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The map label for this gene is gabT [C]

Identifier: 159896672

GI number: 159896672

Start: 171868

End: 173226

Strand: Reverse

Name: gabT [C]

Synonym: Haur_0139

Alternate gene names: 159896672

Gene position: 173226-171868 (Counterclockwise)

Preceding gene: 159896677

Following gene: 159896671

Centisome position: 2.73

GC content: 52.54

Gene sequence:

>1359_bases
ATGTCGCCTATCAACCTGTTTAAGGAGTTCCCCGTGACTGATCTGCTGACGACGATTCCTGGCCCCCGCGCAACTGCCCT
AGTTGATCGCGATACGGCGGTAATGGCCCCATGTAGTGGGCGGGTCTATCCTTTTGTGATGGATCGTGGCCTTGGTTGTG
AAGTGTGGGATGTTGATGGTAATCGCTATCTCGATTTGAATGCGGGGATTGCGGTGGTTTCAACGGGTCATAGCCACCCG
CGGCTGGTGCAAGCCCTACAAGATCAGGTATCCAAGTTTATCCATATGGCTGGAACTGATTTTTATAATGAGCCAATGGT
CAAGGCTGCCGAAAAAATCACGTCGTTGATGCCTGGCTCTGGCGAGTGGCAAGTATTTTTCGCCAATAGCGGCACTGAAT
CAGTTGAAGCAGCAATCAAATTGGCGCGATATTACACCAAACGCCAAAATGTCATCGGCTTCTATAGCTCGTTCCATGGC
CGCTCATATGGTAGCTTATCGATCACCGCCTCGAAGCCCTACCAACGCAAAGGTTTTTTCCCCTTGATGCCTGGTGTATT
CCACGCCTTCTATCCCAACCCCTATCGCACACCTTGGGGCGTTGAGCCAGAGCGCGTTGCCGAAATCTGCTTGGATTTTA
TCGAAAAGACCTTGTTTACCACCACCACCCCTGCCGACGATGTAGCGGCAATTATCGCCGAGCCAATTCAAGGTGAAGGC
GGCTATGTTGTGCCAGCCCCAGGCTTCTGGCAAGGTTTGCGCGATCTCTGCGATCGCCACGGCATTTTGTTGATCGCCGA
TGAAGTGCAAAGCGGTGTTGGCCGAACTGGCAAAATGTGGGCAGTTGAATATGAAGGCATTGTGCCCGACATCATCACCA
GCGCCAAAGGCATTGGCTCAGGCGTGCCAGTGGGCGCGATGGTCGCCCGCAAAGAAATTAGCAGCGTTTGGAAACCAGGC
GCACACGGCAATACCTATGGTGGCAACGCCTTGGCCATGCGAGCCGTCTACGAAACCTTGTGCCTCGCCGAAGAAGAATT
GATGGCTAACGCCAACGAAGTTGGTGGCTACTTCAAACAACGGCTGCACGAACTCGAAGATCGCTACGAATGTATCGGCG
ATGTACGGGGCCGTGGTTTGATGATCGGCATGGAATTCATCAAAGATAACGTCAACAAAGATCCGCATGGCGATCTATCC
AACGCTGTGATGGAAGAATCGTTCCGCCGTGGTATGTTGCTGCTGACCTGTGGCAAATCGACCATCCGCTTCTGCCCGCC
ATTGGTGCTGACCAAAGATCAAGTTGATGAAGGTATCAATTTGTTGAACGACACCTTGCAAGCCCTCGGCGCAAAGTAA

Upstream 100 bases:

>100_bases
TTCTGCCTTTTGACTTTTGATTTACCTTTGACGCACTAGAATGGCTCGGATAGAATGGGTAGGAATACCTATCACTGTGC
CAACGGTGGGTATTTTGGCA

Downstream 100 bases:

