAATCGAATTTCTCGGG
CGTACTGATCATCAGGTAAAAATTCGCGGCTTTCGGGTTGAATTAACCGAAATTGCACAGCGCTTACAGCAGCATCCGGC
GGTTCGCGAGGCGCTTGTTATGCGGCACGAGCATCCGCTGCGGGGCGAATATCTTGTTGCATATGTTGTGCTGATCCAGC
CGGCTCAAGCCGATGTTGGTGCGTTGCGGCGCTATCTTGCAAAGCATAGTCCACCCTATCTCGTTCCAGCTGAAATTGTG
CTACTGGAGGCATTTCCGCTTAGCCCAAATGGCAAGATCGATCGGCAGGCGTTGCCGCATCCCACGGCGAATGTTCATGC
TGGTGAGGCTCGCCAACCCGCTGCTCGCTCATTTGACCCACTTGAGTTTCAAATACGCACAATCTGGGAAGAGACGCTTG
GCATTGAACCGATTGGGGCACAAGCGAATTTTTTTGAGCTTGGTGGGCATTCGTTGCTTGCGATTGCATTGATGGCACGG
ATCGAAGAACGGCTTGGTAAACGCTTGCCAATTACCATCCTCTTTGATGCCCCCACGATCGAGCAAATGGTCGGCTTGCT
TCGGCAGGAGGGCTATGCGCCGCGATGGTCGCCGATGGTTTTGATGCAAGCAGGGCGTAAGGGGTTTCCTGCACTATTTT
GTGTCCATGCACTTAGTGGCAGCGCCTTTGCCTACACAGTATTGCCCAAACATATCTCACCCGACCAACCATTTTATGGG
ATTGAATCGCGCGGTCGCGATATTAACCAAGCCCCTGATCGCTCGATTGAGGCCATGGCGGCCTATTATATTGAGCATAT
GCGGCTGATTCAACCTAGTGGACCATATTGTATTGCTGGATGGTCATTAGGTGGGCCAGTTGCCTTTGAAATGGCTCAGC
AATTATATCGTGCTGGTGAAACAGTGGCGTTGCTGGCGATTGTTGATACGGGAGCGCCCTTGGCTGGGCGATTACCAATT
GAACCAGCTGCCATTGATGATCTTTCTTTATTGGTGCCACGCTTACGCCATTTTGGAATTAATCTCGATTTGGACTTCAT
TCAAGCACTTCCGGCAGATCAGCAGCTTCCTTATATTATGCAACAAGCAGTGAGTGGTGGTTTTTGGTCGGCAAACATTA
CGCTTGCCGAAATTAAGCGTAAAGGTGAGCTGATTCGCACAAATTTAGCCGCACGACGCAACTATAGCGCCCAGCCCTAC
CCTGGGACAATCAACCTCTTCCGCACCAAACAACATCCAGGTTATAGCGACGACGAAGTTACGCTTGGTTGGGATCAATT
GGCTTTAACGGGAGTTAAGGTGTGGGAAATCCCTGGTGATCACCTCTCAATTTTTCATAGTCCTTATGTTGAGGTGTTGG
GGCACGCATTATGGGAGTGTCTTCAGGGTTGTACGGATCAGCTAGACGAACTCGATTACGCGCATGAACGATATCGAGCG
CAATGA

Upstream 100 bases:

>100_bases
CATTATTAATACCTATGGGCCAACTGAGTGTACTGATATTGCAGCGAGTTATTAGATTGATGCGCTTGCACAACTGGCTG
AAAAGCCAATACCAATTGGC

Downstream 100 bases:

>100_bases
ATGCAAATGAATGCATGGGAGGTTGTGAGATGCGTATTCAAGGAGCGACCCAGAGCAATCCAACCGATACAAGCCCGGAT
GACGATCAACCCTGGTGCAT

Product: thioesterase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MPIYNSQLLILNDGLQPVPDGCIGQIWISGAGVGNGYTGQPGLTAERFRPNPFDYIPGSRMYCSGDLGRRNQNGAIEFLG
RTDHQVKIRGFRVELTEIAQRLQQHPAVREALVMRHEHPLRGEYLVAYVVLIQPAQADVGALRRYLAKHSPPYLVPAEIV
LLEAFPLSPNGKIDRQALPHPTANVHAGEARQPAARSFDPLEFQIRTIWEETLGIEPIGAQANFFELGGHSLLAIALMAR
IEERLGKRLPITILFDAPTIEQMVGLLRQEGYAPRWSPMVLMQAGRKGFPALFCVHALSGSAFAYTVLPKHISPDQPFYG
IESRGRDINQAPDRSIEAMAAYYIEHMRLIQPSGPYCIAGWSLGGPVAFEMAQQLYRAGETVALLAIVDTGAPLAGRLPI
EPAAIDDLSLLVPRLRHFGINLDLDFIQALPADQQLPYIMQQAVSGGFWSANITLAEIKRKGELIRTNLAARRNYSAQPY
PGTINLFRTKQHPGYSDDEVTLGWDQLALTGVKVWEIPGDHLSIFHSPYVEVLGHALWECLQGCTDQLDELDYAHERYRA
Q

Sequences:

>Translated_561_residues
MPIYNSQLLILNDGLQPVPDGCIGQIWISGAGVGNGYTGQPGLTAERFRPNPFDYIPGSRMYCSGDLGRRNQNGAIEFLG
RTDHQVKIRGFRVELTEIAQRLQQHPAVREALVMRHEHPLRGEYLVAYVVLIQPAQADVGALRRYLAKHSPPYLVPAEIV
LLEAFPLSPNGKIDRQALPHPTANVHAGEARQPAARSFDPLEFQIRTIWEETLGIEPIGAQANFFELGGHSLLAIALMAR
IEERLGKRLPITILFDAPTIEQMVGLLRQEGYAPRWSPMVLMQAGRKGFPALFCVHALSGSAFAYTVLPKHISPDQPFYG
IESRGRDINQAPDRSIEAMAAYYIEHMRLIQPSGPYCIAGWSLGGPVAFEMAQQLYRAGETVALLAIVDTGAPLAGRLPI
EPAAIDDLSLLVPRLRHFGINLDLDFIQALPADQQLPYIMQQAVSGGFWSANITLAEIKRKGELIRTNLAARRNYSAQPY
PGTINLFRTKQHPGYSDDEVTLGWDQLALTGVKVWEIPGDHLSIFHSPYVEVLGHALWECLQGCTDQLDELDYAHERYRA
Q
>Mature_560_residues
PIYNSQLLILNDGLQPVPDGCIGQIWISGAGVGNGYTGQPGLTAERFRPNPFDYIPGSRMYCSGDLGRRNQNGAIEFLGR
TDHQVKIRGFRVELTEIAQRLQQHPAVREALVMRHEHPLRGEYLVAYVVLIQPAQADVGALRRYLAKHSPPYLVPAEIVL
LEAFPLSPNGKIDRQALPHPTANVHAGEARQPAARSFDPLEFQIRTIWEETLGIEPIGAQANFFELGGHSLLAIALMARI
EERLGKRLPITILFDAPTIEQMVGLLRQEGYAPRWSPMVLMQAGRKGFPALFCVHALSGSAFAYTVLPKHISPDQPFYGI
ESRGRDINQAPDRSIEAMAAYYIEHMRLIQPSGPYCIAGWSLGGPVAFEMAQQLYRAGETVALLAIVDTGAPLAGRLPIE
PAAIDDLSLLVPRLRHFGINLDLDFIQALPADQQLPYIMQQAVSGGFWSANITLAEIKRKGELIRTNLAARRNYSAQPYP
GTINLFRTKQHPGYSDDEVTLGWDQLALTGVKVWEIPGDHLSIFHSPYVEVLGHALWECLQGCTDQLDELDYAHERYRAQ

Specific function: Specifically adenylates threonine and glycine, and loads them onto their corresponding peptidyl carrier domains [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Homo sapiens, GI58082049, Length=192, Percent_Identity=30.2083333333333, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI115511026, Length=147, Percent_Identity=31.9727891156463, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI157311624, Length=192, Percent_Identity=29.1666666666667, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI157311622, Length=192, Percent_Identity=29.1666666666667, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI38505220, Length=160, Percent_Identity=31.25, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI42544132, Length=152, Percent_Identity=26.9736842105263, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI28416953, Length=160, Percent_Identity=30.625, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1786801, Length=399, Percent_Identity=36.0902255639098, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1786810, Length=164, Percent_Identity=31.0975609756098, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI145693145, Length=173, Percent_Identity=29.4797687861272, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790505, Length=136, Percent_Identity=30.8823529411765, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556356, Length=279, Percent_Identity=27.9569892473118, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17550940, Length=387, Percent_Identity=23.2558139534884, Blast_Score=83, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319591, Length=314, Percent_Identity=31.5286624203822, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6319699, Length=186, Percent_Identity=23.6559139784946, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648676, Length=278, Percent_Identity=30.5755395683453, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24667959, Length=136, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI62472339, Length=142, Percent_Identity=28.8732394366197, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24667955, Length=136, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 62293; Mature: 62162

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIYNSQLLILNDGLQPVPDGCIGQIWISGAGVGNGYTGQPGLTAERFRPNPFDYIPGSR
CCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
MYCSGDLGRRNQNGAIEFLGRTDHQVKIRGFRVELTEIAQRLQQHPAVREALVMRHEHPL
EEECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEHHHHHHHHHHCHHHHHHHHHHCCCCC
RGEYLVAYVVLIQPAQADVGALRRYLAKHSPPYLVPAEIVLLEAFPLSPNGKIDRQALPH
CCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
PTANVHAGEARQPAARSFDPLEFQIRTIWEETLGIEPIGAQANFFELGGHSLLAIALMAR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHH
IEERLGKRLPITILFDAPTIEQMVGLLRQEGYAPRWSPMVLMQAGRKGFPALFCVHALSG
HHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCC
SAFAYTVLPKHISPDQPFYGIESRGRDINQAPDRSIEAMAAYYIEHMRLIQPSGPYCIAG
CEEEEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCEEEEE
WSLGGPVAFEMAQQLYRAGETVALLAIVDTGAPLAGRLPIEPAAIDDLSLLVPRLRHFGI
CCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
NLDLDFIQALPADQQLPYIMQQAVSGGFWSANITLAEIKRKGELIRTNLAARRNYSAQPY
CCCHHHHHHCCCCCCCHHHHHHHHCCCEEECCEEHHHHHHCCHHHHHHHHHHCCCCCCCC
PGTINLFRTKQHPGYSDDEVTLGWDQLALTGVKVWEIPGDHLSIFHSPYVEVLGHALWEC
CCEEEEEEECCCCCCCCCCEEECHHHHEEECEEEEECCCCCEEECCCHHHHHHHHHHHHH
LQGCTDQLDELDYAHERYRAQ
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PIYNSQLLILNDGLQPVPDGCIGQIWISGAGVGNGYTGQPGLTAERFRPNPFDYIPGSR
CCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
MYCSGDLGRRNQNGAIEFLGRTDHQVKIRGFRVELTEIAQRLQQHPAVREALVMRHEHPL
EEECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEHHHHHHHHHHCHHHHHHHHHHCCCCC
RGEYLVAYVVLIQPAQADVGALRRYLAKHSPPYLVPAEIVLLEAFPLSPNGKIDRQALPH
CCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
PTANVHAGEARQPAARSFDPLEFQIRTIWEETLGIEPIGAQANFFELGGHSLLAIALMAR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHH
IEERLGKRLPITILFDAPTIEQMVGLLRQEGYAPRWSPMVLMQAGRKGFPALFCVHALSG
HHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCC
SAFAYTVLPKHISPDQPFYGIESRGRDINQAPDRSIEAMAAYYIEHMRLIQPSGPYCIAG
CEEEEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCEEEEE
WSLGGPVAFEMAQQLYRAGETVALLAIVDTGAPLAGRLPIEPAAIDDLSLLVPRLRHFGI
CCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
NLDLDFIQALPADQQLPYIMQQAVSGGFWSANITLAEIKRKGELIRTNLAARRNYSAQPY
CCCHHHHHHCCCCCCCHHHHHHHHCCCEEECCEEHHHHHHCCHHHHHHHHHHCCCCCCCC
PGTINLFRTKQHPGYSDDEVTLGWDQLALTGVKVWEIPGDHLSIFHSPYVEVLGHALWEC
CCEEEEEEECCCCCCCCCCEEECHHHHEEECEEEEECCCCCEEECCCHHHHHHHHHHHHH
LQGCTDQLDELDYAHERYRAQ
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11112781; 9384377; 10568751; 8550523 [H]