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The map label for this gene is hisS

Identifier: 15920522

GI number: 15920522

Start: 344432

End: 345712

Strand: Direct

Name: hisS

Synonym: ST0323

Alternate gene names: 15920522

Gene position: 344432-345712 (Clockwise)

Preceding gene: 15920521

Following gene: 15920523

Centisome position: 12.78

GC content: 28.42

Gene sequence:

>1281_bases
ATGATTTCTTATGAGCCAGTAAGAGGAATGAAAGACTATTATGGCGAAGAGCTTCATAAAATAAAAGTAGTCGAGAATGC
TTTTTTGAAAATTGTAAAACTAGCTGGATATCAAGAAGTGGAAACACCTATTGTAGAAGATTTTCAACTATTCGCTTTAA
AAGGAGGAGAAGAACTTAGAAATACAATGTATGTCTTTAAAGATAAGGCCGGTAGAGAAGTTGCATTAAGACCCGAGTTC
ACACCTAGTATAGTTAGATTTTATCTTAATTCATTACAACATTTACCTAAGCCTATACGCTTATACTATTTAGGAACTGT
ATATAGATACGATGAGCCTCAATTTGGTAGATATAGGGAGTTTAGGCAAGCTGGTATAGAATTACTAGGATCTTCCAATA
TTTATTCAGATTTAGAAATATTACAAATATTAATAGAGATATATAGAGAATTAAATCTTATAAATAAAATTAGATTGAAA
ATAAATAATATATCATTAATAAGAAAAATACTTAATAAATTAAACATAAGTGATAATCTTCAAGAGCATTTTCTACATTT
AATAGATAAAGGAAAAATTGATGAGGCATTATCACTTTTACCAAGTTCAGAATATACAGAACTTATAACTAACATCCTAA
GTGTAAATAATTTAGATATCTCAAGTTATAATAAGATTAAAGAAGATCTTACAGAAAAATATAATTTAAAGGATTTAATT
CAAGATCTAGATAGGATTATGTTACTCAAAAATATATTAGATAATCTAGGTGTAAATTCATATATAGACTTAGGTTTTGT
TAGAGGTTTAGCTTATTATACCGGTTTAATATTTGAAGTGTTACATCCTTCTGTTTCTTTTAGTATTGCTGGTGGAGGAC
GATATGATAACCTTGTGGAACTGTATGGAGGTCCTCAAACTCCAGCTATAGGTTTTGCTATAGGAGTTGAGAGGACAGCA
TTAGTACTTGAGGAGCCTAATACTGTAAAAGAGAAGCAAAACAAAATAGGTGTAATAGTTTTATCAGACGAAGCTATATT
ATATGCTATTAGAATTGTGGATAAATTAAGATCAAATAATTATATTGCTACGATTAATCTAAAATCTATATCAATTTCTA
AATTAATTCCTTCTTATGCTGAGGAAGGGTACTCGTTCTTAATTTTCATAGGTAAAAAGGAATATGAAGATAAAACTATA
ACTTTGAAAAATCTCAGTACAAAAGAGCAAGTGACTATTAAGGAAGAAAACCTTTTGGACTATCTTAAGCAAATAATTTA
A

Upstream 100 bases:

>100_bases
AGAATTAAGAAAAAAGGTGTGATTGCAATAGTGGATAAATATGGTGATGTAACATACTATTTATTATCCGAAATAAATTT
AGGTAAAAAGTGAGTAAGAA

Downstream 100 bases:

>100_bases
GTTATCAAATAATCATTTTCATGGGTTATTAAAACATGGAAGAACTTCCTGCAACAGCGTTAGGTATTAAACTTAATGAC
GGTATAATTCTTGCTGCTGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MISYEPVRGMKDYYGEELHKIKVVENAFLKIVKLAGYQEVETPIVEDFQLFALKGGEELRNTMYVFKDKAGREVALRPEF
TPSIVRFYLNSLQHLPKPIRLYYLGTVYRYDEPQFGRYREFRQAGIELLGSSNIYSDLEILQILIEIYRELNLINKIRLK
INNISLIRKILNKLNISDNLQEHFLHLIDKGKIDEALSLLPSSEYTELITNILSVNNLDISSYNKIKEDLTEKYNLKDLI
QDLDRIMLLKNILDNLGVNSYIDLGFVRGLAYYTGLIFEVLHPSVSFSIAGGGRYDNLVELYGGPQTPAIGFAIGVERTA
LVLEEPNTVKEKQNKIGVIVLSDEAILYAIRIVDKLRSNNYIATINLKSISISKLIPSYAEEGYSFLIFIGKKEYEDKTI
TLKNLSTKEQVTIKEENLLDYLKQII

Sequences:

>Translated_426_residues
MISYEPVRGMKDYYGEELHKIKVVENAFLKIVKLAGYQEVETPIVEDFQLFALKGGEELRNTMYVFKDKAGREVALRPEF
TPSIVRFYLNSLQHLPKPIRLYYLGTVYRYDEPQFGRYREFRQAGIELLGSSNIYSDLEILQILIEIYRELNLINKIRLK
INNISLIRKILNKLNISDNLQEHFLHLIDKGKIDEALSLLPSSEYTELITNILSVNNLDISSYNKIKEDLTEKYNLKDLI
QDLDRIMLLKNILDNLGVNSYIDLGFVRGLAYYTGLIFEVLHPSVSFSIAGGGRYDNLVELYGGPQTPAIGFAIGVERTA
LVLEEPNTVKEKQNKIGVIVLSDEAILYAIRIVDKLRSNNYIATINLKSISISKLIPSYAEEGYSFLIFIGKKEYEDKTI
TLKNLSTKEQVTIKEENLLDYLKQII
>Mature_426_residues
MISYEPVRGMKDYYGEELHKIKVVENAFLKIVKLAGYQEVETPIVEDFQLFALKGGEELRNTMYVFKDKAGREVALRPEF
TPSIVRFYLNSLQHLPKPIRLYYLGTVYRYDEPQFGRYREFRQAGIELLGSSNIYSDLEILQILIEIYRELNLINKIRLK
INNISLIRKILNKLNISDNLQEHFLHLIDKGKIDEALSLLPSSEYTELITNILSVNNLDISSYNKIKEDLTEKYNLKDLI
QDLDRIMLLKNILDNLGVNSYIDLGFVRGLAYYTGLIFEVLHPSVSFSIAGGGRYDNLVELYGGPQTPAIGFAIGVERTA
LVLEEPNTVKEKQNKIGVIVLSDEAILYAIRIVDKLRSNNYIATINLKSISISKLIPSYAEEGYSFLIFIGKKEYEDKTI
TLKNLSTKEQVTIKEENLLDYLKQII

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=461, Percent_Identity=27.114967462039, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI15029520, Length=450, Percent_Identity=24.8888888888889, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1788861, Length=436, Percent_Identity=27.5229357798165, Blast_Score=158, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71993693, Length=459, Percent_Identity=25.0544662309368, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=459, Percent_Identity=25.0544662309368, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6325290, Length=369, Percent_Identity=26.2872628726287, Blast_Score=106, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24643061, Length=467, Percent_Identity=24.1970021413276, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24643059, Length=486, Percent_Identity=23.2510288065844, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_SULTO (Q975U9)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376191.1
- ProteinModelPortal:   Q975U9
- SMR:   Q975U9
- GeneID:   1458237
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0323
- NMPDR:   fig|273063.1.peg.359
- HOGENOM:   HBG616575
- OMA:   YYFANCF
- ProtClustDB:   PRK00037
- BioCyc:   STOK273063:ST0323-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48946; Mature: 48946

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISYEPVRGMKDYYGEELHKIKVVENAFLKIVKLAGYQEVETPIVEDFQLFALKGGEELR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHCEEEEEECCCHHHH
NTMYVFKDKAGREVALRPEFTPSIVRFYLNSLQHLPKPIRLYYLGTVYRYDEPQFGRYRE
HHHHHEECCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCHHHHH
FRQAGIELLGSSNIYSDLEILQILIEIYRELNLINKIRLKINNISLIRKILNKLNISDNL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHCCCCCCH
QEHFLHLIDKGKIDEALSLLPSSEYTELITNILSVNNLDISSYNKIKEDLTEKYNLKDLI
HHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHH
QDLDRIMLLKNILDNLGVNSYIDLGFVRGLAYYTGLIFEVLHPSVSFSIAGGGRYDNLVE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHE
LYGGPQTPAIGFAIGVERTALVLEEPNTVKEKQNKIGVIVLSDEAILYAIRIVDKLRSNN
ECCCCCCCCEEEEECCCCEEEEEECCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCC
YIATINLKSISISKLIPSYAEEGYSFLIFIGKKEYEDKTITLKNLSTKEQVTIKEENLLD
EEEEEEECCEEHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCCCEEEECHHHHHH
YLKQII
HHHHHC
>Mature Secondary Structure
MISYEPVRGMKDYYGEELHKIKVVENAFLKIVKLAGYQEVETPIVEDFQLFALKGGEELR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHCEEEEEECCCHHHH
NTMYVFKDKAGREVALRPEFTPSIVRFYLNSLQHLPKPIRLYYLGTVYRYDEPQFGRYRE
HHHHHEECCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCHHHHH
FRQAGIELLGSSNIYSDLEILQILIEIYRELNLINKIRLKINNISLIRKILNKLNISDNL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHCCCCCCH
QEHFLHLIDKGKIDEALSLLPSSEYTELITNILSVNNLDISSYNKIKEDLTEKYNLKDLI
HHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHH
QDLDRIMLLKNILDNLGVNSYIDLGFVRGLAYYTGLIFEVLHPSVSFSIAGGGRYDNLVE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHE
LYGGPQTPAIGFAIGVERTALVLEEPNTVKEKQNKIGVIVLSDEAILYAIRIVDKLRSNN
ECCCCCCCCEEEEECCCCEEEEEECCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCC
YIATINLKSISISKLIPSYAEEGYSFLIFIGKKEYEDKTITLKNLSTKEQVTIKEENLLD
EEEEEEECCEEHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCCCEEEECHHHHHH
YLKQII
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479