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The map label for this gene is truB [H]

Identifier: 15920606

GI number: 15920606

Start: 414511

End: 414765

Strand: Direct

Name: truB [H]

Synonym: STS055

Alternate gene names: 15920606

Gene position: 414511-414765 (Clockwise)

Preceding gene: 15920605

Following gene: 15920607

Centisome position: 15.38

GC content: 29.8

Gene sequence:

>255_bases
ATGGAAATATATGATTTTATTTATAAAATAGATTCTTTTTGTAATTATAGTAATAACTGGAATATTATACAAGATTCCTA
TACTAATGAAAAATATGGTTACTTTGCTGATAAAAGACCCATAGAAGTTTTAATAAAAAACTCAATTATTAACGCTGATA
AACCACCTGGACCTACAAGTCATGAAGTAGCTTATTGGATAAAACAAATGTTTAAGGTTTCAAAGGCTGGTCATGGGGGG
ACCCTAGAGCCTTGA

Upstream 100 bases:

>100_bases
AAGGGAGCAAGTGATGATGAAGTTAAGAAAAAATTAGAAGAAGCGGGTCTTATAGAATTTATGAAACAAGATGTTAAGCC
AAAAATTCCTGTGATTTAAT

Downstream 100 bases:

>100_bases
GGGTTAAACCCTCAGGCGGGGAGACCCTAAAGTAACTGGAGTATTACCAATTGGGTTAGAAAATGCAACAAAACTAATGT
CATATATTAGTTCTTCTGGG

Product: centromere/microtubule-binding protein

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 84; Mature: 84

Protein sequence:

>84_residues
MEIYDFIYKIDSFCNYSNNWNIIQDSYTNEKYGYFADKRPIEVLIKNSIINADKPPGPTSHEVAYWIKQMFKVSKAGHGG
TLEP

Sequences:

>Translated_84_residues
MEIYDFIYKIDSFCNYSNNWNIIQDSYTNEKYGYFADKRPIEVLIKNSIINADKPPGPTSHEVAYWIKQMFKVSKAGHGG
TLEP
>Mature_84_residues
MEIYDFIYKIDSFCNYSNNWNIIQDSYTNEKYGYFADKRPIEVLIKNSIINADKPPGPTSHEVAYWIKQMFKVSKAGHGG
TLEP

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Drosophila melanogaster, GI281364189, Length=64, Percent_Identity=46.875, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364187, Length=64, Percent_Identity=46.875, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364185, Length=64, Percent_Identity=46.875, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364183, Length=64, Percent_Identity=46.875, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI62471759, Length=64, Percent_Identity=46.875, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17975520, Length=64, Percent_Identity=46.875, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 9755; Mature: 9755

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIYDFIYKIDSFCNYSNNWNIIQDSYTNEKYGYFADKRPIEVLIKNSIINADKPPGPTS
CCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHHEECCCCCCCCCCCCCC
HEVAYWIKQMFKVSKAGHGGTLEP
HHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEIYDFIYKIDSFCNYSNNWNIIQDSYTNEKYGYFADKRPIEVLIKNSIINADKPPGPTS
CCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHHEECCCCCCCCCCCCCC
HEVAYWIKQMFKVSKAGHGGTLEP
HHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]