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The map label for this gene is pdhD-4 [H]

Identifier: 15899293

GI number: 15899293

Start: 2332633

End: 2333862

Strand: Direct

Name: pdhD-4 [H]

Synonym: SSO2559

Alternate gene names: 15899293

Gene position: 2332633-2333862 (Clockwise)

Preceding gene: 15899292

Following gene: 15899294

Centisome position: 77.96

GC content: 36.1

Gene sequence:

>1230_bases
ATGAAGATAGCAGTTATAGGTTCAGGTCCAGCTGGAGTATCAGCTTCATTAGAATTAGCCAAAAACAATAAGGTAGTATT
AGTGGAAAAAGAGGAAAGACTTGGAGGAACGTGCGTATTATATGGCTGTATTCCATCTAAGGCTATGCTACATCCCATAC
ACTTGGCTTCTGAACTCGAAAAGTTAGGCAAAAATATAACTTTCGATTTGGGAGAGCTGAGAAAATTAGGACAAGATGCG
TCATATAGGCTATCTAAGGGAGTCGAATATATGCTAGAAGATAATGGTGTAGAAGTTATTCATGGAATCGCATCTCTTAG
ATCTGGGCAATTAATAGTAAACAATGAGACTATCCAAGTGGATTACGTTGTTTTAGCTACTGGTACTTATAGAGAAGTTG
TTAAGGGTATAATATATTCAGAGGATTTGCCTTATTTGAACAAGGACTTTAAGAGCGTTATAATTGTTGGCGGAGATGTA
GGAGGTGTTGAATTTGGATGGATGTTAAGGAAACTTGGAAAGGAGGTAGTTCTAGTTGATAAGCAATCCTCGTTATTACC
ATATCTAGATAAGGAGGTTAGTAATGCTATAACTAACTACTTCTCTAAGATTGGTGTGAGATTGTATTTGAATCGTTCAG
TTAAGGAGATGAAAGAAAAAGAGGTGATATTAGAGAATGGAGAGAAGTTAACTGCGGACGTCGTATATATGACTTTTGGT
AGGAAGCCATCTATCCAAGGATTTGAGGAGATCAACCATAATCCTTTCGTCATCGTTGACGAATATTTAAGGACTAGTCT
AAGTAATGTTTTTGCTGCAGGTGATATAATTGGTACTCACACTGCCCATGAAGCAATGTACGCTGGGAAAATAGCTGCAA
CTAACATTATGGGTGGTAAAAAAGTATTTAATAAACAAGGCATACCTAAGGTCGTATATACTCACCCTACAATAGCTTAT
GTTGGAAGTATGGAAGGAAAATGCGTTAAAATAAATTTAGTTGAGTTAGGTCGTGCGATTACGGAAAAAGAGATTGATGG
ATTTCTTAAGCTATGTATTAAAGATGATAAGATTGTGGGAGCTCAAGCTTTTATGAAGGATGCTGAAGAAGTGATATCAC
TAATTTCATTCTTAATTAGGTATAATATTAAGGTTAATGAGATTAAGGATTATGTAGCTCCTCATCCTTCCTATATCGAA
GCTTTAACTGAACTTCTAAATAGACTCTGA

Upstream 100 bases:

>100_bases
CTTCATTATAGCCATAACTGGACTTATTGCGGGACCGGAAACTTATAAGACAAGTCTAGAAGAGTGATTTGTTTTTTAAT
GTTAAGTCAAATATTTTCTT

Downstream 100 bases:

>100_bases
GATAGATTCATTCAAATTTCATTGCACAGCACAGTAAATAAGCATATATAGCTGGATAGCCTATCCATAAATATGGATGT
ATACGAAGCAATACGAAAGA

Product: dihydrolipoamide dehydrogenase (pdhD-4)

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKIAVIGSGPAGVSASLELAKNNKVVLVEKEERLGGTCVLYGCIPSKAMLHPIHLASELEKLGKNITFDLGELRKLGQDA
SYRLSKGVEYMLEDNGVEVIHGIASLRSGQLIVNNETIQVDYVVLATGTYREVVKGIIYSEDLPYLNKDFKSVIIVGGDV
GGVEFGWMLRKLGKEVVLVDKQSSLLPYLDKEVSNAITNYFSKIGVRLYLNRSVKEMKEKEVILENGEKLTADVVYMTFG
RKPSIQGFEEINHNPFVIVDEYLRTSLSNVFAAGDIIGTHTAHEAMYAGKIAATNIMGGKKVFNKQGIPKVVYTHPTIAY
VGSMEGKCVKINLVELGRAITEKEIDGFLKLCIKDDKIVGAQAFMKDAEEVISLISFLIRYNIKVNEIKDYVAPHPSYIE
ALTELLNRL

Sequences:

