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The map label for this gene is gltX

Identifier: 15921712

GI number: 15921712

Start: 1426222

End: 1427922

Strand: Direct

Name: gltX

Synonym: ST1423

Alternate gene names: 15921712

Gene position: 1426222-1427922 (Clockwise)

Preceding gene: 15921711

Following gene: 15921713

Centisome position: 52.93

GC content: 34.1

Gene sequence:

>1701_bases
ATGGAAGAAATTGAGGAATTAATATATAAATACGCGCTACAAAATGCTTATAAACACGGTGGTAAAGCGCAAGATAAAGC
TGTTGTAAGTAAAATTTTTGTTGAGCGTCCTGATTTAAGATCTAGAGCTAAGGAAATATCTGAGATAGCAAAGAAAATAG
TAGAAAAAGTAAATTCAATGAGTATTCAAGACCAAGAGAGAGAACTTAAAGAAAAATATCCAGATTTACTCGAAGAGAAA
AAAAAGGAAGAACCAGAAAAGAAGACTCTTCCTCCAATTAAAGTTGAAGGGAAATTTGTTACAAGATTTGCGCCAAACCC
AGATGGTCCAATTCACTTAGGCAATGCTAGAGCGGCTATTATATCTTATAAATATGCTGAAATGTATAAAGGTGAGTTTA
TCCTAAGGTTTGACGATACTGATCCAAAGGTTAAAAAGCCAATAAAAGAGGCGTACGATTGGATTAGGAAAGATCTTAAA
TGGTTAGGTATAAAGTGGGATAAGGAAGTTAAAGCGTCAGAAAGACTAGAATTCTATTACAGTATGGCTAAAGAGCTTAT
AAGTAAAGGTTTTGCATATGTTGATACTTGCTCAGAGGAAGAGTTCAAAAAAATGAGAGATGCTAGTAAACCTTGTCCAA
ATAGGTTAAAATCAGCGGAGGATAATCTTTACTTATTTGAGAAGATGCTTAATGGAGAGTTTAAAGAAGGAGAAGCTGTA
GTAAGGATAAAAACTGATCTCTCTTTACCTGATCCATCGCAAAGAGATTGGGTTTTACTTCGAATAATAGATGTTAAGAA
AAACCCTCATCCTATCACTGGAGATAAATATTGGATTTGGCCTACTTATAATTTCGCAAGTGCCTTAGACGATCATGACT
TAGGTATAACTCACATTTTCAGAGGTAAAGAACATGAAGTAAATGCAGAAAAACAGAAATGGATATACAACTACATGGGC
TGGAAATATCCTTATGTTGAAGAATTTGGTAGGCTTAGATTAGAAGGATTTATGATGAGTAAATCAAAGATTAGAACAGT
ATTAGAGAAAGGCGTAAGCATTGATGATCCCAGATTACCTACGTTGGCTGGATTGAGGAGAAGAGGAATTCTACCAGAAA
CAATAATAGAAACTATAATTACAGTAGGTTTAAAGGTTAGTGATGCAACTATAAGTTTTGACAATTTAGCTGCACTTAAT
AGAAAGAAGTTGGATCCAATAGCTAAGAGACTTATGTTCGTTCAACAGCCTAAGGAGTTTATTATTGAGTTATCAGAACC
AATTAGGGCAAAAATACCATATAACCCTAGTAAACCTTCAGAATATAGAGAAATTCTAGTAAATCCTGGAGATAAGATTC
TTCTTGACGCGAAGGATGCAGAAGAAGGTGCGGTAGTAAGGTTAATGGAACTTTGCAATGTCACAATTTCTGGAAATAAA
TTAATATTTCATAGCAAAACTTTGGAAGACGCGAAGAAATTAAATGCAAAAATCATACAATGGGTAAAAAAAGATGAAGC
TTCTAACGTTGAGGTAATTATACCAGATCCGAATGAAGAGAAGCCACCTATATCCGGTTATGGAGAAAAAGAAATTGGGA
ATCTAAAAATCAATGAAATAGTACAGTTCATAAGATTTGGGTTCGTTAGGGTCGATAATAAGATCGATAATAAGGTTACC
GTAATATTCTCACATGAGTAA

Upstream 100 bases:

>100_bases
AGTGATGAAGAAGTTAAAAAGGTAGCTAACTATGTTACTTTAAATAATAGTGGAAAAGGAGTAGTTGAAATCGTAGATAA
GATATTAAAAGGTGAGTTAG

Downstream 100 bases:

>100_bases
CTTTATTAACCTAATAAGTTAATTCTTTTTTGCGTGACGAAATAAGAGGAGGATGAACGCATGTCTAAACCCTCATATGT
TAAATTCGATGTACCAGAGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MEEIEELIYKYALQNAYKHGGKAQDKAVVSKIFVERPDLRSRAKEISEIAKKIVEKVNSMSIQDQERELKEKYPDLLEEK
KKEEPEKKTLPPIKVEGKFVTRFAPNPDGPIHLGNARAAIISYKYAEMYKGEFILRFDDTDPKVKKPIKEAYDWIRKDLK
WLGIKWDKEVKASERLEFYYSMAKELISKGFAYVDTCSEEEFKKMRDASKPCPNRLKSAEDNLYLFEKMLNGEFKEGEAV
VRIKTDLSLPDPSQRDWVLLRIIDVKKNPHPITGDKYWIWPTYNFASALDDHDLGITHIFRGKEHEVNAEKQKWIYNYMG
WKYPYVEEFGRLRLEGFMMSKSKIRTVLEKGVSIDDPRLPTLAGLRRRGILPETIIETIITVGLKVSDATISFDNLAALN
RKKLDPIAKRLMFVQQPKEFIIELSEPIRAKIPYNPSKPSEYREILVNPGDKILLDAKDAEEGAVVRLMELCNVTISGNK
LIFHSKTLEDAKKLNAKIIQWVKKDEASNVEVIIPDPNEEKPPISGYGEKEIGNLKINEIVQFIRFGFVRVDNKIDNKVT
VIFSHE

Sequences:

>Translated_566_residues
MEEIEELIYKYALQNAYKHGGKAQDKAVVSKIFVERPDLRSRAKEISEIAKKIVEKVNSMSIQDQERELKEKYPDLLEEK
KKEEPEKKTLPPIKVEGKFVTRFAPNPDGPIHLGNARAAIISYKYAEMYKGEFILRFDDTDPKVKKPIKEAYDWIRKDLK
WLGIKWDKEVKASERLEFYYSMAKELISKGFAYVDTCSEEEFKKMRDASKPCPNRLKSAEDNLYLFEKMLNGEFKEGEAV
VRIKTDLSLPDPSQRDWVLLRIIDVKKNPHPITGDKYWIWPTYNFASALDDHDLGITHIFRGKEHEVNAEKQKWIYNYMG
WKYPYVEEFGRLRLEGFMMSKSKIRTVLEKGVSIDDPRLPTLAGLRRRGILPETIIETIITVGLKVSDATISFDNLAALN
RKKLDPIAKRLMFVQQPKEFIIELSEPIRAKIPYNPSKPSEYREILVNPGDKILLDAKDAEEGAVVRLMELCNVTISGNK
LIFHSKTLEDAKKLNAKIIQWVKKDEASNVEVIIPDPNEEKPPISGYGEKEIGNLKINEIVQFIRFGFVRVDNKIDNKVT
VIFSHE
>Mature_566_residues
MEEIEELIYKYALQNAYKHGGKAQDKAVVSKIFVERPDLRSRAKEISEIAKKIVEKVNSMSIQDQERELKEKYPDLLEEK
KKEEPEKKTLPPIKVEGKFVTRFAPNPDGPIHLGNARAAIISYKYAEMYKGEFILRFDDTDPKVKKPIKEAYDWIRKDLK
WLGIKWDKEVKASERLEFYYSMAKELISKGFAYVDTCSEEEFKKMRDASKPCPNRLKSAEDNLYLFEKMLNGEFKEGEAV
VRIKTDLSLPDPSQRDWVLLRIIDVKKNPHPITGDKYWIWPTYNFASALDDHDLGITHIFRGKEHEVNAEKQKWIYNYMG
WKYPYVEEFGRLRLEGFMMSKSKIRTVLEKGVSIDDPRLPTLAGLRRRGILPETIIETIITVGLKVSDATISFDNLAALN
RKKLDPIAKRLMFVQQPKEFIIELSEPIRAKIPYNPSKPSEYREILVNPGDKILLDAKDAEEGAVVRLMELCNVTISGNK
LIFHSKTLEDAKKLNAKIIQWVKKDEASNVEVIIPDPNEEKPPISGYGEKEIGNLKINEIVQFIRFGFVRVDNKIDNKVT
VIFSHE

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=425, Percent_Identity=33.1764705882353, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI4826960, Length=328, Percent_Identity=37.8048780487805, Blast_Score=179, Evalue=7e-45,
Organism=Homo sapiens, GI134288884, Length=304, Percent_Identity=27.6315789473684, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786895, Length=508, Percent_Identity=33.4645669291339, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1788743, Length=308, Percent_Identity=26.6233766233766, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17511007, Length=502, Percent_Identity=30.0796812749004, Blast_Score=199, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17543332, Length=371, Percent_Identity=35.3099730458221, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71993394, Length=312, Percent_Identity=28.525641025641, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324742, Length=382, Percent_Identity=36.3874345549738, Blast_Score=223, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6321192, Length=512, Percent_Identity=29.1015625, Blast_Score=187, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6324540, Length=297, Percent_Identity=31.3131313131313, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24649466, Length=415, Percent_Identity=33.0120481927711, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI17864488, Length=505, Percent_Identity=29.9009900990099, Blast_Score=175, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24665321, Length=299, Percent_Identity=27.7591973244147, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_SULTO (Q971D0)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377381.1
- ProteinModelPortal:   Q971D0
- SMR:   Q971D0
- GeneID:   1459453
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1423
- NMPDR:   fig|273063.1.peg.1549
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- ProtClustDB:   PRK04156
- BioCyc:   STOK273063:ST1423-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 65517; Mature: 65517

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIEELIYKYALQNAYKHGGKAQDKAVVSKIFVERPDLRSRAKEISEIAKKIVEKVNSM
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
SIQDQERELKEKYPDLLEEKKKEEPEKKTLPPIKVEGKFVTRFAPNPDGPIHLGNARAAI
CCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEECCEEEEEECCCCCCCEEECCCCEEE
ISYKYAEMYKGEFILRFDDTDPKVKKPIKEAYDWIRKDLKWLGIKWDKEVKASERLEFYY
EEEHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SMAKELISKGFAYVDTCSEEEFKKMRDASKPCPNRLKSAEDNLYLFEKMLNGEFKEGEAV
HHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCEE
VRIKTDLSLPDPSQRDWVLLRIIDVKKNPHPITGDKYWIWPTYNFASALDDHDLGITHIF
EEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCHHHHCCCCCCCEEEEE
RGKEHEVNAEKQKWIYNYMGWKYPYVEEFGRLRLEGFMMSKSKIRTVLEKGVSIDDPRLP
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TLAGLRRRGILPETIIETIITVGLKVSDATISFDNLAALNRKKLDPIAKRLMFVQQPKEF
HHHHHHHCCCCHHHHHHHHHHHCCEECCCEECHHHHHHHHHHHCCHHHHHHHHHHCCHHH
IIELSEPIRAKIPYNPSKPSEYREILVNPGDKILLDAKDAEEGAVVRLMELCNVTISGNK
HHCCCCCCEEECCCCCCCCHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHCEEECCCE
LIFHSKTLEDAKKLNAKIIQWVKKDEASNVEVIIPDPNEEKPPISGYGEKEIGNLKINEI
EEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
VQFIRFGFVRVDNKIDNKVTVIFSHE
HHHHHHHHEEECCCCCCEEEEEEECC
>Mature Secondary Structure
MEEIEELIYKYALQNAYKHGGKAQDKAVVSKIFVERPDLRSRAKEISEIAKKIVEKVNSM
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
SIQDQERELKEKYPDLLEEKKKEEPEKKTLPPIKVEGKFVTRFAPNPDGPIHLGNARAAI
CCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEECCEEEEEECCCCCCCEEECCCCEEE
ISYKYAEMYKGEFILRFDDTDPKVKKPIKEAYDWIRKDLKWLGIKWDKEVKASERLEFYY
EEEHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SMAKELISKGFAYVDTCSEEEFKKMRDASKPCPNRLKSAEDNLYLFEKMLNGEFKEGEAV
HHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCEE
VRIKTDLSLPDPSQRDWVLLRIIDVKKNPHPITGDKYWIWPTYNFASALDDHDLGITHIF
EEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCHHHHCCCCCCCEEEEE
RGKEHEVNAEKQKWIYNYMGWKYPYVEEFGRLRLEGFMMSKSKIRTVLEKGVSIDDPRLP
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TLAGLRRRGILPETIIETIITVGLKVSDATISFDNLAALNRKKLDPIAKRLMFVQQPKEF
HHHHHHHCCCCHHHHHHHHHHHCCEECCCEECHHHHHHHHHHHCCHHHHHHHHHHCCHHH
IIELSEPIRAKIPYNPSKPSEYREILVNPGDKILLDAKDAEEGAVVRLMELCNVTISGNK
HHCCCCCCEEECCCCCCCCHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHCEEECCCE
LIFHSKTLEDAKKLNAKIIQWVKKDEASNVEVIIPDPNEEKPPISGYGEKEIGNLKINEI
EEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
VQFIRFGFVRVDNKIDNKVTVIFSHE
HHHHHHHHEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479