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The map label for this gene is dnaN [H]

Identifier: 159898038

GI number: 159898038

Start: 1762878

End: 1764023

Strand: Direct

Name: dnaN [H]

Synonym: Haur_1513

Alternate gene names: 159898038

Gene position: 1762878-1764023 (Clockwise)

Preceding gene: 159898037

Following gene: 159898039

Centisome position: 27.78

GC content: 50.35

Gene sequence:

>1146_bases
ATGCGCCTAGCGTGTTTACAAGAAAATCTAAAGCGTGGTTTAGCCTTGGCAGGCCATGCTGTTGCAGGCCGCTCTAGCTT
GCCTGTACTTGCAAATATTTTGTTGGCAACCGAAAGTGGTCGGCTGAAATTGGCTGGAAATAATCTCGAAATTGGCATTA
CCGCACTCGTCGGCGCAAAAGTCGATGAAGATGGGGCGATTACGATTCCAGCCAAATTATTATCGGATTTAGTTGGCAAT
TTGCCCAATGATACCGTCGAATTAGAAGTTGATCCACGGACGCAAACCTTAACTGTGACATGCCGTGGCACCAAAGCCAA
TATCAAAGGCATCGATGCTGATGAATTTCCGGCGATGGCTGTGCCTGGTAATGAGCCAGTGCTGGCCTATCTACCACCTG
AGCTTTTGCGCAAGGTTGTTAGCCAAGTAGAGGTAGCAGCAGCGGCTGAAAATACCAATCCCAATCCAATTTTGCAAGGT
GTGCTGATTCGTTTGAGTGGCACCCAAGCGACCTTTGCCGCGATTGACGGCTATCGTTTAGCGGTGCGCACGATCGAATT
ACCTGAACCAGCCGCTTACGATACCGAATTGGTGATTCCTTCTAGGGCTATGGCTGAACTGGGCCGCACCCTTGGCGATA
GCGAAACCCCAATTGCCATGAGTATTACGTCCAATGGTGGGCAAGTTATTTTTCATAGCGATACTGTTGATTTTGTTTCG
CGGGTGATCGACGGCAAGTTCCCCGATTACGAGCGCTTTGTGCCACCAGCCAGCAATACCTCAACCCGCAGCGTGATTTC
AACTGCTGAATTTAGCCGCGCTGTCAAACGGATTGCACTGTTCTCGCAGGCTGCTGGCAACATCGTGACCTTGACGATGG
AACGCGGCGATGGCAGCGAACCTGGGCGTTTGCTCTTGAATGCTAATGCTGCAGAAATTGGTGAATCGGTCGAGGCCATT
AGCGCCTTAATTGATGGCGATGATGGCAAATTGGCCTTGAATGTGCGCTATTTGCAAGAAGCACTTGGCGCAATCGACAC
CGATCAAGTAGCTTTGGAAACCCAAACTCCCAACTCACCAGGCGTATTTCGGCCAATTGGCGGCGATGAATACCTGCACT
TGGTGATGCCGATGCGTATTCCCTAA

Upstream 100 bases:

>100_bases
CCAATGGGTCACACCTTAGAACGAGTATGCTATAATAACGAGCTAGTTCATTTTAGCGATGTTATGGTTGCCGCATCGCG
GCAAGGAGACCGAGTTTTCT

Downstream 100 bases:

>100_bases
ATTGGCAATCTATAGGATTTAAGCAAGTAGCTGTGAGGCTGCTTGCTTTTTCGTTATGCGTAAGTTGTGCTCCATGATGA
GTCAGATGAGTCAAGCTCGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRLACLQENLKRGLALAGHAVAGRSSLPVLANILLATESGRLKLAGNNLEIGITALVGAKVDEDGAITIPAKLLSDLVGN
LPNDTVELEVDPRTQTLTVTCRGTKANIKGIDADEFPAMAVPGNEPVLAYLPPELLRKVVSQVEVAAAAENTNPNPILQG
VLIRLSGTQATFAAIDGYRLAVRTIELPEPAAYDTELVIPSRAMAELGRTLGDSETPIAMSITSNGGQVIFHSDTVDFVS
RVIDGKFPDYERFVPPASNTSTRSVISTAEFSRAVKRIALFSQAAGNIVTLTMERGDGSEPGRLLLNANAAEIGESVEAI
SALIDGDDGKLALNVRYLQEALGAIDTDQVALETQTPNSPGVFRPIGGDEYLHLVMPMRIP

Sequences:

>Translated_381_residues
MRLACLQENLKRGLALAGHAVAGRSSLPVLANILLATESGRLKLAGNNLEIGITALVGAKVDEDGAITIPAKLLSDLVGN
LPNDTVELEVDPRTQTLTVTCRGTKANIKGIDADEFPAMAVPGNEPVLAYLPPELLRKVVSQVEVAAAAENTNPNPILQG
VLIRLSGTQATFAAIDGYRLAVRTIELPEPAAYDTELVIPSRAMAELGRTLGDSETPIAMSITSNGGQVIFHSDTVDFVS
RVIDGKFPDYERFVPPASNTSTRSVISTAEFSRAVKRIALFSQAAGNIVTLTMERGDGSEPGRLLLNANAAEIGESVEAI
SALIDGDDGKLALNVRYLQEALGAIDTDQVALETQTPNSPGVFRPIGGDEYLHLVMPMRIP
>Mature_381_residues
MRLACLQENLKRGLALAGHAVAGRSSLPVLANILLATESGRLKLAGNNLEIGITALVGAKVDEDGAITIPAKLLSDLVGN
LPNDTVELEVDPRTQTLTVTCRGTKANIKGIDADEFPAMAVPGNEPVLAYLPPELLRKVVSQVEVAAAAENTNPNPILQG
VLIRLSGTQATFAAIDGYRLAVRTIELPEPAAYDTELVIPSRAMAELGRTLGDSETPIAMSITSNGGQVIFHSDTVDFVS
RVIDGKFPDYERFVPPASNTSTRSVISTAEFSRAVKRIALFSQAAGNIVTLTMERGDGSEPGRLLLNANAAEIGESVEAI
SALIDGDDGKLALNVRYLQEALGAIDTDQVALETQTPNSPGVFRPIGGDEYLHLVMPMRIP

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=381, Percent_Identity=25.4593175853018, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40309; Mature: 40309

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLACLQENLKRGLALAGHAVAGRSSLPVLANILLATESGRLKLAGNNLEIGITALVGAK
CCCHHHHHHHHCCCEEECCEECCCCCCCHHEEEEEEECCCEEEEECCCEEEEEEEEEECE
VDEDGAITIPAKLLSDLVGNLPNDTVELEVDPRTQTLTVTCRGTKANIKGIDADEFPAMA
ECCCCCEEEHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCEEE
VPGNEPVLAYLPPELLRKVVSQVEVAAAAENTNPNPILQGVLIRLSGTQATFAAIDGYRL
CCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEEEECCCEEEEEECCEEE
AVRTIELPEPAAYDTELVIPSRAMAELGRTLGDSETPIAMSITSNGGQVIFHSDTVDFVS
EEEEEECCCCCCCCCEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCHHHHHH
RVIDGKFPDYERFVPPASNTSTRSVISTAEFSRAVKRIALFSQAAGNIVTLTMERGDGSE
HHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
PGRLLLNANAAEIGESVEAISALIDGDDGKLALNVRYLQEALGAIDTDQVALETQTPNSP
CCEEEEECCHHHHHHHHHHHHHHEECCCCEEEEEHHHHHHHHCCCCCCCEEEEECCCCCC
GVFRPIGGDEYLHLVMPMRIP
CEEEECCCCCEEEEEEEECCC
>Mature Secondary Structure
MRLACLQENLKRGLALAGHAVAGRSSLPVLANILLATESGRLKLAGNNLEIGITALVGAK
CCCHHHHHHHHCCCEEECCEECCCCCCCHHEEEEEEECCCEEEEECCCEEEEEEEEEECE
VDEDGAITIPAKLLSDLVGNLPNDTVELEVDPRTQTLTVTCRGTKANIKGIDADEFPAMA
ECCCCCEEEHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCEEE
VPGNEPVLAYLPPELLRKVVSQVEVAAAAENTNPNPILQGVLIRLSGTQATFAAIDGYRL
CCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEEEECCCEEEEEECCEEE
AVRTIELPEPAAYDTELVIPSRAMAELGRTLGDSETPIAMSITSNGGQVIFHSDTVDFVS
EEEEEECCCCCCCCCEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCHHHHHH
RVIDGKFPDYERFVPPASNTSTRSVISTAEFSRAVKRIALFSQAAGNIVTLTMERGDGSE
HHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
PGRLLLNANAAEIGESVEAISALIDGDDGKLALNVRYLQEALGAIDTDQVALETQTPNSP
CCEEEEECCHHHHHHHHHHHHHHEECCCCEEEEEHHHHHHHHCCCCCCCEEEEECCCCCC
GVFRPIGGDEYLHLVMPMRIP
CEEEECCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]