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The map label for this gene is proP [H]

Identifier: 15899292

GI number: 15899292

Start: 2331433

End: 2332599

Strand: Direct

Name: proP [H]

Synonym: SSO2558

Alternate gene names: 15899292

Gene position: 2331433-2332599 (Clockwise)

Preceding gene: 15899291

Following gene: 15899293

Centisome position: 77.92

GC content: 37.28

Gene sequence:

>1167_bases
ATGGATGGGTACGATTTAGGTGCTGTTGTTATTACAGCAACGATATTAGGTAAATTGTTCTATCCCGGGATAGGTTTGCT
AAGTGCAGTTTTGCCCATAGTGTTTACTGTAATCTCTAGGCCATTAGGTGGATTTGTTTTTGGTTATGTTGGAGATAAAT
ACGGCAGAAGAGTTAGCTTACTAATTTCGGTATTAGGTTACTCTTTATCTATAGGCTTAACTTCTATATTACCTACTTAT
GCCCAAATAGGGATTTTAGCTGCCGTTATAATTTCAATTCTAAGATTTATACAAGGTATATTTATAGGAGGAGACGTTGC
GGGTAGTTTCACTATAGTAATGGAAAGCCTTAACAATTATAGAGGTCTATTTTCCGGTATAATGCAATCTGGAGTTTTGG
TTGGTTTTGTTGGAGTTGACTTCTTATTTACTTATTTAGCGTCTGTTACTGGAGCACAGTTTATATCGACTTATTGGAGG
TTAATTTTCGCCATTGGAGTAATACCAGCAATATTGGCTGCCTTAATTAGATTCAGGATGACAGAACCTAGTGTCTGGCT
TAAGATAAAACATTCTGCAAATCCCTTAAAAGGCCTAAGAGAACTTCCGCAACCGTTTCTAGTTATGGTAGGCTTCTGGT
TGGCAATTTACGCTGGACCTCAATTAGTACCTACATTGTTTGGGCAAGTGATGAAGTTGCCTCCTTCGTATTACGGTCCT
TTAGTTCTCTATATGAATTTAATAGGGATACCATCCATGTTGGTTGCAGGAGTTCTCTCGGATTATATAGGGAGAAGAAT
GATGGGTATAATAGGTTCGATATTTGCTGCAATAGGTTCATTAACTTTTTATTACTTTATTCAAATTCACAACATTAACC
TATTATATCTTATATTACTTTTCGGTTTTCTGGTAAACTTGCCCTCTTCTATAACTCCTGCATTTTTATCAGAGAGATTT
AAGACGTTTACAAGAGCTTTGGGAGTTGGCACCGCGTACAATGGAGCATATATAATAGCGGGTTTCGCTCCGATTTACGT
TTCCATTCTTTCGAACTTTATGAATCCATTTAATGCAGCTACAACAGTAGCAGTTATCGGCTTCATTATAGCCATAACTG
GACTTATTGCGGGACCGGAAACTTATAAGACAAGTCTAGAAGAGTGA

Upstream 100 bases:

>100_bases
CTCGGTGATCCAGGTCCTATCCTTCTGGCAATATTAATATTTATCATTCTGTTTATCTATTCGTTCAGATAATTACATTC
CGGACACTCTCTCGTGGGTA

Downstream 100 bases:

>100_bases
TTTGTTTTTTAATGTTAAGTCAAATATTTTCTTATGAAGATAGCAGTTATAGGTTCAGGTCCAGCTGGAGTATCAGCTTC
ATTAGAATTAGCCAAAAACA

Product: sugar transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MDGYDLGAVVITATILGKLFYPGIGLLSAVLPIVFTVISRPLGGFVFGYVGDKYGRRVSLLISVLGYSLSIGLTSILPTY
AQIGILAAVIISILRFIQGIFIGGDVAGSFTIVMESLNNYRGLFSGIMQSGVLVGFVGVDFLFTYLASVTGAQFISTYWR
LIFAIGVIPAILAALIRFRMTEPSVWLKIKHSANPLKGLRELPQPFLVMVGFWLAIYAGPQLVPTLFGQVMKLPPSYYGP
LVLYMNLIGIPSMLVAGVLSDYIGRRMMGIIGSIFAAIGSLTFYYFIQIHNINLLYLILLFGFLVNLPSSITPAFLSERF
KTFTRALGVGTAYNGAYIIAGFAPIYVSILSNFMNPFNAATTVAVIGFIIAITGLIAGPETYKTSLEE

Sequences:

>Translated_388_residues
MDGYDLGAVVITATILGKLFYPGIGLLSAVLPIVFTVISRPLGGFVFGYVGDKYGRRVSLLISVLGYSLSIGLTSILPTY
AQIGILAAVIISILRFIQGIFIGGDVAGSFTIVMESLNNYRGLFSGIMQSGVLVGFVGVDFLFTYLASVTGAQFISTYWR
LIFAIGVIPAILAALIRFRMTEPSVWLKIKHSANPLKGLRELPQPFLVMVGFWLAIYAGPQLVPTLFGQVMKLPPSYYGP
LVLYMNLIGIPSMLVAGVLSDYIGRRMMGIIGSIFAAIGSLTFYYFIQIHNINLLYLILLFGFLVNLPSSITPAFLSERF
KTFTRALGVGTAYNGAYIIAGFAPIYVSILSNFMNPFNAATTVAVIGFIIAITGLIAGPETYKTSLEE
>Mature_388_residues
MDGYDLGAVVITATILGKLFYPGIGLLSAVLPIVFTVISRPLGGFVFGYVGDKYGRRVSLLISVLGYSLSIGLTSILPTY
AQIGILAAVIISILRFIQGIFIGGDVAGSFTIVMESLNNYRGLFSGIMQSGVLVGFVGVDFLFTYLASVTGAQFISTYWR
LIFAIGVIPAILAALIRFRMTEPSVWLKIKHSANPLKGLRELPQPFLVMVGFWLAIYAGPQLVPTLFGQVMKLPPSYYGP
LVLYMNLIGIPSMLVAGVLSDYIGRRMMGIIGSIFAAIGSLTFYYFIQIHNINLLYLILLFGFLVNLPSSITPAFLSERF
KTFTRALGVGTAYNGAYIIAGFAPIYVSILSNFMNPFNAATTVAVIGFIIAITGLIAGPETYKTSLEE

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=405, Percent_Identity=26.6666666666667, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788292, Length=407, Percent_Identity=27.027027027027, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788942, Length=354, Percent_Identity=28.8135593220339, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082404, Length=348, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082231, Length=204, Percent_Identity=28.4313725490196, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789941, Length=187, Percent_Identity=29.9465240641711, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 41972; Mature: 41972

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGYDLGAVVITATILGKLFYPGIGLLSAVLPIVFTVISRPLGGFVFGYVGDKYGRRVSL
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHH
LISVLGYSLSIGLTSILPTYAQIGILAAVIISILRFIQGIFIGGDVAGSFTIVMESLNNY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RGLFSGIMQSGVLVGFVGVDFLFTYLASVTGAQFISTYWRLIFAIGVIPAILAALIRFRM
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TEPSVWLKIKHSANPLKGLRELPQPFLVMVGFWLAIYAGPQLVPTLFGQVMKLPPSYYGP
CCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHH
LVLYMNLIGIPSMLVAGVLSDYIGRRMMGIIGSIFAAIGSLTFYYFIQIHNINLLYLILL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHH
FGFLVNLPSSITPAFLSERFKTFTRALGVGTAYNGAYIIAGFAPIYVSILSNFMNPFNAA
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCHHHHH
TTVAVIGFIIAITGLIAGPETYKTSLEE
HHHHHHHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]