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The map label for this gene is tuf [H]

Identifier: 159896651

GI number: 159896651

Start: 152689

End: 153891

Strand: Direct

Name: tuf [H]

Synonym: Haur_0118

Alternate gene names: 159896651

Gene position: 152689-153891 (Clockwise)

Preceding gene: 159896650

Following gene: 159896652

Centisome position: 2.41

GC content: 55.69

Gene sequence:

>1203_bases
ATGGCTAAGCAAAAGTTCGAGCGGAACAAGCCCCACATCAACATTGGGACGATCGGGCACGTTGACCACGGCAAGACGAC
CTTGACCGCTGCCATCACCAAAACCATGGCACTGCGTGGCCGCGCTGAATTCCGCGCCTTCGACCAAATCGACAATGCTC
CCGAAGAACGCGCTCGTGGGATTACGATTTCGATTTCACACGTCGAGTATGAAACCGAAAATCGCCACTACGCCCACGTG
GACTGCCCCGGCCACGCCGACTATATCAAGAACATGATCACCGGTGCTGCCCAAATGGACGGCGCAATCTTGGTGGTGTC
AGCGCCCGACGGCCCGATGCCCCAAACCCGCGAACACATCCTCTTGGCTGGCCAAGTCGAAGTGCCTGCGATGGTGGTCT
TCTTGAACAAAGTTGACATGATGGACGACCCTGAGTTGCTCGAACTGGTTGAAATGGAATTGCGCGAGTTGCTGTCAAAA
TACGGCTTCCCGGGCGACGAAATTCCGATCGTGCGTGGCTCAGCCAAAGGCGCATTGGATAGCGCATCAACCGATGCAAG
CCAACCCGAGTATCAATCGATCCAAGAATTGATGCAAGCGGTTGACGATTATATCCCAACGCCAGAACGCGCCATCGACA
AACCATTCTTGATGCCGATTGAAGACGTGTTCTCGATCAAAGGCCGTGGCACCGTGGTGACCGGTCGGATCGAACGCGGG
ATCGTCAAGGTTGGCGATACGATTGAAATCATCGGGATGGGACCAGACGTGCGCACGACGGCAGTAACCGGCGTGGAAAT
GTTCAAGAAGTTGCTGGACGAAGGTCGCGCAGGCGACAACGTTGGGGCACTCTTGCGCGGGATTGAACGAACGGACGTGG
AACGCGGTCAAGTCTTGGCAAAACCAGGCTCGATCAAACCACACACCAAGTTCAAGGCCGAGGTCTACGTGTTGAAGAAA
GAAGAAGGTGGCCGCCACAGCCCATTCTTCAGTGGCTATCGCCCCCAATTCTACGTTCGGACGACGGACGTGACGGGTGC
GATTGGCTTGCCCGAAGGCGTGGAAATGGTGATGCCAGGCGACAACATCCAAATGACGGTGGAGTTGATCGTTCCGGTAG
CTATCGAACAAGGCTTGAAGTTTGCGATTCGTGAAGGTGGCCGCACCGTGGGTGCTGGCATCGTGACCGAAATTATCGCC
TAA

Upstream 100 bases:

>100_bases
GCTCCATACCTATGCTGGAAAACGATCCGACACTTTGTGCGGGTCGTTTTCATTGAAACTTACAAAACCCCATTTGGGAT
TGTAGGAGGACTAACACTCA

Downstream 100 bases:

>100_bases
ACTATTGCTTTGAATCAGCACAAACGCGATCAGCCATTTGGTCTGATCGCGTTTGTGCCTCAACCTGCGTTATCAGCAGG
CCAAGTATGACAAACGGGGG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAKQKFERNKPHINIGTIGHVDHGKTTLTAAITKTMALRGRAEFRAFDQIDNAPEERARGITISISHVEYETENRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHILLAGQVEVPAMVVFLNKVDMMDDPELLELVEMELRELLSK
YGFPGDEIPIVRGSAKGALDSASTDASQPEYQSIQELMQAVDDYIPTPERAIDKPFLMPIEDVFSIKGRGTVVTGRIERG
IVKVGDTIEIIGMGPDVRTTAVTGVEMFKKLLDEGRAGDNVGALLRGIERTDVERGQVLAKPGSIKPHTKFKAEVYVLKK
EEGGRHSPFFSGYRPQFYVRTTDVTGAIGLPEGVEMVMPGDNIQMTVELIVPVAIEQGLKFAIREGGRTVGAGIVTEIIA

Sequences:

>Translated_400_residues
MAKQKFERNKPHINIGTIGHVDHGKTTLTAAITKTMALRGRAEFRAFDQIDNAPEERARGITISISHVEYETENRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHILLAGQVEVPAMVVFLNKVDMMDDPELLELVEMELRELLSK
YGFPGDEIPIVRGSAKGALDSASTDASQPEYQSIQELMQAVDDYIPTPERAIDKPFLMPIEDVFSIKGRGTVVTGRIERG
IVKVGDTIEIIGMGPDVRTTAVTGVEMFKKLLDEGRAGDNVGALLRGIERTDVERGQVLAKPGSIKPHTKFKAEVYVLKK
EEGGRHSPFFSGYRPQFYVRTTDVTGAIGLPEGVEMVMPGDNIQMTVELIVPVAIEQGLKFAIREGGRTVGAGIVTEIIA
>Mature_399_residues
AKQKFERNKPHINIGTIGHVDHGKTTLTAAITKTMALRGRAEFRAFDQIDNAPEERARGITISISHVEYETENRHYAHVD
CPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHILLAGQVEVPAMVVFLNKVDMMDDPELLELVEMELRELLSKY
GFPGDEIPIVRGSAKGALDSASTDASQPEYQSIQELMQAVDDYIPTPERAIDKPFLMPIEDVFSIKGRGTVVTGRIERGI
VKVGDTIEIIGMGPDVRTTAVTGVEMFKKLLDEGRAGDNVGALLRGIERTDVERGQVLAKPGSIKPHTKFKAEVYVLKKE
EGGRHSPFFSGYRPQFYVRTTDVTGAIGLPEGVEMVMPGDNIQMTVELIVPVAIEQGLKFAIREGGRTVGAGIVTEIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=401, Percent_Identity=56.1097256857855, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=30.5803571428571, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=29.5454545454545, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=29.8206278026906, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=29.5454545454545, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI194018522, Length=378, Percent_Identity=29.1005291005291, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI194018520, Length=378, Percent_Identity=29.1005291005291, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194097354, Length=378, Percent_Identity=29.1005291005291, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI46094014, Length=328, Percent_Identity=31.0975609756098, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI54607086, Length=351, Percent_Identity=30.7692307692308, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI82546879, Length=433, Percent_Identity=24.9422632794457, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4503507, Length=272, Percent_Identity=28.3088235294118, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI45593140, Length=431, Percent_Identity=23.6658932714617, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157426893, Length=242, Percent_Identity=26.4462809917355, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI94966754, Length=142, Percent_Identity=32.3943661971831, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790412, Length=401, Percent_Identity=72.3192019950125, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI1789737, Length=401, Percent_Identity=72.3192019950125, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI2367247, Length=340, Percent_Identity=30, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789108, Length=436, Percent_Identity=25.2293577981651, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI48994988, Length=258, Percent_Identity=32.5581395348837, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788922, Length=333, Percent_Identity=25.8258258258258, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789559, Length=238, Percent_Identity=31.9327731092437, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=404, Percent_Identity=50, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141371, Length=391, Percent_Identity=43.4782608695652, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=30.8888888888889, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=30.8888888888889, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=443, Percent_Identity=28.8939051918736, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=28.7610619469027, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=442, Percent_Identity=26.6968325791855, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=442, Percent_Identity=26.6968325791855, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=277, Percent_Identity=29.9638989169675, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=38.7755102040816, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=278, Percent_Identity=27.3381294964029, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=274, Percent_Identity=28.8321167883212, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=400, Percent_Identity=64.5, Blast_Score=524, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=442, Percent_Identity=30.0904977375566, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319594, Length=442, Percent_Identity=30.0904977375566, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322937, Length=363, Percent_Identity=25.068870523416, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=29.2753623188406, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320863, Length=272, Percent_Identity=29.7794117647059, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=171, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=28.3950617283951, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=397, Percent_Identity=56.4231738035265, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=397, Percent_Identity=56.4231738035265, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=49.2307692307692, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=443, Percent_Identity=28.2167042889391, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45553816, Length=443, Percent_Identity=28.2167042889391, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24651721, Length=443, Percent_Identity=28.2167042889391, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17864154, Length=443, Percent_Identity=28.2167042889391, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24652838, Length=443, Percent_Identity=28.4424379232506, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17137572, Length=443, Percent_Identity=28.4424379232506, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI45550900, Length=448, Percent_Identity=27.9017857142857, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI19922690, Length=309, Percent_Identity=31.0679611650485, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=31.304347826087, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24647046, Length=284, Percent_Identity=26.7605633802817, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24647048, Length=284, Percent_Identity=26.7605633802817, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19922512, Length=429, Percent_Identity=24.7086247086247, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24654852, Length=429, Percent_Identity=24.7086247086247, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI78706572, Length=241, Percent_Identity=29.8755186721992, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28572034, Length=126, Percent_Identity=41.2698412698413, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43729; Mature: 43598

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKFERNKPHINIGTIGHVDHGKTTLTAAITKTMALRGRAEFRAFDQIDNAPEERARG
CCCCCHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCC
ITISISHVEYETENRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCCCEE
LLAGQVEVPAMVVFLNKVDMMDDPELLELVEMELRELLSKYGFPGDEIPIVRGSAKGALD
EEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCC
SASTDASQPEYQSIQELMQAVDDYIPTPERAIDKPFLMPIEDVFSIKGRGTVVTGRIERG
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEECCCC
IVKVGDTIEIIGMGPDVRTTAVTGVEMFKKLLDEGRAGDNVGALLRGIERTDVERGQVLA
EEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEE
KPGSIKPHTKFKAEVYVLKKEEGGRHSPFFSGYRPQFYVRTTDVTGAIGLPEGVEMVMPG
CCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECC
DNIQMTVELIVPVAIEQGLKFAIREGGRTVGAGIVTEIIA
CCCEEEEEEEEHHHHHHCHHHHHHCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKQKFERNKPHINIGTIGHVDHGKTTLTAAITKTMALRGRAEFRAFDQIDNAPEERARG
CCCCHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCC
ITISISHVEYETENRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCCCEE
LLAGQVEVPAMVVFLNKVDMMDDPELLELVEMELRELLSKYGFPGDEIPIVRGSAKGALD
EEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCC
SASTDASQPEYQSIQELMQAVDDYIPTPERAIDKPFLMPIEDVFSIKGRGTVVTGRIERG
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEECCCC
IVKVGDTIEIIGMGPDVRTTAVTGVEMFKKLLDEGRAGDNVGALLRGIERTDVERGQVLA
EEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEE
KPGSIKPHTKFKAEVYVLKKEEGGRHSPFFSGYRPQFYVRTTDVTGAIGLPEGVEMVMPG
CCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECC
DNIQMTVELIVPVAIEQGLKFAIREGGRTVGAGIVTEIIA
CCCEEEEEEEEHHHHHHCHHHHHHCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8085791 [H]