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The map label for this gene is speE [H]

Identifier: 159040967

GI number: 159040967

Start: 417934

End: 418809

Strand: Reverse

Name: speE [H]

Synonym: Cmaq_0383

Alternate gene names: 159040967

Gene position: 418809-417934 (Counterclockwise)

Preceding gene: 159040968

Following gene: 159040961

Centisome position: 20.16

GC content: 44.41

Gene sequence:

>876_bases
ATGAGTAATGGATACGTGATACACAACCCTCTCCAATGTGTTCAATACATTGCACCCCACACCTGGGTCTACATGAGTGT
TAGGAGGATTTTTGTTAATGAGAGGACTAAGTACCAGCATGTTCTCATAGTTGAGCTTGAGGACTTCGGTAAGGCACTCT
TCCTTGACGGTATCCTGCAGTCGGCTCAGGTTGATGAATACATTTACCATGAGTCACTGGTTCACCCAGCCATGGTGACT
GTTGGTAATCCTGAGAGGGTTCTGATACTGGGTACTGGTGAGGGTGCTGCCTTAAGGGAGGTTGTGAAGTATAGTAGTGT
TAAGGAGGTTGTTACAGTGGATATAGACTACGAGTTGATTAATTACGTTAAGGAGTACCTTAAGGAGTTCCACAATGACT
CATTCAAGGACCCGAGGGTTAAGGAGATTTACCAGGATGCCGTTGACTACGTTAGGAATACCGTAACCACTGGGGAGAAG
TTTGATGTGGTTATAATGGATTTAACGGACCCATACGGCTCGGAGATTGGTGCTAAGGTTTACTCAGTTGATGTGATTAG
GGGTATTTACAGTATGCTTAATGATAATGGGGCCTTGATCGTACAGGCTGGCTCAAGCTTCCTATTCCCTAGGGAATATG
GGCTTGTTTACGACACTGTTAAGTTAATATTCCCTCTAGTCACTGAGTACCAGGTTTGGGTACCATCATTCATGTACTCT
GAAAGCTTCATACTGGCGACCAAGGGGGTTAACCCAATTAAGTTAAGTAGCGATATTGTTGATGCAACCTTGAGGATTAA
TGGGGTTAAGACGAGGTTCTATAACGGTAAAGTGCACTCGGCGTTAATGGCCCTTGGTTCCTATAACCGTACTTAA

Upstream 100 bases:

>100_bases
GGGATGAGGGTATTAGTGGTTAACCATGGTTCAATAATAAGTGTAGGTTAATGAATGATGCTTAGGTATAGTAATAAACC
CACTGCTTCACCTTAAACCT

Downstream 100 bases:

>100_bases
CCTGCCTCCTAGGTAATTTTAAAAGAGGCCCTTAGCCTAATTTAACAGTGATGAGAGCCCTCGTGGCGGAGGAGTGAAGT
ATTCACGTACCGGCTGATTG

Product: spermine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSNGYVIHNPLQCVQYIAPHTWVYMSVRRIFVNERTKYQHVLIVELEDFGKALFLDGILQSAQVDEYIYHESLVHPAMVT
VGNPERVLILGTGEGAALREVVKYSSVKEVVTVDIDYELINYVKEYLKEFHNDSFKDPRVKEIYQDAVDYVRNTVTTGEK
FDVVIMDLTDPYGSEIGAKVYSVDVIRGIYSMLNDNGALIVQAGSSFLFPREYGLVYDTVKLIFPLVTEYQVWVPSFMYS
ESFILATKGVNPIKLSSDIVDATLRINGVKTRFYNGKVHSALMALGSYNRT

Sequences:

>Translated_291_residues
MSNGYVIHNPLQCVQYIAPHTWVYMSVRRIFVNERTKYQHVLIVELEDFGKALFLDGILQSAQVDEYIYHESLVHPAMVT
VGNPERVLILGTGEGAALREVVKYSSVKEVVTVDIDYELINYVKEYLKEFHNDSFKDPRVKEIYQDAVDYVRNTVTTGEK
FDVVIMDLTDPYGSEIGAKVYSVDVIRGIYSMLNDNGALIVQAGSSFLFPREYGLVYDTVKLIFPLVTEYQVWVPSFMYS
ESFILATKGVNPIKLSSDIVDATLRINGVKTRFYNGKVHSALMALGSYNRT
>Mature_290_residues
SNGYVIHNPLQCVQYIAPHTWVYMSVRRIFVNERTKYQHVLIVELEDFGKALFLDGILQSAQVDEYIYHESLVHPAMVTV
GNPERVLILGTGEGAALREVVKYSSVKEVVTVDIDYELINYVKEYLKEFHNDSFKDPRVKEIYQDAVDYVRNTVTTGEKF
DVVIMDLTDPYGSEIGAKVYSVDVIRGIYSMLNDNGALIVQAGSSFLFPREYGLVYDTVKLIFPLVTEYQVWVPSFMYSE
SFILATKGVNPIKLSSDIVDATLRINGVKTRFYNGKVHSALMALGSYNRT

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=275, Percent_Identity=25.4545454545455, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1786312, Length=266, Percent_Identity=33.4586466165414, Blast_Score=163, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25154106, Length=275, Percent_Identity=32, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI212645616, Length=223, Percent_Identity=32.2869955156951, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323175, Length=275, Percent_Identity=29.0909090909091, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6325326, Length=277, Percent_Identity=31.4079422382672, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24645443, Length=268, Percent_Identity=29.4776119402985, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21355941, Length=207, Percent_Identity=29.4685990338164, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21358269, Length=226, Percent_Identity=27.8761061946903, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24663238, Length=227, Percent_Identity=27.7533039647577, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 33061; Mature: 32930

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNGYVIHNPLQCVQYIAPHTWVYMSVRRIFVNERTKYQHVLIVELEDFGKALFLDGILQ
CCCCEEEECHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCHHHHHHHHH
SAQVDEYIYHESLVHPAMVTVGNPERVLILGTGEGAALREVVKYSSVKEVVTVDIDYELI
HHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHH
NYVKEYLKEFHNDSFKDPRVKEIYQDAVDYVRNTVTTGEKFDVVIMDLTDPYGSEIGAKV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCHH
YSVDVIRGIYSMLNDNGALIVQAGSSFLFPREYGLVYDTVKLIFPLVTEYQVWVPSFMYS
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCHHHHC
ESFILATKGVNPIKLSSDIVDATLRINGVKTRFYNGKVHSALMALGSYNRT
CCEEEEECCCCCEEECHHHEEEEEEEEEEEEEEECCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNGYVIHNPLQCVQYIAPHTWVYMSVRRIFVNERTKYQHVLIVELEDFGKALFLDGILQ
CCCEEEECHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCHHHHHHHHH
SAQVDEYIYHESLVHPAMVTVGNPERVLILGTGEGAALREVVKYSSVKEVVTVDIDYELI
HHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHH
NYVKEYLKEFHNDSFKDPRVKEIYQDAVDYVRNTVTTGEKFDVVIMDLTDPYGSEIGAKV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCHH
YSVDVIRGIYSMLNDNGALIVQAGSSFLFPREYGLVYDTVKLIFPLVTEYQVWVPSFMYS
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCHHHHC
ESFILATKGVNPIKLSSDIVDATLRINGVKTRFYNGKVHSALMALGSYNRT
CCEEEEECCCCCEEECHHHEEEEEEEEEEEEEEECCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA