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The map label for this gene is vfr [H]

Identifier: 159897826

GI number: 159897826

Start: 1505999

End: 1506667

Strand: Reverse

Name: vfr [H]

Synonym: Haur_1297

Alternate gene names: 159897826

Gene position: 1506667-1505999 (Counterclockwise)

Preceding gene: 159897836

Following gene: 159897823

Centisome position: 23.74

GC content: 50.07

Gene sequence:

>669_bases
ATGGATCGAAATGTTTTATTTGCCCATGTCAGTTTCACTCGCGATCTATCGCCAACGATTATTGCTGCGCTGAGCAGCCA
TGCCAATGAACAAACCGTCGAGCGTGGTCAGATTATCACGCTGGAGGGCGACCCCGCCGTAGCAATGTATCTGGTGTATA
GCGGGCGAATTAAGGTTGTGCGTCATTCAATCGAGGGCCGCGAGCATATTTTACACGTAGTTGAGGCCTACGATCATTTT
AATACAGTCCCGATGTTTCGTGAAGCCCGCTGCCCAGCCACCAGTGAAGCCTTAGTTACCAGCCAATTATTGGCAATTCC
GCGTGAACATATCCGCCAAGTCGCCCAGCAACACCCTGAATTAGCCTTGGCAATGTTGGGCGAATTCGCGAACCGTTTGC
ATAATTTGGTTGGCTTAATTGAAAATTTGGCCTTGCATACGGTCAACGGGCGTTTGGCGCGTTTATTGTTGCAACAAGCT
GCATTAGCCGAACAAAATCCCAATCTTGCACCTTTAACCCAAGCTGAAATGGCCGCGCATATTGGCTCAGTGCGTGAGAT
GGTCGGGCGAGCCTTGAAGGCCTTTGATGCCCAAGGCTTAATTCGGCTTGAGCGCGGCATAATCGAAATTATCGATCCCC
AAGGTTTAGCTGCCCAAGCTGAGCTTTAG

Upstream 100 bases:

>100_bases
CGGAAAGGTACCAACAATCAATTGCGAAAGTAATGCATGCATTGGGCAAATTCCTTGTACTATGGTGGCAGTCCAGTTCT
AGTGCAACGAGGATACGCTG

Downstream 100 bases:

>100_bases
TTAATGGCTGGCTGCTTGGGGCAAAGCGAATTGCAAACGAACGGCTAATCGCTTCGGTTGGGCAGGCAATTTCGCAGCGA
TCGCAAAAGGTACAGGCTTC

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MDRNVLFAHVSFTRDLSPTIIAALSSHANEQTVERGQIITLEGDPAVAMYLVYSGRIKVVRHSIEGREHILHVVEAYDHF
NTVPMFREARCPATSEALVTSQLLAIPREHIRQVAQQHPELALAMLGEFANRLHNLVGLIENLALHTVNGRLARLLLQQA
ALAEQNPNLAPLTQAEMAAHIGSVREMVGRALKAFDAQGLIRLERGIIEIIDPQGLAAQAEL

Sequences:

>Translated_222_residues
MDRNVLFAHVSFTRDLSPTIIAALSSHANEQTVERGQIITLEGDPAVAMYLVYSGRIKVVRHSIEGREHILHVVEAYDHF
NTVPMFREARCPATSEALVTSQLLAIPREHIRQVAQQHPELALAMLGEFANRLHNLVGLIENLALHTVNGRLARLLLQQA
ALAEQNPNLAPLTQAEMAAHIGSVREMVGRALKAFDAQGLIRLERGIIEIIDPQGLAAQAEL
>Mature_222_residues
MDRNVLFAHVSFTRDLSPTIIAALSSHANEQTVERGQIITLEGDPAVAMYLVYSGRIKVVRHSIEGREHILHVVEAYDHF
NTVPMFREARCPATSEALVTSQLLAIPREHIRQVAQQHPELALAMLGEFANRLHNLVGLIENLALHTVNGRLARLLLQQA
ALAEQNPNLAPLTQAEMAAHIGSVREMVGRALKAFDAQGLIRLERGIIEIIDPQGLAAQAEL

Specific function: Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=165, Percent_Identity=28.4848484848485, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24472; Mature: 24472

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRNVLFAHVSFTRDLSPTIIAALSSHANEQTVERGQIITLEGDPAVAMYLVYSGRIKVV
CCCCEEEEEEEECCCCCHHHHHHHHHCCCHHHHHCCCEEEECCCCCEEEEEEECCCCEEH
RHSIEGREHILHVVEAYDHFNTVPMFREARCPATSEALVTSQLLAIPREHIRQVAQQHPE
HHHHCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCHH
LALAMLGEFANRLHNLVGLIENLALHTVNGRLARLLLQQAALAEQNPNLAPLTQAEMAAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IGSVREMVGRALKAFDAQGLIRLERGIIEIIDPQGLAAQAEL
HHHHHHHHHHHHHHHCCCCHHHHHHCHHEEECCCCCCCCCCC
>Mature Secondary Structure
MDRNVLFAHVSFTRDLSPTIIAALSSHANEQTVERGQIITLEGDPAVAMYLVYSGRIKVV
CCCCEEEEEEEECCCCCHHHHHHHHHCCCHHHHHCCCEEEECCCCCEEEEEEECCCCEEH
RHSIEGREHILHVVEAYDHFNTVPMFREARCPATSEALVTSQLLAIPREHIRQVAQQHPE
HHHHCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCHH
LALAMLGEFANRLHNLVGLIENLALHTVNGRLARLLLQQAALAEQNPNLAPLTQAEMAAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IGSVREMVGRALKAFDAQGLIRLERGIIEIIDPQGLAAQAEL
HHHHHHHHHHHHHHHCCCCHHHHHHCHHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002577; 10984043 [H]