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The map label for this gene is pckG

Identifier: 15921309

GI number: 15921309

Start: 1043999

End: 1045807

Strand: Direct

Name: pckG

Synonym: ST1058

Alternate gene names: 15921309

Gene position: 1043999-1045807 (Clockwise)

Preceding gene: 15921307

Following gene: 15921313

Centisome position: 38.74

GC content: 32.84

Gene sequence:

>1809_bases
ATGTATATTATGAGATCAAGTTTTAATTTTTTAAACTTGTTAGTTTCAGATAAAATGATTAAAAAAATAGAAATATTAAA
TAATTCGTATGTAGAGGAATTTATTGAAAAAACTGCTGAATTATGCAATCCCTCATCAATATATCTAGTTACCTCAGAAG
AAGACAAAGAGTATATACGAAGAAAAGCAATAGAAACAAAAGAAGAATTACCGTTAAAAACTTTAGGGCATACAATACAT
TTTGATCACCCCTTAGACCAGGCTAGAGCTAGAGATGATACATTTATACTTACTGATGATAAAATTCCGTTTGTAAATAC
TAAAAAGAGGGAAGATGGAATAAGAGAGGTTCTCTCTCTGTTAAAGGACTCAATGAAAGGAAGGGAGATGTATGTGGGAT
TTTACTCATTAGGTCCTAAAAATTCAATTTTTCAGCATTTAGCTATTCAAATATCAGATTCGCCCTATGTAATTCATAGT
GAGAACATTCTTTATAGACTCGATTTTGATGATTTTAAAGGAAATAAGCAATTTTTGAAATTCGTTCATTCAAAAGGGGA
GTTGGATATAAAAAAGAGGAGAATTATGATAGATCTTAAGGAAGATACTGTTTATAGCGTAAACACAACTTATGCTGGCA
ATAGCGTTGGGTTGAAGAAATTAGCGTTAAGATTAACTATTGCTAAGGCTGTAAAAGAGGGTTGGTTATCAGAACATATG
GCGATTATAGGCTTTGAAGGTGATAAAGGAACTCATTATTTTACGGCTTCTTTTCCATCTGGTAGCGGAAAGACATCAAC
GTCTATGATGGGGAAACTAATAAGTGATGACCTTGCCTTTATAAGGGAGATTAATGGAGTAGCTAGAGCTGTTAATCCGG
AGATAGGCGTATTTGGGATAATTCAAGGGATTAACTCTAAGGACGATCCTATAATATGGGAAGTTTTGCATAAGCCTAAT
GAGGTGATATTTTCAAATGTATTAATGACAGAGGACGGTTATGTATATTGGGAAGGTAGTGATGATAAGAGACCGGAAAG
GGGGATAAATTATGAAGGTTTTTGGAGTAATGACATGAATAAACCTGCATCTCATCCGAATGCAAGGTTTACAGTACCTT
TAACAGCTTTTAAGAACCTTGATGAAAATTACGATAATCCAGATGGGGTCGAAATCGAGGGAATAATATTTGGTGTAAGA
GATTATGATACGCTAATTCCGGTTGTTGAAGCTTTTTCTTGTTCTCATGGAATTGTAACTATTGGTGCATCAATGGAATC
AGCAAGGACTAGTGCTGTGATAGGAAAAGGAGACGAATATGAGTTTAATCCTATGGCTATACTAGATTTTATGCCAATTC
ATTTAGGAGAATATTTAGGAAATTATCTAGAATTTTGTAAAAAACTTAAAAAAGTTCCTAGAATTTTCGGGTTTAATTAT
TTTCTTAAAGAGGGGAATAGGTTTCTTAATTCAAAAGAAGACAAAAGGGTTTGGATAAAATGGGCTGTAAAAAGGGTTGA
GGGAAGTGTAGATGTAATTTATACACCTATTGGGCTAGTTCCCTATTATGAAGATTTAAAGACATTATTTAGGGAGATGT
TAAATAAAGAATATACGATAGAAGATTATGAGAAGCAGTTTACTCTTAAACTTGACAAGTATTTAGAGAAAAATGAAAGG
ATTCTCAAATTATATAAGGAAATCCTGGCTCCAAAAGAAGTTATAGCAGAATTAGAACAACAGAAGGAAAGGATTCTCAA
ATATATAGATAAATACGGTAAAAGAATATCTCCATTGGACCTAAGATGA

Upstream 100 bases:

>100_bases
CAAACAAGGTATATCAGTATTGGGCGCTACTTCTTATAACATTGTACTTTAAGCCTAATTAAACGTGTTAGTTAGAAAGA
TTTATATCATTTAATACAAT

Downstream 100 bases:

