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The map label for this gene is thcD [H]

Identifier: 15920270

GI number: 15920270

Start: 114858

End: 115994

Strand: Direct

Name: thcD [H]

Synonym: ST0092

Alternate gene names: 15920270

Gene position: 114858-115994 (Clockwise)

Preceding gene: 15920268

Following gene: 15920271

Centisome position: 4.26

GC content: 31.84

Gene sequence:

>1137_bases
ATGGTAACTTCTGATAAATATTACCCATATGATAGACCTCCTTTATCAAAAGAATACCTCAGAGGGAAAATTGACAGAAA
CTCAATATTTTTTGAGCAACCAAACTATTACACAAAGAGAGAAAATATAAGGATTATACTGAATACGAGTGTTGAAAAAA
TTAATGTAAAAGAAAAAGGAGTTGAACTCAGTAATGGTACAATAATCCATTTTGATAAAGCTCTAATTGCAACTGGTGGT
AGACCAAGAAAGTTAAAATTACCGGGAGAAGAGTTAGAAGGAATCCATTACCTCAGAACGCTAGATGACTGTGACTCATT
AAAACAAGAAAATGGTAAAGAAGCTGTTATAATAGGCGGTGGCTTTATCGGTATAGAAGTCGCTTCTAGTCTTACAATGT
TAGGTGTAAAAACTACAGTAATAGAGGTTAAACCATATATTTGGAATACATTTGTAGATGAAAAAATTTCGAGACTAATA
CAAAATTATTTCGAAAAACATGGGGTTAATTTTATACTAAATGAGGGAGTTAAGGAGTTCATCGGAGATAAAAGAGTAAG
TAGAGTTATTACTGAAGGAGGCAAAACACTAAATGTAGACTTTGCATTAATAGCAGTAGGTATTGTTCCCAACATAGAAA
TAGCTCAGAAAAGTGGAATAGAAGTAGATAACGGAATTATAGCTAATGAATATCTTGAAACTTCAGCTAAAGATATTTAT
GTTGCCGGTGATGTAGCAAATATATATGACCCATCTATAGGTAAAAGGAGAAGGATAGAACATTGGAATAATGCAGAATA
TACCGGAAAATTAGCCGCTAAAAATATGTTAGGAAAGAGGGAGAAGTATAACTTCTTATCAACTATTTGGTCAGATATAT
TCGACTTACATATAGAGTCTGGTGGTGATACAACGGATTATGATGAGTACATAATTAGAGGCAACTTCTCTGTGGATAAT
CCTAATTTTAATGTAATATACTTGAAAGGGGGGGTTATAAAGGGTTATGTGGCTATAAATAGAGATTATGAAGAACTTTC
AGCATTAAATAAACTAATAGTTTCAAAGGTTGATGTTAGTAATAAGAAAAATGAGTTAAAGGACGAAAAATATGACCTAA
GTAAATTAAGTTTATAA

Upstream 100 bases:

>100_bases
AGTGTGAATACACTTGGAAAAGTTTACTGACTATTTAATAATTGGAAGTGGTGTTGCTGGATACCATGCACTAAAACAAT
TAGAAAATGAAAATGTAATC

Downstream 100 bases:

>100_bases
AGAATGTTTCGCTCTATTTTTTATGTATATTGAAGGATTACGTGCTGTTAATGGTGAAGGAGAATACATAAATGACCTAC
CAGATTTAGCTGGCACACTA

Product: reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MVTSDKYYPYDRPPLSKEYLRGKIDRNSIFFEQPNYYTKRENIRIILNTSVEKINVKEKGVELSNGTIIHFDKALIATGG
RPRKLKLPGEELEGIHYLRTLDDCDSLKQENGKEAVIIGGGFIGIEVASSLTMLGVKTTVIEVKPYIWNTFVDEKISRLI
QNYFEKHGVNFILNEGVKEFIGDKRVSRVITEGGKTLNVDFALIAVGIVPNIEIAQKSGIEVDNGIIANEYLETSAKDIY
VAGDVANIYDPSIGKRRRIEHWNNAEYTGKLAAKNMLGKREKYNFLSTIWSDIFDLHIESGGDTTDYDEYIIRGNFSVDN
PNFNVIYLKGGVIKGYVAINRDYEELSALNKLIVSKVDVSNKKNELKDEKYDLSKLSL

Sequences:

>Translated_378_residues
MVTSDKYYPYDRPPLSKEYLRGKIDRNSIFFEQPNYYTKRENIRIILNTSVEKINVKEKGVELSNGTIIHFDKALIATGG
RPRKLKLPGEELEGIHYLRTLDDCDSLKQENGKEAVIIGGGFIGIEVASSLTMLGVKTTVIEVKPYIWNTFVDEKISRLI
QNYFEKHGVNFILNEGVKEFIGDKRVSRVITEGGKTLNVDFALIAVGIVPNIEIAQKSGIEVDNGIIANEYLETSAKDIY
VAGDVANIYDPSIGKRRRIEHWNNAEYTGKLAAKNMLGKREKYNFLSTIWSDIFDLHIESGGDTTDYDEYIIRGNFSVDN
PNFNVIYLKGGVIKGYVAINRDYEELSALNKLIVSKVDVSNKKNELKDEKYDLSKLSL
>Mature_378_residues
MVTSDKYYPYDRPPLSKEYLRGKIDRNSIFFEQPNYYTKRENIRIILNTSVEKINVKEKGVELSNGTIIHFDKALIATGG
RPRKLKLPGEELEGIHYLRTLDDCDSLKQENGKEAVIIGGGFIGIEVASSLTMLGVKTTVIEVKPYIWNTFVDEKISRLI
QNYFEKHGVNFILNEGVKEFIGDKRVSRVITEGGKTLNVDFALIAVGIVPNIEIAQKSGIEVDNGIIANEYLETSAKDIY
VAGDVANIYDPSIGKRRRIEHWNNAEYTGKLAAKNMLGKREKYNFLSTIWSDIFDLHIESGGDTTDYDEYIIRGNFSVDN
PNFNVIYLKGGVIKGYVAINRDYEELSALNKLIVSKVDVSNKKNELKDEKYDLSKLSL

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=349, Percent_Identity=26.9340974212034, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI65787454, Length=349, Percent_Identity=26.9340974212034, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI226437568, Length=349, Percent_Identity=26.9340974212034, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI22202629, Length=328, Percent_Identity=32.6219512195122, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI4757732, Length=328, Percent_Identity=32.6219512195122, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI22202631, Length=168, Percent_Identity=31.547619047619, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI195927004, Length=103, Percent_Identity=39.8058252427184, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI195927006, Length=159, Percent_Identity=34.5911949685535, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI50301238, Length=206, Percent_Identity=33.495145631068, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788892, Length=349, Percent_Identity=28.6532951289398, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1789065, Length=312, Percent_Identity=22.4358974358974, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789765, Length=244, Percent_Identity=27.8688524590164, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559934, Length=304, Percent_Identity=32.8947368421053, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32564386, Length=317, Percent_Identity=28.7066246056782, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28573993, Length=335, Percent_Identity=30.4477611940298, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24581020, Length=335, Percent_Identity=30.4477611940298, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24639257, Length=323, Percent_Identity=27.8637770897833, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281359715, Length=323, Percent_Identity=27.8637770897833, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281359713, Length=323, Percent_Identity=27.8637770897833, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24639250, Length=323, Percent_Identity=27.8637770897833, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18543267, Length=323, Percent_Identity=27.8637770897833, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24639252, Length=323, Percent_Identity=27.8637770897833, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585130, Length=309, Percent_Identity=25.5663430420712, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42772; Mature: 42772

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSDKYYPYDRPPLSKEYLRGKIDRNSIFFEQPNYYTKRENIRIILNTSVEKINVKEKG
CCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEECCCCEECCCCEEEEEECCCEEEEHHHCC
VELSNGTIIHFDKALIATGGRPRKLKLPGEELEGIHYLRTLDDCDSLKQENGKEAVIIGG
EEECCCEEEEECCEEEECCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEECC
GFIGIEVASSLTMLGVKTTVIEVKPYIWNTFVDEKISRLIQNYFEKHGVNFILNEGVKEF
CEEEEHHHCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHH
IGDKRVSRVITEGGKTLNVDFALIAVGIVPNIEIAQKSGIEVDNGIIANEYLETSAKDIY
HCHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEHHCCCCCCCCCEEEHHHHHCCCCEEE
VAGDVANIYDPSIGKRRRIEHWNNAEYTGKLAAKNMLGKREKYNFLSTIWSDIFDLHIES
EECCCCHHCCCCCCCCHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECC
GGDTTDYDEYIIRGNFSVDNPNFNVIYLKGGVIKGYVAINRDYEELSALNKLIVSKVDVS
CCCCCCCCCEEEECCEEECCCCEEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHCCCC
NKKNELKDEKYDLSKLSL
CCHHHCCCHHCCHHHCCC
>Mature Secondary Structure
MVTSDKYYPYDRPPLSKEYLRGKIDRNSIFFEQPNYYTKRENIRIILNTSVEKINVKEKG
CCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEECCCCEECCCCEEEEEECCCEEEEHHHCC
VELSNGTIIHFDKALIATGGRPRKLKLPGEELEGIHYLRTLDDCDSLKQENGKEAVIIGG
EEECCCEEEEECCEEEECCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEECC
GFIGIEVASSLTMLGVKTTVIEVKPYIWNTFVDEKISRLIQNYFEKHGVNFILNEGVKEF
CEEEEHHHCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHH
IGDKRVSRVITEGGKTLNVDFALIAVGIVPNIEIAQKSGIEVDNGIIANEYLETSAKDIY
HCHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEHHCCCCCCCCCEEEHHHHHCCCCEEE
VAGDVANIYDPSIGKRRRIEHWNNAEYTGKLAAKNMLGKREKYNFLSTIWSDIFDLHIES
EECCCCHHCCCCCCCCHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECC
GGDTTDYDEYIIRGNFSVDNPNFNVIYLKGGVIKGYVAINRDYEELSALNKLIVSKVDVS
CCCCCCCCCEEEECCEEECCCCEEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHCCCC
NKKNELKDEKYDLSKLSL
CCHHHCCCHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]