Click here to switch to the map view.

The map label for this gene is infB

Identifier: 159041040

GI number: 159041040

Start: 495570

End: 497354

Strand: Reverse

Name: infB

Synonym: Cmaq_0457

Alternate gene names: 159041040

Gene position: 497354-495570 (Counterclockwise)

Preceding gene: 159041041

Following gene: 159041038

Centisome position: 23.94

GC content: 45.83

Gene sequence:

>1785_bases
ATGTCTGAGAATTATAGGGCACCCATAGTGGTTGTTGTTGGGCATGTGGACGTTGGGAAGACATTACTGTTGGATAAGAT
TAGGAACACCATGGTAGCCTACAGGGAGCCAGGTATGATTACCCAGCACATTGGCTTATCCTACTTGCCTTGGCCTATCA
TTGAGAAGTACGCTGCCCCAGTTATTGAGAGGTATAGGTTGAAGGGTAAGGTTTGGGTTAAGGGCTTCCTAATGGTTGAT
ACCCCAGGTCACGCAGCCTTCTCTAACCTTAGGCGTAGGGGTGGTTCAGTGGCTGACTTAGCCATACTTGTAATCGACCT
AACTAGGGGTTTTGAGGAGCAGACTTATGAGAGCCTAATACTGCTTAAGAGTAGGAATATACCCTTCGTTGTGGCGGCTA
ATAAGGTTGATAGGATATATGGGTGGAAGCCAATACCCAATGCCTCAATACTAGACTCCTATAATGCCCAGGATGAGGAG
ACCCAGGGTAGGCTTGAGGAGGCTTTAGCCAATATAATAATGGATTTTAATAAGCAGGGCTTTGAGGCTGAGAGGTTTGA
TAGGATAACTGACTTCAGTAGGCAAGTACCCATAGTGCCCACCAGTGCAGTGACAGGTGAGGGTATCCCGGATTTACTGG
TTCTAATGGCTGGTTTAACCCAAAGACTGGTTAAAGATAGGTTAAGGTTAGTTGGTGGTCCAGGTAAGGGTGTTGTAATG
GAGGTTAAGGAGGAGAAGGGGCTTGGAACCACAATGGATGTGGTCCTCTACGATGGTGTCATGAGGAAGGGAGACACCAT
AGTGGCCATGGGCCTTAACGGCCCTGTGGTTACTAGGATTAGAATGATGGTGATGCCTAAGCCGCTTGATGAAATGAGGG
ACCCGGAGGATAAGTACATGCATATTAATGAGGTTGAGGCAGCGGCTGGGGTTAAGGTGATTGCGGATGGGTTGGATGAT
GTTGTGCCAGGTTCATCAGTGTACGTGGTTCAGGGTGATCCTAAGCCGTACATTGATGAGGTTGTTAAGGATGCTGCATC
AGTTAAAATTGAAACTGATCAAATAGGTGTTGTGGCTAAGGCAGACACACTAGGTACGCTTGAGGCCATGGTGCTTTACC
TAAGGTCACAGGGAATACCTGTTAGGAAGGCTGATATTGGCCCAGTAACAAGGAGGGATATTATAGATGCCTCAGTGGTT
AGGAGGAAGAATCCACTATATGGTGTAGTACTGGCGTTTAACGTTAAGGTCCCTAAGGAGGTTGAGGAGGAGGCTAAGGT
TCAATTAGTTACAATATTCCAAAACAATATCCTTTACAGGCTTGTTGAGGAATTCACCAAGTGGTTTAACGAGGAGAAGA
GTAGGCTTATTGAGAGTGAGTTAAGTAAGTACGTTAGGCCAGGTAAAATAGCCATAATACCAGGCTACGTATTCAGGAGA
AGTGACCCAGCAATAGTGGGTGTTGAGGTGTTGGGTGGTTTAATTAAGCCAGGTTACAGGTTGGTTAAGGCTAATGGGAA
GGAGGTCGGCGTCATAATGCAGATTCAGGATAAGGGTAAGCCGATTCAAGTGGCTAAGAAGGGTATGAGTGTGGCGATTT
CAATAGAGGGTAATGTAATTGTTGGTAGGCACATTAAGGAGGGTGACGTACTCTACGTTAACGTACCATTGGAGCATGCT
GTTAAATTAATAATGCAGTATAAGGATCACTTAAGCAGTGATGAAGTTGAGGTACTTGAGGAATTCATGAAGCTTAGAAG
CACCTGGAAGGCTCAAGCCCAATGA

Upstream 100 bases:

>100_bases
TATGAATCCCATCAAGCTTTCAAGAACTCGAGCATTCCCCCTACCGAGTAACCTTTTGTGAAAAGCTTATTAAATAGGAT
TAACCCTTACTTCTGATACC

Downstream 100 bases:

>100_bases
GTACTCATTTACCTCTTGGCGTATAGCCAGCAGGCAACGTATATACCCTTCTTAACCTCAATCATGGGCGGCTCCTCCCT
ACGGCAAATATCCATGGCGA

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MSENYRAPIVVVVGHVDVGKTLLLDKIRNTMVAYREPGMITQHIGLSYLPWPIIEKYAAPVIERYRLKGKVWVKGFLMVD
TPGHAAFSNLRRRGGSVADLAILVIDLTRGFEEQTYESLILLKSRNIPFVVAANKVDRIYGWKPIPNASILDSYNAQDEE
TQGRLEEALANIIMDFNKQGFEAERFDRITDFSRQVPIVPTSAVTGEGIPDLLVLMAGLTQRLVKDRLRLVGGPGKGVVM
EVKEEKGLGTTMDVVLYDGVMRKGDTIVAMGLNGPVVTRIRMMVMPKPLDEMRDPEDKYMHINEVEAAAGVKVIADGLDD
VVPGSSVYVVQGDPKPYIDEVVKDAASVKIETDQIGVVAKADTLGTLEAMVLYLRSQGIPVRKADIGPVTRRDIIDASVV
RRKNPLYGVVLAFNVKVPKEVEEEAKVQLVTIFQNNILYRLVEEFTKWFNEEKSRLIESELSKYVRPGKIAIIPGYVFRR
SDPAIVGVEVLGGLIKPGYRLVKANGKEVGVIMQIQDKGKPIQVAKKGMSVAISIEGNVIVGRHIKEGDVLYVNVPLEHA
VKLIMQYKDHLSSDEVEVLEEFMKLRSTWKAQAQ

Sequences:

>Translated_594_residues
MSENYRAPIVVVVGHVDVGKTLLLDKIRNTMVAYREPGMITQHIGLSYLPWPIIEKYAAPVIERYRLKGKVWVKGFLMVD
TPGHAAFSNLRRRGGSVADLAILVIDLTRGFEEQTYESLILLKSRNIPFVVAANKVDRIYGWKPIPNASILDSYNAQDEE
TQGRLEEALANIIMDFNKQGFEAERFDRITDFSRQVPIVPTSAVTGEGIPDLLVLMAGLTQRLVKDRLRLVGGPGKGVVM
EVKEEKGLGTTMDVVLYDGVMRKGDTIVAMGLNGPVVTRIRMMVMPKPLDEMRDPEDKYMHINEVEAAAGVKVIADGLDD
VVPGSSVYVVQGDPKPYIDEVVKDAASVKIETDQIGVVAKADTLGTLEAMVLYLRSQGIPVRKADIGPVTRRDIIDASVV
RRKNPLYGVVLAFNVKVPKEVEEEAKVQLVTIFQNNILYRLVEEFTKWFNEEKSRLIESELSKYVRPGKIAIIPGYVFRR
SDPAIVGVEVLGGLIKPGYRLVKANGKEVGVIMQIQDKGKPIQVAKKGMSVAISIEGNVIVGRHIKEGDVLYVNVPLEHA
VKLIMQYKDHLSSDEVEVLEEFMKLRSTWKAQAQ
>Mature_593_residues
SENYRAPIVVVVGHVDVGKTLLLDKIRNTMVAYREPGMITQHIGLSYLPWPIIEKYAAPVIERYRLKGKVWVKGFLMVDT
PGHAAFSNLRRRGGSVADLAILVIDLTRGFEEQTYESLILLKSRNIPFVVAANKVDRIYGWKPIPNASILDSYNAQDEET
QGRLEEALANIIMDFNKQGFEAERFDRITDFSRQVPIVPTSAVTGEGIPDLLVLMAGLTQRLVKDRLRLVGGPGKGVVME
VKEEKGLGTTMDVVLYDGVMRKGDTIVAMGLNGPVVTRIRMMVMPKPLDEMRDPEDKYMHINEVEAAAGVKVIADGLDDV
VPGSSVYVVQGDPKPYIDEVVKDAASVKIETDQIGVVAKADTLGTLEAMVLYLRSQGIPVRKADIGPVTRRDIIDASVVR
RKNPLYGVVLAFNVKVPKEVEEEAKVQLVTIFQNNILYRLVEEFTKWFNEEKSRLIESELSKYVRPGKIAIIPGYVFRRS
DPAIVGVEVLGGLIKPGYRLVKANGKEVGVIMQIQDKGKPIQVAKKGMSVAISIEGNVIVGRHIKEGDVLYVNVPLEHAV
KLIMQYKDHLSSDEVEVLEEFMKLRSTWKAQAQ

Specific function: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI84043963, Length=595, Percent_Identity=37.3109243697479, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI53729339, Length=279, Percent_Identity=32.258064516129, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI53729337, Length=279, Percent_Identity=32.258064516129, Blast_Score=119, Evalue=6e-27,
Organism=Escherichia coli, GI1789559, Length=279, Percent_Identity=32.9749103942652, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI212656558, Length=582, Percent_Identity=36.7697594501718, Blast_Score=356, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI71994658, Length=278, Percent_Identity=29.8561151079137, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319282, Length=575, Percent_Identity=40.1739130434783, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324550, Length=575, Percent_Identity=23.4782608695652, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24656849, Length=603, Percent_Identity=36.9817578772803, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI28572034, Length=264, Percent_Identity=29.9242424242424, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2P_CALMQ (A8MBV9)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001540292.1
- ProteinModelPortal:   A8MBV9
- SMR:   A8MBV9
- GeneID:   5709932
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_0457
- HOGENOM:   HBG635083
- OMA:   SPLRIVP
- ProtClustDB:   PRK04004
- BioCyc:   CMAQ397948:CMAQ_0457-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00100_A
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004544
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00491
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66069; Mature: 65938

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENYRAPIVVVVGHVDVGKTLLLDKIRNTMVAYREPGMITQHIGLSYLPWPIIEKYAAP
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHEEECCCCCEEHHCCCCCCCHHHHHHHHHH
VIERYRLKGKVWVKGFLMVDTPGHAAFSNLRRRGGSVADLAILVIDLTRGFEEQTYESLI
HHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHCCCCHHHHHEEEEEHHCCCHHHHHHEEE
LLKSRNIPFVVAANKVDRIYGWKPIPNASILDSYNAQDEETQGRLEEALANIIMDFNKQG
EEECCCCCEEEECCCCHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
FEAERFDRITDFSRQVPIVPTSAVTGEGIPDLLVLMAGLTQRLVKDRLRLVGGPGKGVVM
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCEEE
EVKEEKGLGTTMDVVLYDGVMRKGDTIVAMGLNGPVVTRIRMMVMPKPLDEMRDPEDKYM
EEECCCCCCCEEHHHEEHHHHCCCCEEEEEECCCCHHHHEEEEECCCCHHHHCCCHHHEE
HINEVEAAAGVKVIADGLDDVVPGSSVYVVQGDPKPYIDEVVKDAASVKIETDQIGVVAK
EEEHHHHHCCHHHEECCHHHCCCCCEEEEEECCCCHHHHHHHHHHHCEEEECCCEEEEEE
ADTLGTLEAMVLYLRSQGIPVRKADIGPVTRRDIIDASVVRRKNPLYGVVLAFNVKVPKE
CCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHH
VEEEAKVQLVTIFQNNILYRLVEEFTKWFNEEKSRLIESELSKYVRPGKIAIIPGYVFRR
HHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEC
SDPAIVGVEVLGGLIKPGYRLVKANGKEVGVIMQIQDKGKPIQVAKKGMSVAISIEGNVI
CCCCEEEHHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEHEECCCCEEEEEEECCEE
VGRHIKEGDVLYVNVPLEHAVKLIMQYKDHLSSDEVEVLEEFMKLRSTWKAQAQ
EECEECCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SENYRAPIVVVVGHVDVGKTLLLDKIRNTMVAYREPGMITQHIGLSYLPWPIIEKYAAP
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHEEECCCCCEEHHCCCCCCCHHHHHHHHHH
VIERYRLKGKVWVKGFLMVDTPGHAAFSNLRRRGGSVADLAILVIDLTRGFEEQTYESLI
HHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHCCCCHHHHHEEEEEHHCCCHHHHHHEEE
LLKSRNIPFVVAANKVDRIYGWKPIPNASILDSYNAQDEETQGRLEEALANIIMDFNKQG
EEECCCCCEEEECCCCHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
FEAERFDRITDFSRQVPIVPTSAVTGEGIPDLLVLMAGLTQRLVKDRLRLVGGPGKGVVM
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCEEE
EVKEEKGLGTTMDVVLYDGVMRKGDTIVAMGLNGPVVTRIRMMVMPKPLDEMRDPEDKYM
EEECCCCCCCEEHHHEEHHHHCCCCEEEEEECCCCHHHHEEEEECCCCHHHHCCCHHHEE
HINEVEAAAGVKVIADGLDDVVPGSSVYVVQGDPKPYIDEVVKDAASVKIETDQIGVVAK
EEEHHHHHCCHHHEECCHHHCCCCCEEEEEECCCCHHHHHHHHHHHCEEEECCCEEEEEE
ADTLGTLEAMVLYLRSQGIPVRKADIGPVTRRDIIDASVVRRKNPLYGVVLAFNVKVPKE
CCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHH
VEEEAKVQLVTIFQNNILYRLVEEFTKWFNEEKSRLIESELSKYVRPGKIAIIPGYVFRR
HHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEC
SDPAIVGVEVLGGLIKPGYRLVKANGKEVGVIMQIQDKGKPIQVAKKGMSVAISIEGNVI
CCCCEEEHHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEHEECCCCEEEEEEECCEE
VGRHIKEGDVLYVNVPLEHAVKLIMQYKDHLSSDEVEVLEEFMKLRSTWKAQAQ
EECEECCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA