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The map label for this gene is ramA [H]

Identifier: 159041084

GI number: 159041084

Start: 537820

End: 538659

Strand: Reverse

Name: ramA [H]

Synonym: Cmaq_0501

Alternate gene names: 159041084

Gene position: 538659-537820 (Counterclockwise)

Preceding gene: 159041085

Following gene: 159041083

Centisome position: 25.93

GC content: 47.14

Gene sequence:

>840_bases
ATGCTGAGAGTACACTTAATTCAGTACGCCAGTAGGCTGGGTGATCCTGAGTATAATATAGATAGGTTAAGGAATTACGC
TAAAGCCAACTGCAGGGGTGATGGTAACGACGTCTTAATCACCCCTGAATTATATATCCCAGGCTACATGTCTAGGGACC
TCCTCCTCCAGATAGCTGAGCCGCTGGATGGTAAGTCAATAGGTGAGTTAACGGAGATAGCTAGGGAGGGTAAGTGCACC
ATAATCACTGGTATTGCTGAGAGGGATAAGGACACTGGGGTTGTTTATAATTCAGCAGTGGCTATTGGTGAGAACGGCTT
AATGGCACTCTACAGGAAGAGGCACCTACCCAGTTACGGTGTATTTGATGAGTCAAGGTACTTCGGGGTAGGTAGGGGTG
ATGCACCGGTCTTCTCAATGAATGGAACTAAGGCTGGGTTAGCAATATGTTACGACGCCTTCTACCCAGAGGTATCCAGG
TCACTAATGCTTAAGGGTGCTAGGGTTCAAGTATACATAAGTGCGGCCCCGGATATGTCAAGGCCTCACTTTGAGACATT
CATTAGGGCTAGGGCCATGGAGAACGTGTCCTTCGTAATCTACGTAAACACCATTGGTCAGTACGATGGTTTAGGCTTCT
TCGGGGGAAGCTTCATAGTGGATCCCCTGGGGGAGGTTGTGGCTAAGGCTAAGTACTATGAGGAGGACACTGTGGTTGCT
GAAATTGACCCAGGCCTAGTGGATAATTATAGGTCAATAAGACCAATACTGAAGGATTACACCTGGGATGACGTCAGGTA
TCTGCTTAAGAATTATAGGTCAGGCTATAGGGTGTATTAG

Upstream 100 bases:

>100_bases
AACGTAGGAGAACGCCCACTGGAGCCGGTGGAGGGGAGGGTGGCCAAGGTTAATCAACCATTGAATAACACTTATTAACT
TCCTCATTAAGCCTTCCTCA

Downstream 100 bases:

>100_bases
TGGTTAAATTAGTTAAAATGATTCGTGGAAAAACTATAAAAATGGTTAACCCACGGTGATTAGTGAAGTTAACTATGGCT
TTCATTTTTGAGCTACCTAA

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MLRVHLIQYASRLGDPEYNIDRLRNYAKANCRGDGNDVLITPELYIPGYMSRDLLLQIAEPLDGKSIGELTEIAREGKCT
IITGIAERDKDTGVVYNSAVAIGENGLMALYRKRHLPSYGVFDESRYFGVGRGDAPVFSMNGTKAGLAICYDAFYPEVSR
SLMLKGARVQVYISAAPDMSRPHFETFIRARAMENVSFVIYVNTIGQYDGLGFFGGSFIVDPLGEVVAKAKYYEEDTVVA
EIDPGLVDNYRSIRPILKDYTWDDVRYLLKNYRSGYRVY

Sequences:

>Translated_279_residues
MLRVHLIQYASRLGDPEYNIDRLRNYAKANCRGDGNDVLITPELYIPGYMSRDLLLQIAEPLDGKSIGELTEIAREGKCT
IITGIAERDKDTGVVYNSAVAIGENGLMALYRKRHLPSYGVFDESRYFGVGRGDAPVFSMNGTKAGLAICYDAFYPEVSR
SLMLKGARVQVYISAAPDMSRPHFETFIRARAMENVSFVIYVNTIGQYDGLGFFGGSFIVDPLGEVVAKAKYYEEDTVVA
EIDPGLVDNYRSIRPILKDYTWDDVRYLLKNYRSGYRVY
>Mature_279_residues
MLRVHLIQYASRLGDPEYNIDRLRNYAKANCRGDGNDVLITPELYIPGYMSRDLLLQIAEPLDGKSIGELTEIAREGKCT
IITGIAERDKDTGVVYNSAVAIGENGLMALYRKRHLPSYGVFDESRYFGVGRGDAPVFSMNGTKAGLAICYDAFYPEVSR
SLMLKGARVQVYISAAPDMSRPHFETFIRARAMENVSFVIYVNTIGQYDGLGFFGGSFIVDPLGEVVAKAKYYEEDTVVA
EIDPGLVDNYRSIRPILKDYTWDDVRYLLKNYRSGYRVY

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632350, Length=239, Percent_Identity=33.4728033472803, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI5031947, Length=239, Percent_Identity=33.4728033472803, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI297632348, Length=239, Percent_Identity=33.4728033472803, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI9910460, Length=236, Percent_Identity=30.0847457627119, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI297632346, Length=151, Percent_Identity=37.0860927152318, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1786412, Length=226, Percent_Identity=24.3362831858407, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556280, Length=231, Percent_Identity=28.1385281385281, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=202, Percent_Identity=28.2178217821782, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323383, Length=279, Percent_Identity=28.673835125448, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17933642, Length=217, Percent_Identity=30.8755760368664, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358471, Length=250, Percent_Identity=26, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355835, Length=206, Percent_Identity=29.6116504854369, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 31392; Mature: 31392

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVHLIQYASRLGDPEYNIDRLRNYAKANCRGDGNDVLITPELYIPGYMSRDLLLQIAE
CEEEHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCEECCCCCCHHHHHHHHC
PLDGKSIGELTEIAREGKCTIITGIAERDKDTGVVYNSAVAIGENGLMALYRKRHLPSYG
CCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCC
VFDESRYFGVGRGDAPVFSMNGTKAGLAICYDAFYPEVSRSLMLKGARVQVYISAAPDMS
CCCCCCEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
RPHFETFIRARAMENVSFVIYVNTIGQYDGLGFFGGSFIVDPLGEVVAKAKYYEEDTVVA
CHHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEE
EIDPGLVDNYRSIRPILKDYTWDDVRYLLKNYRSGYRVY
EECCCHHHCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLRVHLIQYASRLGDPEYNIDRLRNYAKANCRGDGNDVLITPELYIPGYMSRDLLLQIAE
CEEEHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCEECCCCCCHHHHHHHHC
PLDGKSIGELTEIAREGKCTIITGIAERDKDTGVVYNSAVAIGENGLMALYRKRHLPSYG
CCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCC
VFDESRYFGVGRGDAPVFSMNGTKAGLAICYDAFYPEVSRSLMLKGARVQVYISAAPDMS
CCCCCCEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
RPHFETFIRARAMENVSFVIYVNTIGQYDGLGFFGGSFIVDPLGEVVAKAKYYEEDTVVA
CHHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEE
EIDPGLVDNYRSIRPILKDYTWDDVRYLLKNYRSGYRVY
EECCCHHHCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA