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Identifier: 15899258

GI number: 15899258

Start: 2291000

End: 2291944

Strand: Reverse

Name: php

Synonym: SSO2522

Alternate gene names: 15899258

Gene position: 2291944-2291000 (Counterclockwise)

Preceding gene: 15899260

Following gene: 15899257

Centisome position: 76.6

GC content: 36.3

Gene sequence:

>945_bases
ATGAGAATACCATTAGTTGGGAAAGATTCAATAGAATCTAAGGACATAGGATTTACGCTAATTCATGAACATTTAAGAGT
TTTTAGCGAAGCGGTCAGACAACAATGGCCCCATCTATATAACGAAGATGAGGAGTTCAGAAACGCTGTAAATGAGGTTA
AAAGGGCAATGCAATTTGGAGTAAAGACTATAGTAGATCCCACTGTAATGGGATTGGGTAGGGACATCAGATTTATGGAA
AAAGTGGTTAAGGCTACCGGGATAAATTTAGTTGCGGGGACGGGGATTTACATATATATCGACTTACCTTTCTATTTCTT
AAATAGGTCAATTGATGAGATAGCTGACTTGTTTATTCATGATATAAAAGAGGGAATACAAGGTACTCTCAATAAAGCTG
GCTTCGTAAAGATAGCTGCAGATGAACCTGGGATCACAAAGGATGTGGAGAAGGTAATAAGGGCTGCTGCCATAGCAAAC
AAAGAGACTAAAGTACCAATAATTACCCACTCTAACGCTCACAATAACACCGGATTAGAACAGCAAAGAATATTGACTGA
AGAAGGTGTTGATCCAGGGAAAATATTAATAGGTCATTTAGGTGATACAGATAATATAGATTACATAAAGAAGATAGCAG
ATAAGGGATCCTTTATTGGATTAGATAGATATGGTTTAGATTTATTCCTACCTGTTGATAAGAGAAATGAAACGACCTTA
AGACTAATCAAAGATGGTTATTCAGATAAGATAATGATCTCTCACGATTATTGTTGCACAATCGACTGGGGAACTGCAAA
ACCAGAATATAAACCTAAGCTTGCTCCAAGATGGAGTATAACTCTAATATTTGAGGATACGATACCGTTCTTAAAGAGAA
ATGGAGTGAATGAAGAGGTTATAGCTACAATATTTAAGGAAAATCCGAAAAAGTTCTTCAGCTAA

Upstream 100 bases:

>100_bases
CATATAATATACTTTGATTCATAGGAAAATTTAGATATATGCACTAATAAACCTTTATTCACAGAAAAGCTTATCTACTA
TGGATAAAATAGACTAGTAT

Downstream 100 bases:

>100_bases
ATATTTATTTTTAACTTAACACTTTTATTATAATATGCCACTAGATCCACAAGTTAAGGAGCTCTTAACTAAAATAAATT
TGCTATTACCTACAACTCCT

Product: aryldialkylphosphatase (phosphotriesterase) (paraoxonase), putative (PHP)

Products: NA

Alternate protein names: Paraoxonase; SsoPox; Phosphotriesterase-like lactonase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRIPLVGKDSIESKDIGFTLIHEHLRVFSEAVRQQWPHLYNEDEEFRNAVNEVKRAMQFGVKTIVDPTVMGLGRDIRFME
KVVKATGINLVAGTGIYIYIDLPFYFLNRSIDEIADLFIHDIKEGIQGTLNKAGFVKIAADEPGITKDVEKVIRAAAIAN
KETKVPIITHSNAHNNTGLEQQRILTEEGVDPGKILIGHLGDTDNIDYIKKIADKGSFIGLDRYGLDLFLPVDKRNETTL
RLIKDGYSDKIMISHDYCCTIDWGTAKPEYKPKLAPRWSITLIFEDTIPFLKRNGVNEEVIATIFKENPKKFFS

Sequences:

>Translated_314_residues
MRIPLVGKDSIESKDIGFTLIHEHLRVFSEAVRQQWPHLYNEDEEFRNAVNEVKRAMQFGVKTIVDPTVMGLGRDIRFME
KVVKATGINLVAGTGIYIYIDLPFYFLNRSIDEIADLFIHDIKEGIQGTLNKAGFVKIAADEPGITKDVEKVIRAAAIAN
KETKVPIITHSNAHNNTGLEQQRILTEEGVDPGKILIGHLGDTDNIDYIKKIADKGSFIGLDRYGLDLFLPVDKRNETTL
RLIKDGYSDKIMISHDYCCTIDWGTAKPEYKPKLAPRWSITLIFEDTIPFLKRNGVNEEVIATIFKENPKKFFS
>Mature_314_residues
MRIPLVGKDSIESKDIGFTLIHEHLRVFSEAVRQQWPHLYNEDEEFRNAVNEVKRAMQFGVKTIVDPTVMGLGRDIRFME
KVVKATGINLVAGTGIYIYIDLPFYFLNRSIDEIADLFIHDIKEGIQGTLNKAGFVKIAADEPGITKDVEKVIRAAAIAN
KETKVPIITHSNAHNNTGLEQQRILTEEGVDPGKILIGHLGDTDNIDYIKKIADKGSFIGLDRYGLDLFLPVDKRNETTL
RLIKDGYSDKIMISHDYCCTIDWGTAKPEYKPKLAPRWSITLIFEDTIPFLKRNGVNEEVIATIFKENPKKFFS

Specific function: Has a low paraoxonase activity. Also active, but with a lower activity, against other oregano-phosphorus insecticides such as Dursban, Coumaphos, pNP-butanoate or parathion

COG id: COG1735

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphotriesterase family

Homologues:

Organism=Homo sapiens, GI20070186, Length=344, Percent_Identity=23.546511627907, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI47933341, Length=344, Percent_Identity=23.546511627907, Blast_Score=98, Evalue=9e-21,
Organism=Escherichia coli, GI1789780, Length=302, Percent_Identity=31.4569536423841, Blast_Score=142, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645325, Length=232, Percent_Identity=28.448275862069, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHP_SULSO (Q97VT7)

Other databases:

- EMBL:   AE006641
- EMBL:   AY775568
- PIR:   F90424
- RefSeq:   NP_343863.1
- PDB:   2VC5
- PDB:   2VC7
- PDBsum:   2VC5
- PDBsum:   2VC7
- ProteinModelPortal:   Q97VT7
- GeneID:   1453980
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO2522
- NMPDR:   fig|273057.1.peg.2286
- HOGENOM:   HBG732614
- OMA:   EPIMMKN
- PhylomeDB:   Q97VT7
- ProtClustDB:   CLSK2484533
- BioCyc:   SSOL273057:SSO2522-MONOMER
- InterPro:   IPR017947
- InterPro:   IPR001559
- PANTHER:   PTHR10819

Pfam domain/function: PF02126 PTE

EC number: =3.1.8.1

Molecular weight: Translated: 35565; Mature: 35565

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01322 PHOSPHOTRIESTERASE_1; PS51347 PHOSPHOTRIESTERASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPLVGKDSIESKDIGFTLIHEHLRVFSEAVRQQWPHLYNEDEEFRNAVNEVKRAMQFG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
VKTIVDPTVMGLGRDIRFMEKVVKATGINLVAGTGIYIYIDLPFYFLNRSIDEIADLFIH
HHHHCCHHHHHCCCCHHHHHHHHHHCCCEEEECCCEEEEEECHHHHHCCCHHHHHHHHHH
DIKEGIQGTLNKAGFVKIAADEPGITKDVEKVIRAAAIANKETKVPIITHSNAHNNTGLE
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCH
QQRILTEEGVDPGKILIGHLGDTDNIDYIKKIADKGSFIGLDRYGLDLFLPVDKRNETTL
HHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCHHH
RLIKDGYSDKIMISHDYCCTIDWGTAKPEYKPKLAPRWSITLIFEDTIPFLKRNGVNEEV
HHHHCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHCCCCHHH
IATIFKENPKKFFS
HHHHHHCCCHHHCC
>Mature Secondary Structure
MRIPLVGKDSIESKDIGFTLIHEHLRVFSEAVRQQWPHLYNEDEEFRNAVNEVKRAMQFG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
VKTIVDPTVMGLGRDIRFMEKVVKATGINLVAGTGIYIYIDLPFYFLNRSIDEIADLFIH
HHHHCCHHHHHCCCCHHHHHHHHHHCCCEEEECCCEEEEEECHHHHHCCCHHHHHHHHHH
DIKEGIQGTLNKAGFVKIAADEPGITKDVEKVIRAAAIANKETKVPIITHSNAHNNTGLE
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCH
QQRILTEEGVDPGKILIGHLGDTDNIDYIKKIADKGSFIGLDRYGLDLFLPVDKRNETTL
HHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCHHH
RLIKDGYSDKIMISHDYCCTIDWGTAKPEYKPKLAPRWSITLIFEDTIPFLKRNGVNEEV
HHHHCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHCCCCHHH
IATIFKENPKKFFS
HHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726