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The map label for this gene is sigA

Identifier: 15927141

GI number: 15927141

Start: 1595381

End: 1596487

Strand: Reverse

Name: sigA

Synonym: SA1390

Alternate gene names: 15927141

Gene position: 1596487-1595381 (Counterclockwise)

Preceding gene: 15927142

Following gene: 15927140

Centisome position: 56.72

GC content: 33.79

Gene sequence:

>1107_bases
ATGTCTGATAACACAGTTAAAATTAAAAAACAAACAATTGATCCGACATTAACATTAGAAGATGTTAAGAAGCAATTAAT
TGAAAAAGGTAAAAAAGAGGGTCATTTAAGTCATGAAGAAATTGCTGAAAAACTTCAGAATTTTGATATCGACTCTGATC
AAATGGATGATTTCTTTGATCAATTAAATGATAATGATATTTCACTAGTTAATGAAAAAGATAGTTCAGATACTGACGAG
AAACTGAATCCAAGTGATCTTAGTGCCCCTCCAGGTGTTAAAATAAATGACCCAGTTCGTATGTACCTTAAAGAAATTGG
GCGTGTTAACTTATTAAGTGCACAAGAAGAAATCGAATTAGCCAAACGTATTGAACAAGGTGATGAAGTAGCAAAATCAA
GACTTGCAGAAGCGAACTTACGTTTAGTTGTAAGTATTGCTAAAAGATACGTAGGTCGTGGTATGTTATTCCTTGATTTA
ATCCAAGAAGGTAATATGGGTCTTATTAAAGCTGTTGAAAAATTTGACTTTAACAAAGGATTTAAGTTTTCAACATATGC
AACATGGTGGATTAGACAAGCAATCACTCGTGCAATTGCTGACCAAGCACGTACGATTCGTATCCCTGTGCATATGGTAG
AAACAATTAATAAATTAATTCGTGTTCAACGTCAATTATTACAGGACTTAGGTCGAGATCCAGCACCAGAAGAAATTGGT
GAAGAAATGGATTTACCAGCAGAAAAAGTTCGTGAAGTTTTAAAAATTGCGCAAGAACCTGTTTCATTAGAAACACCAAT
TGGTGAAGAAGATGATAGTCATTTAGGAGACTTTATTGAGGATCAGGAAGCACAAAGTCCTTCAGATCATGCTGCTTATG
AATTATTAAAAGAGCAATTAGAAGATGTGCTTGATACATTAACTGATAGAGAAGAAAATGTATTACGATTAAGATTTGGT
CTTGATGACGGCAGAACAAGAACACTTGAAGAAGTTGGTAAAGTTTTCGGTGTTACACGTGAACGTATTCGACAAATTGA
AGCAAAAGCACTTAGAAAATTAAGACATCCAAGTCGTAGTAAACGTTTGAAAGACTTTATGGATTAA

Upstream 100 bases:

>100_bases
ATAAAATTAAAAATATGGATGAATTAATTAAGAATTTAGTTTAAAATAGCAATATTGAATAAATTTCGAATGTTCATATT
TAAAATCGGGAGGCCGTTTC

Downstream 100 bases:

>100_bases
TTTATATCAATTTTAATGATTGATACCAAGACATGAATAAGCGCTTATTTATTTTTAGATTAAGATTTAACTTGAGTGAG
GTTGGTACATTGAATAATGT

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEKLQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDE
KLNPSDLSAPPGVKINDPVRMYLKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGRDPAPEEIG
EEMDLPAEKVREVLKIAQEPVSLETPIGEEDDSHLGDFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFG
LDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD

Sequences:

>Translated_368_residues
MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEKLQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDE
KLNPSDLSAPPGVKINDPVRMYLKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGRDPAPEEIG
EEMDLPAEKVREVLKIAQEPVSLETPIGEEDDSHLGDFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFG
LDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD
>Mature_367_residues
SDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEKLQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEK
LNPSDLSAPPGVKINDPVRMYLKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDLI
QEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGRDPAPEEIGE
EMDLPAEKVREVLKIAQEPVSLETPIGEEDDSHLGDFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGL
DDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This may be the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=67.6470588235294, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1789098, Length=303, Percent_Identity=41.9141914191419, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=29.7794117647059, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.7128712871287, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 42157; Mature: 42026

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEKLQNFDIDSDQMDDFFD
CCCCCEEEEEECCCCCEEHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCCHHHHHHHHH
QLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMYLKEIGRVNLLSAQEEIEL
HCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
AKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVEKFDFNKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCC
FKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGRDPAPEEIG
CCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EEMDLPAEKVREVLKIAQEPVSLETPIGEEDDSHLGDFIEDQEAQSPSDHAAYELLKEQL
HHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRS
HHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KRLKDFMD
HHHHHHCC
>Mature Secondary Structure 
SDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEKLQNFDIDSDQMDDFFD
CCCCEEEEEECCCCCEEHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCCHHHHHHHHH
QLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMYLKEIGRVNLLSAQEEIEL
HCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
AKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVEKFDFNKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCC
FKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGRDPAPEEIG
CCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EEMDLPAEKVREVLKIAQEPVSLETPIGEEDDSHLGDFIEDQEAQSPSDHAAYELLKEQL
HHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRS
HHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KRLKDFMD
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1923759 [H]