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The map label for this gene is cca [H]

Identifier: 159896797

GI number: 159896797

Start: 310748

End: 312301

Strand: Reverse

Name: cca [H]

Synonym: Haur_0264

Alternate gene names: 159896797

Gene position: 312301-310748 (Counterclockwise)

Preceding gene: 159896799

Following gene: 159896795

Centisome position: 4.92

GC content: 53.6

Gene sequence:

>1554_bases
ATGAAGATTAGTCATATTCAGTTGCCCCAAAGCTCGGCCTATCTTAGTTTACGCCAAGCCTTGAGCGTGCTTAAACACCT
TTTAGGTTCTCAACCAGCCTGGGTCGTCGGCGGTACATTGCGCGATTTAGTGATTGGCCGCCCGTTGCATGATGTCGATT
TGGTGGTCAATCAACCAGCGATTGGCTTAGCCCAAAGCCTTGCCAAGCAACTCAACGCCAGTTTGGTGGTGCTCGATGCT
GAGCGTGATATTGGGCGGGTGGTCTTGGCCGATGGCAAGTATCTTGATCTTGCTCATTTGCGGGCTGCTGATTTAACCAG
CGATTTGACCGACCGCGATTTTACGATTAATGCCATGGCCGCGCCGCTTTACGAGCAAGCGCAACTTGGCCAAATCATCG
ATCCTCATGCTGGATTGAACGATATTGCGACTGGTACGCTGCGCATGGTGCAAGCCAATGCCCTAGCCAACGATCCATTG
CGTATGCTGCGAGCGGTGCGGATCGCTGCTCAACTCGGCTGGCAACTTGATCCAGCAACCGATCAGGCGATCATAGCCCA
CGCCGAATTGATCAACCAAGTGGCGGTTGAACGGGTACGAGTTGAGCTATTGGCAATTTTAGCCACTCGTTGGAGTGCTA
GCTGGCTGCGTTATTTAGATCGGGTTAATCTTTTGCCAAGCTTAATTCCTGAGATTCGCCCAATGTACCACCACACCCAG
CCCAATATTCATTTTTTGGATGTGTGGGAGCATTCGCTCGAAACGATTACTGCTGGCGAATGGCTGCTTGCCCAGTTGGA
ACATGATCAACAGCCAGTGCCACCAGCACCGCCAGCACTTGCCGACGATTGGCATCCACCAGCCTTTTTTCAGCAACCCG
CAACCTTGCGCATGCTACCCAACTTGACCCTGCAACTGCAATGGGCCGAGCAGGTTGTTGCCCAAATGCAACAGCCAATT
GGCCACACTGCCAGCAATCGCGCCCTCTGGAAAATGGCGATTCTGCTGCACGATTGTGCGAAGCCAGCGACACGAGTTGA
TAAGCCCGATGGCAGCGTCTCGTTTCATGAACATCAAACGATTGGGGCAGACATTGCCCGAGCAGTGTATCAGCGGCTGC
GATTTAGCAACCAAGAGATTGAGTATATGTGCAAGGTGATTGCGGGGCATATGCGGCCTGGTCAGCTTTACTCGCAGCCT
GAAACCACGGCCAAAGCGGCCTATCGCTTCTTTCGCGATTTAAAAGAAAGCGCCGTCGATACCTTACTGCACGCCTTGGC
CGACCATATGGCGACGCGCGGGCCGTTGCTCCAAGAACATCATTGGGCCTACCAAGCCGCTTGGACAGATGCGATGCTTG
GGTTTTATTGGGATGCTGAGGCCGAACAAAAACGTGTGCCCTTACTCGATGGACACACGCTGATGCAAGAGCTAGGTTTA
GCAGCCGGCCCACAGATTGGGCTGTTGCTTGAGGCAATTCGTGAAGCTCAAGCGGCTGGCGAAGTGACCGACCGTGAGAG
CGCCTTAGCGTTGGCGCGTCGGCTTCATACGTAA

Upstream 100 bases:

>100_bases
GTTTGCGTTACGGTCAAACTCATTGCCTTTCGTCCTTTCGCCATTGGTGGGTTCAATTATCCCACAATTATAGAGCATAG
CTGCCCAACGTGAGTTTTGT

Downstream 100 bases:

>100_bases
TTGTGGCTCGTCGGAATCATCCTCGACCACTGGCGTTGAGCGTGAGACATACGGCAATGCGGCTAATGCCACAAGCAGGA
GCGCAACAAAAAATAGTGTC

Product: polynucleotide adenylyltransferase/metal dependent phosphohydrolase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKISHIQLPQSSAYLSLRQALSVLKHLLGSQPAWVVGGTLRDLVIGRPLHDVDLVVNQPAIGLAQSLAKQLNASLVVLDA
ERDIGRVVLADGKYLDLAHLRAADLTSDLTDRDFTINAMAAPLYEQAQLGQIIDPHAGLNDIATGTLRMVQANALANDPL
RMLRAVRIAAQLGWQLDPATDQAIIAHAELINQVAVERVRVELLAILATRWSASWLRYLDRVNLLPSLIPEIRPMYHHTQ
PNIHFLDVWEHSLETITAGEWLLAQLEHDQQPVPPAPPALADDWHPPAFFQQPATLRMLPNLTLQLQWAEQVVAQMQQPI
GHTASNRALWKMAILLHDCAKPATRVDKPDGSVSFHEHQTIGADIARAVYQRLRFSNQEIEYMCKVIAGHMRPGQLYSQP
ETTAKAAYRFFRDLKESAVDTLLHALADHMATRGPLLQEHHWAYQAAWTDAMLGFYWDAEAEQKRVPLLDGHTLMQELGL
AAGPQIGLLLEAIREAQAAGEVTDRESALALARRLHT

Sequences:

>Translated_517_residues
MKISHIQLPQSSAYLSLRQALSVLKHLLGSQPAWVVGGTLRDLVIGRPLHDVDLVVNQPAIGLAQSLAKQLNASLVVLDA
ERDIGRVVLADGKYLDLAHLRAADLTSDLTDRDFTINAMAAPLYEQAQLGQIIDPHAGLNDIATGTLRMVQANALANDPL
RMLRAVRIAAQLGWQLDPATDQAIIAHAELINQVAVERVRVELLAILATRWSASWLRYLDRVNLLPSLIPEIRPMYHHTQ
PNIHFLDVWEHSLETITAGEWLLAQLEHDQQPVPPAPPALADDWHPPAFFQQPATLRMLPNLTLQLQWAEQVVAQMQQPI
GHTASNRALWKMAILLHDCAKPATRVDKPDGSVSFHEHQTIGADIARAVYQRLRFSNQEIEYMCKVIAGHMRPGQLYSQP
ETTAKAAYRFFRDLKESAVDTLLHALADHMATRGPLLQEHHWAYQAAWTDAMLGFYWDAEAEQKRVPLLDGHTLMQELGL
AAGPQIGLLLEAIREAQAAGEVTDRESALALARRLHT
>Mature_517_residues
MKISHIQLPQSSAYLSLRQALSVLKHLLGSQPAWVVGGTLRDLVIGRPLHDVDLVVNQPAIGLAQSLAKQLNASLVVLDA
ERDIGRVVLADGKYLDLAHLRAADLTSDLTDRDFTINAMAAPLYEQAQLGQIIDPHAGLNDIATGTLRMVQANALANDPL
RMLRAVRIAAQLGWQLDPATDQAIIAHAELINQVAVERVRVELLAILATRWSASWLRYLDRVNLLPSLIPEIRPMYHHTQ
PNIHFLDVWEHSLETITAGEWLLAQLEHDQQPVPPAPPALADDWHPPAFFQQPATLRMLPNLTLQLQWAEQVVAQMQQPI
GHTASNRALWKMAILLHDCAKPATRVDKPDGSVSFHEHQTIGADIARAVYQRLRFSNQEIEYMCKVIAGHMRPGQLYSQP
ETTAKAAYRFFRDLKESAVDTLLHALADHMATRGPLLQEHHWAYQAAWTDAMLGFYWDAEAEQKRVPLLDGHTLMQELGL
AAGPQIGLLLEAIREAQAAGEVTDRESALALARRLHT

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=250, Percent_Identity=30.4, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 57529; Mature: 57529

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISHIQLPQSSAYLSLRQALSVLKHLLGSQPAWVVGGTLRDLVIGRPLHDVDLVVNQPA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHCCCCCHHHEEECCCH
IGLAQSLAKQLNASLVVLDAERDIGRVVLADGKYLDLAHLRAADLTSDLTDRDFTINAMA
HHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCEEEHHHH
APLYEQAQLGQIIDPHAGLNDIATGTLRMVQANALANDPLRMLRAVRIAAQLGWQLDPAT
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
DQAIIAHAELINQVAVERVRVELLAILATRWSASWLRYLDRVNLLPSLIPEIRPMYHHTQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PNIHFLDVWEHSLETITAGEWLLAQLEHDQQPVPPAPPALADDWHPPAFFQQPATLRMLP
CCEEEEHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEECC
NLTLQLQWAEQVVAQMQQPIGHTASNRALWKMAILLHDCAKPATRVDKPDGSVSFHEHQT
CCEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCEEHHHHH
IGADIARAVYQRLRFSNQEIEYMCKVIAGHMRPGQLYSQPETTAKAAYRFFRDLKESAVD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLLHALADHMATRGPLLQEHHWAYQAAWTDAMLGFYWDAEAEQKRVPLLDGHTLMQELGL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHCCC
AAGPQIGLLLEAIREAQAAGEVTDRESALALARRLHT
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKISHIQLPQSSAYLSLRQALSVLKHLLGSQPAWVVGGTLRDLVIGRPLHDVDLVVNQPA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHCCCCCHHHEEECCCH
IGLAQSLAKQLNASLVVLDAERDIGRVVLADGKYLDLAHLRAADLTSDLTDRDFTINAMA
HHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCEEEHHHH
APLYEQAQLGQIIDPHAGLNDIATGTLRMVQANALANDPLRMLRAVRIAAQLGWQLDPAT
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
DQAIIAHAELINQVAVERVRVELLAILATRWSASWLRYLDRVNLLPSLIPEIRPMYHHTQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PNIHFLDVWEHSLETITAGEWLLAQLEHDQQPVPPAPPALADDWHPPAFFQQPATLRMLP
CCEEEEHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEECC
NLTLQLQWAEQVVAQMQQPIGHTASNRALWKMAILLHDCAKPATRVDKPDGSVSFHEHQT
CCEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCEEHHHHH
IGADIARAVYQRLRFSNQEIEYMCKVIAGHMRPGQLYSQPETTAKAAYRFFRDLKESAVD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLLHALADHMATRGPLLQEHHWAYQAAWTDAMLGFYWDAEAEQKRVPLLDGHTLMQELGL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHCCC
AAGPQIGLLLEAIREAQAAGEVTDRESALALARRLHT
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]