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The map label for this gene is gatB

Identifier: 15920474

GI number: 15920474

Start: 300361

End: 301800

Strand: Direct

Name: gatB

Synonym: ST0282

Alternate gene names: 15920474

Gene position: 300361-301800 (Clockwise)

Preceding gene: 15920473

Following gene: 15920475

Centisome position: 11.15

GC content: 33.82

Gene sequence:

>1440_bases
ATGCTTTCATCAATAAATATGGTTAAAATAGGACTAGAAGTACACGTTCATCTTACATCATTGAAAACTAAATTATTCTG
CTCCTGTCCTAGTGATTATACTGGGAAGGATCCTAATACAAACGTATGTCCCATATGCTTAGGTTTGCCTGGCGCAATTC
CAGTATTAAACGAAAACGCTGTAAAAGCAGCTATTATGGTTGCTCTAGCCATCAATGCCGAAATAGCTAATTCTTTGATT
ATGGTAAGAAAACACTATTTTTACCCCGATATGGCAAAGAACTACCAAATCTCCCAATATGATGGACCTGGTAGCATTGC
AATCAGTAAAGGTGGGTATCTAAAATTAAGAGAAGGCAAAACAGTTAGAATAAGAAGAATAAACATAGAAGAAGATCCTG
CGAAGATAATCTATCCTACGGGATCAATGCTAACTAGCAAATACACGTTACTAGATTATAATAGATCTGGAATGGGACTG
CTTGAAATAGTAACGGAACCTGATATGACGGAACCTAAAGAAGCGAGAGAATTTCTAGAGAAATTAAGATCTATATTAGA
ACACTTAGGTGTATGTAATTGTGATTTAGAAGGAGCTATGAGAGCAGACGCTAATGTCTCAGTAGAAGGAGGAGAGAGAG
TTGAGATAAAGAACATAGGTTCGCCCAGAGAAGTAGAAGAAGCGTTAAAATATGAAATTGCTAGACAAAAAGCTGCTATA
GCACAAGGTTTACCAGTTAAAAGAGAAACTAGGCATTGGGATTCTGAAAGAAAAGTAACAGTACCTACTAGGACAAAGGA
AACAGAAGAAGATTACAGATATTTCCCAGATCCGGATTTACCACCATATCCAATAACTCAGGACCTCATAGAAGAAATAA
GAAAGACTTTACCTGAATTACCCGATTTAAGGATAAAAAGATTAGTTACTCAGTATGGAATTTCAGATTACGATGCAACT
GTATTAGTTATGGATAAAGCATTAGCTGATTTATTTGAAGAGACTGCGAAACATTATAGTAATTATAAAAAATTAGTCAA
TTTATTAATAAATGATTATTTAAGATGGTTAAATGATAAAAACCTAAGGCCTTCCCAATCCAAGGCTGGAAGTCAGCATT
TAGTAGAATTATTAAAGTTGCTTGATGATGGAGTTATCACAATAAAAATAGCTAAGGAAATTCTTCCTGAAATTGTATTA
GAAGGAAAAATGCCATCATCTATAATAAAAGAGAGAGGCCTTGTTGCAATAAAAGATGAAGATTACTTAATTAATGTAAT
AAAAGAAGTAATTAATGAAGAACCAGATGCTGCTGAAAAGGCTAAGAATGACCCTAAGGTTATAAATTATCTAGTTGGTA
AAGTTATGAAAAAGACTGGAAAAAGAGCTGATCCACAATTAACTAACGAGTTAATAAAGAAGATTCTAGGCATTAAATAA

Upstream 100 bases:

>100_bases
ACAGTATCTATAGATCCAGAATTAGATAATAGAGGATATATTTTGCCCGGATTAGGTGATGCAGGAGATAGAGCATTTGG
TTAAACATTTTTATCCTTAA

Downstream 100 bases:

>100_bases
TCACAGCGTGGGTTTTTCATCACTTCTCAGCACTTAAAACCTCGATCATCAAAAATAATAGGAAGATGGTGATATTTATT
TGAATATGCAAAAAGACAAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MLSSINMVKIGLEVHVHLTSLKTKLFCSCPSDYTGKDPNTNVCPICLGLPGAIPVLNENAVKAAIMVALAINAEIANSLI
MVRKHYFYPDMAKNYQISQYDGPGSIAISKGGYLKLREGKTVRIRRINIEEDPAKIIYPTGSMLTSKYTLLDYNRSGMGL
LEIVTEPDMTEPKEAREFLEKLRSILEHLGVCNCDLEGAMRADANVSVEGGERVEIKNIGSPREVEEALKYEIARQKAAI
AQGLPVKRETRHWDSERKVTVPTRTKETEEDYRYFPDPDLPPYPITQDLIEEIRKTLPELPDLRIKRLVTQYGISDYDAT
VLVMDKALADLFEETAKHYSNYKKLVNLLINDYLRWLNDKNLRPSQSKAGSQHLVELLKLLDDGVITIKIAKEILPEIVL
EGKMPSSIIKERGLVAIKDEDYLINVIKEVINEEPDAAEKAKNDPKVINYLVGKVMKKTGKRADPQLTNELIKKILGIK

Sequences:

>Translated_479_residues
MLSSINMVKIGLEVHVHLTSLKTKLFCSCPSDYTGKDPNTNVCPICLGLPGAIPVLNENAVKAAIMVALAINAEIANSLI
MVRKHYFYPDMAKNYQISQYDGPGSIAISKGGYLKLREGKTVRIRRINIEEDPAKIIYPTGSMLTSKYTLLDYNRSGMGL
LEIVTEPDMTEPKEAREFLEKLRSILEHLGVCNCDLEGAMRADANVSVEGGERVEIKNIGSPREVEEALKYEIARQKAAI
AQGLPVKRETRHWDSERKVTVPTRTKETEEDYRYFPDPDLPPYPITQDLIEEIRKTLPELPDLRIKRLVTQYGISDYDAT
VLVMDKALADLFEETAKHYSNYKKLVNLLINDYLRWLNDKNLRPSQSKAGSQHLVELLKLLDDGVITIKIAKEILPEIVL
EGKMPSSIIKERGLVAIKDEDYLINVIKEVINEEPDAAEKAKNDPKVINYLVGKVMKKTGKRADPQLTNELIKKILGIK
>Mature_479_residues
MLSSINMVKIGLEVHVHLTSLKTKLFCSCPSDYTGKDPNTNVCPICLGLPGAIPVLNENAVKAAIMVALAINAEIANSLI
MVRKHYFYPDMAKNYQISQYDGPGSIAISKGGYLKLREGKTVRIRRINIEEDPAKIIYPTGSMLTSKYTLLDYNRSGMGL
LEIVTEPDMTEPKEAREFLEKLRSILEHLGVCNCDLEGAMRADANVSVEGGERVEIKNIGSPREVEEALKYEIARQKAAI
AQGLPVKRETRHWDSERKVTVPTRTKETEEDYRYFPDPDLPPYPITQDLIEEIRKTLPELPDLRIKRLVTQYGISDYDAT
VLVMDKALADLFEETAKHYSNYKKLVNLLINDYLRWLNDKNLRPSQSKAGSQHLVELLKLLDDGVITIKIAKEILPEIVL
EGKMPSSIIKERGLVAIKDEDYLINVIKEVINEEPDAAEKAKNDPKVINYLVGKVMKKTGKRADPQLTNELIKKILGIK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=36.0160965794769, Blast_Score=260, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI115534094, Length=301, Percent_Identity=40.531561461794, Blast_Score=185, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6319391, Length=524, Percent_Identity=30.5343511450382, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45550808, Length=493, Percent_Identity=32.2515212981744, Blast_Score=232, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_SULTO (Q975Z6)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376143.1
- ProteinModelPortal:   Q975Z6
- SMR:   Q975Z6
- GeneID:   1458186
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0282
- NMPDR:   fig|273063.1.peg.311
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   STOK273063:ST0282-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53943; Mature: 53943

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSSINMVKIGLEVHVHLTSLKTKLFCSCPSDYTGKDPNTNVCPICLGLPGAIPVLNENA
CCCCCCEEEEEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCHH
VKAAIMVALAINAEIANSLIMVRKHYFYPDMAKNYQISQYDGPGSIAISKGGYLKLREGK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCEEEEECCC
TVRIRRINIEEDPAKIIYPTGSMLTSKYTLLDYNRSGMGLLEIVTEPDMTEPKEAREFLE
EEEEEEECCCCCCCEEEECCCCHHHCEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHH
KLRSILEHLGVCNCDLEGAMRADANVSVEGGERVEIKNIGSPREVEEALKYEIARQKAAI
HHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
AQGLPVKRETRHWDSERKVTVPTRTKETEEDYRYFPDPDLPPYPITQDLIEEIRKTLPEL
HHCCCCCHHHHCCCCCCEEECCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PDLRIKRLVTQYGISDYDATVLVMDKALADLFEETAKHYSNYKKLVNLLINDYLRWLNDK
CCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLRPSQSKAGSQHLVELLKLLDDGVITIKIAKEILPEIVLEGKMPSSIIKERGLVAIKDE
CCCCCHHCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHCCEEEECCC
DYLINVIKEVINEEPDAAEKAKNDPKVINYLVGKVMKKTGKRADPQLTNELIKKILGIK
HHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSSINMVKIGLEVHVHLTSLKTKLFCSCPSDYTGKDPNTNVCPICLGLPGAIPVLNENA
CCCCCCEEEEEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCHH
VKAAIMVALAINAEIANSLIMVRKHYFYPDMAKNYQISQYDGPGSIAISKGGYLKLREGK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCEEEEECCC
TVRIRRINIEEDPAKIIYPTGSMLTSKYTLLDYNRSGMGLLEIVTEPDMTEPKEAREFLE
EEEEEEECCCCCCCEEEECCCCHHHCEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHH
KLRSILEHLGVCNCDLEGAMRADANVSVEGGERVEIKNIGSPREVEEALKYEIARQKAAI
HHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
AQGLPVKRETRHWDSERKVTVPTRTKETEEDYRYFPDPDLPPYPITQDLIEEIRKTLPEL
HHCCCCCHHHHCCCCCCEEECCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PDLRIKRLVTQYGISDYDATVLVMDKALADLFEETAKHYSNYKKLVNLLINDYLRWLNDK
CCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLRPSQSKAGSQHLVELLKLLDDGVITIKIAKEILPEIVLEGKMPSSIIKERGLVAIKDE
CCCCCHHCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHCCEEEECCC
DYLINVIKEVINEEPDAAEKAKNDPKVINYLVGKVMKKTGKRADPQLTNELIKKILGIK
HHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479