Click here to switch to the map view.

The map label for this gene is pgl [H]

Identifier: 159040816

GI number: 159040816

Start: 264395

End: 265783

Strand: Reverse

Name: pgl [H]

Synonym: Cmaq_0230

Alternate gene names: 159040816

Gene position: 265783-264395 (Counterclockwise)

Preceding gene: 159040817

Following gene: 159040815

Centisome position: 12.79

GC content: 43.41

Gene sequence:

>1389_bases
ATGATTAATTCTCTTCCCTCAGGTAGGACGTATAATGTTGTTGAGTATGGTGCTGATCCTAAGGGTTTGGATGATAGTAC
TGGGGCTATAAATGAAGCTATTACCCAAGCCAGTGAGACTAGGGGTATTGTGTATATTCCTCCAGGCAACTACTTATCAA
GGAACATTATTCTGAGGAGTAATGTAATGTTACTCATTGATAAGGGTGCTGTGGTTAAATTCTCAACCGATTACAAGTCC
TATCCAATAATTGAGACTAGGAGAGAGGGGGTTCATCATTGTGGTGTTATGCCGTTAATATTCGGTAAGGATGTTAGGAA
TGTTAGGATTATTGGGGAGGGTGTGTTTGATGGCCAGGGTTACGCATGGTGGCCTATTAGGAGGTTTCGCGTTACTGAGG
ATTACTGGAGGAGGCTTGTTGAATCAGGGGGTGTTGTTGGTGATGATGGTAAAACCTGGTGGCCTACTAGGAATGCCATG
GAGGGTGCTGAGGCCTTCAGGAAAATAACCAGTGAAGGTGGGAAGCCGAGTACTGAGGATTGTGAGAGGTATAGGGAGTT
CTTTAGGCCTCAGCTTCTTCAACTCTATAATGCTGAGAACGTGACCATAGAAGGGGTTACGTTTAAGGACTCACCCATGT
GGACAATACACATTCTCTACTCAAGGCATGTTACATTAATAAACACTAGTAGTATTGCCCCAGATTACTCACCAAACACT
GATGGTGTTGTCGTGGATTCCTCAAGTGACGTTGAGGTAAGGGGCTGTATGATTGATGTTGGTGATGATTGCTTAGTCAT
AAAGTCTGGTAGGGATGAGGAGGGTAGGAGGATTGGCATACCCTCAGAGAATATTCACGCCTCAGGATGCTTAATGAAGA
GGGGGCATGGTGGATTCGTTATTGGTAGTGAAATGTCAGGTGGTGTTAGGAATGTTTCAATTCAGGATAGTGTATTCGAT
GGTACTGAGAGGGGTGTTAGGATTAAGACAACTAGGGGTAGGGGTGGTTTAATTGAGAATGTTTACGTAAACAACATCTA
CATGAGGAACATAATTCATGAGGCAGTGGTAGTGGATATGTTCTATGAGAAAAGGCCTGTTGAACCAGTATCAGAGAGGA
CGCCTAAGATTAGGGGTGTGGTTATTAGGAACACATCATGTGATGGGGCAGACCAGGCGGTGCTAATAAATGGGTTACCT
GAAATGCCCATTGAAGACATTATAATTGAGAATACTAGAATAACATCAAACAAGGGTATTCACATTGAAAACGCCTCAAG
TATTAGGCTCAGTAATGTTAAGGTGAACTCAAGGGCGATACCAGTCATAACCATGAGTAACGTGAGAAACATAACGTTAG
ACGACGTGAGCGGCTTATCCATGGAGTAA

Upstream 100 bases:

>100_bases
TTACCATTAATCATAACCTTAACCACCCCTAATTAATACCTACATGACGTACTGCTGGATAAGAAATGCTTTTAAATTGG
AACATTATTAATGCATTACC

Downstream 100 bases:

>100_bases
ATAGTTCAATAAGAGCAGGGTGATTGAGGATGCATGGAGTTGAATTTATTATACTTCACATTGAATTAAGGATATAATCA
ACAATGTTATGAGTAATTCA

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MINSLPSGRTYNVVEYGADPKGLDDSTGAINEAITQASETRGIVYIPPGNYLSRNIILRSNVMLLIDKGAVVKFSTDYKS
YPIIETRREGVHHCGVMPLIFGKDVRNVRIIGEGVFDGQGYAWWPIRRFRVTEDYWRRLVESGGVVGDDGKTWWPTRNAM
EGAEAFRKITSEGGKPSTEDCERYREFFRPQLLQLYNAENVTIEGVTFKDSPMWTIHILYSRHVTLINTSSIAPDYSPNT
DGVVVDSSSDVEVRGCMIDVGDDCLVIKSGRDEEGRRIGIPSENIHASGCLMKRGHGGFVIGSEMSGGVRNVSIQDSVFD
GTERGVRIKTTRGRGGLIENVYVNNIYMRNIIHEAVVVDMFYEKRPVEPVSERTPKIRGVVIRNTSCDGADQAVLINGLP
EMPIEDIIIENTRITSNKGIHIENASSIRLSNVKVNSRAIPVITMSNVRNITLDDVSGLSME

Sequences:

>Translated_462_residues
MINSLPSGRTYNVVEYGADPKGLDDSTGAINEAITQASETRGIVYIPPGNYLSRNIILRSNVMLLIDKGAVVKFSTDYKS
YPIIETRREGVHHCGVMPLIFGKDVRNVRIIGEGVFDGQGYAWWPIRRFRVTEDYWRRLVESGGVVGDDGKTWWPTRNAM
EGAEAFRKITSEGGKPSTEDCERYREFFRPQLLQLYNAENVTIEGVTFKDSPMWTIHILYSRHVTLINTSSIAPDYSPNT
DGVVVDSSSDVEVRGCMIDVGDDCLVIKSGRDEEGRRIGIPSENIHASGCLMKRGHGGFVIGSEMSGGVRNVSIQDSVFD
GTERGVRIKTTRGRGGLIENVYVNNIYMRNIIHEAVVVDMFYEKRPVEPVSERTPKIRGVVIRNTSCDGADQAVLINGLP
EMPIEDIIIENTRITSNKGIHIENASSIRLSNVKVNSRAIPVITMSNVRNITLDDVSGLSME
>Mature_462_residues
MINSLPSGRTYNVVEYGADPKGLDDSTGAINEAITQASETRGIVYIPPGNYLSRNIILRSNVMLLIDKGAVVKFSTDYKS
YPIIETRREGVHHCGVMPLIFGKDVRNVRIIGEGVFDGQGYAWWPIRRFRVTEDYWRRLVESGGVVGDDGKTWWPTRNAM
EGAEAFRKITSEGGKPSTEDCERYREFFRPQLLQLYNAENVTIEGVTFKDSPMWTIHILYSRHVTLINTSSIAPDYSPNT
DGVVVDSSSDVEVRGCMIDVGDDCLVIKSGRDEEGRRIGIPSENIHASGCLMKRGHGGFVIGSEMSGGVRNVSIQDSVFD
GTERGVRIKTTRGRGGLIENVYVNNIYMRNIIHEAVVVDMFYEKRPVEPVSERTPKIRGVVIRNTSCDGADQAVLINGLP
EMPIEDIIIENTRITSNKGIHIENASSIRLSNVKVNSRAIPVITMSNVRNITLDDVSGLSME

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 51338; Mature: 51338

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSLPSGRTYNVVEYGADPKGLDDSTGAINEAITQASETRGIVYIPPGNYLSRNIILRS
CCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEC
NVMLLIDKGAVVKFSTDYKSYPIIETRREGVHHCGVMPLIFGKDVRNVRIIGEGVFDGQG
CEEEEEECCEEEEEECCCCCCCCCHHHHCCHHHCCCEEEEECCCCCEEEEEEEEEECCCC
YAWWPIRRFRVTEDYWRRLVESGGVVGDDGKTWWPTRNAMEGAEAFRKITSEGGKPSTED
EEEECEEEEEECHHHHHHHHHCCCEECCCCCEECCCCHHHHHHHHHHHHHHCCCCCCHHH
CERYREFFRPQLLQLYNAENVTIEGVTFKDSPMWTIHILYSRHVTLINTSSIAPDYSPNT
HHHHHHHHCHHHHHHHCCCCEEEEEEEECCCCEEEEEEEEECEEEEEECCCCCCCCCCCC
DGVVVDSSSDVEVRGCMIDVGDDCLVIKSGRDEEGRRIGIPSENIHASGCLMKRGHGGFV
CCEEEECCCCEEEEEEEEEECCCEEEEECCCCCCCCEECCCCCCCCCCCEEEECCCCCEE
IGSEMSGGVRNVSIQDSVFDGTERGVRIKTTRGRGGLIENVYVNNIYMRNIIHEAVVVDM
EECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHH
FYEKRPVEPVSERTPKIRGVVIRNTSCDGADQAVLINGLPEMPIEDIIIENTRITSNKGI
HHCCCCCCCHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCCHHHEEEECEEEECCCCE
HIENASSIRLSNVKVNSRAIPVITMSNVRNITLDDVSGLSME
EEECCCCEEEEEEEECCEEEEEEEECCCCEEEECCCCCCCCC
>Mature Secondary Structure
MINSLPSGRTYNVVEYGADPKGLDDSTGAINEAITQASETRGIVYIPPGNYLSRNIILRS
CCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEC
NVMLLIDKGAVVKFSTDYKSYPIIETRREGVHHCGVMPLIFGKDVRNVRIIGEGVFDGQG
CEEEEEECCEEEEEECCCCCCCCCHHHHCCHHHCCCEEEEECCCCCEEEEEEEEEECCCC
YAWWPIRRFRVTEDYWRRLVESGGVVGDDGKTWWPTRNAMEGAEAFRKITSEGGKPSTED
EEEECEEEEEECHHHHHHHHHCCCEECCCCCEECCCCHHHHHHHHHHHHHHCCCCCCHHH
CERYREFFRPQLLQLYNAENVTIEGVTFKDSPMWTIHILYSRHVTLINTSSIAPDYSPNT
HHHHHHHHCHHHHHHHCCCCEEEEEEEECCCCEEEEEEEEECEEEEEECCCCCCCCCCCC
DGVVVDSSSDVEVRGCMIDVGDDCLVIKSGRDEEGRRIGIPSENIHASGCLMKRGHGGFV
CCEEEECCCCEEEEEEEEEECCCEEEEECCCCCCCCEECCCCCCCCCCCEEEECCCCCEE
IGSEMSGGVRNVSIQDSVFDGTERGVRIKTTRGRGGLIENVYVNNIYMRNIIHEAVVVDM
EECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHH
FYEKRPVEPVSERTPKIRGVVIRNTSCDGADQAVLINGLPEMPIEDIIIENTRITSNKGI
HHCCCCCCCHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCCHHHEEEECEEEECCCCE
HIENASSIRLSNVKVNSRAIPVITMSNVRNITLDDVSGLSME
EEECCCCEEEEEEEECCEEEEEEEECCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]