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The map label for this gene is dnaG

Identifier: 15927142

GI number: 15927142

Start: 1596711

End: 1598528

Strand: Reverse

Name: dnaG

Synonym: SA1391

Alternate gene names: 15927142

Gene position: 1598528-1596711 (Counterclockwise)

Preceding gene: 15927143

Following gene: 15927141

Centisome position: 56.79

GC content: 31.96

Gene sequence:

>1818_bases
TTGCGAATAGATCAATCGATCATTAATGAAATAAAAGATAAAACCGACATTTTAGACTTGGTAAGTGAATATGTAAAACT
AGAAAAGAGAGGACGCAATTATATAGGTTTGTGTCCTTTTCATGATGAAAAGACACCTTCATTTACAGTTTCTGAAGATA
AACAAATCTGTCATTGTTTTGGTTGTAAAAAAGGTGGCAATGTTTTTCAATTTACTCAAGAAATTAAAGACATATCATTT
GTTGAAGCGGTTAAAGAATTAGGTGATAGAGTTAATGTTGCTGTAGATATTGAGGCAACACAATCTAACTCAAATGTTCA
AATTGCTTCTGATGATTTACAAATGATTGAAATGCATGAGTTAATACAAGAATTTTATTATTACGCTTTAACAAAGACAG
TCGAAGGCGAACAAGCATTAACATACTTACAAGAACGTGGTTTTACAGATGCGCTTATTAAAGAGCGAGGCATTGGCTTT
GCACCCGATAGCTCACATTTTTGTCATGATTTTCTTCAAAAAAAGGGTTACGATATTGAATTAGCATATGAAGCCGGATT
ATTATCACGTAACGAAGAAAATTTCAGTTATTACGATAGATTTCGAAATCGTATTATGTTTCCTTTGAAAAATGCGCAAG
GAAGAATTGTTGGATATTCAGGTCGAACATATACCGGTCAAGAACCAAAATACCTAAATAGTCCTGAAACGCCTATCTTT
CAAAAAAGAAAGTTGTTATATAACTTAGATAAAGCACGTAAATCAATTAGAAAATTAGATGAAATTGTATTACTAGAAGG
TTTTATGGATGTTATAAAATCTGATACTGCTGGCTTGAAAAACGTTGTTGCAACAATGGGTACACAGTTGTCAGATGAAC
ATATTACCTTTATACGAAAGTTAACATCAAATATAACATTAATGTTTGATGGGGATTTTGCGGGTAGTGAAGCAACACTT
AAAACAGGTCAACATTTGTTACAGCAAGGGCTAAATGTATTTGTTATACAATTGCCATCTGGCATGGATCCGGATGAATA
CATTGGTAAGTATGGCAACGACGCATTTACTACTTTTGTAAAAAATGACAAAAAGTCATTTGCACATTATAAAGTAAGTA
TATTAAAAGATGAAATTGCACATAATGACCTTTCATATGAACGTTATTTGAAAGAACTGAGTCATGACATTTCACTTATG
AAGTCATCAATTCTGCAACAAAAGGCTATAAATGATGTTGCGCCATTTTTCAATGTTAGTCCTGAGCAGTTAGCTAACGA
AATACAATTCAATCAAGCACCAGCCAATTATTATCCAGAAGATGAGTATGGCGGTTATGATGAGTATGGCGGTTATATTG
AACCTGAGCCAATTGGTATGGCACAATTTGACAATTTGAGCCGTCAAGAAAAAGCGGAGCGAGCATTTTTAAAACATTTA
ATGAGAGATAAAGATACATTTTTAAATTATTATGAAAGTGTTGATAAGGATAACTTCACAAATCAGCATTTTAAATATGT
ATTCGAAGTCTTACATGATTTTTATGCGGAAAATGATCAATATAATATCAGTGATGCTGTGCAGTATGTTAATTCAAATG
AGTTGAGAGAAACACTAATTAGCTTAGAACAATATAATTTGAATGACGAACCATATGAAAATGAAATTGATGATTATGTC
AATGTTATTAATGAAAAAGGACAAGAAACAATTGAGTCATTGAATCATAAATTAAGGGAAGCTACAAGGATTGGCGATGT
AGAATTACAAAAATACTATTTACAGCAAATTGTTGCTAAGAATAAAGAACGCATGTAG

Upstream 100 bases:

>100_bases
CGATATAATCCATTATATTGAACAAAATAAATCGAAATGATTTCATTTTTGTCAAAAATTAGGTATAATAGTATAACTAA
TGCTTAATAGGTGATTTAAT

Downstream 100 bases:

>100_bases
CATGTGATTTTAAAGAATAATACGAATAATGATTATGTCAAAATGTATAAGGGTAAATGATAGTTACCGCATTTAAACAA
CACTATTGAAAAATAAATAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHL
MRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYV
NVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

Sequences:

>Translated_605_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHL
MRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYV
NVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM
>Mature_605_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHL
MRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYV
NVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=362, Percent_Identity=37.8453038674033, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_STAAM (P63964)

Other databases:

- EMBL:   BA000017
- RefSeq:   NP_372086.1
- ProteinModelPortal:   P63964
- STRING:   P63964
- EnsemblBacteria:   EBSTAT00000007942
- GeneID:   1121537
- GenomeReviews:   BA000017_GR
- KEGG:   sav:SAV1562
- eggNOG:   COG0358
- GeneTree:   EBGT00050000025353
- HOGENOM:   HBG573408
- OMA:   GFLWGGD
- ProtClustDB:   CLSK885432
- BioCyc:   SAUR158878:SAV1562-MONOMER
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:1.10.860.10
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF00772 DnaB; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2; SSF48024 DNA_helicase_DnaB-like_N

EC number: 2.7.7.-

Molecular weight: Translated: 70032; Mature: 70032

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHH
GCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHH
LIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEE
LAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
EEECCCCCCCCCCCHHHHHHHHHCEECEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHH
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
LTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFV
HHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCCHHHHHH
KNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKAINDVAPFFNVS
HCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHL
HHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHH
MRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHH
SLEQYNLNDEPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAK
HHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
NKERM
HCCCC
>Mature Secondary Structure
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHH
GCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHH
LIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEE
LAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
EEECCCCCCCCCCCHHHHHHHHHCEECEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHH
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
LTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFV
HHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCCHHHHHH
KNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKAINDVAPFFNVS
HCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHL
HHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHH
MRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHH
SLEQYNLNDEPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAK
HHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
NKERM
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146