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The map label for this gene is tpiA

Identifier: 159041277

GI number: 159041277

Start: 738920

End: 739597

Strand: Reverse

Name: tpiA

Synonym: Cmaq_0703

Alternate gene names: 159041277

Gene position: 739597-738920 (Counterclockwise)

Preceding gene: 159041278

Following gene: 159041275

Centisome position: 35.6

GC content: 47.05

Gene sequence:

>678_bases
ATGAAGGTTCCAGTACTCATAATAAACATGAAGGCTTACCCAGAGTTACTGGGGGGTGGTGCCGTTAAGCTGGCTCAAGT
AGCTGAGAAGGTTGCTAATAACCTGGGTGCATCCATAATAGTTGCACCACCGCACACTTACCTAAGGCAGGTTGCTGAAT
CCGTAAACATACCCGTTTACGCGCAGTCAGCTGACCCAGTGGATCCAGGGGCTAGGACTGGCCACATACCCTTAGAGTTC
ATTAAGGACGCTGGGGCTTCAGGTGTGATTATAAATCATAGTGAGCATAGGTTACTGTTAAATGACATAGCCATGCTTGT
TAATAAGGCCAAGGCCCTTAACCTTGAGACAGTGGTGTGTTCCCCAGACCCATTATCAAGTGCCGCTGCGGCTGCCCTTG
CACCCACGGCAGTTGCCATGGAGCCTCCTGAATTAATAGGCACAGGTAAGTCCGTTAGTAGGACTAAGCCTGATGTGGTT
GTTGAAACGGTTAATGCAGTTAAGAGGGTTAACGGTGATGTTAAGGTTATTACAGGTGCCGGTATAGAGGATTATAATGA
TGTTGCTAAGGCAATTGAATTAGGTACAGTGGGTGTATTGGTGGCTAGTGCAATAGTTAAGGCTAAGGATTGGGAGGCTA
AGATAACTGAATTAGCTAAACCCCTTGTGGGTAAGTGA

Upstream 100 bases:

>100_bases
TAAGGCTATTAAGGAGGCTGGTGGTGAAGTCTTAATAGTTCAATAGTGTTAATGATTAATATGAGCTAATGCTTATACAC
CCATTAACCTAGGCTTAATC

Downstream 100 bases:

>100_bases
GTATTCACTTTGAATACTCAATGATGCTTAAACTCAGGCTCATTATTAAGGTGAACGCTGAGCCCCCTCAATAACCCTTA
ACCTGCTTAATTGAAGATAA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MKVPVLIINMKAYPELLGGGAVKLAQVAEKVANNLGASIIVAPPHTYLRQVAESVNIPVYAQSADPVDPGARTGHIPLEF
IKDAGASGVIINHSEHRLLLNDIAMLVNKAKALNLETVVCSPDPLSSAAAAALAPTAVAMEPPELIGTGKSVSRTKPDVV
VETVNAVKRVNGDVKVITGAGIEDYNDVAKAIELGTVGVLVASAIVKAKDWEAKITELAKPLVGK

Sequences:

>Translated_225_residues
MKVPVLIINMKAYPELLGGGAVKLAQVAEKVANNLGASIIVAPPHTYLRQVAESVNIPVYAQSADPVDPGARTGHIPLEF
IKDAGASGVIINHSEHRLLLNDIAMLVNKAKALNLETVVCSPDPLSSAAAAALAPTAVAMEPPELIGTGKSVSRTKPDVV
VETVNAVKRVNGDVKVITGAGIEDYNDVAKAIELGTVGVLVASAIVKAKDWEAKITELAKPLVGK
>Mature_225_residues
MKVPVLIINMKAYPELLGGGAVKLAQVAEKVANNLGASIIVAPPHTYLRQVAESVNIPVYAQSADPVDPGARTGHIPLEF
IKDAGASGVIINHSEHRLLLNDIAMLVNKAKALNLETVVCSPDPLSSAAAAALAPTAVAMEPPELIGTGKSVSRTKPDVV
VETVNAVKRVNGDVKVITGAGIEDYNDVAKAIELGTVGVLVASAIVKAKDWEAKITELAKPLVGK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

None

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_CALMQ (A8MCN3)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001540529.1
- ProteinModelPortal:   A8MCN3
- SMR:   A8MCN3
- GeneID:   5709811
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_0703
- HOGENOM:   HBG297256
- OMA:   VEPPELI
- ProtClustDB:   PRK04302
- BioCyc:   CMAQ397948:CMAQ_0703-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_A
- InterPro:   IPR013785
- InterPro:   IPR000652
- InterPro:   IPR022891
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 23341; Mature: 23341

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 93-93 ACT_SITE 141-141 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVPVLIINMKAYPELLGGGAVKLAQVAEKVANNLGASIIVAPPHTYLRQVAESVNIPVY
CCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCEE
AQSADPVDPGARTGHIPLEFIKDAGASGVIINHSEHRLLLNDIAMLVNKAKALNLETVVC
ECCCCCCCCCCCCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEE
SPDPLSSAAAAALAPTAVAMEPPELIGTGKSVSRTKPDVVVETVNAVKRVNGDVKVITGA
CCCCCCHHHHHHHCCHHEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
GIEDYNDVAKAIELGTVGVLVASAIVKAKDWEAKITELAKPLVGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVPVLIINMKAYPELLGGGAVKLAQVAEKVANNLGASIIVAPPHTYLRQVAESVNIPVY
CCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCEE
AQSADPVDPGARTGHIPLEFIKDAGASGVIINHSEHRLLLNDIAMLVNKAKALNLETVVC
ECCCCCCCCCCCCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEE
SPDPLSSAAAAALAPTAVAMEPPELIGTGKSVSRTKPDVVVETVNAVKRVNGDVKVITGA
CCCCCCHHHHHHHCCHHEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
GIEDYNDVAKAIELGTVGVLVASAIVKAKDWEAKITELAKPLVGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA