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The map label for this gene is pyrI

Identifier: 159041147

GI number: 159041147

Start: 599231

End: 599722

Strand: Reverse

Name: pyrI

Synonym: Cmaq_0566

Alternate gene names: 159041147

Gene position: 599722-599231 (Counterclockwise)

Preceding gene: 159041148

Following gene: 159041146

Centisome position: 28.87

GC content: 41.67

Gene sequence:

>492_bases
ATGATGTCCAGTAAGGTTCAGGAGAGGGACTTAATAGTTAGTAAGATTAAGGATGGTATAGTTATTGATCATATACCGGC
TGGTAGAGCCTTAATAGTTCTTAGAGTGCTTAAACTAAAGGGTGGGGAGGGAAGGGTTGCCTTAGTTATGAATGCGGATA
GCCGTAGATTAGGGAGGAAGGACATAGTGAAGATTGAGGGTAAGGAGCTTAGTAATGATGAATTAAACCTAGTATCCCTA
ATAGCCCCAACGGCCACAATAAACATAATTAGGGATTACTCAGTTATTAAGAAGTTTCAAGTTAAGCTCCCTGAAACCGT
TAAGGGTGTTGTCAAGTGCAGGAACCCTAAGTGCATAACTAACCAGCCTAGGGAGGATGCTGTACCAACCTTCAGGGTGC
TTCACCTGGATCAACCCATTCTCCAATGCCAATACTGTGGAACATACTTAACGATTGATGATATTAATGCTCAATTAACT
GGTGAACGATGA

Upstream 100 bases:

>100_bases
AATCTCAGCATAATTAACCGCAGTGGTTGATTTAAATTAAATTAAAACATGTTTAAATGCCTTTAAACGAAAAATTAATT
AGCACCCACACCACTGAGTA

Downstream 100 bases:

>100_bases
ATGAGCATTGAGCTGGCTAGGTTATTGGTTAAGTACGGTGTGGTTAAGTTCGGTGACTTCACACTATCAAGTGGTGTTAA
GAGTAAGTACTACGTTGACA

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MMSSKVQERDLIVSKIKDGIVIDHIPAGRALIVLRVLKLKGGEGRVALVMNADSRRLGRKDIVKIEGKELSNDELNLVSL
IAPTATINIIRDYSVIKKFQVKLPETVKGVVKCRNPKCITNQPREDAVPTFRVLHLDQPILQCQYCGTYLTIDDINAQLT
GER

Sequences:

>Translated_163_residues
MMSSKVQERDLIVSKIKDGIVIDHIPAGRALIVLRVLKLKGGEGRVALVMNADSRRLGRKDIVKIEGKELSNDELNLVSL
IAPTATINIIRDYSVIKKFQVKLPETVKGVVKCRNPKCITNQPREDAVPTFRVLHLDQPILQCQYCGTYLTIDDINAQLT
GER
>Mature_163_residues
MMSSKVQERDLIVSKIKDGIVIDHIPAGRALIVLRVLKLKGGEGRVALVMNADSRRLGRKDIVKIEGKELSNDELNLVSL
IAPTATINIIRDYSVIKKFQVKLPETVKGVVKCRNPKCITNQPREDAVPTFRVLHLDQPILQCQYCGTYLTIDDINAQLT
GER

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=142, Percent_Identity=38.0281690140845, Blast_Score=94, Evalue=4e-21,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_CALMQ (A8MCA3)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001540399.1
- ProteinModelPortal:   A8MCA3
- SMR:   A8MCA3
- GeneID:   5709863
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_0566
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- ProtClustDB:   PRK00893
- BioCyc:   CMAQ397948:CMAQ_0566-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 18217; Mature: 18217

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSSKVQERDLIVSKIKDGIVIDHIPAGRALIVLRVLKLKGGEGRVALVMNADSRRLGRK
CCCCCCHHHHHHHHHHCCCEEEEECCCCCHHEEHHHHHCCCCCCCEEEEECCCCCCCCCC
DIVKIEGKELSNDELNLVSLIAPTATINIIRDYSVIKKFQVKLPETVKGVVKCRNPKCIT
CEEEECCCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
NQPREDAVPTFRVLHLDQPILQCQYCGTYLTIDDINAQLTGER
CCCCCCCCCEEEEEECCCHHHHHEECCEEEEEECCCEEEECCC
>Mature Secondary Structure
MMSSKVQERDLIVSKIKDGIVIDHIPAGRALIVLRVLKLKGGEGRVALVMNADSRRLGRK
CCCCCCHHHHHHHHHHCCCEEEEECCCCCHHEEHHHHHCCCCCCCEEEEECCCCCCCCCC
DIVKIEGKELSNDELNLVSLIAPTATINIIRDYSVIKKFQVKLPETVKGVVKCRNPKCIT
CEEEECCCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
NQPREDAVPTFRVLHLDQPILQCQYCGTYLTIDDINAQLTGER
CCCCCCCCCEEEEEECCCHHHHHEECCEEEEEECCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA