Click here to switch to the map view.

The map label for this gene is prpD1 [H]

Identifier: 15922123

GI number: 15922123

Start: 1825856

End: 1827151

Strand: Reverse

Name: prpD1 [H]

Synonym: ST1810

Alternate gene names: 15922123

Gene position: 1827151-1825856 (Counterclockwise)

Preceding gene: 15922128

Following gene: 15922122

Centisome position: 67.8

GC content: 36.03

Gene sequence:

>1296_bases
ATGGAACTTTCAGATAAAATAGCCGAATACGTGGTCTCAGCTGAGGATTTTTCAGATGAAATGCTTCATGAGGCTAAAAG
AAGATTACTAGATAGCATAGCTGTAGCTCTAGCATCAATTAACTCACCACCTGCAAAAATTTTAAGAGAAATGTCAAATC
TCTTCGCTGGAAATTCTCCTCTTTTAAGTGGAGGAGAAGCCACTATAGATTTTGCATCATTTTATAATACCTTACTTATA
CGTTATTTAGACTTTAATGACACGTATTTGAGTAAAGAGCCTTTACATCCAAGTGATATGATAGGAGGTCTATTATCTGT
AGGTAGCGTGTTTGATATCTCTGGTGAAGAACTATTAAAGAGTATCATCGTGGGTTATGAAATTGGTGTGAGGCTCTGTG
ATAGTACAAGTCTAAGAAAAAAGGGATATGATCACGTTAATTTCCTTCAAATAGCTTCTACTTCAGCACTTTCTCGCCTC
CTTAACCTTGATAGAGAGAAGGCTAAGAATGCAATATCTTTAACGCTTGTTCCTCATATTGCGCTTAGGGAATCAAGATC
TGGTAAGTTATCAATGTGGAAAGCTGGAGCTACGGCTGAGGCTGTAAGGAATTCAGTATTTGCGACTCTTTTAGCTAAAA
ACGGATTTACTGCACCAGACAAACCGTTTTCTGGAGTTTTTGGATTCTTTAATATCATAGCTAAGGATTTTGATTTATCA
ACTTTCGAAAATATTAAGAGTGGGTCTATTCTTAAGACTTTCATTAAAAAATATCCAGTTGAATACCATGCTGAAGCCAC
TGTCGAAGCGGCATTGAAACTAGACTATGAAGGTGAAATAAAGAAAGTAATTGTAGAAACTTACGAAGCTGGTAAGACAA
TATTGGCTGATAGTGAGGATAAATGGAATCCTAAAAATAAAGAAACTGCCGATCATAGTTTACCATTTATTACGGCAGTT
ACTTTACTTACAAAGCGTTTCTGGTTGGATTCGTATAGCTTAATTGGAGACCCTAAAGTAGTTAGCTTAATGAAAAAGAT
TGAGGTCGTAGAGAGGGATGATTATACTTCTGTTTATCCTAAAGAACTACCTACTAGAGTTATTGTCATTACAGATAAGG
GTACTTATGAAAGTGAAGTAAGAGTTCCTAGAGGGCATTCAGCAAATCCTATGAGTGATGAGGAAATTGAAGAAAAAGCT
AAGCTTTTAGGGCTTTCAGAAAGTCAAATTAAGCTCATTTGGGAAATAGATAATATGAAGGTGAGGGATTTTGTCAGAAA
TCTTGCGAAAGTCTGA

Upstream 100 bases:

>100_bases
TAAAAGCTATCTTTGTCATGTTTATCATGTTTAAAATTCTCTATTTGATTATTTAAATTTATCTCAAAAAGTTTATATTG
GTAGTTTCAAAGAGAAAGTT

Downstream 100 bases:

>100_bases
TTTCCTAATTATTCCGGGCGTATTTAATCCTTTTACAGCATTACTAGCTGAAAAAGTAGGATTTAAAGCAGTGTATCTGT
CTGGAGGAGCTTTAACGTCC

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MELSDKIAEYVVSAEDFSDEMLHEAKRRLLDSIAVALASINSPPAKILREMSNLFAGNSPLLSGGEATIDFASFYNTLLI
RYLDFNDTYLSKEPLHPSDMIGGLLSVGSVFDISGEELLKSIIVGYEIGVRLCDSTSLRKKGYDHVNFLQIASTSALSRL
LNLDREKAKNAISLTLVPHIALRESRSGKLSMWKAGATAEAVRNSVFATLLAKNGFTAPDKPFSGVFGFFNIIAKDFDLS
TFENIKSGSILKTFIKKYPVEYHAEATVEAALKLDYEGEIKKVIVETYEAGKTILADSEDKWNPKNKETADHSLPFITAV
TLLTKRFWLDSYSLIGDPKVVSLMKKIEVVERDDYTSVYPKELPTRVIVITDKGTYESEVRVPRGHSANPMSDEEIEEKA
KLLGLSESQIKLIWEIDNMKVRDFVRNLAKV

Sequences:

>Translated_431_residues
MELSDKIAEYVVSAEDFSDEMLHEAKRRLLDSIAVALASINSPPAKILREMSNLFAGNSPLLSGGEATIDFASFYNTLLI
RYLDFNDTYLSKEPLHPSDMIGGLLSVGSVFDISGEELLKSIIVGYEIGVRLCDSTSLRKKGYDHVNFLQIASTSALSRL
LNLDREKAKNAISLTLVPHIALRESRSGKLSMWKAGATAEAVRNSVFATLLAKNGFTAPDKPFSGVFGFFNIIAKDFDLS
TFENIKSGSILKTFIKKYPVEYHAEATVEAALKLDYEGEIKKVIVETYEAGKTILADSEDKWNPKNKETADHSLPFITAV
TLLTKRFWLDSYSLIGDPKVVSLMKKIEVVERDDYTSVYPKELPTRVIVITDKGTYESEVRVPRGHSANPMSDEEIEEKA
KLLGLSESQIKLIWEIDNMKVRDFVRNLAKV
>Mature_431_residues
MELSDKIAEYVVSAEDFSDEMLHEAKRRLLDSIAVALASINSPPAKILREMSNLFAGNSPLLSGGEATIDFASFYNTLLI
RYLDFNDTYLSKEPLHPSDMIGGLLSVGSVFDISGEELLKSIIVGYEIGVRLCDSTSLRKKGYDHVNFLQIASTSALSRL
LNLDREKAKNAISLTLVPHIALRESRSGKLSMWKAGATAEAVRNSVFATLLAKNGFTAPDKPFSGVFGFFNIIAKDFDLS
TFENIKSGSILKTFIKKYPVEYHAEATVEAALKLDYEGEIKKVIVETYEAGKTILADSEDKWNPKNKETADHSLPFITAV
TLLTKRFWLDSYSLIGDPKVVSLMKKIEVVERDDYTSVYPKELPTRVIVITDKGTYESEVRVPRGHSANPMSDEEIEEKA
KLLGLSESQIKLIWEIDNMKVRDFVRNLAKV

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=423, Percent_Identity=29.0780141843972, Blast_Score=133, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325258, Length=425, Percent_Identity=27.2941176470588, Blast_Score=114, Evalue=4e-26,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 48040; Mature: 48040

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSDKIAEYVVSAEDFSDEMLHEAKRRLLDSIAVALASINSPPAKILREMSNLFAGNSP
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
LLSGGEATIDFASFYNTLLIRYLDFNDTYLSKEPLHPSDMIGGLLSVGSVFDISGEELLK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCEECCCHHHHHH
SIIVGYEIGVRLCDSTSLRKKGYDHVNFLQIASTSALSRLLNLDREKAKNAISLTLVPHI
HHHHHHHHCEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCEEEEEEECHH
ALRESRSGKLSMWKAGATAEAVRNSVFATLLAKNGFTAPDKPFSGVFGFFNIIAKDFDLS
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCH
TFENIKSGSILKTFIKKYPVEYHAEATVEAALKLDYEGEIKKVIVETYEAGKTILADSED
HHHHCCCCHHHHHHHHHCCCCCCCHHHEEHHEEECCCCHHHHHHHHHHHCCCEEEECCCC
KWNPKNKETADHSLPFITAVTLLTKRFWLDSYSLIGDPKVVSLMKKIEVVERDDYTSVYP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCC
KELPTRVIVITDKGTYESEVRVPRGHSANPMSDEEIEEKAKLLGLSESQIKLIWEIDNMK
HHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCC
VRDFVRNLAKV
HHHHHHHHHCC
>Mature Secondary Structure
MELSDKIAEYVVSAEDFSDEMLHEAKRRLLDSIAVALASINSPPAKILREMSNLFAGNSP
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
LLSGGEATIDFASFYNTLLIRYLDFNDTYLSKEPLHPSDMIGGLLSVGSVFDISGEELLK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCEECCCHHHHHH
SIIVGYEIGVRLCDSTSLRKKGYDHVNFLQIASTSALSRLLNLDREKAKNAISLTLVPHI
HHHHHHHHCEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCEEEEEEECHH
ALRESRSGKLSMWKAGATAEAVRNSVFATLLAKNGFTAPDKPFSGVFGFFNIIAKDFDLS
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCH
TFENIKSGSILKTFIKKYPVEYHAEATVEAALKLDYEGEIKKVIVETYEAGKTILADSED
HHHHCCCCHHHHHHHHHCCCCCCCHHHEEHHEEECCCCHHHHHHHHHHHCCCEEEECCCC
KWNPKNKETADHSLPFITAVTLLTKRFWLDSYSLIGDPKVVSLMKKIEVVERDDYTSVYP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCC
KELPTRVIVITDKGTYESEVRVPRGHSANPMSDEEIEEKAKLLGLSESQIKLIWEIDNMK
HHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCC
VRDFVRNLAKV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626 [H]