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The map label for this gene is aroE

Identifier: 15922604

GI number: 15922604

Start: 2281953

End: 2282735

Strand: Direct

Name: aroE

Synonym: ST2273

Alternate gene names: 15922604

Gene position: 2281953-2282735 (Clockwise)

Preceding gene: 15922603

Following gene: 15922605

Centisome position: 84.68

GC content: 29.63

Gene sequence:

>783_bases
ATGCTTGAGATAAATTATGATACGAAACTTTTAGGTGTAGTTGGCGAAAATATATCATATACATTATCACCAGCGATACA
CAATTATTCTTTTCAAGAATTAGGTATTAATGCTGTTTATTTAGCATTTGATATAAAAAGTGATGAATTTAAAGAAATAT
TCCCTGGGTTAGTCAAAATAGCATATGGTTTGAATATTACTATACCTTATAAGGAAATTGCAATAAAATATGTAGAAGCT
CAGAGTGAAGCAAAAAGAATTGGTGCAATAAATACTATTTTTAATGGCAAAGGATATAACACAGATTATATTGCAATTAA
GAGTTTGGTACAAGAGAGAATAGATAAATTTGAAACGTGTACTATATTCGGTGCTGGTGGGGCAGCTAGGGCAGCTATTT
TTGCTTTACATGATTTAGATTGCTCAATTAATGTTATAAATAGGAGTAAAGAGAAAGCTGAAAAATTAATAGAAGAAATG
AGGAATAAAAATATAGAAATAAAAATTACATATAATTGCAAAAGTGATATAATAGTTAATTCGACTCCTAATCCAGATTT
TGTTCCAGATGAGTGTGTTAATGGTAAATTAGTAATCGATTTTGTCTATAAACCAGTCATAACTTCATTGATCAAGAGGG
CTCAGAATAAGAATATAAAAACAATAAACGGTATCGAAATCCTTGTAAGGCAAGCAATGGAAGCTGAAAAAATTTGGTTT
GGAAAATCATTAGAAGATGAAGAAGTGGTGAATTATCTTTATGCCAGGAAACTCATTTGGTGA

Upstream 100 bases:

>100_bases
AAGTAAGAGGTGGATATGTAATGATGCCTTTTCCAACTAGGATAGGTGATTGGAAAAGAGTAGATGTACCGATAGAAACT
TTAAAGGGGTTTGCAGAGCA

Downstream 100 bases:

>100_bases
ATTATTTAGAATAACAACTTTCGGAGAGAGTCATGGTCCTATGGTTGGTGTCGTGATAGATGGAGTACCAGCAGGATTAC
CAATAAAAAAAGAAGACATA

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLEINYDTKLLGVVGENISYTLSPAIHNYSFQELGINAVYLAFDIKSDEFKEIFPGLVKIAYGLNITIPYKEIAIKYVEA
QSEAKRIGAINTIFNGKGYNTDYIAIKSLVQERIDKFETCTIFGAGGAARAAIFALHDLDCSINVINRSKEKAEKLIEEM
RNKNIEIKITYNCKSDIIVNSTPNPDFVPDECVNGKLVIDFVYKPVITSLIKRAQNKNIKTINGIEILVRQAMEAEKIWF
GKSLEDEEVVNYLYARKLIW

Sequences:

>Translated_260_residues
MLEINYDTKLLGVVGENISYTLSPAIHNYSFQELGINAVYLAFDIKSDEFKEIFPGLVKIAYGLNITIPYKEIAIKYVEA
QSEAKRIGAINTIFNGKGYNTDYIAIKSLVQERIDKFETCTIFGAGGAARAAIFALHDLDCSINVINRSKEKAEKLIEEM
RNKNIEIKITYNCKSDIIVNSTPNPDFVPDECVNGKLVIDFVYKPVITSLIKRAQNKNIKTINGIEILVRQAMEAEKIWF
GKSLEDEEVVNYLYARKLIW
>Mature_260_residues
MLEINYDTKLLGVVGENISYTLSPAIHNYSFQELGINAVYLAFDIKSDEFKEIFPGLVKIAYGLNITIPYKEIAIKYVEA
QSEAKRIGAINTIFNGKGYNTDYIAIKSLVQERIDKFETCTIFGAGGAARAAIFALHDLDCSINVINRSKEKAEKLIEEM
RNKNIEIKITYNCKSDIIVNSTPNPDFVPDECVNGKLVIDFVYKPVITSLIKRAQNKNIKTINGIEILVRQAMEAEKIWF
GKSLEDEEVVNYLYARKLIW

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=284, Percent_Identity=30.2816901408451, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789675, Length=262, Percent_Identity=26.3358778625954, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320332, Length=269, Percent_Identity=28.996282527881, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_SULTO (Q96Y95)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_378273.1
- HSSP:   Q58484
- ProteinModelPortal:   Q96Y95
- SMR:   Q96Y95
- GeneID:   1460355
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST2273
- NMPDR:   fig|273063.1.peg.2441
- HOGENOM:   HBG553408
- OMA:   GYNTDAS
- ProtClustDB:   CLSK802661
- BioCyc:   STOK273063:ST2273-MONOMER
- BRENDA:   1.1.1.25
- GO:   GO:0005737
- GO:   GO:0005488
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 29435; Mature: 29435

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: ACT_SITE 71-71

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEINYDTKLLGVVGENISYTLSPAIHNYSFQELGINAVYLAFDIKSDEFKEIFPGLVKI
CEEECCCCEEEEEECCCCEEEECHHHCCCCHHHCCCEEEEEEEECCCCHHHHHHHHHHHH
AYGLNITIPYKEIAIKYVEAQSEAKRIGAINTIFNGKGYNTDYIAIKSLVQERIDKFETC
HHCCEEEECHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEE
TIFGAGGAARAAIFALHDLDCSINVINRSKEKAEKLIEEMRNKNIEIKITYNCKSDIIVN
EEEECCCHHHHEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEE
STPNPDFVPDECVNGKLVIDFVYKPVITSLIKRAQNKNIKTINGIEILVRQAMEAEKIWF
CCCCCCCCCCHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCC
GKSLEDEEVVNYLYARKLIW
CCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLEINYDTKLLGVVGENISYTLSPAIHNYSFQELGINAVYLAFDIKSDEFKEIFPGLVKI
CEEECCCCEEEEEECCCCEEEECHHHCCCCHHHCCCEEEEEEEECCCCHHHHHHHHHHHH
AYGLNITIPYKEIAIKYVEAQSEAKRIGAINTIFNGKGYNTDYIAIKSLVQERIDKFETC
HHCCEEEECHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEE
TIFGAGGAARAAIFALHDLDCSINVINRSKEKAEKLIEEMRNKNIEIKITYNCKSDIIVN
EEEECCCHHHHEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEE
STPNPDFVPDECVNGKLVIDFVYKPVITSLIKRAQNKNIKTINGIEILVRQAMEAEKIWF
CCCCCCCCCCHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCC
GKSLEDEEVVNYLYARKLIW
CCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479