>Translated_409_residues
MKIAVIGSGPAGVSASLELAKNNKVVLVEKEERLGGTCVLYGCIPSKAMLHPIHLASELEKLGKNITFDLGELRKLGQDA
SYRLSKGVEYMLEDNGVEVIHGIASLRSGQLIVNNETIQVDYVVLATGTYREVVKGIIYSEDLPYLNKDFKSVIIVGGDV
GGVEFGWMLRKLGKEVVLVDKQSSLLPYLDKEVSNAITNYFSKIGVRLYLNRSVKEMKEKEVILENGEKLTADVVYMTFG
RKPSIQGFEEINHNPFVIVDEYLRTSLSNVFAAGDIIGTHTAHEAMYAGKIAATNIMGGKKVFNKQGIPKVVYTHPTIAY
VGSMEGKCVKINLVELGRAITEKEIDGFLKLCIKDDKIVGAQAFMKDAEEVISLISFLIRYNIKVNEIKDYVAPHPSYIE
ALTELLNRL
>Mature_409_residues
MKIAVIGSGPAGVSASLELAKNNKVVLVEKEERLGGTCVLYGCIPSKAMLHPIHLASELEKLGKNITFDLGELRKLGQDA
SYRLSKGVEYMLEDNGVEVIHGIASLRSGQLIVNNETIQVDYVVLATGTYREVVKGIIYSEDLPYLNKDFKSVIIVGGDV
GGVEFGWMLRKLGKEVVLVDKQSSLLPYLDKEVSNAITNYFSKIGVRLYLNRSVKEMKEKEVILENGEKLTADVVYMTFG
RKPSIQGFEEINHNPFVIVDEYLRTSLSNVFAAGDIIGTHTAHEAMYAGKIAATNIMGGKKVFNKQGIPKVVYTHPTIAY
VGSMEGKCVKINLVELGRAITEKEIDGFLKLCIKDDKIVGAQAFMKDAEEVISLISFLIRYNIKVNEIKDYVAPHPSYIE
ALTELLNRL

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=453, Percent_Identity=27.5938189845475, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI50301238, Length=449, Percent_Identity=23.3853006681514, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1786307, Length=448, Percent_Identity=27.9017857142857, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87082354, Length=416, Percent_Identity=26.4423076923077, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789915, Length=426, Percent_Identity=25.3521126760563, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081717, Length=449, Percent_Identity=22.9398663697105, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI32565766, Length=450, Percent_Identity=28, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71983419, Length=424, Percent_Identity=25, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71983429, Length=424, Percent_Identity=25, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=459, Percent_Identity=27.2331154684096, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6325166, Length=446, Percent_Identity=25.3363228699552, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=23.1578947368421, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358499, Length=452, Percent_Identity=29.4247787610619, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=23.2067510548523, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24640553, Length=468, Percent_Identity=25.2136752136752, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24640549, Length=467, Percent_Identity=24.8394004282655, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24640551, Length=467, Percent_Identity=24.8394004282655, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 45197; Mature: 45197

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVIGSGPAGVSASLELAKNNKVVLVEKEERLGGTCVLYGCIPSKAMLHPIHLASELE
CEEEEEECCCCCCCEEEEEECCCCEEEEECHHHCCCEEEEEECCCCCHHHHHHHHHHHHH
KLGKNITFDLGELRKLGQDASYRLSKGVEYMLEDNGVEVIHGIASLRSGQLIVNNETIQV
HHCCCCEECHHHHHHCCCCCCHHHHCCCEEEECCCCHHHHHHHHHCCCCEEEEECCEEEE
DYVVLATGTYREVVKGIIYSEDLPYLNKDFKSVIIVGGDVGGVEFGWMLRKLGKEVVLVD
EEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEE
KQSSLLPYLDKEVSNAITNYFSKIGVRLYLNRSVKEMKEKEVILENGEKLTADVVYMTFG
CCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEEEEEEC
RKPSIQGFEEINHNPFVIVDEYLRTSLSNVFAAGDIIGTHTAHEAMYAGKIAATNIMGGK
CCCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHHCCEEECCHHHHHHHHCCEEEEECCCCH
KVFNKQGIPKVVYTHPTIAYVGSMEGKCVKINLVELGRAITEKEIDGFLKLCIKDDKIVG
HHHCCCCCCEEEEECCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEC
AQAFMKDAEEVISLISFLIRYNIKVNEIKDYVAPHPSYIEALTELLNRL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKIAVIGSGPAGVSASLELAKNNKVVLVEKEERLGGTCVLYGCIPSKAMLHPIHLASELE
CEEEEEECCCCCCCEEEEEECCCCEEEEECHHHCCCEEEEEECCCCCHHHHHHHHHHHHH
KLGKNITFDLGELRKLGQDASYRLSKGVEYMLEDNGVEVIHGIASLRSGQLIVNNETIQV
HHCCCCEECHHHHHHCCCCCCHHHHCCCEEEECCCCHHHHHHHHHCCCCEEEEECCEEEE
DYVVLATGTYREVVKGIIYSEDLPYLNKDFKSVIIVGGDVGGVEFGWMLRKLGKEVVLVD
EEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEE
KQSSLLPYLDKEVSNAITNYFSKIGVRLYLNRSVKEMKEKEVILENGEKLTADVVYMTFG
CCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEEEEEEC
RKPSIQGFEEINHNPFVIVDEYLRTSLSNVFAAGDIIGTHTAHEAMYAGKIAATNIMGGK
CCCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHHCCEEECCHHHHHHHHCCEEEEECCCCH
KVFNKQGIPKVVYTHPTIAYVGSMEGKCVKINLVELGRAITEKEIDGFLKLCIKDDKIVG
HHHCCCCCCEEEEECCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEC
AQAFMKDAEEVISLISFLIRYNIKVNEIKDYVAPHPSYIEALTELLNRL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339281; 1581034 [H]