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The map label for this gene is pdxT

Identifier: 15921733

GI number: 15921733

Start: 1447239

End: 1447841

Strand: Direct

Name: pdxT

Synonym: ST1442

Alternate gene names: 15921733

Gene position: 1447239-1447841 (Clockwise)

Preceding gene: 15921732

Following gene: 229310097

Centisome position: 53.71

GC content: 34.16

Gene sequence:

>603_bases
ATGAAAATTGGAATTGTTGCATATCAAGGTAGCTTTGAAGAACATGCGTTACAGACTAAAAGAGCTTTGGACAATTTGAA
AATTCAAGGAGATATAGTTGCTGTGAAAAAACCTAATGATTTGAAAGATGTTGATGCTATAATAATACCTGGCGGAGAGA
GTACAACCATTGGCGTTGTTGCTCAAAAACTTGGTATTTTAGATGAATTAAAAGAGAAAATAAATTCTGGGATACCAACT
TTAGGTACTTGTGCTGGAGCAATAATTTTAGCAAAAGATGTTACAGACGCCAAAGTCGGTAAAAAATCTCAGCCGTTAAT
TGGTTCAATGGATATTTCTGTGATTAGAAACTATTATGGTAGACAAAGAGAAAGTTTTGAAGCAACTGTTGATTTATCAG
AAATAGGGGGAGGAAAGACTAGAGTTGTGTTTATAAGAGCTCCTGCTATAGTCAAAACATGGGGAGATGCAAAGCCATTA
TCAAAACTTAATGATGTAATAATTATGGCTATGGAGAGAAATATGGTTGCTACAACATTTCATCCAGAGTTATCTTCAAC
TACTGTAATTCACGAGTTTCTCATTAAAATGGCAAAGAAATAG

Upstream 100 bases:

>100_bases
TAGTTCTTGAAGCTCAAAAGATGATAAGCGAGAGTAAAAGCATGATGGGAATTGATATTAAAGCATTGAAGCCAGAGGAA
TTACTTCAGGTGAGAGGTCA

Downstream 100 bases:

>100_bases
GTTTATTAGTTTTTAAATTAGATTAATTTTTAAGGGTTCAAACTATTGCCTCCGAAAGATCTTTTAATAGATAAGTCAGC
CTTAATACATGGCGTATCTA

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MKIGIVAYQGSFEEHALQTKRALDNLKIQGDIVAVKKPNDLKDVDAIIIPGGESTTIGVVAQKLGILDELKEKINSGIPT
LGTCAGAIILAKDVTDAKVGKKSQPLIGSMDISVIRNYYGRQRESFEATVDLSEIGGGKTRVVFIRAPAIVKTWGDAKPL
SKLNDVIIMAMERNMVATTFHPELSSTTVIHEFLIKMAKK

Sequences:

>Translated_200_residues
MKIGIVAYQGSFEEHALQTKRALDNLKIQGDIVAVKKPNDLKDVDAIIIPGGESTTIGVVAQKLGILDELKEKINSGIPT
LGTCAGAIILAKDVTDAKVGKKSQPLIGSMDISVIRNYYGRQRESFEATVDLSEIGGGKTRVVFIRAPAIVKTWGDAKPL
SKLNDVIIMAMERNMVATTFHPELSSTTVIHEFLIKMAKK
>Mature_200_residues
MKIGIVAYQGSFEEHALQTKRALDNLKIQGDIVAVKKPNDLKDVDAIIIPGGESTTIGVVAQKLGILDELKEKINSGIPT
LGTCAGAIILAKDVTDAKVGKKSQPLIGSMDISVIRNYYGRQRESFEATVDLSEIGGGKTRVVFIRAPAIVKTWGDAKPL
SKLNDVIIMAMERNMVATTFHPELSSTTVIHEFLIKMAKK

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=207, Percent_Identity=37.1980676328502, Blast_Score=117, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6323995, Length=212, Percent_Identity=31.6037735849057, Blast_Score=99, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321048, Length=213, Percent_Identity=31.924882629108, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_SULTO (Q971B2)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377402.1
- ProteinModelPortal:   Q971B2
- GeneID:   1459475
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1442
- NMPDR:   fig|273063.1.peg.1570
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13527
- BioCyc:   STOK273063:ST1442-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21660; Mature: 21660

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 84-84 ACT_SITE 181-181 ACT_SITE 183-183 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIVAYQGSFEEHALQTKRALDNLKIQGDIVAVKKPNDLKDVDAIIIPGGESTTIGVV
CEEEEEEECCCHHHHHHHHHHHHHCEEEECCEEEEECCCCCCCCCEEEECCCCCCHHHHH
AQKLGILDELKEKINSGIPTLGTCAGAIILAKDVTDAKVGKKSQPLIGSMDISVIRNYYG
HHHHCHHHHHHHHHHCCCCCHHHHHCEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHC
RQRESFEATVDLSEIGGGKTRVVFIRAPAIVKTWGDAKPLSKLNDVIIMAMERNMVATTF
CCCCCCEEEEEHHHCCCCCEEEEEEECCEEEECCCCCCCHHHHCCEEEEEECCCEEEEEE
HPELSSTTVIHEFLIKMAKK
CCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIGIVAYQGSFEEHALQTKRALDNLKIQGDIVAVKKPNDLKDVDAIIIPGGESTTIGVV
CEEEEEEECCCHHHHHHHHHHHHHCEEEECCEEEEECCCCCCCCCEEEECCCCCCHHHHH
AQKLGILDELKEKINSGIPTLGTCAGAIILAKDVTDAKVGKKSQPLIGSMDISVIRNYYG
HHHHCHHHHHHHHHHCCCCCHHHHHCEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHC
RQRESFEATVDLSEIGGGKTRVVFIRAPAIVKTWGDAKPLSKLNDVIIMAMERNMVATTF
CCCCCCEEEEEHHHCCCCCEEEEEEECCEEEECCCCCCCHHHHCCEEEEEECCCEEEEEE
HPELSSTTVIHEFLIKMAKK
CCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479