Click here to switch to the map view.

The map label for this gene is lgrC [H]

Identifier: 159898096

GI number: 159898096

Start: 1822987

End: 1824543

Strand: Direct

Name: lgrC [H]

Synonym: Haur_1572

Alternate gene names: 159898096

Gene position: 1822987-1824543 (Clockwise)

Preceding gene: 159898095

Following gene: 159898097

Centisome position: 28.72

GC content: 48.04

Gene sequence:

>1557_bases
ATGATCTTAGCGACAACGATTAAGCTGCTGCTATATCGGTATAGTAATCAAACCGATATGCTGATTGGTACGCCCGTAGC
GGGGCGTTTAACCACAGCTTTAGAATCGATTGTAGGATTTTTGGCTAATACCCTTGTATTAAGAACCTCGCTTGCTGGCC
AACCTTCTTTTCGTGTACTTCTGCAACGGGTTCGCGAGGTTGCACTTGCGGCATATGCGCACCAAGCGCTGCCCTTCGAG
CGCTTACTTCAGCAGCTCGGCCCGAATCGTAGCCAAAGCCATTTACCAGTGTTTCAAGTACTGGTTACCATCCAAAATAG
CCCTGAGGCCACGCTCCAACTGGTTGATACCAAGATTGTGCCGATTGAATTTGAGCGTGGAACGGCTAAATTTGATTTAA
GCTTTGTGTTTACTGAGACCATGAATGGCTTGGCGGGAGGCATTGAATACAACACTGATCTGTTTGATGCTGCAACGATT
GAGCGCATGATCGGGCATTTAAACAGCTTATTGCTTGCTGGTTTAAGTGCGCCAGATCTGTCAATTGCGCGTTTGCCGCT
GTTGACCACTGCTGAGTATGCGCGATATATTGCACAATCTCGTGGCATCGCGAGCAACTTCCCAAGCGAAAGCTGTGTAC
TGACCTTATTTGAACAGCAGGTGGCGCGAACACCGCATGCGCTAGCTGTTGTTCAGGGTCACGCCAGCCTTACCTATCAA
GCTTTAGATCATCAAGCGAATCAGCTAGCCTATTATCTTGCGGCCCTCGGCGTGAAACCAGGGACAGGGGTGGGTTTGTG
CATTGAGCGAACTCTAATGATGGTGGTTGGGATGATGGGTATTCTGAGGCTTGGGGCAATCTATGTGCCGATTGATCCGA
GCTATCCACCTGAACGTCAAGCTTATATGGTCGCGAATGCTGGTTTGGTTGCGCTTGTAACACAAGATCAATTGGCAAGT
GCGCTGCAAGCCCAACAGCCTAAGCTCGCAATTGTTGCGCTTGATACCGATTGGACGGCAATTGCCTGTGCCCCACGCGT
CAAACTGCCGCTCATCACGGCTGATATGGTTCTGTACAGTATCTATACATCGGGATCGACTGGAGTTCCGAAGGGCACAG
AAGTAACCCATCGTAACTTTGTGAATCTGCTCAATTGGTATATTACGGAATTTAATTTAGGGTCTGACGATCGGTTTTTG
TTAGTGACATCGTTGAGTTTTGATCTGACCCAAAAAAATGTTTTTGCGCCGTTGATCAGCGGGGGAACTCTCTATTTGTA
CGATGCGCCCCAGTATGATCCGCATACGATAACATCACTGATTGATCAGCATGCAATAACCGTCATTAATTGTACGCCAA
GCGCTTTTTATCCGCTGGTTGAAACAACGCCTGATCAGGCGATCGTGCAGCTAAAATCGCTCAGATGGCTCTTTTTAGGC
GGTGAACCGATCAATCTGAGTCGATTCCATGGGTGGATGCAATCGAGCCATTATCAGGCAACCATTATTAATACCTATGG
GCCAACTGAGTGTACTGATATTGCAGCGAGTTATTAG

Upstream 100 bases:

>100_bases
ACAGCAAACGTTTCAAGGCAAAACTGCATATTTTTCAATTAATGAGGAGCTAACCAAAGCCCTCAAAGCATTTGCTCATC
AGGAGCAAGCTAGCCTTTTT

Downstream 100 bases:

>100_bases
ATTGATGCGCTTGCACAACTGGCTGAAAAGCCAATACCAATTGGCATGCCGATTTACAATAGCCAACTCTTAATTCTGAA
CGATGGTCTACAGCCAGTTC

Product: AMP-dependent synthetase and ligase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent D-valine adenylase; D-ValA; D-valine activase; Valine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan/phenylalanine/tyrosine adenylase; Trp/Phe/TyrA; Tryptophan/phenylalanine/tyrosine activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing] [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MILATTIKLLLYRYSNQTDMLIGTPVAGRLTTALESIVGFLANTLVLRTSLAGQPSFRVLLQRVREVALAAYAHQALPFE
RLLQQLGPNRSQSHLPVFQVLVTIQNSPEATLQLVDTKIVPIEFERGTAKFDLSFVFTETMNGLAGGIEYNTDLFDAATI
ERMIGHLNSLLLAGLSAPDLSIARLPLLTTAEYARYIAQSRGIASNFPSESCVLTLFEQQVARTPHALAVVQGHASLTYQ
ALDHQANQLAYYLAALGVKPGTGVGLCIERTLMMVVGMMGILRLGAIYVPIDPSYPPERQAYMVANAGLVALVTQDQLAS
ALQAQQPKLAIVALDTDWTAIACAPRVKLPLITADMVLYSIYTSGSTGVPKGTEVTHRNFVNLLNWYITEFNLGSDDRFL
LVTSLSFDLTQKNVFAPLISGGTLYLYDAPQYDPHTITSLIDQHAITVINCTPSAFYPLVETTPDQAIVQLKSLRWLFLG
GEPINLSRFHGWMQSSHYQATIINTYGPTECTDIAASY

Sequences:

>Translated_518_residues
MILATTIKLLLYRYSNQTDMLIGTPVAGRLTTALESIVGFLANTLVLRTSLAGQPSFRVLLQRVREVALAAYAHQALPFE
RLLQQLGPNRSQSHLPVFQVLVTIQNSPEATLQLVDTKIVPIEFERGTAKFDLSFVFTETMNGLAGGIEYNTDLFDAATI
ERMIGHLNSLLLAGLSAPDLSIARLPLLTTAEYARYIAQSRGIASNFPSESCVLTLFEQQVARTPHALAVVQGHASLTYQ
ALDHQANQLAYYLAALGVKPGTGVGLCIERTLMMVVGMMGILRLGAIYVPIDPSYPPERQAYMVANAGLVALVTQDQLAS
ALQAQQPKLAIVALDTDWTAIACAPRVKLPLITADMVLYSIYTSGSTGVPKGTEVTHRNFVNLLNWYITEFNLGSDDRFL
LVTSLSFDLTQKNVFAPLISGGTLYLYDAPQYDPHTITSLIDQHAITVINCTPSAFYPLVETTPDQAIVQLKSLRWLFLG
GEPINLSRFHGWMQSSHYQATIINTYGPTECTDIAASY
>Mature_518_residues
MILATTIKLLLYRYSNQTDMLIGTPVAGRLTTALESIVGFLANTLVLRTSLAGQPSFRVLLQRVREVALAAYAHQALPFE
RLLQQLGPNRSQSHLPVFQVLVTIQNSPEATLQLVDTKIVPIEFERGTAKFDLSFVFTETMNGLAGGIEYNTDLFDAATI
ERMIGHLNSLLLAGLSAPDLSIARLPLLTTAEYARYIAQSRGIASNFPSESCVLTLFEQQVARTPHALAVVQGHASLTYQ
ALDHQANQLAYYLAALGVKPGTGVGLCIERTLMMVVGMMGILRLGAIYVPIDPSYPPERQAYMVANAGLVALVTQDQLAS
ALQAQQPKLAIVALDTDWTAIACAPRVKLPLITADMVLYSIYTSGSTGVPKGTEVTHRNFVNLLNWYITEFNLGSDDRFL
LVTSLSFDLTQKNVFAPLISGGTLYLYDAPQYDPHTITSLIDQHAITVINCTPSAFYPLVETTPDQAIVQLKSLRWLFLG
GEPINLSRFHGWMQSSHYQATIINTYGPTECTDIAASY

Specific function: Activates the 7th to 12th amino acids (Val, D-Val, Trp, D-Leu, Xaa and D-Leu) in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 8th (D-Val), the 10th (D- Leu) an

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 acyl carrier domains [H]

Homologues:

Organism=Escherichia coli, GI1786801, Length=510, Percent_Identity=26.2745098039216, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32564422, Length=169, Percent_Identity=31.3609467455621, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI32564420, Length=169, Percent_Identity=31.3609467455621, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=525, Percent_Identity=24.5714285714286, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648676, Length=323, Percent_Identity=24.1486068111455, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 56689; Mature: 56689

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILATTIKLLLYRYSNQTDMLIGTPVAGRLTTALESIVGFLANTLVLRTSLAGQPSFRVL
CCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LQRVREVALAAYAHQALPFERLLQQLGPNRSQSHLPVFQVLVTIQNSPEATLQLVDTKIV
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEEEECEEEE
PIEFERGTAKFDLSFVFTETMNGLAGGIEYNTDLFDAATIERMIGHLNSLLLAGLSAPDL
EEEECCCCCEEEEEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
SIARLPLLTTAEYARYIAQSRGIASNFPSESCVLTLFEQQVARTPHALAVVQGHASLTYQ
CHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEHH
ALDHQANQLAYYLAALGVKPGTGVGLCIERTLMMVVGMMGILRLGAIYVPIDPSYPPERQ
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCC
AYMVANAGLVALVTQDQLASALQAQQPKLAIVALDTDWTAIACAPRVKLPLITADMVLYS
EEEEECCCEEEEEEHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCEEEHHHEEEE
IYTSGSTGVPKGTEVTHRNFVNLLNWYITEFNLGSDDRFLLVTSLSFDLTQKNVFAPLIS
EECCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCCCHHHHHEEC
GGTLYLYDAPQYDPHTITSLIDQHAITVINCTPSAFYPLVETTPDQAIVQLKSLRWLFLG
CCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHEEEEEEC
GEPINLSRFHGWMQSSHYQATIINTYGPTECTDIAASY
CCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCC
>Mature Secondary Structure
MILATTIKLLLYRYSNQTDMLIGTPVAGRLTTALESIVGFLANTLVLRTSLAGQPSFRVL
CCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LQRVREVALAAYAHQALPFERLLQQLGPNRSQSHLPVFQVLVTIQNSPEATLQLVDTKIV
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEEEECEEEE
PIEFERGTAKFDLSFVFTETMNGLAGGIEYNTDLFDAATIERMIGHLNSLLLAGLSAPDL
EEEECCCCCEEEEEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
SIARLPLLTTAEYARYIAQSRGIASNFPSESCVLTLFEQQVARTPHALAVVQGHASLTYQ
CHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEHH
ALDHQANQLAYYLAALGVKPGTGVGLCIERTLMMVVGMMGILRLGAIYVPIDPSYPPERQ
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCC
AYMVANAGLVALVTQDQLASALQAQQPKLAIVALDTDWTAIACAPRVKLPLITADMVLYS
EEEEECCCEEEEEEHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCEEEHHHEEEE
IYTSGSTGVPKGTEVTHRNFVNLLNWYITEFNLGSDDRFLLVTSLSFDLTQKNVFAPLIS
EECCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCCCHHHHHEEC
GGTLYLYDAPQYDPHTITSLIDQHAITVINCTPSAFYPLVETTPDQAIVQLKSLRWLFLG
CCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHEEEEEEC
GEPINLSRFHGWMQSSHYQATIINTYGPTECTDIAASY
CCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA