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The map label for this gene is hemB [H]

Identifier: 15920396

GI number: 15920396

Start: 236750

End: 237760

Strand: Direct

Name: hemB [H]

Synonym: ST0214

Alternate gene names: 15920396

Gene position: 236750-237760 (Clockwise)

Preceding gene: 15920395

Following gene: 15920397

Centisome position: 8.79

GC content: 35.31

Gene sequence:

>1011_bases
ATGGTAACATTTCCAACTCTAAGACCGAGGAGGCTAAGGAAAAATAAGTTAATAAGAGACTTAGTCGCAGAAACAAACTT
AACTGCTTCTGGGTTAATACAGCCTATTTTCATAAAAGAAAATATAAATGAACCAGAAAAGATAGATAGCATGCCTGGTG
TTTATAGATATCCACCTAATGATCACTTGATAAAGTTTGTCGAAGAAGTTTATAGTTTTGGCGTAAAGAATTTTATTCTC
TTCGGAATACCCTCATTTAAAGATGAATTTGCATCATCAGCATACGATAAAAATGGAATAATACAAAAATCACTAAGACT
GCTTAGGCAAACTTTCGGAGAGAAAATATTACTAATTACTGATGAATGTACAGATGAATATACCTCTCACGGACATTGTG
GAATTATTACACAAAAAGATGGAAAACTTTGCGTGAACAATGACGAAAGCCTAAAAGTTCATGCACTTATTGCAGTCAGC
CAAGCTGAGGCAGGAGCTGATATTATAGCACCTTCATCAATGATGGATGGTGTTGTTGGAGCTATTAGGAAGGCATTAGA
TGAAGCGGGTTTTACGGATACTGCAATAATGTCGTATAGTGTAAAATACGCATCAACGTTTTATTCACCTTTTAGAGATG
CAGCATATTCTAAACCAGCTTTTGGTGATAGAAGAGGATACCAAATGGATCCAAGAAACGCCTTTGAAGCATTGAAGGAG
GCTAGTTTAGATATAGAAGAAGGAGCTGATATTCTTATGGTGAAACCGGCTCACACTTATTTAGATGTTATAAGATTAGT
AAAGACAACCTTCCCACAATATCCATTAGCTGCGTATCATGTTAGTGGAGAATATAGTATGATTAAAGCAGCAGCCTTAA
ATGGATGGATAGATGAAAAAACAGCTGTTTTGGAAATAACTACTGCAATAAAGAGAGCTGGTGCAGATTTAATTATAACG
TATTATGCTAAAGACATAGCTAATTGGATAAAAGAAGGTGTACCTTTTTGA

Upstream 100 bases:

>100_bases
AATTCAATGCTTAAAAAAATCTTTTCACCGCTTTTTGAAACCGTTAGAAAAAATGAAGATATGGCAAATTACATTAACAA
CATTTTCGAAATATTAAGTA

Downstream 100 bases:

>100_bases
GCGAAAATCTTTGGAAGAAAGCCTTAGAATTATTTGCTGGAGGAGTAAATAGCCCTGTAAGAGCTGCCGTTAAACCATAC
CCCTTTTATGTTGAAAAAAG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MVTFPTLRPRRLRKNKLIRDLVAETNLTASGLIQPIFIKENINEPEKIDSMPGVYRYPPNDHLIKFVEEVYSFGVKNFIL
FGIPSFKDEFASSAYDKNGIIQKSLRLLRQTFGEKILLITDECTDEYTSHGHCGIITQKDGKLCVNNDESLKVHALIAVS
QAEAGADIIAPSSMMDGVVGAIRKALDEAGFTDTAIMSYSVKYASTFYSPFRDAAYSKPAFGDRRGYQMDPRNAFEALKE
ASLDIEEGADILMVKPAHTYLDVIRLVKTTFPQYPLAAYHVSGEYSMIKAAALNGWIDEKTAVLEITTAIKRAGADLIIT
YYAKDIANWIKEGVPF

Sequences:

>Translated_336_residues
MVTFPTLRPRRLRKNKLIRDLVAETNLTASGLIQPIFIKENINEPEKIDSMPGVYRYPPNDHLIKFVEEVYSFGVKNFIL
FGIPSFKDEFASSAYDKNGIIQKSLRLLRQTFGEKILLITDECTDEYTSHGHCGIITQKDGKLCVNNDESLKVHALIAVS
QAEAGADIIAPSSMMDGVVGAIRKALDEAGFTDTAIMSYSVKYASTFYSPFRDAAYSKPAFGDRRGYQMDPRNAFEALKE
ASLDIEEGADILMVKPAHTYLDVIRLVKTTFPQYPLAAYHVSGEYSMIKAAALNGWIDEKTAVLEITTAIKRAGADLIIT
YYAKDIANWIKEGVPF
>Mature_336_residues
MVTFPTLRPRRLRKNKLIRDLVAETNLTASGLIQPIFIKENINEPEKIDSMPGVYRYPPNDHLIKFVEEVYSFGVKNFIL
FGIPSFKDEFASSAYDKNGIIQKSLRLLRQTFGEKILLITDECTDEYTSHGHCGIITQKDGKLCVNNDESLKVHALIAVS
QAEAGADIIAPSSMMDGVVGAIRKALDEAGFTDTAIMSYSVKYASTFYSPFRDAAYSKPAFGDRRGYQMDPRNAFEALKE
ASLDIEEGADILMVKPAHTYLDVIRLVKTTFPQYPLAAYHVSGEYSMIKAAALNGWIDEKTAVLEITTAIKRAGADLIIT
YYAKDIANWIKEGVPF

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=316, Percent_Identity=45.8860759493671, Blast_Score=261, Evalue=6e-70,
Organism=Escherichia coli, GI87081728, Length=321, Percent_Identity=49.2211838006231, Blast_Score=293, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6321398, Length=323, Percent_Identity=39.3188854489164, Blast_Score=225, Evalue=7e-60,
Organism=Drosophila melanogaster, GI21358291, Length=293, Percent_Identity=43.3447098976109, Blast_Score=229, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37475; Mature: 37475

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTFPTLRPRRLRKNKLIRDLVAETNLTASGLIQPIFIKENINEPEKIDSMPGVYRYPPN
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCEEEEEEECCCCCCHHHCCCCCCEECCCC
DHLIKFVEEVYSFGVKNFILFGIPSFKDEFASSAYDKNGIIQKSLRLLRQTFGEKILLIT
HHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEE
DECTDEYTSHGHCGIITQKDGKLCVNNDESLKVHALIAVSQAEAGADIIAPSSMMDGVVG
CCCCHHHCCCCCEEEEEECCCCEEECCCCCEEEEEEEEEECCCCCCCEECCHHHHHHHHH
AIRKALDEAGFTDTAIMSYSVKYASTFYSPFRDAAYSKPAFGDRRGYQMDPRNAFEALKE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
ASLDIEEGADILMVKPAHTYLDVIRLVKTTFPQYPLAAYHVSGEYSMIKAAALNGWIDEK
CCCCHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCC
TAVLEITTAIKRAGADLIITYYAKDIANWIKEGVPF
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVTFPTLRPRRLRKNKLIRDLVAETNLTASGLIQPIFIKENINEPEKIDSMPGVYRYPPN
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCEEEEEEECCCCCCHHHCCCCCCEECCCC
DHLIKFVEEVYSFGVKNFILFGIPSFKDEFASSAYDKNGIIQKSLRLLRQTFGEKILLIT
HHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEE
DECTDEYTSHGHCGIITQKDGKLCVNNDESLKVHALIAVSQAEAGADIIAPSSMMDGVVG
CCCCHHHCCCCCEEEEEECCCCEEECCCCCEEEEEEEEEECCCCCCCEECCHHHHHHHHH
AIRKALDEAGFTDTAIMSYSVKYASTFYSPFRDAAYSKPAFGDRRGYQMDPRNAFEALKE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
ASLDIEEGADILMVKPAHTYLDVIRLVKTTFPQYPLAAYHVSGEYSMIKAAALNGWIDEK
CCCCHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCC
TAVLEITTAIKRAGADLIITYYAKDIANWIKEGVPF
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]