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The map label for this gene is pol-alpha [H]

Identifier: 159040732

GI number: 159040732

Start: 169477

End: 172092

Strand: Reverse

Name: pol-alpha [H]

Synonym: Cmaq_0144

Alternate gene names: 159040732

Gene position: 172092-169477 (Counterclockwise)

Preceding gene: 159040734

Following gene: 159040731

Centisome position: 8.28

GC content: 43.35

Gene sequence:

>2616_bases
GTGCCCAAGGATGAATTAGATAAAATTGAGGAACCAGAGGAAGAGGAGGTTGAGGAATCCAGTGAGGAGTATATTCAGGA
AAGCGCCATTACCGCTGAAACACCCACAAGCACACCCCCCAGTATATTAATTGACGTAACCTATGATGGTAAGGCCGGTA
AGGCCTTAGTGAAGCTTTACGACTTCATGAATGATAGGATCTACTTCTGGTACGATAACACTGGTCATAAACCATACTTA
ATAACCGATATACCACCCGATGAGTTGGTTACTAATTATAAGTCAATATTAAGGCTTAAGGGCTTCGATCACATTGATAC
AGTGGTTAAGTTCAATCCCCTTGAATTAAAGAGCAAAACCTACACGGTGGTTTACGCTAAGGATCCATTAACAATAGGTG
GTGGTAGGGAGTCCATTAGAGACATTATACCTAGGTCCTGGGAGGCTAGGATTAAGTATCACTTATCCTACATATATGAT
AATGGCCTAGTACCCGGTATGTTTTACAGGATTGAGAATGGTAAATTAACTCCAGTTAACGTTGATGTTCCAGGTGAGAT
TCAGAAGCTTGGGGAGGAGCTGTACGCTAATGATGAGGAGTACCTAAAGGCCTTCAGGGAGTGGTTACCCCTATTTCAAG
CACCTGTACCACCTTTGAAGAGGATTGCTATAGATATTGAGGTTTATACACCGCAGGAGAATAAGATACCTAACCCCAAG
GAGGCATTGTATGAGATAATTGCAATAGGCCTAGCTGGTAGTGATGGGCTTAAGAGGGTTCTGGTTCTTAGGAGGCCTGA
AATACTAATGAATGCTGATGACCAAGGCATGTTGCTGAGTGATGATGTTGAAGTCATGTTCTTCGATAATGAGAGGGATC
TCCTGAGGGAGTTCTTTAAAACAATAATGGCTTACCCAATATTAATCACCTTCAACGGTGACTCCTTTGACTTAACCTAC
ATTTACAATAGGGCACTTAAATTAGGCTTCAGGAAGGAGGAGTTACCCATAATCTTAACAAGGGATGGGGAGGCAAGGTA
CGTGCTTGGTGTTCACATCGACTTATACAAGTTCTTCAACATTAGGGCTGTTGAAGTCTATGCCTTCGGCGGCAAGTACT
CAGGCGGTGAAAAGACCCTGGACGCCATAGCAAGTGCATTACTCGGCATTTCCAAGGTTGAGAGGGAGAAGCCCATTAGT
GAAATGAATTACATTGAGTTAGTTAACTACAACTTCAGGGATGCGTTACTGACGCTTTACTTAACAACCTTCAATAATGA
ATTAGTCATGAGGTTAATAGTCCTATTAGCCAGGATATCGAAGACCCCGCTTAACGATGTTTCAAGGTACCAGGTCTCAG
CGTGGATAAGGAATATGGTTTACTTCGAGCATAGGAAGAGGGGTTGGTTAATACCTAATAAGGAGGATGTGGTGAATGCT
AAGGGTTCAGTGGCCACTAAGGCCATAATAAAGAATAAGAAGTATGCGGGTGCCATTGTGATAGAGCCCATTGCAGGCAT
ATACCCAAACGTTTACGTGCTTGACTTCGCAAGCCTATACCCATCAATAATAAAGAGGTGGAATCTATCCTATGAAACAG
TCAGGTGCCCTGACGAGAAGCAGGCCAATGATCCTAGGAATAGGCCAGTCCCAAACCTACCTCACTGGGTTTGTTCAAAT
ATGAGGGGTTTAACATCACTACTAGTTGGGTTACTTAGGGATATGAGGGTTTACGTGTATAAGAAGCTGGCTAAGGGTGA
TAAGGACCCGAGGCAGAGGGATTACTATAATGTTGTTCAAGCGGCGTTGAAGGTCTTCATAAACGCATCCTACGGCGTCT
TTGGAGCTGAAATATTCCCACTCTACTGCCCCCCATTGGCTGAACTAGTTACTGCCCTGGGTAGGTTGGCTATAACTAGG
ACTATAATTAAGGCCCTTGACTTAGGCCTGACCCCAATATACGGTGATACGGATAGTCTATTCCTCTATAATCCAAGTAA
GGATAAGCTTGAGGAGATGATAAATTGGGTTAAGGACGAGATGGGTATTGATATTGAGTTGGATAAGGCCTATAGGATAC
TTGCCTTAAGCGGTAGGAAGAAGAATTACGCAGGCATACTGCAGGATGGTTCAGTGGATATGAAGGGGCTTGTGGGTAAG
AAGAGGAATACACCTGATATAGCTAAGGATGCGGTTAAGGATGTTATACACCTCTTCAGTACAATAAGTAGTATTAATGA
TGTTGAGAAGGTTACTGAGGCTATTAAGGAGCGTGTGAGGGAGTACTACGTTATGATTAGGAATAAGGATGTACCATTGG
ATAAGCTTGCCGTTAAAATGGCTATAAATAAACCAATAAGCGAGTACACTAAGAACACACCGCAGCATGTTAAGGCCGCG
GAACTACTGGGTAAGTATGGGGTTAAGCCCGGTCCCGGTGACATAATATTCTTCGTTAAGACTACTACTAGGGAGGGGGT
TAAGCCTGTTCAGTTGGCTAGAATTGATGAGATTGACTCGGATAAGTACATTGAGTACTTAAGGACATCACTGGAGCAGA
TACTTGACGCCTTAGGCATACCCTTCGAGAACATTGTTGGGTCAAAGCTAATGTAG

