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The map label for this gene is leuC

Identifier: 159041732

GI number: 159041732

Start: 1224914

End: 1226161

Strand: Reverse

Name: leuC

Synonym: Cmaq_1167

Alternate gene names: 159041732

Gene position: 1226161-1224914 (Counterclockwise)

Preceding gene: 159041733

Following gene: 159041730

Centisome position: 59.02

GC content: 49.6

Gene sequence:

>1248_bases
ATGGGCCTCACGTTAACCGAGAAGATACTGAGCAAGGCTGCGGGAAGGCAGGTTTCCCCAGGTGACGTAACGGAGATAAC
AGTTGACTTAGCGGCATTCCATGACCTAACCGGCCACCACGTAGTGGAGGTTATGGAGAATATTGGTGCCGTTAAGGTTT
GGGACCTTGATAGGTTCGTAATAGCGTTCGACCACTTGGCGCCGCCGCCTAATGATAGGGCTGCTGAGATTCAGGTTAAG
TTAAGGAAGTTCGCTAAATCCATTAACGTAAGGAACTTCCATGATGTTGGAGACGGCATCCTACATCAACTACTCCTAGA
GAAGTACGCGTTACCTGGTCAAGTAGTGATGGCTGCGGACAGCCACACAACAACAGTTGGCGCAGTGGGGGCATTCGCCC
AGGGAATGGGGGCCAGTGACATGGCTGCAATACTCATGACAGGTAAGACTTGGTTAATGATCCCTGAACCATTCCTAATA
AGGCTAATTAATGAACCAGCCCCAGGCGTATACGGTAAGGATGTGGCGCTTCACATACTCTCAGTGTTTAAGGCGGAGGG
CTTAAACGGTAAGTCTGTTGAATTACAGGTTGAGAAGCCTAAGGCATTCCCAATGGATTATAGGGCTACGGTATCAAACA
TGGGTGTTGAGTTCGGCGCCGATGCAGCAATATTTATTCCAGATGAGGAGACTGTAAGCTACCTGAGTAGAAGTAGGGGT
ATTAATGTTAAGCCAATTACCCCTGATCCAGACGCCAAGTACGTTGATGAGTACACCATTGAGTTAAATAAGCTTGAACC
ACTTGTGGCTGCACCGCATAGTGTTGATAACGTTAAGCCTGTTAGTGAGGTGGAGGGCATTGAGGTTGACTACGTCTTCA
TAGGGTCATGCACCAACGGTAGGTTAAGTGACCTTGAGGCTGCAGCCAGGATACTTAAGAATGGTAAGGTTAAGGCTAGG
TGCATTGTCATCCCAGCATCAAGGGACCTATTCACTAAGGCCCTTGACGCCGGTTACGTAGAGACGTTAACTAAGGCTGG
TTGTGTGGTTACGTACGGTACATGTGGACCATGCCTCGGAGGCCACTTCGGTGTAATTGGGCCAGGGGAGACTGCTGTGT
CTACTGGTAGTAGGAATTTTAAGGGTAGGATGGGGTCACCTGAGGGTAAGGTTTACCTAGCCAATGCAGCCACAGCGGCG
GCAACTGCGCTTGAGGGTAGGTTAACGGACCCGAGGAAGTACCTTTAA

Upstream 100 bases:

>100_bases
AATATTTAATGTTAGTTTTACAAATATTAAAACTAAGTTAAGTAGAATTCTTGGTAAAATTAATAAACCCACGATTAAAC
ACCCATACCAGTGATTAATA

Downstream 100 bases:

>100_bases
CCCTGAGCCTGGTTTAAACCCTCATCAGTGGCCTGGGTTTGATTATTACCCGTATCCCCCTGACCCTCGGCTTCACTTGA
TTCACTTGGCCTTGCTGCCT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MGLTLTEKILSKAAGRQVSPGDVTEITVDLAAFHDLTGHHVVEVMENIGAVKVWDLDRFVIAFDHLAPPPNDRAAEIQVK
LRKFAKSINVRNFHDVGDGILHQLLLEKYALPGQVVMAADSHTTTVGAVGAFAQGMGASDMAAILMTGKTWLMIPEPFLI
RLINEPAPGVYGKDVALHILSVFKAEGLNGKSVELQVEKPKAFPMDYRATVSNMGVEFGADAAIFIPDEETVSYLSRSRG
INVKPITPDPDAKYVDEYTIELNKLEPLVAAPHSVDNVKPVSEVEGIEVDYVFIGSCTNGRLSDLEAAARILKNGKVKAR
CIVIPASRDLFTKALDAGYVETLTKAGCVVTYGTCGPCLGGHFGVIGPGETAVSTGSRNFKGRMGSPEGKVYLANAATAA
ATALEGRLTDPRKYL

Sequences:

>Translated_415_residues
MGLTLTEKILSKAAGRQVSPGDVTEITVDLAAFHDLTGHHVVEVMENIGAVKVWDLDRFVIAFDHLAPPPNDRAAEIQVK
LRKFAKSINVRNFHDVGDGILHQLLLEKYALPGQVVMAADSHTTTVGAVGAFAQGMGASDMAAILMTGKTWLMIPEPFLI
RLINEPAPGVYGKDVALHILSVFKAEGLNGKSVELQVEKPKAFPMDYRATVSNMGVEFGADAAIFIPDEETVSYLSRSRG
INVKPITPDPDAKYVDEYTIELNKLEPLVAAPHSVDNVKPVSEVEGIEVDYVFIGSCTNGRLSDLEAAARILKNGKVKAR
CIVIPASRDLFTKALDAGYVETLTKAGCVVTYGTCGPCLGGHFGVIGPGETAVSTGSRNFKGRMGSPEGKVYLANAATAA
ATALEGRLTDPRKYL
>Mature_414_residues
GLTLTEKILSKAAGRQVSPGDVTEITVDLAAFHDLTGHHVVEVMENIGAVKVWDLDRFVIAFDHLAPPPNDRAAEIQVKL
RKFAKSINVRNFHDVGDGILHQLLLEKYALPGQVVMAADSHTTTVGAVGAFAQGMGASDMAAILMTGKTWLMIPEPFLIR
LINEPAPGVYGKDVALHILSVFKAEGLNGKSVELQVEKPKAFPMDYRATVSNMGVEFGADAAIFIPDEETVSYLSRSRGI
NVKPITPDPDAKYVDEYTIELNKLEPLVAAPHSVDNVKPVSEVEGIEVDYVFIGSCTNGRLSDLEAAARILKNGKVKARC
IVIPASRDLFTKALDAGYVETLTKAGCVVTYGTCGPCLGGHFGVIGPGETAVSTGSRNFKGRMGSPEGKVYLANAATAAA
TALEGRLTDPRKYL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=446, Percent_Identity=28.6995515695067, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI8659555, Length=409, Percent_Identity=23.4718826405868, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1786259, Length=361, Percent_Identity=31.3019390581717, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081781, Length=357, Percent_Identity=27.4509803921569, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI2367097, Length=151, Percent_Identity=35.0993377483444, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787531, Length=394, Percent_Identity=24.1116751269036, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=341, Percent_Identity=27.8592375366569, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI32564738, Length=341, Percent_Identity=27.8592375366569, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25149342, Length=293, Percent_Identity=26.2798634812287, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17568399, Length=152, Percent_Identity=29.6052631578947, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=31.7073170731707, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6323335, Length=366, Percent_Identity=30.0546448087432, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321429, Length=445, Percent_Identity=27.6404494382022, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=334, Percent_Identity=27.5449101796407, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28571643, Length=364, Percent_Identity=30.2197802197802, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI281365315, Length=446, Percent_Identity=26.6816143497758, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17864292, Length=446, Percent_Identity=26.6816143497758, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161076999, Length=365, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_CALMQ (A8MDY8)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001540984.1
- GeneID:   5709430
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_1167
- HOGENOM:   HBG330745
- OMA:   GADALMF
- ProtClustDB:   PRK00402
- BioCyc:   CMAQ397948:CMAQ_1167-MONOMER
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 44403; Mature: 44271

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTLTEKILSKAAGRQVSPGDVTEITVDLAAFHDLTGHHVVEVMENIGAVKVWDLDRFV
CCCCHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHCCCEEEEECCEEE
IAFDHLAPPPNDRAAEIQVKLRKFAKSINVRNFHDVGDGILHQLLLEKYALPGQVVMAAD
EEEHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
SHTTTVGAVGAFAQGMGASDMAAILMTGKTWLMIPEPFLIRLINEPAPGVYGKDVALHIL
CCCCCHHHHHHHHHCCCCCHHEEEEEECCEEEEECCHHHHHEECCCCCCCCCCHHHHHHH
SVFKAEGLNGKSVELQVEKPKAFPMDYRATVSNMGVEFGADAAIFIPDEETVSYLSRSRG
HHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHCCEEECCCEEEEECCHHHHHHHHHHCC
INVKPITPDPDAKYVDEYTIELNKLEPLVAAPHSVDNVKPVSEVEGIEVDYVFIGSCTNG
CEEECCCCCCCCCCCCEEEEEEECCCCHHCCCCCCCCCCCHHHCCCCEEEEEEEECCCCC
RLSDLEAAARILKNGKVKARCIVIPASRDLFTKALDAGYVETLTKAGCVVTYGTCGPCLG
CHHHHHHHHHHHHCCCEEEEEEEEECCHHHHHHHHCCHHHHHHHHCCCEEEECCCCCCCC
GHFGVIGPGETAVSTGSRNFKGRMGSPEGKVYLANAATAAATALEGRLTDPRKYL
CCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure 
GLTLTEKILSKAAGRQVSPGDVTEITVDLAAFHDLTGHHVVEVMENIGAVKVWDLDRFV
CCCHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHCCCEEEEECCEEE
IAFDHLAPPPNDRAAEIQVKLRKFAKSINVRNFHDVGDGILHQLLLEKYALPGQVVMAAD
EEEHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
SHTTTVGAVGAFAQGMGASDMAAILMTGKTWLMIPEPFLIRLINEPAPGVYGKDVALHIL
CCCCCHHHHHHHHHCCCCCHHEEEEEECCEEEEECCHHHHHEECCCCCCCCCCHHHHHHH
SVFKAEGLNGKSVELQVEKPKAFPMDYRATVSNMGVEFGADAAIFIPDEETVSYLSRSRG
HHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHCCEEECCCEEEEECCHHHHHHHHHHCC
INVKPITPDPDAKYVDEYTIELNKLEPLVAAPHSVDNVKPVSEVEGIEVDYVFIGSCTNG
CEEECCCCCCCCCCCCEEEEEEECCCCHHCCCCCCCCCCCHHHCCCCEEEEEEEECCCCC
RLSDLEAAARILKNGKVKARCIVIPASRDLFTKALDAGYVETLTKAGCVVTYGTCGPCLG
CHHHHHHHHHHHHCCCEEEEEEEEECCHHHHHHHHCCHHHHHHHHCCCEEEECCCCCCCC
GHFGVIGPGETAVSTGSRNFKGRMGSPEGKVYLANAATAAATALEGRLTDPRKYL
CCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA