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The map label for this gene is shiA [H]

Identifier: 15920538

GI number: 15920538

Start: 357765

End: 358862

Strand: Reverse

Name: shiA [H]

Synonym: ST0339

Alternate gene names: 15920538

Gene position: 358862-357765 (Counterclockwise)

Preceding gene: 15920543

Following gene: 15920537

Centisome position: 13.32

GC content: 28.42

Gene sequence:

>1098_bases
ATGAAAAGAGTAATCATAGGTAGTTTATTGTTAACAATATCACAATGGTATGCATTTTTCTTATTTTCTCAGTTTTCTTT
TATAGTATTTAGTGAATTTATAGGATTGTCTATATTTATAATGGGTTTTATAAGTAGGGCCTTAGGCAGTATTTTATTTG
GATACATAGGTGATAAAATTAGTAGAAGAACAGCATTATTGCTAACTGGAATTACGTTAATCATATCTTCATTAATTGTT
TTAATACCGAGTATATTTATTGTTTTTATTTCTAGGATTTTACAGGGTTTAAGTCTAGGAGGTGAATGGGGAGGTGCTAG
CACTGTAATAATAGAAACCTATTCAAGCCATAGACTTAGAGGAACTATTGCGAGTATAATTCAATTATCTGTACCTATAG
CTATAATACTTTCCTCTTCTACGCTTCTTCTAATTTTCTTATATTCAATTCCTTGGAGAGAATCTTTTCTAATTATAACT
GTTTTAACTCTAATAGCATTACCGTTAATTAGAGATGTAAACAAAGAAAAAATGTTCAGTAGTAAAATTCCATTATTTGA
GGCAATATTGAATGATTGGAAAAATATACTAAAATCTATTAGTATAAAAGTAAGTGAAAGTGCAGCTTTTTACGTTTTTT
CAGCTTATATTTTTTCTCTATATTCTAATTTGAAAGAGCTTCCTATAATAGTTTTAGAAGCAATTACATTTCAACTATTC
ACAATACCGCTTTTTGGTTTTTTATCAGATATTATTGGAAGAAGATTAGTAGTTATTATAGGTCTCATTATAATGGGAGT
TGGAAGTTATCTTTTATCATTGAAGATCATTTATTATGGAGAAATAGTTATATCTATCTCTGATTCTGCATTATATGCTC
CTCAATCCTCAATTTTTACTGAAATATTTAATAAAAAATATAGGTTTACTGCAGCTAATTTCTCATATCAACTAGCAAGT
ATACTAGGAGGTGCCTTAGCCCCTCTCTTAATTTCAGACTATTCTAACCTCTTTATATTCATAATGTTAAGCTACATAAC
CATTACATGTATAGGTGTATATTTCATAGAAGAAACGAAAGGGAAAGGAATAGCATAG

Upstream 100 bases:

>100_bases
TATTACCTTATAATATATTTAGATCTATGTTGCTAAGATTGATAATTTTTATAGATTGCAAATAAATATTAGTGAGAGTT
ACGCATTAAGATTTAGTTAT

Downstream 100 bases:

>100_bases
TTATATTATCGTCATTATGGTATTTATATTTCTATATTCACATTAGGATGTATAAAACCTTTTCTTAGCATCTTCTCCTA
TAGAGATTCTCATTAATTAA

Product: transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKRVIIGSLLLTISQWYAFFLFSQFSFIVFSEFIGLSIFIMGFISRALGSILFGYIGDKISRRTALLLTGITLIISSLIV
LIPSIFIVFISRILQGLSLGGEWGGASTVIIETYSSHRLRGTIASIIQLSVPIAIILSSSTLLLIFLYSIPWRESFLIIT
VLTLIALPLIRDVNKEKMFSSKIPLFEAILNDWKNILKSISIKVSESAAFYVFSAYIFSLYSNLKELPIIVLEAITFQLF
TIPLFGFLSDIIGRRLVVIIGLIIMGVGSYLLSLKIIYYGEIVISISDSALYAPQSSIFTEIFNKKYRFTAANFSYQLAS
ILGGALAPLLISDYSNLFIFIMLSYITITCIGVYFIEETKGKGIA

Sequences:

>Translated_365_residues
MKRVIIGSLLLTISQWYAFFLFSQFSFIVFSEFIGLSIFIMGFISRALGSILFGYIGDKISRRTALLLTGITLIISSLIV
LIPSIFIVFISRILQGLSLGGEWGGASTVIIETYSSHRLRGTIASIIQLSVPIAIILSSSTLLLIFLYSIPWRESFLIIT
VLTLIALPLIRDVNKEKMFSSKIPLFEAILNDWKNILKSISIKVSESAAFYVFSAYIFSLYSNLKELPIIVLEAITFQLF
TIPLFGFLSDIIGRRLVVIIGLIIMGVGSYLLSLKIIYYGEIVISISDSALYAPQSSIFTEIFNKKYRFTAANFSYQLAS
ILGGALAPLLISDYSNLFIFIMLSYITITCIGVYFIEETKGKGIA
>Mature_365_residues
MKRVIIGSLLLTISQWYAFFLFSQFSFIVFSEFIGLSIFIMGFISRALGSILFGYIGDKISRRTALLLTGITLIISSLIV
LIPSIFIVFISRILQGLSLGGEWGGASTVIIETYSSHRLRGTIASIIQLSVPIAIILSSSTLLLIFLYSIPWRESFLIIT
VLTLIALPLIRDVNKEKMFSSKIPLFEAILNDWKNILKSISIKVSESAAFYVFSAYIFSLYSNLKELPIIVLEAITFQLF
TIPLFGFLSDIIGRRLVVIIGLIIMGVGSYLLSLKIIYYGEIVISISDSALYAPQSSIFTEIFNKKYRFTAANFSYQLAS
ILGGALAPLLISDYSNLFIFIMLSYITITCIGVYFIEETKGKGIA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=383, Percent_Identity=27.6762402088773, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1790550, Length=399, Percent_Identity=24.0601503759398, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789941, Length=385, Percent_Identity=25.974025974026, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788942, Length=410, Percent_Identity=25.1219512195122, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 40634; Mature: 40634

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVIIGSLLLTISQWYAFFLFSQFSFIVFSEFIGLSIFIMGFISRALGSILFGYIGDKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRTALLLTGITLIISSLIVLIPSIFIVFISRILQGLSLGGEWGGASTVIIETYSSHRLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHH
GTIASIIQLSVPIAIILSSSTLLLIFLYSIPWRESFLIITVLTLIALPLIRDVNKEKMFS
HHHHHHHHHHCCHHEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
SKIPLFEAILNDWKNILKSISIKVSESAAFYVFSAYIFSLYSNLKELPIIVLEAITFQLF
HCCHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIPLFGFLSDIIGRRLVVIIGLIIMGVGSYLLSLKIIYYGEIVISISDSALYAPQSSIFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEECCHHHHHH
EIFNKKYRFTAANFSYQLASILGGALAPLLISDYSNLFIFIMLSYITITCIGVYFIEETK
HHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
GKGIA
CCCCC
>Mature Secondary Structure
MKRVIIGSLLLTISQWYAFFLFSQFSFIVFSEFIGLSIFIMGFISRALGSILFGYIGDKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRTALLLTGITLIISSLIVLIPSIFIVFISRILQGLSLGGEWGGASTVIIETYSSHRLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHH
GTIASIIQLSVPIAIILSSSTLLLIFLYSIPWRESFLIITVLTLIALPLIRDVNKEKMFS
HHHHHHHHHHCCHHEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
SKIPLFEAILNDWKNILKSISIKVSESAAFYVFSAYIFSLYSNLKELPIIVLEAITFQLF
HCCHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIPLFGFLSDIIGRRLVVIIGLIIMGVGSYLLSLKIIYYGEIVISISDSALYAPQSSIFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEECCHHHHHH
EIFNKKYRFTAANFSYQLASILGGALAPLLISDYSNLFIFIMLSYITITCIGVYFIEETK
HHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
GKGIA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]