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The map label for this gene is truB

Identifier: 15920607

GI number: 15920607

Start: 414842

End: 415555

Strand: Direct

Name: truB

Synonym: ST0402

Alternate gene names: 15920607

Gene position: 414842-415555 (Clockwise)

Preceding gene: 15920606

Following gene: 15920608

Centisome position: 15.39

GC content: 31.51

Gene sequence:

>714_bases
ATGTCATATATTAGTTCTTCTGGGAAAGAATATGTATGCTTAATGCAAGTACATTGTGATTTTAATATAGATGAACTTAA
ACAAATAATTTCAAAATTTATAGGTATAATATATCAGAAACCACCCGTAAGATCCTCAGTGAAGAGAAGAACAAGAAAAA
AGAAAATTTATGATATAGAGATACTTGATACGGATAAGAGATTTATTTTACTTAGGATATCCTCTGATCCTGGCACATAT
ATGAGAAAATTATGTCATGATATTGGAGTAATATTAGGTTGCGGTGCACATATGAGAGAACTAAGGAGAATTAGATCTGG
GATATTTACAGAGAAAAACTTAGTTACATTACAAGAGATTTCAGAAGCTCTTTATATGTGGAAAAACTGTAAAGATGAGA
GTGATTTAAGAAAAATATTATTACCTATGGAATATGCTACTTGTGGTATGCCTAAAATATTAATTGATGATAATGCTGTT
GATGCCATATCTTATGGTGCAATGCTTACAGCACCTGGAATAGTAGCTTATCAAAGGTTTAGGGTAAAGGATACTGTAGC
GATATTAACACTAAAAGGAGAGCTAGTAGCTATAGGTGAAGCTGATGTAGATTCTCAAAAATTAGTAGATATGAAAAAAG
GAATAGTAGTTAAGCCTAAAAGAGTTCTTATGCCTCGTGATATATATCCTAGATCGTGGAAGAAACATGGATGA

Upstream 100 bases:

>100_bases
CATGGGGGGACCCTAGAGCCTTGAGGGTTAAACCCTCAGGCGGGGAGACCCTAAAGTAACTGGAGTATTACCAATTGGGT
TAGAAAATGCAACAAAACTA

Downstream 100 bases:

>100_bases
ATTTGTTGTTAATGATGTAGTAAATGGTATACCTTTATCATTGATAAGTAGTTATGGTGTTTTTTCTAAAAAGAAATTAG
ACCTTGGTACAAGAATGTTA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSYISSSGKEYVCLMQVHCDFNIDELKQIISKFIGIIYQKPPVRSSVKRRTRKKKIYDIEILDTDKRFILLRISSDPGTY
MRKLCHDIGVILGCGAHMRELRRIRSGIFTEKNLVTLQEISEALYMWKNCKDESDLRKILLPMEYATCGMPKILIDDNAV
DAISYGAMLTAPGIVAYQRFRVKDTVAILTLKGELVAIGEADVDSQKLVDMKKGIVVKPKRVLMPRDIYPRSWKKHG

Sequences:

>Translated_237_residues
MSYISSSGKEYVCLMQVHCDFNIDELKQIISKFIGIIYQKPPVRSSVKRRTRKKKIYDIEILDTDKRFILLRISSDPGTY
MRKLCHDIGVILGCGAHMRELRRIRSGIFTEKNLVTLQEISEALYMWKNCKDESDLRKILLPMEYATCGMPKILIDDNAV
DAISYGAMLTAPGIVAYQRFRVKDTVAILTLKGELVAIGEADVDSQKLVDMKKGIVVKPKRVLMPRDIYPRSWKKHG
>Mature_236_residues
SYISSSGKEYVCLMQVHCDFNIDELKQIISKFIGIIYQKPPVRSSVKRRTRKKKIYDIEILDTDKRFILLRISSDPGTYM
RKLCHDIGVILGCGAHMRELRRIRSGIFTEKNLVTLQEISEALYMWKNCKDESDLRKILLPMEYATCGMPKILIDDNAVD
AISYGAMLTAPGIVAYQRFRVKDTVAILTLKGELVAIGEADVDSQKLVDMKKGIVVKPKRVLMPRDIYPRSWKKHG

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI4503337, Length=233, Percent_Identity=36.0515021459227, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI215599015, Length=233, Percent_Identity=36.0515021459227, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17553978, Length=232, Percent_Identity=36.2068965517241, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6323204, Length=233, Percent_Identity=39.4849785407725, Blast_Score=179, Evalue=3e-46,
Organism=Drosophila melanogaster, GI281364189, Length=233, Percent_Identity=36.0515021459227, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI281364187, Length=233, Percent_Identity=36.0515021459227, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI281364185, Length=233, Percent_Identity=36.0515021459227, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI281364183, Length=233, Percent_Identity=36.0515021459227, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI62471759, Length=233, Percent_Identity=36.0515021459227, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI17975520, Length=233, Percent_Identity=36.0515021459227, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_SULTO (Q975L5)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376276.1
- ProteinModelPortal:   Q975L5
- SMR:   Q975L5
- GeneID:   1458329
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0402
- NMPDR:   fig|273063.1.peg.444
- HOGENOM:   HBG397258
- OMA:   IRTRRIH
- BioCyc:   STOK273063:ST0402-MONOMER
- HAMAP:   MF_01081
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947
- SMART:   SM00359
- TIGRFAMs:   TIGR00425

Pfam domain/function: PF01472 PUA; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 27147; Mature: 27015

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYISSSGKEYVCLMQVHCDFNIDELKQIISKFIGIIYQKPPVRSSVKRRTRKKKIYDIE
CCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEE
ILDTDKRFILLRISSDPGTYMRKLCHDIGVILGCGAHMRELRRIRSGIFTEKNLVTLQEI
EEECCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHH
SEALYMWKNCKDESDLRKILLPMEYATCGMPKILIDDNAVDAISYGAMLTAPGIVAYQRF
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCHHHCCCHHHHHHE
RVKDTVAILTLKGELVAIGEADVDSQKLVDMKKGIVVKPKRVLMPRDIYPRSWKKHG
EECCEEEEEEECCCEEEECCCCCCHHHHHHHHCCEEECCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SYISSSGKEYVCLMQVHCDFNIDELKQIISKFIGIIYQKPPVRSSVKRRTRKKKIYDIE
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEE
ILDTDKRFILLRISSDPGTYMRKLCHDIGVILGCGAHMRELRRIRSGIFTEKNLVTLQEI
EEECCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHH
SEALYMWKNCKDESDLRKILLPMEYATCGMPKILIDDNAVDAISYGAMLTAPGIVAYQRF
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCHHHCCCHHHHHHE
RVKDTVAILTLKGELVAIGEADVDSQKLVDMKKGIVVKPKRVLMPRDIYPRSWKKHG
EECCEEEEEEECCCEEEECCCCCCHHHHHHHHCCEEECCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479