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The map label for this gene is fabH [C]

Identifier: 159041805

GI number: 159041805

Start: 1310987

End: 1312036

Strand: Reverse

Name: fabH [C]

Synonym: Cmaq_1240

Alternate gene names: 159041805

Gene position: 1312036-1310987 (Counterclockwise)

Preceding gene: 159041806

Following gene: 159041804

Centisome position: 63.15

GC content: 47.9

Gene sequence:

>1050_bases
ATGGCGGTAGGCATAGTTGGTTGGGGTGCGTACATACCTAGGTATAGGATTAAGACCCGTGAAATAGCTGAGGCTTGGGG
TGATGATGCCCTCAGGATTAGGGACATGTACTTGGTTGAGGAGAAGGCGGTTGGGTACATTGACGAGGACCCAGTAACCA
TGGCTGTTGAAGCCTCTAGGGACGCTTTAATTAGGGCTGGGGTGAAGCCGAGTGAGGTTGGTGCAGTCTTCGCTGGAACT
GAGTCAAAACCCTACGCCGTTAAGCCGATATCATCAATACTCATTGATGCCCTAGGCCTAAATAGGCAGGTTTACTCAGT
GGACATGGAGTTCGCCTGTAAGGCTGGTTCAGATGCAATAATTAACCTAATGGGTTTAGTTAAGGCTAATTCAATAAAGT
ACGGTATAGCAGTGGGGACTGATTCATCCCAGGGTGAACCGGGGGAGCACTTGGAGTACACCGTGGGTACTGGCGCAGTC
GCATACGTGATTGGTTCAAGTAACCTGGCCGCTGAAATCAAGTACACTTACCCATATGCATCAGACACCCCTGACTTCTG
GAGGAGGGATAGTTCACCCTACCCAGTTCACGGTGAAGGCTTCACAGGGGAGCCGGCTTACTTCAAGCACATTATAGGTG
CTGCTAAGGGTTTAATGGATGAGTTAGGTATGAAGCCCAATGACTTCGATTACGCAGTCTTCCACCAACCCAACGCCAGG
TTCCCGGTTAGGGTTGCTCAAATGCTCGGCTTCCCGCTTGAGAAGGTTAAGCCAGGTATAGTGGTTGATTTAATAGGCAA
TACTTACAATGCCTCAGCATTACTGGGTTTAGCTAAGGTTCTTGAGGAGGCTAAGCCTGGTGCCAAAATAATAGTAGTCA
CCTTCGGTAGCGGTGCAGGCTCCAACGCATTCTACATTGAAACCACTGATCAATTACCAGGTAAGGTTAAGTTAGCTAGG
ACTATTGGTGAGATGCTTGAGGATAAGGTCTACATAGACTACTCCCTGTACTTAAAGTACAGGAAGATAATTAAAATGAT
ACACGGTTAA

Upstream 100 bases:

>100_bases
ATGCTGAGGTTGAGGTGGTGTTTAGGAGGGTTAAGGAGGATAGTGACTACGGTATAATTTACTACGGTTTTAAGTTAAGG
CCATTGAAGGGGTGTTGGTG

Downstream 100 bases:

>100_bases
TATTCATTAGGCTTGGCATATACCTAAAATTAAGCATTTTAATCACCCAGGTAATGCCAGGGGTACCTTATGTACGAAAC
ATTTATTAAGGGGGCTATTG

Product: hypothetical protein

Products: 3-oxoacyl-[acyl-carrier protein]; CO2; [acyl-carrier protein]

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MAVGIVGWGAYIPRYRIKTREIAEAWGDDALRIRDMYLVEEKAVGYIDEDPVTMAVEASRDALIRAGVKPSEVGAVFAGT
ESKPYAVKPISSILIDALGLNRQVYSVDMEFACKAGSDAIINLMGLVKANSIKYGIAVGTDSSQGEPGEHLEYTVGTGAV
AYVIGSSNLAAEIKYTYPYASDTPDFWRRDSSPYPVHGEGFTGEPAYFKHIIGAAKGLMDELGMKPNDFDYAVFHQPNAR
FPVRVAQMLGFPLEKVKPGIVVDLIGNTYNASALLGLAKVLEEAKPGAKIIVVTFGSGAGSNAFYIETTDQLPGKVKLAR
TIGEMLEDKVYIDYSLYLKYRKIIKMIHG

Sequences:

>Translated_349_residues
MAVGIVGWGAYIPRYRIKTREIAEAWGDDALRIRDMYLVEEKAVGYIDEDPVTMAVEASRDALIRAGVKPSEVGAVFAGT
ESKPYAVKPISSILIDALGLNRQVYSVDMEFACKAGSDAIINLMGLVKANSIKYGIAVGTDSSQGEPGEHLEYTVGTGAV
AYVIGSSNLAAEIKYTYPYASDTPDFWRRDSSPYPVHGEGFTGEPAYFKHIIGAAKGLMDELGMKPNDFDYAVFHQPNAR
FPVRVAQMLGFPLEKVKPGIVVDLIGNTYNASALLGLAKVLEEAKPGAKIIVVTFGSGAGSNAFYIETTDQLPGKVKLAR
TIGEMLEDKVYIDYSLYLKYRKIIKMIHG
>Mature_348_residues
AVGIVGWGAYIPRYRIKTREIAEAWGDDALRIRDMYLVEEKAVGYIDEDPVTMAVEASRDALIRAGVKPSEVGAVFAGTE
SKPYAVKPISSILIDALGLNRQVYSVDMEFACKAGSDAIINLMGLVKANSIKYGIAVGTDSSQGEPGEHLEYTVGTGAVA
YVIGSSNLAAEIKYTYPYASDTPDFWRRDSSPYPVHGEGFTGEPAYFKHIIGAAKGLMDELGMKPNDFDYAVFHQPNARF
PVRVAQMLGFPLEKVKPGIVVDLIGNTYNASALLGLAKVLEEAKPGAKIIVVTFGSGAGSNAFYIETTDQLPGKVKLART
IGEMLEDKVYIDYSLYLKYRKIIKMIHG

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Catalyzes The First Condensation Reaction Which Initiates Fatty Acid Synthesis And May Therefore Play A Role In Governing The

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0219 family

Homologues:

Organism=Escherichia coli, GI1787333, Length=324, Percent_Identity=24.6913580246914, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1240_CALMQ (A8ME61)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001541057.1
- ProteinModelPortal:   A8ME61
- SMR:   A8ME61
- GeneID:   5709542
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_1240
- HOGENOM:   HBG297330
- OMA:   ESKPYAV
- ProtClustDB:   PRK04262
- BioCyc:   CMAQ397948:CMAQ_1240-MONOMER
- HAMAP:   MF_01409
- InterPro:   IPR013747
- InterPro:   IPR004656
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038
- Gene3D:   G3DSA:3.40.47.10
- TIGRFAMs:   TIGR00748

Pfam domain/function: PF08541 ACP_syn_III_C; PF01154 HMG_CoA_synt_N; SSF53901 Thiolase-like

EC number: 2.3.1.41

Molecular weight: Translated: 37943; Mature: 37812

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGIVGWGAYIPRYRIKTREIAEAWGDDALRIRDMYLVEEKAVGYIDEDPVTMAVEASR
CEEEEEEHHHHCCCEEECHHHHHHHCCCCCEEEEEEEEEHHHCCCCCCCCCEEEEEECCC
DALIRAGVKPSEVGAVFAGTESKPYAVKPISSILIDALGLNRQVYSVDMEFACKAGSDAI
CHHEECCCCHHHCCEEEECCCCCCEEECHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHH
INLMGLVKANSIKYGIAVGTDSSQGEPGEHLEYTVGTGAVAYVIGSSNLAAEIKYTYPYA
HHHHHHHEECCEEEEEEECCCCCCCCCCCCEEEEECCCEEEEEEECCCEEEEEEEECCCC
SDTPDFWRRDSSPYPVHGEGFTGEPAYFKHIIGAAKGLMDELGMKPNDFDYAVFHQPNAR
CCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
FPVRVAQMLGFPLEKVKPGIVVDLIGNTYNASALLGLAKVLEEAKPGAKIIVVTFGSGAG
CHHHHHHHHCCCHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
SNAFYIETTDQLPGKVKLARTIGEMLEDKVYIDYSLYLKYRKIIKMIHG
CCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVGIVGWGAYIPRYRIKTREIAEAWGDDALRIRDMYLVEEKAVGYIDEDPVTMAVEASR
EEEEEEHHHHCCCEEECHHHHHHHCCCCCEEEEEEEEEHHHCCCCCCCCCEEEEEECCC
DALIRAGVKPSEVGAVFAGTESKPYAVKPISSILIDALGLNRQVYSVDMEFACKAGSDAI
CHHEECCCCHHHCCEEEECCCCCCEEECHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHH
INLMGLVKANSIKYGIAVGTDSSQGEPGEHLEYTVGTGAVAYVIGSSNLAAEIKYTYPYA
HHHHHHHEECCEEEEEEECCCCCCCCCCCCEEEEECCCEEEEEEECCCEEEEEEEECCCC
SDTPDFWRRDSSPYPVHGEGFTGEPAYFKHIIGAAKGLMDELGMKPNDFDYAVFHQPNAR
CCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
FPVRVAQMLGFPLEKVKPGIVVDLIGNTYNASALLGLAKVLEEAKPGAKIIVVTFGSGAG
CHHHHHHHHCCCHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
SNAFYIETTDQLPGKVKLARTIGEMLEDKVYIDYSLYLKYRKIIKMIHG
CCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acyl-[acyl-carrier protein]; malonyl-[acyl-carrier protein]

Specific reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA