Click here to switch to the map view.

The map label for this gene is pyrE

Identifier: 15921774

GI number: 15921774

Start: 1479678

End: 1480265

Strand: Direct

Name: pyrE

Synonym: ST1481

Alternate gene names: 15921774

Gene position: 1479678-1480265 (Clockwise)

Preceding gene: 15921763

Following gene: 15921775

Centisome position: 54.91

GC content: 31.63

Gene sequence:

>588_bases
ATGGATTTTATTAAATCCTTAGTTGAGAATAAGCTTCTCTTGATAGGTAATTTCGTTTTAACATCTGGGAAGATAAGCCC
ATATTATCTAGATTTAAGAAGATTATCTAATTACTATGAAATTTTTTCTGATACTGTAAATAAGGCAATAGATAAGATAA
AAAATATAGATTTTGATATGATCATAGGAATAGCAACTGGAGGAATTCCTTTTGCATCATTCATATCTTGCAGAATGGGA
AAGCCTTTAGGATATATAAGAATGGAAAAGAAAGGGTATGGTACAGATAAGATCCTTGAGGCAGATGTAAGCGGAAGGAA
GATACTCTTAGTTGATGATGTTGCTACTACAGGTGGATCCCTAAGTAAGGCCGTTGAGATAATTAACTCTGAAGGCGGAA
GGGTTGTAGCAAGTTTAGTTATAGTTGATAGAGAAGAAGGTGCAGAGAAAAAACTAGGTGAGTATGGAGTCAAACTAATT
TCTGTATATAAAATTAGGGAGATTTTGGAGTATTTGCTAAATTCTAGTTTAATTTCGGATAACGATAAAAATAATATAAA
AGAATATTTGGTGAAAAATATTGAATAG

Upstream 100 bases:

>100_bases
CTTTTTGAAAAATCATATACTGATATTATATCCTTCACAAATGCCCTTTATTTATCTTCGTTCAATGATTATTTAAACTC
TTTCTTTCATAATCTAATTC

Downstream 100 bases:

>100_bases
AGTGATTCTTTCGTTAGATAGACAAATACCTCTTTCTACTCTATCAGAAATAAATAAATATATACATAAGGTTAAAATAG
GCTATCCATTAATTCTTGAA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MDFIKSLVENKLLLIGNFVLTSGKISPYYLDLRRLSNYYEIFSDTVNKAIDKIKNIDFDMIIGIATGGIPFASFISCRMG
KPLGYIRMEKKGYGTDKILEADVSGRKILLVDDVATTGGSLSKAVEIINSEGGRVVASLVIVDREEGAEKKLGEYGVKLI
SVYKIREILEYLLNSSLISDNDKNNIKEYLVKNIE

Sequences:

>Translated_195_residues
MDFIKSLVENKLLLIGNFVLTSGKISPYYLDLRRLSNYYEIFSDTVNKAIDKIKNIDFDMIIGIATGGIPFASFISCRMG
KPLGYIRMEKKGYGTDKILEADVSGRKILLVDDVATTGGSLSKAVEIINSEGGRVVASLVIVDREEGAEKKLGEYGVKLI
SVYKIREILEYLLNSSLISDNDKNNIKEYLVKNIE
>Mature_195_residues
MDFIKSLVENKLLLIGNFVLTSGKISPYYLDLRRLSNYYEIFSDTVNKAIDKIKNIDFDMIIGIATGGIPFASFISCRMG
KPLGYIRMEKKGYGTDKILEADVSGRKILLVDDVATTGGSLSKAVEIINSEGGRVVASLVIVDREEGAEKKLGEYGVKLI
SVYKIREILEYLLNSSLISDNDKNNIKEYLVKNIE

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=182, Percent_Identity=31.3186813186813, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1790073, Length=203, Percent_Identity=26.1083743842365, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508631, Length=194, Percent_Identity=29.8969072164948, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17554964, Length=196, Percent_Identity=31.1224489795918, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323530, Length=212, Percent_Identity=30.188679245283, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323927, Length=210, Percent_Identity=27.1428571428571, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17933654, Length=188, Percent_Identity=28.1914893617021, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_SULTO (Q970X1)

Other databases:

- EMBL:   AB104901
- EMBL:   BA000023
- RefSeq:   NP_377443.1
- ProteinModelPortal:   Q970X1
- SMR:   Q970X1
- GeneID:   1459516
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1481
- NMPDR:   fig|273063.1.peg.1611
- HOGENOM:   HBG404341
- OMA:   LSYPRIR
- ProtClustDB:   PRK00455
- BioCyc:   STOK273063:ST1481-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 21715; Mature: 21715

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 87-87 BINDING 91-91 BINDING 116-116 BINDING 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFIKSLVENKLLLIGNFVLTSGKISPYYLDLRRLSNYYEIFSDTVNKAIDKIKNIDFDM
CHHHHHHHCCCEEEEEHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHE
IIGIATGGIPFASFISCRMGKPLGYIRMEKKGYGTDKILEADVSGRKILLVDDVATTGGS
EEEEECCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCCEEEEEECCCCCCCH
LSKAVEIINSEGGRVVASLVIVDREEGAEKKLGEYGVKLISVYKIREILEYLLNSSLISD
HHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NDKNNIKEYLVKNIE
CCHHHHHHHHHHCCC
>Mature Secondary Structure
MDFIKSLVENKLLLIGNFVLTSGKISPYYLDLRRLSNYYEIFSDTVNKAIDKIKNIDFDM
CHHHHHHHCCCEEEEEHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHE
IIGIATGGIPFASFISCRMGKPLGYIRMEKKGYGTDKILEADVSGRKILLVDDVATTGGS
EEEEECCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCCEEEEEECCCCCCCH
LSKAVEIINSEGGRVVASLVIVDREEGAEKKLGEYGVKLISVYKIREILEYLLNSSLISD
HHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NDKNNIKEYLVKNIE
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479