>100_bases
TTATCAATCAATACCTGCGAAGCGTAGCGATACTACGCTTCGCAGAGTTTTGCTGAATCACTTATCAAAACCAATAGTAT
AGCCATACTTCAAAAGGATG

Product: class III aminotransferase

Products: succinate semialdehyde; L-glutamate

Alternate protein names: ORF B [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSPINLFKEFPVTDLLTTIPGPRATALVDRDTAVMAPCSGRVYPFVMDRGLGCEVWDVDGNRYLDLNAGIAVVSTGHSHP
RLVQALQDQVSKFIHMAGTDFYNEPMVKAAEKITSLMPGSGEWQVFFANSGTESVEAAIKLARYYTKRQNVIGFYSSFHG
RSYGSLSITASKPYQRKGFFPLMPGVFHAFYPNPYRTPWGVEPERVAEICLDFIEKTLFTTTTPADDVAAIIAEPIQGEG
GYVVPAPGFWQGLRDLCDRHGILLIADEVQSGVGRTGKMWAVEYEGIVPDIITSAKGIGSGVPVGAMVARKEISSVWKPG
AHGNTYGGNALAMRAVYETLCLAEEELMANANEVGGYFKQRLHELEDRYECIGDVRGRGLMIGMEFIKDNVNKDPHGDLS
NAVMEESFRRGMLLLTCGKSTIRFCPPLVLTKDQVDEGINLLNDTLQALGAK

Sequences:

>Translated_452_residues
MSPINLFKEFPVTDLLTTIPGPRATALVDRDTAVMAPCSGRVYPFVMDRGLGCEVWDVDGNRYLDLNAGIAVVSTGHSHP
RLVQALQDQVSKFIHMAGTDFYNEPMVKAAEKITSLMPGSGEWQVFFANSGTESVEAAIKLARYYTKRQNVIGFYSSFHG
RSYGSLSITASKPYQRKGFFPLMPGVFHAFYPNPYRTPWGVEPERVAEICLDFIEKTLFTTTTPADDVAAIIAEPIQGEG
GYVVPAPGFWQGLRDLCDRHGILLIADEVQSGVGRTGKMWAVEYEGIVPDIITSAKGIGSGVPVGAMVARKEISSVWKPG
AHGNTYGGNALAMRAVYETLCLAEEELMANANEVGGYFKQRLHELEDRYECIGDVRGRGLMIGMEFIKDNVNKDPHGDLS
NAVMEESFRRGMLLLTCGKSTIRFCPPLVLTKDQVDEGINLLNDTLQALGAK
>Mature_451_residues
SPINLFKEFPVTDLLTTIPGPRATALVDRDTAVMAPCSGRVYPFVMDRGLGCEVWDVDGNRYLDLNAGIAVVSTGHSHPR
LVQALQDQVSKFIHMAGTDFYNEPMVKAAEKITSLMPGSGEWQVFFANSGTESVEAAIKLARYYTKRQNVIGFYSSFHGR
SYGSLSITASKPYQRKGFFPLMPGVFHAFYPNPYRTPWGVEPERVAEICLDFIEKTLFTTTTPADDVAAIIAEPIQGEGG
YVVPAPGFWQGLRDLCDRHGILLIADEVQSGVGRTGKMWAVEYEGIVPDIITSAKGIGSGVPVGAMVARKEISSVWKPGA
HGNTYGGNALAMRAVYETLCLAEEELMANANEVGGYFKQRLHELEDRYECIGDVRGRGLMIGMEFIKDNVNKDPHGDLSN
AVMEESFRRGMLLLTCGKSTIRFCPPLVLTKDQVDEGINLLNDTLQALGAK

Specific function: 4-aminobutyrate (GABA) degradation. [C]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=427, Percent_Identity=34.192037470726, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI4557809, Length=413, Percent_Identity=35.3510895883777, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI37574042, Length=425, Percent_Identity=29.8823529411765, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI24119277, Length=444, Percent_Identity=27.2522522522523, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI284507298, Length=328, Percent_Identity=35.6707317073171, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI226442709, Length=393, Percent_Identity=30.0254452926209, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI226442705, Length=426, Percent_Identity=29.5774647887324, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI188536080, Length=477, Percent_Identity=27.6729559748428, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI38679950, Length=477, Percent_Identity=27.6729559748428, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI38679946, Length=477, Percent_Identity=27.6729559748428, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1789016, Length=423, Percent_Identity=37.3522458628842, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI1787560, Length=405, Percent_Identity=38.7654320987654, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1788044, Length=404, Percent_Identity=37.3762376237624, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1789759, Length=403, Percent_Identity=34.9875930521092, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI145693181, Length=404, Percent_Identity=37.1287128712871, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1786991, Length=425, Percent_Identity=29.1764705882353, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1786349, Length=310, Percent_Identity=30.9677419354839, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32564660, Length=418, Percent_Identity=35.1674641148325, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI25144271, Length=413, Percent_Identity=32.9297820823245, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI71992977, Length=428, Percent_Identity=30.8411214953271, Blast_Score=184, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI17541228, Length=476, Percent_Identity=29.8319327731092, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25144274, Length=405, Percent_Identity=30.1234567901235, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71981843, Length=190, Percent_Identity=30.5263157894737, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324432, Length=436, Percent_Identity=31.8807339449541, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6323470, Length=419, Percent_Identity=31.2649164677804, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321456, Length=477, Percent_Identity=28.3018867924528, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324386, Length=373, Percent_Identity=25.4691689008043, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI161085790, Length=431, Percent_Identity=35.9628770301624, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI28574759, Length=426, Percent_Identity=36.150234741784, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI21357415, Length=430, Percent_Identity=32.5581395348837, Blast_Score=204, Evalue=8e-53,
Organism=Drosophila melanogaster, GI21356575, Length=428, Percent_Identity=32.0093457943925, Blast_Score=195, Evalue=6e-50,
Organism=Drosophila melanogaster, GI281366494, Length=476, Percent_Identity=26.6806722689076, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24667139, Length=476, Percent_Identity=26.6806722689076, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24667143, Length=476, Percent_Identity=26.6806722689076, Blast_Score=128, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.19

Molecular weight: Translated: 49552; Mature: 49421

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPINLFKEFPVTDLLTTIPGPRATALVDRDTAVMAPCSGRVYPFVMDRGLGCEVWDVDG
CCHHHHHHHCCHHHHHHHCCCCCEEEEEECCCEEECCCCCCEEEEEECCCCCCEEEECCC
NRYLDLNAGIAVVSTGHSHPRLVQALQDQVSKFIHMAGTDFYNEPMVKAAEKITSLMPGS
CEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
GEWQVFFANSGTESVEAAIKLARYYTKRQNVIGFYSSFHGRSYGSLSITASKPYQRKGFF
CCEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
PLMPGVFHAFYPNPYRTPWGVEPERVAEICLDFIEKTLFTTTTPADDVAAIIAEPIQGEG
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
GYVVPAPGFWQGLRDLCDRHGILLIADEVQSGVGRTGKMWAVEYEGIVPDIITSAKGIGS
CEEECCCHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCEEEEEECCCCHHHHHHCCCCCC
GVPVGAMVARKEISSVWKPGAHGNTYGGNALAMRAVYETLCLAEEELMANANEVGGYFKQ
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
RLHELEDRYECIGDVRGRGLMIGMEFIKDNVNKDPHGDLSNAVMEESFRRGMLLLTCGKS
HHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCC
TIRFCPPLVLTKDQVDEGINLLNDTLQALGAK
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPINLFKEFPVTDLLTTIPGPRATALVDRDTAVMAPCSGRVYPFVMDRGLGCEVWDVDG
CHHHHHHHCCHHHHHHHCCCCCEEEEEECCCEEECCCCCCEEEEEECCCCCCEEEECCC
NRYLDLNAGIAVVSTGHSHPRLVQALQDQVSKFIHMAGTDFYNEPMVKAAEKITSLMPGS
CEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
GEWQVFFANSGTESVEAAIKLARYYTKRQNVIGFYSSFHGRSYGSLSITASKPYQRKGFF
CCEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
PLMPGVFHAFYPNPYRTPWGVEPERVAEICLDFIEKTLFTTTTPADDVAAIIAEPIQGEG
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
GYVVPAPGFWQGLRDLCDRHGILLIADEVQSGVGRTGKMWAVEYEGIVPDIITSAKGIGS
CEEECCCHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCEEEEEECCCCHHHHHHCCCCCC
GVPVGAMVARKEISSVWKPGAHGNTYGGNALAMRAVYETLCLAEEELMANANEVGGYFKQ
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
RLHELEDRYECIGDVRGRGLMIGMEFIKDNVNKDPHGDLSNAVMEESFRRGMLLLTCGKS
HHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCC
TIRFCPPLVLTKDQVDEGINLLNDTLQALGAK
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate lysine-residue [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.3 {4-aminobutanoate}} 1.1 {4-aminobutanoate}} 1.3 {2-oxoglutarate}} 0.7 {2-oxoglutarate}} [C]

Substrates: 4-aminobutanoate; 2-oxoglutarate

Specific reaction: 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate

General reaction: Amino group transfer [C]

Inhibitor: 2-Oxoglutarate; Lysyl reagents; SH-group reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA