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The map label for this gene is ogt [H]

Identifier: 159041635

GI number: 159041635

Start: 1119548

End: 1120126

Strand: Reverse

Name: ogt [H]

Synonym: Cmaq_1068

Alternate gene names: 159041635

Gene position: 1120126-1119548 (Counterclockwise)

Preceding gene: 159041640

Following gene: 159041633

Centisome position: 53.92

GC content: 43.35

Gene sequence:

>579_bases
GTGAAGAATAGCGATCAACTATGGAGGCTGGGTAAGAGTGACCTAGGGCTCTTGGTTCACAGGGATTTCAAGGGATTGGT
GGATAAGACCACGATAATTAGAATCAGTTCATTTCTACGCAAGTTCAGTAACATCAACATTACTGATTACGTTGAACCAG
ACCCATTAACTGTGGTTAGGATGGCTAAGATACCTAATGATAATTTAGGCCTAGTTATGCTTGCAATACTAGCCATACCG
AGGGGTTTCGTAGCCTCCTACAGTGACTTAGCTGAATTACTCAACATGACCCCACGCCTAATAGGCCTATTCGCCTCAAA
GAACCCTATGCCCGTCCTTATCCCATGCCATAGGGTTATTAACAGGGATGGCACAATAGGCGGGTACAGTGCGTGGCATA
GGGATGGTAAGGGTATTAAGGCGATGCTTTTAAGCATGGAGAACGTGAGGGTTAATGGTATTAAGGTTAGTAAGGATAAC
TTCGTGGACTCCAGTGAACTTAAGGATAACTTCTACAGAATATACAACTACTACCACTCATTAATGAGTGAGTACAGCTC
TAGCGAACAATCTGAATAA

Upstream 100 bases:

>100_bases
ATTACTACGCATCATTACTGTTTATAAACCATACTTTGGAGTAATTACCATGGTATCAGCATAGGTAACGGTTTTTAAGG
TTTAAAAATTGAATCAGTTC

Downstream 100 bases:

>100_bases
CATCACCCTCACTTAACCCATACTCACTGGAGAAACTCCCCCCATTAATCGCCAATTCTAGGAAACCCATACTATTAAAG
AGGGCGAGGGCCTCACCCTT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKNSDQLWRLGKSDLGLLVHRDFKGLVDKTTIIRISSFLRKFSNINITDYVEPDPLTVVRMAKIPNDNLGLVMLAILAIP
RGFVASYSDLAELLNMTPRLIGLFASKNPMPVLIPCHRVINRDGTIGGYSAWHRDGKGIKAMLLSMENVRVNGIKVSKDN
FVDSSELKDNFYRIYNYYHSLMSEYSSSEQSE

Sequences:

>Translated_192_residues
MKNSDQLWRLGKSDLGLLVHRDFKGLVDKTTIIRISSFLRKFSNINITDYVEPDPLTVVRMAKIPNDNLGLVMLAILAIP
RGFVASYSDLAELLNMTPRLIGLFASKNPMPVLIPCHRVINRDGTIGGYSAWHRDGKGIKAMLLSMENVRVNGIKVSKDN
FVDSSELKDNFYRIYNYYHSLMSEYSSSEQSE
>Mature_192_residues
MKNSDQLWRLGKSDLGLLVHRDFKGLVDKTTIIRISSFLRKFSNINITDYVEPDPLTVVRMAKIPNDNLGLVMLAILAIP
RGFVASYSDLAELLNMTPRLIGLFASKNPMPVLIPCHRVINRDGTIGGYSAWHRDGKGIKAMLLSMENVRVNGIKVSKDN
FVDSSELKDNFYRIYNYYHSLMSEYSSSEQSE

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 21746; Mature: 21746

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSDQLWRLGKSDLGLLVHRDFKGLVDKTTIIRISSFLRKFSNINITDYVEPDPLTVVR
CCCCHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEE
MAKIPNDNLGLVMLAILAIPRGFVASYSDLAELLNMTPRLIGLFASKNPMPVLIPCHRVI
EECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECHHHH
NRDGTIGGYSAWHRDGKGIKAMLLSMENVRVNGIKVSKDNFVDSSELKDNFYRIYNYYHS
CCCCCCCCCHHHHCCCCCHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LMSEYSSSEQSE
HHHHHCCCCCCC
>Mature Secondary Structure
MKNSDQLWRLGKSDLGLLVHRDFKGLVDKTTIIRISSFLRKFSNINITDYVEPDPLTVVR
CCCCHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEE
MAKIPNDNLGLVMLAILAIPRGFVASYSDLAELLNMTPRLIGLFASKNPMPVLIPCHRVI
EECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECHHHH
NRDGTIGGYSAWHRDGKGIKAMLLSMENVRVNGIKVSKDNFVDSSELKDNFYRIYNYYHS
CCCCCCCCCHHHHCCCCCHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LMSEYSSSEQSE
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]