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The map label for this gene is hisH [H]

Identifier: 159041969

GI number: 159041969

Start: 1481382

End: 1481999

Strand: Reverse

Name: hisH [H]

Synonym: Cmaq_1405

Alternate gene names: 159041969

Gene position: 1481999-1481382 (Counterclockwise)

Preceding gene: 159041970

Following gene: 159041968

Centisome position: 71.33

GC content: 46.6

Gene sequence:

>618_bases
ATGAGGGTGAGGGGAGTGGTTAAGGTTGGGGTTGTTAACTACGGTGTGGGTAATGTTGGAAGCATCTTCAATGCCCTACG
TAGGATTGAGGCTGAACCACTATTAATCAATAATACCGCTGACTTAAGGAGCGTGGACGCCGTAATACTCCCTGGGGTTG
GTTCCTTTAACTCAGCTATGGTTAATTTAAGTAGGTTAACTGATGAATTAAACAGGGTTAGGGGGAGTTCACCCATATTG
GGCATATGCCTAGGCCTACAGTTAATGTTCAAGGGTAGTGATGAGGGGGAGTTAAGGGGCTTGGGTTGGTATGATGGTTG
GGTTAATAGGATTAGGGGGCCAAGGGTGCCTCACATTGGTTGGGATTACGTTAAGTTAAGTGGGGATTGTAACCTAGGGG
TGGCTAGGGGTTACTACTACTTCATGCATAGTTACGCAGTGGTTAACCCGGTTAATGAACCCCCATTTGTCGGCTTCACT
AAGTACGGTGACTCAACAATACTGAGCGTACTCTGCGATGAGGGTAACGCCACCTACGGTACCCAATTCCACCCTGAGAA
GAGTGGGAAATTAGGCTTAAGTATTCTGAATGGCTTAGTTAATTTAGCTAGGAGGTGA

Upstream 100 bases:

>100_bases
GTTGTTAGGGAGTCAGCTGACCTAATGTATCATCTACTAGTCCTACTGAGGGTTATGGGGCTTAGTTTAAGCGACATATG
CAGGGAGTTGAGGAGGAGGC

Downstream 100 bases:

>100_bases
ATCATGGTTTCAATAATGTTTTAAAGCAGCCTTAATTAGGCTTCATTAATGCTGCCTAATGGTGTTAATGTACTTAAGTT
AAGCCCAGAGGAGGCTTCTA

Product: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MRVRGVVKVGVVNYGVGNVGSIFNALRRIEAEPLLINNTADLRSVDAVILPGVGSFNSAMVNLSRLTDELNRVRGSSPIL
GICLGLQLMFKGSDEGELRGLGWYDGWVNRIRGPRVPHIGWDYVKLSGDCNLGVARGYYYFMHSYAVVNPVNEPPFVGFT
KYGDSTILSVLCDEGNATYGTQFHPEKSGKLGLSILNGLVNLARR

Sequences:

>Translated_205_residues
MRVRGVVKVGVVNYGVGNVGSIFNALRRIEAEPLLINNTADLRSVDAVILPGVGSFNSAMVNLSRLTDELNRVRGSSPIL
GICLGLQLMFKGSDEGELRGLGWYDGWVNRIRGPRVPHIGWDYVKLSGDCNLGVARGYYYFMHSYAVVNPVNEPPFVGFT
KYGDSTILSVLCDEGNATYGTQFHPEKSGKLGLSILNGLVNLARR
>Mature_205_residues
MRVRGVVKVGVVNYGVGNVGSIFNALRRIEAEPLLINNTADLRSVDAVILPGVGSFNSAMVNLSRLTDELNRVRGSSPIL
GICLGLQLMFKGSDEGELRGLGWYDGWVNRIRGPRVPHIGWDYVKLSGDCNLGVARGYYYFMHSYAVVNPVNEPPFVGFT
KYGDSTILSVLCDEGNATYGTQFHPEKSGKLGLSILNGLVNLARR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=203, Percent_Identity=33.0049261083744, Blast_Score=91, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=32.0574162679426, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22364; Mature: 22364

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRGVVKVGVVNYGVGNVGSIFNALRRIEAEPLLINNTADLRSVDAVILPGVGSFNSAM
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCHHCCCEEEECCCCCCHHHH
VNLSRLTDELNRVRGSSPILGICLGLQLMFKGSDEGELRGLGWYDGWVNRIRGPRVPHIG
HHHHHHHHHHHHHCCCCCHHHHHHHEEEEECCCCCCCEEECCCHHHHHHHHCCCCCCCCC
WDYVKLSGDCNLGVARGYYYFMHSYAVVNPVNEPPFVGFTKYGDSTILSVLCDEGNATYG
CCEEEECCCCCCCHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCEEEEEEECCCCCCCC
TQFHPEKSGKLGLSILNGLVNLARR
CCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVRGVVKVGVVNYGVGNVGSIFNALRRIEAEPLLINNTADLRSVDAVILPGVGSFNSAM
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCHHCCCEEEECCCCCCHHHH
VNLSRLTDELNRVRGSSPILGICLGLQLMFKGSDEGELRGLGWYDGWVNRIRGPRVPHIG
HHHHHHHHHHHHHCCCCCHHHHHHHEEEEECCCCCCCEEECCCHHHHHHHHCCCCCCCCC
WDYVKLSGDCNLGVARGYYYFMHSYAVVNPVNEPPFVGFTKYGDSTILSVLCDEGNATYG
CCEEEECCCCCCCHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCEEEEEEECCCCCCCC
TQFHPEKSGKLGLSILNGLVNLARR
CCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]