Upstream 100 bases:

>100_bases
AACTCATCTCAATAGGTTAATTTTCCCCTTAAACTAAACTCACTTAATTAATAATTAACTTAAGCTGAAACCCTTATAAC
TTTCCAGTAACCGTAGACTT

Downstream 100 bases:

>100_bases
GAGACGCGTAATATTTAATAATTCACTTAAGGCATGTTAACCATGGAGTATGTATCTTTAGGCAAAACTGGGTTAAAGAT
ATCTATAATTGGTCTAGGTG

Product: DNA polymerase I

Products: NA

Alternate protein names: DNA polymerase B1; DNA polymerase I [H]

Number of amino acids: Translated: 871; Mature: 870

Protein sequence:

>871_residues
MPKDELDKIEEPEEEEVEESSEEYIQESAITAETPTSTPPSILIDVTYDGKAGKALVKLYDFMNDRIYFWYDNTGHKPYL
ITDIPPDELVTNYKSILRLKGFDHIDTVVKFNPLELKSKTYTVVYAKDPLTIGGGRESIRDIIPRSWEARIKYHLSYIYD
NGLVPGMFYRIENGKLTPVNVDVPGEIQKLGEELYANDEEYLKAFREWLPLFQAPVPPLKRIAIDIEVYTPQENKIPNPK
EALYEIIAIGLAGSDGLKRVLVLRRPEILMNADDQGMLLSDDVEVMFFDNERDLLREFFKTIMAYPILITFNGDSFDLTY
IYNRALKLGFRKEELPIILTRDGEARYVLGVHIDLYKFFNIRAVEVYAFGGKYSGGEKTLDAIASALLGISKVEREKPIS
EMNYIELVNYNFRDALLTLYLTTFNNELVMRLIVLLARISKTPLNDVSRYQVSAWIRNMVYFEHRKRGWLIPNKEDVVNA
KGSVATKAIIKNKKYAGAIVIEPIAGIYPNVYVLDFASLYPSIIKRWNLSYETVRCPDEKQANDPRNRPVPNLPHWVCSN
MRGLTSLLVGLLRDMRVYVYKKLAKGDKDPRQRDYYNVVQAALKVFINASYGVFGAEIFPLYCPPLAELVTALGRLAITR
TIIKALDLGLTPIYGDTDSLFLYNPSKDKLEEMINWVKDEMGIDIELDKAYRILALSGRKKNYAGILQDGSVDMKGLVGK
KRNTPDIAKDAVKDVIHLFSTISSINDVEKVTEAIKERVREYYVMIRNKDVPLDKLAVKMAINKPISEYTKNTPQHVKAA
ELLGKYGVKPGPGDIIFFVKTTTREGVKPVQLARIDEIDSDKYIEYLRTSLEQILDALGIPFENIVGSKLM

Sequences:

>Translated_871_residues
MPKDELDKIEEPEEEEVEESSEEYIQESAITAETPTSTPPSILIDVTYDGKAGKALVKLYDFMNDRIYFWYDNTGHKPYL
ITDIPPDELVTNYKSILRLKGFDHIDTVVKFNPLELKSKTYTVVYAKDPLTIGGGRESIRDIIPRSWEARIKYHLSYIYD
NGLVPGMFYRIENGKLTPVNVDVPGEIQKLGEELYANDEEYLKAFREWLPLFQAPVPPLKRIAIDIEVYTPQENKIPNPK
EALYEIIAIGLAGSDGLKRVLVLRRPEILMNADDQGMLLSDDVEVMFFDNERDLLREFFKTIMAYPILITFNGDSFDLTY
IYNRALKLGFRKEELPIILTRDGEARYVLGVHIDLYKFFNIRAVEVYAFGGKYSGGEKTLDAIASALLGISKVEREKPIS
EMNYIELVNYNFRDALLTLYLTTFNNELVMRLIVLLARISKTPLNDVSRYQVSAWIRNMVYFEHRKRGWLIPNKEDVVNA
KGSVATKAIIKNKKYAGAIVIEPIAGIYPNVYVLDFASLYPSIIKRWNLSYETVRCPDEKQANDPRNRPVPNLPHWVCSN
MRGLTSLLVGLLRDMRVYVYKKLAKGDKDPRQRDYYNVVQAALKVFINASYGVFGAEIFPLYCPPLAELVTALGRLAITR
TIIKALDLGLTPIYGDTDSLFLYNPSKDKLEEMINWVKDEMGIDIELDKAYRILALSGRKKNYAGILQDGSVDMKGLVGK
KRNTPDIAKDAVKDVIHLFSTISSINDVEKVTEAIKERVREYYVMIRNKDVPLDKLAVKMAINKPISEYTKNTPQHVKAA
ELLGKYGVKPGPGDIIFFVKTTTREGVKPVQLARIDEIDSDKYIEYLRTSLEQILDALGIPFENIVGSKLM
>Mature_870_residues
PKDELDKIEEPEEEEVEESSEEYIQESAITAETPTSTPPSILIDVTYDGKAGKALVKLYDFMNDRIYFWYDNTGHKPYLI
TDIPPDELVTNYKSILRLKGFDHIDTVVKFNPLELKSKTYTVVYAKDPLTIGGGRESIRDIIPRSWEARIKYHLSYIYDN
GLVPGMFYRIENGKLTPVNVDVPGEIQKLGEELYANDEEYLKAFREWLPLFQAPVPPLKRIAIDIEVYTPQENKIPNPKE
ALYEIIAIGLAGSDGLKRVLVLRRPEILMNADDQGMLLSDDVEVMFFDNERDLLREFFKTIMAYPILITFNGDSFDLTYI
YNRALKLGFRKEELPIILTRDGEARYVLGVHIDLYKFFNIRAVEVYAFGGKYSGGEKTLDAIASALLGISKVEREKPISE
MNYIELVNYNFRDALLTLYLTTFNNELVMRLIVLLARISKTPLNDVSRYQVSAWIRNMVYFEHRKRGWLIPNKEDVVNAK
GSVATKAIIKNKKYAGAIVIEPIAGIYPNVYVLDFASLYPSIIKRWNLSYETVRCPDEKQANDPRNRPVPNLPHWVCSNM
RGLTSLLVGLLRDMRVYVYKKLAKGDKDPRQRDYYNVVQAALKVFINASYGVFGAEIFPLYCPPLAELVTALGRLAITRT
IIKALDLGLTPIYGDTDSLFLYNPSKDKLEEMINWVKDEMGIDIELDKAYRILALSGRKKNYAGILQDGSVDMKGLVGKK
RNTPDIAKDAVKDVIHLFSTISSINDVEKVTEAIKERVREYYVMIRNKDVPLDKLAVKMAINKPISEYTKNTPQHVKAAE
LLGKYGVKPGPGDIIFFVKTTTREGVKPVQLARIDEIDSDKYIEYLRTSLEQILDALGIPFENIVGSKLM

Specific function: Thought To Be Involved In DNA Repair And/Or Mutagenesis. [C]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=674, Percent_Identity=25.9643916913947, Blast_Score=117, Evalue=5e-26,
Organism=Homo sapiens, GI106507301, Length=411, Percent_Identity=29.683698296837, Blast_Score=115, Evalue=2e-25,
Organism=Escherichia coli, GI1786246, Length=647, Percent_Identity=24.8840803709428, Blast_Score=104, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32565317, Length=432, Percent_Identity=26.3888888888889, Blast_Score=99, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17559488, Length=497, Percent_Identity=28.7726358148893, Blast_Score=96, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6324227, Length=400, Percent_Identity=28.5, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320101, Length=666, Percent_Identity=24.6246246246246, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648780, Length=469, Percent_Identity=25.5863539445629, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24664937, Length=510, Percent_Identity=26.2745098039216, Blast_Score=94, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 99346; Mature: 99215

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKDELDKIEEPEEEEVEESSEEYIQESAITAETPTSTPPSILIDVTYDGKAGKALVKLY
CCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHH
DFMNDRIYFWYDNTGHKPYLITDIPPDELVTNYKSILRLKGFDHIDTVVKFNPLELKSKT
HHHCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHEECCCEEECCCE
YTVVYAKDPLTIGGGRESIRDIIPRSWEARIKYHLSYIYDNGLVPGMFYRIENGKLTPVN
EEEEEECCCEEECCCHHHHHHHCCCCHHHEEEEEEEEEECCCCCCCEEEEECCCEEEEEE
VDVPGEIQKLGEELYANDEEYLKAFREWLPLFQAPVPPLKRIAIDIEVYTPQENKIPNPK
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCHH
EALYEIIAIGLAGSDGLKRVLVLRRPEILMNADDQGMLLSDDVEVMFFDNERDLLREFFK
HHHHHHHHHCCCCCCHHHHHHHEECCCCEECCCCCCCEEECCEEEEEECCCHHHHHHHHH
TIMAYPILITFNGDSFDLTYIYNRALKLGFRKEELPIILTRDGEARYVLGVHIDLYKFFN
HHHHCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEEEEEEEHHHC
IRAVEVYAFGGKYSGGEKTLDAIASALLGISKVEREKPISEMNYIELVNYNFRDALLTLY
EEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
LTTFNNELVMRLIVLLARISKTPLNDVSRYQVSAWIRNMVYFEHRKRGWLIPNKEDVVNA
HHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCC
KGSVATKAIIKNKKYAGAIVIEPIAGIYPNVYVLDFASLYPSIIKRWNLSYETVRCPDEK
CCCHHHHHHHCCCCCCCEEEECCHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCC
QANDPRNRPVPNLPHWVCSNMRGLTSLLVGLLRDMRVYVYKKLAKGDKDPRQRDYYNVVQ
CCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AALKVFINASYGVFGAEIFPLYCPPLAELVTALGRLAITRTIIKALDLGLTPIYGDTDSL
HHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
FLYNPSKDKLEEMINWVKDEMGIDIELDKAYRILALSGRKKNYAGILQDGSVDMKGLVGK
EEECCCHHHHHHHHHHHHHHHCCEEEECCCEEEEEECCCCCCCCEEEECCCCCHHHCCCC
KRNTPDIAKDAVKDVIHLFSTISSINDVEKVTEAIKERVREYYVMIRNKDVPLDKLAVKM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHH
AINKPISEYTKNTPQHVKAAELLGKYGVKPGPGDIIFFVKTTTREGVKPVQLARIDEIDS
HHCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCC
DKYIEYLRTSLEQILDALGIPFENIVGSKLM
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
>Mature Secondary Structure 
PKDELDKIEEPEEEEVEESSEEYIQESAITAETPTSTPPSILIDVTYDGKAGKALVKLY
CCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHH
DFMNDRIYFWYDNTGHKPYLITDIPPDELVTNYKSILRLKGFDHIDTVVKFNPLELKSKT
HHHCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHEECCCEEECCCE
YTVVYAKDPLTIGGGRESIRDIIPRSWEARIKYHLSYIYDNGLVPGMFYRIENGKLTPVN
EEEEEECCCEEECCCHHHHHHHCCCCHHHEEEEEEEEEECCCCCCCEEEEECCCEEEEEE
VDVPGEIQKLGEELYANDEEYLKAFREWLPLFQAPVPPLKRIAIDIEVYTPQENKIPNPK
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCHH
EALYEIIAIGLAGSDGLKRVLVLRRPEILMNADDQGMLLSDDVEVMFFDNERDLLREFFK
HHHHHHHHHCCCCCCHHHHHHHEECCCCEECCCCCCCEEECCEEEEEECCCHHHHHHHHH
TIMAYPILITFNGDSFDLTYIYNRALKLGFRKEELPIILTRDGEARYVLGVHIDLYKFFN
HHHHCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEEEEEEEHHHC
IRAVEVYAFGGKYSGGEKTLDAIASALLGISKVEREKPISEMNYIELVNYNFRDALLTLY
EEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
LTTFNNELVMRLIVLLARISKTPLNDVSRYQVSAWIRNMVYFEHRKRGWLIPNKEDVVNA
HHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCC
KGSVATKAIIKNKKYAGAIVIEPIAGIYPNVYVLDFASLYPSIIKRWNLSYETVRCPDEK
CCCHHHHHHHCCCCCCCEEEECCHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCC
QANDPRNRPVPNLPHWVCSNMRGLTSLLVGLLRDMRVYVYKKLAKGDKDPRQRDYYNVVQ
CCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AALKVFINASYGVFGAEIFPLYCPPLAELVTALGRLAITRTIIKALDLGLTPIYGDTDSL
HHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
FLYNPSKDKLEEMINWVKDEMGIDIELDKAYRILALSGRKKNYAGILQDGSVDMKGLVGK
EEECCCHHHHHHHHHHHHHHHCCEEEECCCEEEEEECCCCCCCCEEEECCCCCHHHCCCC
KRNTPDIAKDAVKDVIHLFSTISSINDVEKVTEAIKERVREYYVMIRNKDVPLDKLAVKM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHH
AINKPISEYTKNTPQHVKAAELLGKYGVKPGPGDIIFFVKTTTREGVKPVQLARIDEIDS
HHCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCC
DKYIEYLRTSLEQILDALGIPFENIVGSKLM
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10997874 [H]