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The map label for this gene is prpB [H]

Identifier: 15899316

GI number: 15899316

Start: 2355342

End: 2356199

Strand: Direct

Name: prpB [H]

Synonym: SSO2587

Alternate gene names: 15899316

Gene position: 2355342-2356199 (Clockwise)

Preceding gene: 15899315

Following gene: 15899317

Centisome position: 78.71

GC content: 36.48

Gene sequence:

>858_bases
TTGTCTCAAGTATTAAGGGAAGCTGATTTCTTAATGGTTCCGGGAGTTTTTAATCCTTTTACTGCAATTCTGGCAAAAAA
GGTAGGTTTTAAGGCTGTATACTTATCAGGTGCTGCTCTTACATCATCTTACGGCTTACCAGACATAGGTTTAATAACAT
TAGATGAAGTTGCTGAGATGATAAGAAGAATTAAAGAAGTTGTTGATATCCCGATAATAGTTGATTCAGATACCGGGTTT
GGTGAGGCAATGAACGTTTATAGAACTGTAAGAGTCCTAGAAAAAGCAGGTGCTGATGCTATTCAAATAGAGGATCAAAG
AATGCCAAAGAAATGTGGGCACTTAGAGGGTAAAGAGGTCGTGGAGCCTTTAGAAATGGTTCAAAAGATAAAGGCTGCTT
TAAAGGCTAGAAGAGATGCTTTAATAATAGCAAGAGTAGATTCTCGTGGTGTAATTGGTCTTGACGATGCAATAGAGAGG
GCTAAGATTTATTTAGAGGCAGGAGCTGATATAATATTTCCAGAGGCTTTAACCAGTAAGGAGGAATTCACTAAGTTCGC
TAAAGAGGTAAAAGCCCCGTTATTAGCTAATATGACTGAATTTGGTAAAACCCCTTACATTAAAGCTCAAGAGTTTAAGG
AGATGGGTTACAAATATGTAATATTCCCTGTTACTATATTTAGGGTAGCAGCTAAGGCGATGAAGGATGCCTTAGAGGTT
TTATTAAGAGAGGGAACACAAATCAACTTATTAGATAAAATGATTAATAGGCAACAGCAATATGAGATAATTGATTACTT
CTTCTACGAGAGATTAGACAAGGAATTAGGTAGTATAAAACTCGTTAGAAAGCTTTAA

Upstream 100 bases:

>100_bases
ATGACTGATGCTGAAGTTGAAGAAAAATACTTAAGGCTGAAAGGTAGAAAGGAAGAGTTAAATATTTTGTGGAATATGGA
AGAATTAAAGGTGAAGGAGA

Downstream 100 bases:

>100_bases
ATTTTTATATATGAAATTTAAATTATGGCAATAACTTCTAGAAGCTTAATTAAGAGATCTCCCGTGGTTGTTAAGGTAGG
TACTAAGGCTATTGATGCTT

Product: carboxyphosphonoenolpyruvate phosphonomutase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSQVLREADFLMVPGVFNPFTAILAKKVGFKAVYLSGAALTSSYGLPDIGLITLDEVAEMIRRIKEVVDIPIIVDSDTGF
GEAMNVYRTVRVLEKAGADAIQIEDQRMPKKCGHLEGKEVVEPLEMVQKIKAALKARRDALIIARVDSRGVIGLDDAIER
AKIYLEAGADIIFPEALTSKEEFTKFAKEVKAPLLANMTEFGKTPYIKAQEFKEMGYKYVIFPVTIFRVAAKAMKDALEV
LLREGTQINLLDKMINRQQQYEIIDYFFYERLDKELGSIKLVRKL

Sequences:

>Translated_285_residues
MSQVLREADFLMVPGVFNPFTAILAKKVGFKAVYLSGAALTSSYGLPDIGLITLDEVAEMIRRIKEVVDIPIIVDSDTGF
GEAMNVYRTVRVLEKAGADAIQIEDQRMPKKCGHLEGKEVVEPLEMVQKIKAALKARRDALIIARVDSRGVIGLDDAIER
AKIYLEAGADIIFPEALTSKEEFTKFAKEVKAPLLANMTEFGKTPYIKAQEFKEMGYKYVIFPVTIFRVAAKAMKDALEV
LLREGTQINLLDKMINRQQQYEIIDYFFYERLDKELGSIKLVRKL
>Mature_284_residues
SQVLREADFLMVPGVFNPFTAILAKKVGFKAVYLSGAALTSSYGLPDIGLITLDEVAEMIRRIKEVVDIPIIVDSDTGFG
EAMNVYRTVRVLEKAGADAIQIEDQRMPKKCGHLEGKEVVEPLEMVQKIKAALKARRDALIIARVDSRGVIGLDDAIERA
KIYLEAGADIIFPEALTSKEEFTKFAKEVKAPLLANMTEFGKTPYIKAQEFKEMGYKYVIFPVTIFRVAAKAMKDALEVL
LREGTQINLLDKMINRQQQYEIIDYFFYERLDKELGSIKLVRKL

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=269, Percent_Identity=44.6096654275093, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1790445, Length=279, Percent_Identity=26.5232974910394, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32091; Mature: 31960

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVLREADFLMVPGVFNPFTAILAKKVGFKAVYLSGAALTSSYGLPDIGLITLDEVAEM
CCHHHHHCCEEECCCCCHHHHHHHHHHCCCEEEEEECHHHHCCCCCCCCCEEEHHHHHHH
IRRIKEVVDIPIIVDSDTGFGEAMNVYRTVRVLEKAGADAIQIEDQRMPKKCGHLEGKEV
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHCCHHHCCCCCHHH
VEPLEMVQKIKAALKARRDALIIARVDSRGVIGLDDAIERAKIYLEAGADIIFPEALTSK
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHEECCCCEECCHHHCCH
EEFTKFAKEVKAPLLANMTEFGKTPYIKAQEFKEMGYKYVIFPVTIFRVAAKAMKDALEV
HHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
LLREGTQINLLDKMINRQQQYEIIDYFFYERLDKELGSIKLVRKL
HHHCCCEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure 
SQVLREADFLMVPGVFNPFTAILAKKVGFKAVYLSGAALTSSYGLPDIGLITLDEVAEM
CHHHHHCCEEECCCCCHHHHHHHHHHCCCEEEEEECHHHHCCCCCCCCCEEEHHHHHHH
IRRIKEVVDIPIIVDSDTGFGEAMNVYRTVRVLEKAGADAIQIEDQRMPKKCGHLEGKEV
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHCCHHHCCCCCHHH
VEPLEMVQKIKAALKARRDALIIARVDSRGVIGLDDAIERAKIYLEAGADIIFPEALTSK
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHEECCCCEECCHHHCCH
EEFTKFAKEVKAPLLANMTEFGKTPYIKAQEFKEMGYKYVIFPVTIFRVAAKAMKDALEV
HHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
LLREGTQINLLDKMINRQQQYEIIDYFFYERLDKELGSIKLVRKL
HHHCCCEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]