Click here to switch to the map view.

The map label for this gene is prpB [H]

Identifier: 15922122

GI number: 15922122

Start: 1825039

End: 1825785

Strand: Reverse

Name: prpB [H]

Synonym: ST1808

Alternate gene names: 15922122

Gene position: 1825785-1825039 (Counterclockwise)

Preceding gene: 15922123

Following gene: 15922120

Centisome position: 67.75

GC content: 35.88

Gene sequence:

>747_bases
GTGTATCTGTCTGGAGGAGCTTTAACGTCCTCATACGGCTTACCAGATCTTGGCATAATAACGTTAGATGAAGTAGCAGA
AATGGTCAGAAGAATTAGGGAAGTCACTGATATTCCAATTATTGTTGATGCTGATACTGGCTTTGGAGAAGTTATAAATG
TATATAGGGCTGTGAAAGTTTTGGAAAAAGCTGGTGCCAATGCTATTCAAATTGAGGATCAAGTTCTTCCAAAGAAATGT
GGTCATCTAGAAGGTAAAGAAGTTGTGAGTTCTAAAGATATGGTTGCTAAAATAAAATCAGCACTAAAGGCCAGAAAAGA
TATGTTAATAATTGCTAGAACTGACGCAAGAGCGGTAAATGGTCTTGAAGACGCTATTGAGAGAGCTAAGATGTATTTAG
AAGCTGGTGCTGATATAATTTTTCCAGAAGCATTGGAAAGCAAAGAAGAATTCGCCAAATTTGCTAAAGAAGTTAAAGCA
CCACTATTGGCTAATATGACAGAGTTTGGTAAAACGCCGTTAATAACTGCTAATGAGTTTAAAGAAATGGGGTATAAGTA
CGTTATCTTTCCAGTGACTATTTTCAGAGTTGCAGCAAAAGCCATGAAAGAAGCGTTAGAGGTACTTCTTAAAGAAGGAT
CTCAAAAATCATTGATGGATAAGATGATGACTAGGAAGGAGCAGTATGAAATTATCAATTATTACTTTTATGAGAATTTA
GATAAGCAACTTGCGAAAGATTTATAG

Upstream 100 bases:

>100_bases
GATTTTGTCAGAAATCTTGCGAAAGTCTGATTTCCTAATTATTCCGGGCGTATTTAATCCTTTTACAGCATTACTAGCTG
AAAAAGTAGGATTTAAAGCA

Downstream 100 bases:

>100_bases
TTTACGAAAGAGATATAAAAATTATGACGCCGAATAAAATCAAATTACTAATAAATAAACCGATAATTAAGGTACAAAAA
GGGACTAGTGCTAGGGATGC

Product: carboxyvinyl-carboxyphosphonate phosphorylmutase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MYLSGGALTSSYGLPDLGIITLDEVAEMVRRIREVTDIPIIVDADTGFGEVINVYRAVKVLEKAGANAIQIEDQVLPKKC
GHLEGKEVVSSKDMVAKIKSALKARKDMLIIARTDARAVNGLEDAIERAKMYLEAGADIIFPEALESKEEFAKFAKEVKA
PLLANMTEFGKTPLITANEFKEMGYKYVIFPVTIFRVAAKAMKEALEVLLKEGSQKSLMDKMMTRKEQYEIINYYFYENL
DKQLAKDL

Sequences:

>Translated_248_residues
MYLSGGALTSSYGLPDLGIITLDEVAEMVRRIREVTDIPIIVDADTGFGEVINVYRAVKVLEKAGANAIQIEDQVLPKKC
GHLEGKEVVSSKDMVAKIKSALKARKDMLIIARTDARAVNGLEDAIERAKMYLEAGADIIFPEALESKEEFAKFAKEVKA
PLLANMTEFGKTPLITANEFKEMGYKYVIFPVTIFRVAAKAMKEALEVLLKEGSQKSLMDKMMTRKEQYEIINYYFYENL
DKQLAKDL
>Mature_248_residues
MYLSGGALTSSYGLPDLGIITLDEVAEMVRRIREVTDIPIIVDADTGFGEVINVYRAVKVLEKAGANAIQIEDQVLPKKC
GHLEGKEVVSSKDMVAKIKSALKARKDMLIIARTDARAVNGLEDAIERAKMYLEAGADIIFPEALESKEEFAKFAKEVKA
PLLANMTEFGKTPLITANEFKEMGYKYVIFPVTIFRVAAKAMKEALEVLLKEGSQKSLMDKMMTRKEQYEIINYYFYENL
DKQLAKDL

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=251, Percent_Identity=49.003984063745, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1790445, Length=197, Percent_Identity=27.4111675126904, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 27745; Mature: 27745

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLSGGALTSSYGLPDLGIITLDEVAEMVRRIREVTDIPIIVDADTGFGEVINVYRAVKV
CCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
LEKAGANAIQIEDQVLPKKCGHLEGKEVVSSKDMVAKIKSALKARKDMLIIARTDARAVN
HHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHH
GLEDAIERAKMYLEAGADIIFPEALESKEEFAKFAKEVKAPLLANMTEFGKTPLITANEF
HHHHHHHHHHHHHHHCCCEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
KEMGYKYVIFPVTIFRVAAKAMKEALEVLLKEGSQKSLMDKMMTRKEQYEIINYYFYENL
HHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DKQLAKDL
HHHHHHCC
>Mature Secondary Structure
MYLSGGALTSSYGLPDLGIITLDEVAEMVRRIREVTDIPIIVDADTGFGEVINVYRAVKV
CCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
LEKAGANAIQIEDQVLPKKCGHLEGKEVVSSKDMVAKIKSALKARKDMLIIARTDARAVN
HHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHH
GLEDAIERAKMYLEAGADIIFPEALESKEEFAKFAKEVKAPLLANMTEFGKTPLITANEF
HHHHHHHHHHHHHHHCCCEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
KEMGYKYVIFPVTIFRVAAKAMKEALEVLLKEGSQKSLMDKMMTRKEQYEIINYYFYENL
HHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DKQLAKDL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]