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The map label for this gene is aspS

Identifier: 159897936

GI number: 159897936

Start: 1625411

End: 1627195

Strand: Reverse

Name: aspS

Synonym: Haur_1411

Alternate gene names: 159897936

Gene position: 1627195-1625411 (Counterclockwise)

Preceding gene: 159897940

Following gene: 159897935

Centisome position: 25.64

GC content: 51.43

Gene sequence:

>1785_bases
ATGCTCCGAACTCACACCTGCGGCGAGTTGCGCCGCGACCACATCGACCAAACCGTGACGTTAGCTGGTTGGGTTCATCG
CCGCCGTGATCACGGCACGGTGCTCTTTTTAGATTTGCGTGATCGCTATGGCTTGACCCAAGTGATTGTTGATCCTTCGT
CGAATCCCGAAGCTCGCGCTATGCTCGATAGCATCAAAAATGAGTATGTGATTCAGGTAACTGGGCGGGTGCGCTCACGG
CTTGAGGGCGCAGAAAATGCCAATTTGGCAACTGGCGCGATCGAACTCGAAGTGCAAAGTGCCAAGATTCTCAACACTGC
CAAAACTCCGCCAATCTTGGTGAATCGTGATGGTGGCGAGGATGAAGCGCTGCGCTTGAAATATCGCTATATTGAATTGC
GCCGCGAACGCCAACAGCATATCTTGGGGTTACGCCATCGCACGATCAAATTTATGCGCGATTGGATGGATCGCGAGGGC
TTTTGGGAAATTGAAACCCCGATTTTGATGAAAAGCACGCCCGAAGGCGCACGCGACTATCTTGTGCCAAGTCGCTTACA
TCCAGGCGAATTTTATGCATTGCCCCAATCGCCACAACAACTTAAGCAATTGTTGATGGTTTCGGGGATCGACAAGTATT
TCCAAATTGCGCGTTGTATGCGTGATGAAGATTTACGCGCCGACCGCCAGCCTGAATTCACTCAACTCGACATTGAGATG
AGCTTTGTTGAGCAAGATGATGTACTTGATGTTGTCGAACGCTTGATGACTGAACTGGTGGCCAATGTCACGCCGCACAA
ACGCTTGGTTTCGCCCTTCCCACGGCTCACCTACGCCGACGCGATTGAATATTACGGCTCCGATAAGCCCGATTTGCGCT
ACGACTTGAAATTTGTCAACGTAGGCGAAATCGTCAAAGATAGCCAATTTGGCGTGTTTACTGGTGCACTCAGCAGCGGC
GGCAAAGTTCAAGCCATTCGCTTGCCTGGCTGTGGCAGCTATTCACGTAAGCAAATCGACGATCTGCAAGAAGTTGCCAA
AATTGGTGGAGCCAAAGGCATGGCTTGGATGGCGATCGAAGCTGATGGCAGCGTGCGCTCGCCAATCGCAAAATTCTTCG
AGCAAGCCCAACTTGATCAATTAAAGAGTGCAACGGCGGCTGAAGCTGGCGACTTAATTGTCTATGTTGCCGACAAACCA
GCAGTGGTAGCGGCCTCGTTAGATAAAGTACGGCGCGAAATGGCCAAGCGGCTCGATCTTGCCGACAAAGATCTGATGCA
TTTTGCCTGGGTAATCGACTTCCCAATGTTTGAATACAATGCCGAAAGTGGCGAATGGGATGCCGCTCACCATCCATTCT
GTATGGTCAACCCTGCCGATGTTGAAAAAATGCAGCGCGGCGAGTTTGAAGGCGTGCGGGCCGCCTCATACGACTTGGTA
TGTAACGGCTACGAGTTGGCTTCTGGCTCAATTCGGATTCACGATCGCAGCATTCAGCAATACATTTTTGATCGCTTGCC
CTACAGCCCTGAAGAAATTCAAAAGCGCTTTGGGCATATGTTGGAAGCCTTCGAATATGGCGCACCACCTCATGGCGGTA
TGGCCCCAGGCATCGATCGCTTGGTGATGTTGTTGGCTGATACCGACAACATTCGCGATGTGATTGCCTTCCCCAAAACT
CAACGCGCCGAAGATCTGATGATGAATGCGCCATCATCGGTTGATGCCAAGCAATTACGCGAGCTTGGCTTGCGCTTGGC
CGATGGAGTTGAACCACGCGGTTAA

Upstream 100 bases:

>100_bases
ACGGCTCTAGCCGCGTTATCAACGCTGATTGCACACCGACGTGCTGCTTCTAGGCGTGGCTTTGGCTTTGTTAGTATCCA
AACGCGAGGAGAGCAGTCGT

Downstream 100 bases:

>100_bases
GCATAAATTCCAGCATAAATTGCTCATAGTTGCCGAAACTATGGGCAATTTGTGTTTAAAACCCCAAAACCTGTGATACG
ATAACCTGAGTGATTGAACG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MLRTHTCGELRRDHIDQTVTLAGWVHRRRDHGTVLFLDLRDRYGLTQVIVDPSSNPEARAMLDSIKNEYVIQVTGRVRSR
LEGAENANLATGAIELEVQSAKILNTAKTPPILVNRDGGEDEALRLKYRYIELRRERQQHILGLRHRTIKFMRDWMDREG
FWEIETPILMKSTPEGARDYLVPSRLHPGEFYALPQSPQQLKQLLMVSGIDKYFQIARCMRDEDLRADRQPEFTQLDIEM
SFVEQDDVLDVVERLMTELVANVTPHKRLVSPFPRLTYADAIEYYGSDKPDLRYDLKFVNVGEIVKDSQFGVFTGALSSG
GKVQAIRLPGCGSYSRKQIDDLQEVAKIGGAKGMAWMAIEADGSVRSPIAKFFEQAQLDQLKSATAAEAGDLIVYVADKP
AVVAASLDKVRREMAKRLDLADKDLMHFAWVIDFPMFEYNAESGEWDAAHHPFCMVNPADVEKMQRGEFEGVRAASYDLV
CNGYELASGSIRIHDRSIQQYIFDRLPYSPEEIQKRFGHMLEAFEYGAPPHGGMAPGIDRLVMLLADTDNIRDVIAFPKT
QRAEDLMMNAPSSVDAKQLRELGLRLADGVEPRG

Sequences:

>Translated_594_residues
MLRTHTCGELRRDHIDQTVTLAGWVHRRRDHGTVLFLDLRDRYGLTQVIVDPSSNPEARAMLDSIKNEYVIQVTGRVRSR
LEGAENANLATGAIELEVQSAKILNTAKTPPILVNRDGGEDEALRLKYRYIELRRERQQHILGLRHRTIKFMRDWMDREG
FWEIETPILMKSTPEGARDYLVPSRLHPGEFYALPQSPQQLKQLLMVSGIDKYFQIARCMRDEDLRADRQPEFTQLDIEM
SFVEQDDVLDVVERLMTELVANVTPHKRLVSPFPRLTYADAIEYYGSDKPDLRYDLKFVNVGEIVKDSQFGVFTGALSSG
GKVQAIRLPGCGSYSRKQIDDLQEVAKIGGAKGMAWMAIEADGSVRSPIAKFFEQAQLDQLKSATAAEAGDLIVYVADKP
AVVAASLDKVRREMAKRLDLADKDLMHFAWVIDFPMFEYNAESGEWDAAHHPFCMVNPADVEKMQRGEFEGVRAASYDLV
CNGYELASGSIRIHDRSIQQYIFDRLPYSPEEIQKRFGHMLEAFEYGAPPHGGMAPGIDRLVMLLADTDNIRDVIAFPKT
QRAEDLMMNAPSSVDAKQLRELGLRLADGVEPRG
>Mature_594_residues
MLRTHTCGELRRDHIDQTVTLAGWVHRRRDHGTVLFLDLRDRYGLTQVIVDPSSNPEARAMLDSIKNEYVIQVTGRVRSR
LEGAENANLATGAIELEVQSAKILNTAKTPPILVNRDGGEDEALRLKYRYIELRRERQQHILGLRHRTIKFMRDWMDREG
FWEIETPILMKSTPEGARDYLVPSRLHPGEFYALPQSPQQLKQLLMVSGIDKYFQIARCMRDEDLRADRQPEFTQLDIEM
SFVEQDDVLDVVERLMTELVANVTPHKRLVSPFPRLTYADAIEYYGSDKPDLRYDLKFVNVGEIVKDSQFGVFTGALSSG
GKVQAIRLPGCGSYSRKQIDDLQEVAKIGGAKGMAWMAIEADGSVRSPIAKFFEQAQLDQLKSATAAEAGDLIVYVADKP
AVVAASLDKVRREMAKRLDLADKDLMHFAWVIDFPMFEYNAESGEWDAAHHPFCMVNPADVEKMQRGEFEGVRAASYDLV
CNGYELASGSIRIHDRSIQQYIFDRLPYSPEEIQKRFGHMLEAFEYGAPPHGGMAPGIDRLVMLLADTDNIRDVIAFPKT
QRAEDLMMNAPSSVDAKQLRELGLRLADGVEPRG

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=597, Percent_Identity=41.8760469011725, Blast_Score=451, Evalue=1e-126,
Organism=Homo sapiens, GI5031815, Length=260, Percent_Identity=26.9230769230769, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI194272210, Length=260, Percent_Identity=26.9230769230769, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4758762, Length=291, Percent_Identity=28.8659793814433, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI45439306, Length=301, Percent_Identity=25.9136212624585, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788173, Length=596, Percent_Identity=46.6442953020134, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI1790571, Length=279, Percent_Identity=26.5232974910394, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789256, Length=280, Percent_Identity=25.7142857142857, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=597, Percent_Identity=35.1758793969849, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI71984122, Length=309, Percent_Identity=27.5080906148867, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71994340, Length=258, Percent_Identity=25.968992248062, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=258, Percent_Identity=25.968992248062, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=258, Percent_Identity=25.968992248062, Blast_Score=89, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6325153, Length=646, Percent_Identity=32.1981424148607, Blast_Score=287, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6323011, Length=310, Percent_Identity=30, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=287, Percent_Identity=27.1777003484321, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=260, Percent_Identity=21.9230769230769, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584738, Length=606, Percent_Identity=36.3036303630363, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI17136276, Length=244, Percent_Identity=27.8688524590164, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24640849, Length=256, Percent_Identity=26.171875, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24640851, Length=256, Percent_Identity=26.171875, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921528, Length=288, Percent_Identity=27.0833333333333, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_HERA2 (A9B392)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001544183.1
- ProteinModelPortal:   A9B392
- SMR:   A9B392
- GeneID:   5733319
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_1411
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   HAUR316274:HAUR_1411-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67264; Mature: 67264

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTHTCGELRRDHIDQTVTLAGWVHRRRDHGTVLFLDLRDRYGLTQVIVDPSSNPEARA
CCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCCHHHH
MLDSIKNEYVIQVTGRVRSRLEGAENANLATGAIELEVQSAKILNTAKTPPILVNRDGGE
HHHHCCCCEEEEEHHHHHHHHCCCCCCCEEEEEEEEEEECHHHHHCCCCCCEEEECCCCC
DEALRLKYRYIELRRERQQHILGLRHRTIKFMRDWMDREGFWEIETPILMKSTPEGARDY
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCHHHC
LVPSRLHPGEFYALPQSPQQLKQLLMVSGIDKYFQIARCMRDEDLRADRQPEFTQLDIEM
CCCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFVEQDDVLDVVERLMTELVANVTPHKRLVSPFPRLTYADAIEYYGSDKPDLRYDLKFVN
EECCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEEE
VGEIVKDSQFGVFTGALSSGGKVQAIRLPGCGSYSRKQIDDLQEVAKIGGAKGMAWMAIE
HHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEE
ADGSVRSPIAKFFEQAQLDQLKSATAAEAGDLIVYVADKPAVVAASLDKVRREMAKRLDL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHCCC
ADKDLMHFAWVIDFPMFEYNAESGEWDAAHHPFCMVNPADVEKMQRGEFEGVRAASYDLV
CHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCEEECEEEE
CNGYELASGSIRIHDRSIQQYIFDRLPYSPEEIQKRFGHMLEAFEYGAPPHGGMAPGIDR
ECCEEECCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LVMLLADTDNIRDVIAFPKTQRAEDLMMNAPSSVDAKQLRELGLRLADGVEPRG
HEEHEECCCCCHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHCCHHHCCCCCCC
>Mature Secondary Structure
MLRTHTCGELRRDHIDQTVTLAGWVHRRRDHGTVLFLDLRDRYGLTQVIVDPSSNPEARA
CCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCCHHHH
MLDSIKNEYVIQVTGRVRSRLEGAENANLATGAIELEVQSAKILNTAKTPPILVNRDGGE
HHHHCCCCEEEEEHHHHHHHHCCCCCCCEEEEEEEEEEECHHHHHCCCCCCEEEECCCCC
DEALRLKYRYIELRRERQQHILGLRHRTIKFMRDWMDREGFWEIETPILMKSTPEGARDY
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCHHHC
LVPSRLHPGEFYALPQSPQQLKQLLMVSGIDKYFQIARCMRDEDLRADRQPEFTQLDIEM
CCCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFVEQDDVLDVVERLMTELVANVTPHKRLVSPFPRLTYADAIEYYGSDKPDLRYDLKFVN
EECCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEEE
VGEIVKDSQFGVFTGALSSGGKVQAIRLPGCGSYSRKQIDDLQEVAKIGGAKGMAWMAIE
HHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEE
ADGSVRSPIAKFFEQAQLDQLKSATAAEAGDLIVYVADKPAVVAASLDKVRREMAKRLDL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHCCC
ADKDLMHFAWVIDFPMFEYNAESGEWDAAHHPFCMVNPADVEKMQRGEFEGVRAASYDLV
CHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCEEECEEEE
CNGYELASGSIRIHDRSIQQYIFDRLPYSPEEIQKRFGHMLEAFEYGAPPHGGMAPGIDR
ECCEEECCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LVMLLADTDNIRDVIAFPKTQRAEDLMMNAPSSVDAKQLRELGLRLADGVEPRG
HEEHEECCCCCHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA