Click here to switch to the map view.

The map label for this gene is lexA

Identifier: 159896684

GI number: 159896684

Start: 182016

End: 182660

Strand: Direct

Name: lexA

Synonym: Haur_0151

Alternate gene names: 159896684

Gene position: 182016-182660 (Clockwise)

Preceding gene: 159896676

Following gene: 159896687

Centisome position: 2.87

GC content: 51.47

Gene sequence:

>645_bases
ATGAGCGGATTATCGCAACGCCAACAACGGATCTATGATTACATCAAGCAATTTATTCGCACAAATGGCTATGCTCCGGC
GATTCGCGATATTCAACGCGAGTTAAGTATTTCATCAACCTCGGTGGTTGCATATAACTTACGCGCCCTCGAAAGCAAAG
GCCATATCCGCCGCGAAGGTAATATCTCGCGAGCTATCGAATTGATCAATGCTGAGCAACCACTGCCAACCGTGCTTGGT
GGCCAACGCGTGCCAGTGCTGGGCGTAATTGCCGCTGGTCAGCCTATTCCAGTGCCCAACGATTCGGCCAACTCCGACGA
TTCGGTGTTAGTGCCCGAGGAAATTGTTGGCAACGATAAGCTGGGCGATGTGTATGCCTTGCGAGTCAAAGGCTATTCGA
TGGTCGATGCCTTGATTGCCGATGGCGATATTGTGCTCTTGCGCTATCAGGCAACCGCCGAAAATGGCGAGATGGTTGCA
GCCCGCATTCGCGATGAAAACGAAGTTACTTTGAAACGGATCTACTGGGAAGGTGACCGCGTGCGGTTGCAACCAGCCAA
CGTTACCATGGAAGCAATGTACTATCCATCAACCAATGTGGAAGTGCAAGGCCGTGTGGTTGGGGTGATTCGTAACCTCG
GCTAA

Upstream 100 bases:

>100_bases
TGACAGAACATATGTATTTTTCTATAATAAAGACTAGAACGTCTATTCGTGTATGGCTGAATACCCACAGCCTCGTCGGC
CCTGAAAGGAAGGCGCTTCA

Downstream 100 bases:

>100_bases
AAGCTGAACTTCACGTGATAAACCCAAGGGACGGAATTATTTCCGTCCCTTTTTGTTGCTGCGTTGGTTGATCTTCTCTT
GGGTTTCGATCAATTTGGCT

Product: LexA family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSGLSQRQQRIYDYIKQFIRTNGYAPAIRDIQRELSISSTSVVAYNLRALESKGHIRREGNISRAIELINAEQPLPTVLG
GQRVPVLGVIAAGQPIPVPNDSANSDDSVLVPEEIVGNDKLGDVYALRVKGYSMVDALIADGDIVLLRYQATAENGEMVA
ARIRDENEVTLKRIYWEGDRVRLQPANVTMEAMYYPSTNVEVQGRVVGVIRNLG

Sequences:

>Translated_214_residues
MSGLSQRQQRIYDYIKQFIRTNGYAPAIRDIQRELSISSTSVVAYNLRALESKGHIRREGNISRAIELINAEQPLPTVLG
GQRVPVLGVIAAGQPIPVPNDSANSDDSVLVPEEIVGNDKLGDVYALRVKGYSMVDALIADGDIVLLRYQATAENGEMVA
ARIRDENEVTLKRIYWEGDRVRLQPANVTMEAMYYPSTNVEVQGRVVGVIRNLG
>Mature_213_residues
SGLSQRQQRIYDYIKQFIRTNGYAPAIRDIQRELSISSTSVVAYNLRALESKGHIRREGNISRAIELINAEQPLPTVLGG
QRVPVLGVIAAGQPIPVPNDSANSDDSVLVPEEIVGNDKLGDVYALRVKGYSMVDALIADGDIVLLRYQATAENGEMVAA
RIRDENEVTLKRIYWEGDRVRLQPANVTMEAMYYPSTNVEVQGRVVGVIRNLG

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=217, Percent_Identity=31.3364055299539, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_HERA2 (A9B5H8)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001542931.1
- ProteinModelPortal:   A9B5H8
- SMR:   A9B5H8
- MEROPS:   S24.001
- GeneID:   5732060
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_0151
- HOGENOM:   HBG679610
- OMA:   VIAMTDE
- ProtClustDB:   CLSK935549
- BioCyc:   HAUR316274:HAUR_0151-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 23653; Mature: 23522

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: ACT_SITE 133-133 ACT_SITE 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLSQRQQRIYDYIKQFIRTNGYAPAIRDIQRELSISSTSVVAYNLRALESKGHIRREG
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEECCC
NISRAIELINAEQPLPTVLGGQRVPVLGVIAAGQPIPVPNDSANSDDSVLVPEEIVGNDK
CHHHHHHHHCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCHHHCCCCC
LGDVYALRVKGYSMVDALIADGDIVLLRYQATAENGEMVAARIRDENEVTLKRIYWEGDR
CCCEEEEEECCCHHHHHHHCCCCEEEEEEEECCCCCCEEEEEECCCCCEEEEEEEECCCE
VRLQPANVTMEAMYYPSTNVEVQGRVVGVIRNLG
EEEEECCEEEEEEEECCCCEEECCEEEEEEECCC
>Mature Secondary Structure 
SGLSQRQQRIYDYIKQFIRTNGYAPAIRDIQRELSISSTSVVAYNLRALESKGHIRREG
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEECCC
NISRAIELINAEQPLPTVLGGQRVPVLGVIAAGQPIPVPNDSANSDDSVLVPEEIVGNDK
CHHHHHHHHCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCHHHCCCCC
LGDVYALRVKGYSMVDALIADGDIVLLRYQATAENGEMVAARIRDENEVTLKRIYWEGDR
CCCEEEEEECCCHHHHHHHCCCCEEEEEEEECCCCCCEEEEEECCCCCEEEEEEEECCCE
VRLQPANVTMEAMYYPSTNVEVQGRVVGVIRNLG
EEEEECCEEEEEEEECCCCEEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA