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The map label for this gene is carA

Identifier: 15921797

GI number: 15921797

Start: 1499780

End: 1500898

Strand: Direct

Name: carA

Synonym: ST1503

Alternate gene names: 15921797

Gene position: 1499780-1500898 (Clockwise)

Preceding gene: 15921796

Following gene: 15921798

Centisome position: 55.66

GC content: 35.39

Gene sequence:

>1119_bases
ATGACCTTATATTGTAAGAGAGGATATAAAGGGTATTTATACCTAGAAGATGGTACATTAATTGAAGGTTGCGGTTTTGG
AGCTAAAGGAATTAGAGCTGGAGAAGTAGTTTTCACTACATCAATGAATGGCTATCCAGAAAGCTTAACCGATCCTTCAT
ATAGAGGGCAAATCCTTGTAATAACACATCCACTGGTAGGAAATTACGGAGTCCCAGAAAAGCAAAGAGTAGAGGGAATA
TTAACTAACTTCGAGTCAGAGCAAATACAAGTTGAGGGTCTTGTAGTATCTGAGGAAACAGATCCCTTTAAATGGAATTC
GTCTAAAAGCCTTCATGAATGGTTACTAAGTGAGGGGGTTCCAGGGCTTTCAGACGTTGATACTAGAAGTATCGTTAAAA
AAGTAAGAAGTAGAGGAGTCATGATGGGTGTAATAGCATCTGGTTATGAGATAGAAGATCCAAAAAAGTTCCTAGAAAAG
AAGTATGATGAGATTGATTTTACGCAGTTTACATCACCAAAAGCTCCCATAGTTCATTTAGGTAATACCGGTGAAACAAT
AGTAGTGGTAGATTGTGGAGTAAAGCATGGTATACTATATCAACTTCATCAAAGAGGATTCACAATTGTAAGAGTACCTT
GTAAGTTTAATGTAAGTAAAATAATGGATTATTATCCTAAAGGTGTAGTTTTTGGCAATGGGCCTGGTAATCCAAACTTA
TTGCAAGAAGTTATAGAGAATTTTAAAGAATTAACAGAATATAAAATCCCAATTTTAGGAATTTGCTTAGGTCATCAAGT
AGCAACTTTAGCAATGGGGGGGAAGGTAAATAAAATGAAATTTGGACATAGAGCAATAAACAAACCAGTTATTGATATCT
CATCAAATAAATGTTATATTACAACGCATAATCATGGTTATGGTATTCTTTCAAAAGAAGATCTACCACCAAATAGTAAG
TTGTGGTTTATTAACCCAGATGACGGAACAATTGAAGGTTGGATTCATGAAAAGTTACCAATTATTACTACTCAGTTTCA
TCCTGAAGCTAGACCTGGTCCTTGGGATGTGACCTGGGTATTTGATAAATTTAAAAAGATGGTGAGTCGTAATGCGTGA

Upstream 100 bases:

>100_bases
ATAAAAATTAAAGAAAATAGAATAGCAGAAGAAGAGAGTAAATTGAAAAAATATGAAGAAAAAATAATCGGAAAAATGAA
TGAATTAAAGGTGATCGAAG

Downstream 100 bases:

>100_bases
GAGTGTAAGAAAAGTATTAGTTATTGGGTCTGGACCAATTAAAATAGCGGAAGCAGCAGAGTTTGATTACTCTGGAAGTC
AAGCATTAAAGGCATTAAAA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTLYCKRGYKGYLYLEDGTLIEGCGFGAKGIRAGEVVFTTSMNGYPESLTDPSYRGQILVITHPLVGNYGVPEKQRVEGI
LTNFESEQIQVEGLVVSEETDPFKWNSSKSLHEWLLSEGVPGLSDVDTRSIVKKVRSRGVMMGVIASGYEIEDPKKFLEK
KYDEIDFTQFTSPKAPIVHLGNTGETIVVVDCGVKHGILYQLHQRGFTIVRVPCKFNVSKIMDYYPKGVVFGNGPGNPNL
LQEVIENFKELTEYKIPILGICLGHQVATLAMGGKVNKMKFGHRAINKPVIDISSNKCYITTHNHGYGILSKEDLPPNSK
LWFINPDDGTIEGWIHEKLPIITTQFHPEARPGPWDVTWVFDKFKKMVSRNA

Sequences:

>Translated_372_residues
MTLYCKRGYKGYLYLEDGTLIEGCGFGAKGIRAGEVVFTTSMNGYPESLTDPSYRGQILVITHPLVGNYGVPEKQRVEGI
LTNFESEQIQVEGLVVSEETDPFKWNSSKSLHEWLLSEGVPGLSDVDTRSIVKKVRSRGVMMGVIASGYEIEDPKKFLEK
KYDEIDFTQFTSPKAPIVHLGNTGETIVVVDCGVKHGILYQLHQRGFTIVRVPCKFNVSKIMDYYPKGVVFGNGPGNPNL
LQEVIENFKELTEYKIPILGICLGHQVATLAMGGKVNKMKFGHRAINKPVIDISSNKCYITTHNHGYGILSKEDLPPNSK
LWFINPDDGTIEGWIHEKLPIITTQFHPEARPGPWDVTWVFDKFKKMVSRNA
>Mature_371_residues
TLYCKRGYKGYLYLEDGTLIEGCGFGAKGIRAGEVVFTTSMNGYPESLTDPSYRGQILVITHPLVGNYGVPEKQRVEGIL
TNFESEQIQVEGLVVSEETDPFKWNSSKSLHEWLLSEGVPGLSDVDTRSIVKKVRSRGVMMGVIASGYEIEDPKKFLEKK
YDEIDFTQFTSPKAPIVHLGNTGETIVVVDCGVKHGILYQLHQRGFTIVRVPCKFNVSKIMDYYPKGVVFGNGPGNPNLL
QEVIENFKELTEYKIPILGICLGHQVATLAMGGKVNKMKFGHRAINKPVIDISSNKCYITTHNHGYGILSKEDLPPNSKL
WFINPDDGTIEGWIHEKLPIITTQFHPEARPGPWDVTWVFDKFKKMVSRNA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=360, Percent_Identity=40, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI169790915, Length=376, Percent_Identity=36.1702127659575, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI21361331, Length=376, Percent_Identity=36.1702127659575, Blast_Score=243, Evalue=2e-64,
Organism=Escherichia coli, GI1786215, Length=382, Percent_Identity=33.7696335078534, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI193204318, Length=367, Percent_Identity=37.6021798365123, Blast_Score=253, Evalue=8e-68,
Organism=Saccharomyces cerevisiae, GI6324878, Length=365, Percent_Identity=41.6438356164384, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6322331, Length=388, Percent_Identity=38.1443298969072, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI45555749, Length=378, Percent_Identity=38.3597883597884, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24642586, Length=378, Percent_Identity=38.3597883597884, Blast_Score=235, Evalue=3e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_SULTO (Q970U8)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377466.1
- ProteinModelPortal:   Q970U8
- SMR:   Q970U8
- GeneID:   1459539
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1503
- NMPDR:   fig|273063.1.peg.1634
- HOGENOM:   HBG286341
- OMA:   DTEKLRF
- ProtClustDB:   PRK12564
- BioCyc:   STOK273063:ST1503-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_A
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 41673; Mature: 41542

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS51273 GATASE_TYPE_1; PS00888 CNMP_BINDING_1 ; PS00442 GATASE_TYPE_I ; PS00178 AA_TRNA_LIGASE_I

Important sites: ACT_SITE 262-262 ACT_SITE 347-347 ACT_SITE 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLYCKRGYKGYLYLEDGTLIEGCGFGAKGIRAGEVVFTTSMNGYPESLTDPSYRGQILV
CEEEEECCCEEEEEEECCCEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEE
ITHPLVGNYGVPEKQRVEGILTNFESEQIQVEGLVVSEETDPFKWNSSKSLHEWLLSEGV
EECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHCCC
PGLSDVDTRSIVKKVRSRGVMMGVIASGYEIEDPKKFLEKKYDEIDFTQFTSPKAPIVHL
CCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHCCCCCCEECCCCCCCEEEE
GNTGETIVVVDCGVKHGILYQLHQRGFTIVRVPCKFNVSKIMDYYPKGVVFGNGPGNPNL
CCCCCEEEEEECCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCEEECCCCCCHHH
LQEVIENFKELTEYKIPILGICLGHQVATLAMGGKVNKMKFGHRAINKPVIDISSNKCYI
HHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCEEECCHHHHCCCEEEECCCEEEE
TTHNHGYGILSKEDLPPNSKLWFINPDDGTIEGWIHEKLPIITTQFHPEARPGPWDVTWV
EECCCCEEEEECCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEHH
FDKFKKMVSRNA
HHHHHHHHHCCC
>Mature Secondary Structure 
TLYCKRGYKGYLYLEDGTLIEGCGFGAKGIRAGEVVFTTSMNGYPESLTDPSYRGQILV
EEEEECCCEEEEEEECCCEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEE
ITHPLVGNYGVPEKQRVEGILTNFESEQIQVEGLVVSEETDPFKWNSSKSLHEWLLSEGV
EECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHCCC
PGLSDVDTRSIVKKVRSRGVMMGVIASGYEIEDPKKFLEKKYDEIDFTQFTSPKAPIVHL
CCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHCCCCCCEECCCCCCCEEEE
GNTGETIVVVDCGVKHGILYQLHQRGFTIVRVPCKFNVSKIMDYYPKGVVFGNGPGNPNL
CCCCCEEEEEECCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCEEECCCCCCHHH
LQEVIENFKELTEYKIPILGICLGHQVATLAMGGKVNKMKFGHRAINKPVIDISSNKCYI
HHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCEEECCHHHHCCCEEEECCCEEEE
TTHNHGYGILSKEDLPPNSKLWFINPDDGTIEGWIHEKLPIITTQFHPEARPGPWDVTWV
EECCCCEEEEECCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEHH
FDKFKKMVSRNA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479