>100_bases
GAAAAAAGTAAAAAATAAAATTTTACCTTTTCATTTGCATTCAGGAGTTCCCTTCACTGTTCTCTTCTTCATATTTTAGC
ACTAGAAGAGGTGCATTACT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MYIMRSSFNFLNLLVSDKMIKKIEILNNSYVEEFIEKTAELCNPSSIYLVTSEEDKEYIRRKAIETKEELPLKTLGHTIH
FDHPLDQARARDDTFILTDDKIPFVNTKKREDGIREVLSLLKDSMKGREMYVGFYSLGPKNSIFQHLAIQISDSPYVIHS
ENILYRLDFDDFKGNKQFLKFVHSKGELDIKKRRIMIDLKEDTVYSVNTTYAGNSVGLKKLALRLTIAKAVKEGWLSEHM
AIIGFEGDKGTHYFTASFPSGSGKTSTSMMGKLISDDLAFIREINGVARAVNPEIGVFGIIQGINSKDDPIIWEVLHKPN
EVIFSNVLMTEDGYVYWEGSDDKRPERGINYEGFWSNDMNKPASHPNARFTVPLTAFKNLDENYDNPDGVEIEGIIFGVR
DYDTLIPVVEAFSCSHGIVTIGASMESARTSAVIGKGDEYEFNPMAILDFMPIHLGEYLGNYLEFCKKLKKVPRIFGFNY
FLKEGNRFLNSKEDKRVWIKWAVKRVEGSVDVIYTPIGLVPYYEDLKTLFREMLNKEYTIEDYEKQFTLKLDKYLEKNER
ILKLYKEILAPKEVIAELEQQKERILKYIDKYGKRISPLDLR

Sequences:

>Translated_602_residues
MYIMRSSFNFLNLLVSDKMIKKIEILNNSYVEEFIEKTAELCNPSSIYLVTSEEDKEYIRRKAIETKEELPLKTLGHTIH
FDHPLDQARARDDTFILTDDKIPFVNTKKREDGIREVLSLLKDSMKGREMYVGFYSLGPKNSIFQHLAIQISDSPYVIHS
ENILYRLDFDDFKGNKQFLKFVHSKGELDIKKRRIMIDLKEDTVYSVNTTYAGNSVGLKKLALRLTIAKAVKEGWLSEHM
AIIGFEGDKGTHYFTASFPSGSGKTSTSMMGKLISDDLAFIREINGVARAVNPEIGVFGIIQGINSKDDPIIWEVLHKPN
EVIFSNVLMTEDGYVYWEGSDDKRPERGINYEGFWSNDMNKPASHPNARFTVPLTAFKNLDENYDNPDGVEIEGIIFGVR
DYDTLIPVVEAFSCSHGIVTIGASMESARTSAVIGKGDEYEFNPMAILDFMPIHLGEYLGNYLEFCKKLKKVPRIFGFNY
FLKEGNRFLNSKEDKRVWIKWAVKRVEGSVDVIYTPIGLVPYYEDLKTLFREMLNKEYTIEDYEKQFTLKLDKYLEKNER
ILKLYKEILAPKEVIAELEQQKERILKYIDKYGKRISPLDLR
>Mature_602_residues
MYIMRSSFNFLNLLVSDKMIKKIEILNNSYVEEFIEKTAELCNPSSIYLVTSEEDKEYIRRKAIETKEELPLKTLGHTIH
FDHPLDQARARDDTFILTDDKIPFVNTKKREDGIREVLSLLKDSMKGREMYVGFYSLGPKNSIFQHLAIQISDSPYVIHS
ENILYRLDFDDFKGNKQFLKFVHSKGELDIKKRRIMIDLKEDTVYSVNTTYAGNSVGLKKLALRLTIAKAVKEGWLSEHM
AIIGFEGDKGTHYFTASFPSGSGKTSTSMMGKLISDDLAFIREINGVARAVNPEIGVFGIIQGINSKDDPIIWEVLHKPN
EVIFSNVLMTEDGYVYWEGSDDKRPERGINYEGFWSNDMNKPASHPNARFTVPLTAFKNLDENYDNPDGVEIEGIIFGVR
DYDTLIPVVEAFSCSHGIVTIGASMESARTSAVIGKGDEYEFNPMAILDFMPIHLGEYLGNYLEFCKKLKKVPRIFGFNY
FLKEGNRFLNSKEDKRVWIKWAVKRVEGSVDVIYTPIGLVPYYEDLKTLFREMLNKEYTIEDYEKQFTLKLDKYLEKNER
ILKLYKEILAPKEVIAELEQQKERILKYIDKYGKRISPLDLR

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI187281517, Length=606, Percent_Identity=32.6732673267327, Blast_Score=266, Evalue=5e-71,
Organism=Homo sapiens, GI66346721, Length=630, Percent_Identity=31.1111111111111, Blast_Score=253, Evalue=5e-67,
Organism=Homo sapiens, GI66346723, Length=377, Percent_Identity=30.2387267904509, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71989645, Length=641, Percent_Identity=29.6411856474259, Blast_Score=241, Evalue=9e-64,
Organism=Caenorhabditis elegans, GI71989658, Length=607, Percent_Identity=30.1482701812191, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI71989653, Length=552, Percent_Identity=30.6159420289855, Blast_Score=233, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17555492, Length=558, Percent_Identity=30.6451612903226, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI32564411, Length=565, Percent_Identity=30.2654867256637, Blast_Score=229, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI71989649, Length=520, Percent_Identity=30.1923076923077, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI32564407, Length=456, Percent_Identity=31.359649122807, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI32564409, Length=309, Percent_Identity=31.7152103559871, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24655082, Length=632, Percent_Identity=31.3291139240506, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI161077226, Length=552, Percent_Identity=33.1521739130435, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24655085, Length=542, Percent_Identity=31.7343173431734, Blast_Score=248, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24655088, Length=499, Percent_Identity=31.6633266533066, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_SULTO (Q972S7)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376978.1
- ProteinModelPortal:   Q972S7
- GeneID:   1459041
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1058
- NMPDR:   fig|273063.1.peg.1146
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- BioCyc:   STOK273063:ST1058-MONOMER
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 69439; Mature: 69439

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 262-262 BINDING 89-89 BINDING 213-213 BINDING 220-220 BINDING 260-260 BINDING 369-369 BINDING 400-400

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIMRSSFNFLNLLVSDKMIKKIEILNNSYVEEFIEKTAELCNPSSIYLVTSEEDKEYIR
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
RKAIETKEELPLKTLGHTIHFDHPLDQARARDDTFILTDDKIPFVNTKKREDGIREVLSL
HHHHCCHHHCCHHHHCCEEECCCCHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
LKDSMKGREMYVGFYSLGPKNSIFQHLAIQISDSPYVIHSENILYRLDFDDFKGNKQFLK
HHHCCCCCEEEEEEEECCCCHHHHHEEEEEECCCCEEEECCCEEEEECCHHCCCCHHHHH
FVHSKGELDIKKRRIMIDLKEDTVYSVNTTYAGNSVGLKKLALRLTIAKAVKEGWLSEHM
HHHCCCCCEEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCE
AIIGFEGDKGTHYFTASFPSGSGKTSTSMMGKLISDDLAFIREINGVARAVNPEIGVFGI
EEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
IQGINSKDDPIIWEVLHKPNEVIFSNVLMTEDGYVYWEGSDDKRPERGINYEGFWSNDMN
EECCCCCCCCEEHEECCCCCHHHHHHEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCC
KPASHPNARFTVPLTAFKNLDENYDNPDGVEIEGIIFGVRDYDTLIPVVEAFSCSHGIVT
CCCCCCCCEEEEEHHHHHCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCEEE
IGASMESARTSAVIGKGDEYEFNPMAILDFMPIHLGEYLGNYLEFCKKLKKVPRIFGFNY
EECCHHHCCCCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
FLKEGNRFLNSKEDKRVWIKWAVKRVEGSVDVIYTPIGLVPYYEDLKTLFREMLNKEYTI
HHHCCCHHCCCCCCCEEHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
EDYEKQFTLKLDKYLEKNERILKLYKEILAPKEVIAELEQQKERILKYIDKYGKRISPLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LR
CC
>Mature Secondary Structure
MYIMRSSFNFLNLLVSDKMIKKIEILNNSYVEEFIEKTAELCNPSSIYLVTSEEDKEYIR
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
RKAIETKEELPLKTLGHTIHFDHPLDQARARDDTFILTDDKIPFVNTKKREDGIREVLSL
HHHHCCHHHCCHHHHCCEEECCCCHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
LKDSMKGREMYVGFYSLGPKNSIFQHLAIQISDSPYVIHSENILYRLDFDDFKGNKQFLK
HHHCCCCCEEEEEEEECCCCHHHHHEEEEEECCCCEEEECCCEEEEECCHHCCCCHHHHH
FVHSKGELDIKKRRIMIDLKEDTVYSVNTTYAGNSVGLKKLALRLTIAKAVKEGWLSEHM
HHHCCCCCEEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCE
AIIGFEGDKGTHYFTASFPSGSGKTSTSMMGKLISDDLAFIREINGVARAVNPEIGVFGI
EEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
IQGINSKDDPIIWEVLHKPNEVIFSNVLMTEDGYVYWEGSDDKRPERGINYEGFWSNDMN
EECCCCCCCCEEHEECCCCCHHHHHHEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCC
KPASHPNARFTVPLTAFKNLDENYDNPDGVEIEGIIFGVRDYDTLIPVVEAFSCSHGIVT
CCCCCCCCEEEEEHHHHHCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCEEE
IGASMESARTSAVIGKGDEYEFNPMAILDFMPIHLGEYLGNYLEFCKKLKKVPRIFGFNY
EECCHHHCCCCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
FLKEGNRFLNSKEDKRVWIKWAVKRVEGSVDVIYTPIGLVPYYEDLKTLFREMLNKEYTI
HHHCCCHHCCCCCCCEEHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
EDYEKQFTLKLDKYLEKNERILKLYKEILAPKEVIAELEQQKERILKYIDKYGKRISPLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479