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The map label for this gene is lipP-2 [H]

Identifier: 15899257

GI number: 15899257

Start: 2290030

End: 2290965

Strand: Reverse

Name: lipP-2 [H]

Synonym: SSO2521

Alternate gene names: 15899257

Gene position: 2290965-2290030 (Counterclockwise)

Preceding gene: 15899258

Following gene: 15899255

Centisome position: 76.56

GC content: 36.75

Gene sequence:

>936_bases
ATGCCACTAGATCCACAAGTTAAGGAGCTCTTAACTAAAATAAATTTGCTATTACCTACAACTCCTTTAACACCTCAAGA
GTTCAGAAAGGTTAGAAATGAGCTCTTTATAAAGATGTTTAATAACGAAAAGGTTGGGCTATATAATATTCGTGATATGA
CTATCCCAGTTAGGAATGGTGATATAAAAGTTAGGGTCTATTTTCCAAACCAAAGGGAAAACCTTCCCGCTGTAGTGTAT
TATCATGGAGGAGGTTTCGTTTACGGAAATTTAGATACTCATGATAGCGTGTGTAGATTAATATCAAAGTTGTCAAATAC
AATAATAGTTTCAGTGGATTATAGGTTAGCTCCAGAACACAAATTCCCCACACAAGTTTATGACGCATACGATGTGGTTA
AGTGGTTAGCTAATAATGGAGGAAAGCTATCCATAGATACGTCAAAAATTGCAGTTGCTGGAGATAGTGCGGGGGGAAAT
TTATCCACGGTAGTTAGTATCCTTGATAGAGATAATGGAGAAAACGTTGTGAAATACCAAATAATGATATACCCAGTAGT
TAACATGTTAGACTCCTCTCCCTCAATGTATAATTACGGGGATGGCTACTTCCTAACCTATGAGAGAATTTTATGGTACA
ACAAGCAGTATGTTAAAGAAGACAGTGATTATTACAACCCCTTAGCCTCTCCAATATTGGCTGAATCACATAACCTTCCT
CCCGCGTTAATAATTACCGCCGAATATGATCCCCTAAGAGATCAAGGTGAAATGTACGCTCATAAACTGAAGGTAAGTGG
AGTGAAGACAATAAGCTTGAGATATAACGGCATGATCCACGGCTTCGTGAGTTTTTATGAGTATTTAGATACGGGAAAAG
AAGCAATACATCATATTGCATCATCAATTAGAAAAATGTTTAACGTTTTCTATTGA

Upstream 100 bases:

>100_bases
AATGGAGTGAATGAAGAGGTTATAGCTACAATATTTAAGGAAAATCCGAAAAAGTTCTTCAGCTAAATATTTATTTTTAA
CTTAACACTTTTATTATAAT

Downstream 100 bases:

>100_bases
TGATTTTTTACTATAATATTTTTCAGAATCCCTATCTTCTCCACATATAATTCTATACTACTATTTGGCTTTAACGATTT
GCCTATATCTAACCCAGTAC

Product: lipase (lipP-2)

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MPLDPQVKELLTKINLLLPTTPLTPQEFRKVRNELFIKMFNNEKVGLYNIRDMTIPVRNGDIKVRVYFPNQRENLPAVVY
YHGGGFVYGNLDTHDSVCRLISKLSNTIIVSVDYRLAPEHKFPTQVYDAYDVVKWLANNGGKLSIDTSKIAVAGDSAGGN
LSTVVSILDRDNGENVVKYQIMIYPVVNMLDSSPSMYNYGDGYFLTYERILWYNKQYVKEDSDYYNPLASPILAESHNLP
PALIITAEYDPLRDQGEMYAHKLKVSGVKTISLRYNGMIHGFVSFYEYLDTGKEAIHHIASSIRKMFNVFY

Sequences:

>Translated_311_residues
MPLDPQVKELLTKINLLLPTTPLTPQEFRKVRNELFIKMFNNEKVGLYNIRDMTIPVRNGDIKVRVYFPNQRENLPAVVY
YHGGGFVYGNLDTHDSVCRLISKLSNTIIVSVDYRLAPEHKFPTQVYDAYDVVKWLANNGGKLSIDTSKIAVAGDSAGGN
LSTVVSILDRDNGENVVKYQIMIYPVVNMLDSSPSMYNYGDGYFLTYERILWYNKQYVKEDSDYYNPLASPILAESHNLP
PALIITAEYDPLRDQGEMYAHKLKVSGVKTISLRYNGMIHGFVSFYEYLDTGKEAIHHIASSIRKMFNVFY
>Mature_310_residues
PLDPQVKELLTKINLLLPTTPLTPQEFRKVRNELFIKMFNNEKVGLYNIRDMTIPVRNGDIKVRVYFPNQRENLPAVVYY
HGGGFVYGNLDTHDSVCRLISKLSNTIIVSVDYRLAPEHKFPTQVYDAYDVVKWLANNGGKLSIDTSKIAVAGDSAGGNL
STVVSILDRDNGENVVKYQIMIYPVVNMLDSSPSMYNYGDGYFLTYERILWYNKQYVKEDSDYYNPLASPILAESHNLPP
ALIITAEYDPLRDQGEMYAHKLKVSGVKTISLRYNGMIHGFVSFYEYLDTGKEAIHHIASSIRKMFNVFY

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=289, Percent_Identity=30.1038062283737, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI68299767, Length=292, Percent_Identity=28.4246575342466, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI68051721, Length=297, Percent_Identity=26.9360269360269, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI157041239, Length=172, Percent_Identity=31.9767441860465, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI226423947, Length=305, Percent_Identity=26.2295081967213, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI61966717, Length=137, Percent_Identity=27.7372262773723, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786682, Length=306, Percent_Identity=27.1241830065359, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI72001146, Length=145, Percent_Identity=38.6206896551724, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71996133, Length=197, Percent_Identity=33.502538071066, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17540028, Length=185, Percent_Identity=31.8918918918919, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17567059, Length=149, Percent_Identity=32.2147651006711, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI115533412, Length=90, Percent_Identity=38.8888888888889, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115533410, Length=90, Percent_Identity=38.8888888888889, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656084, Length=102, Percent_Identity=37.2549019607843, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24656076, Length=102, Percent_Identity=37.2549019607843, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20130169, Length=102, Percent_Identity=37.2549019607843, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 35509; Mature: 35378

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDPQVKELLTKINLLLPTTPLTPQEFRKVRNELFIKMFNNEKVGLYNIRDMTIPVRNG
CCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHEEEEECCCCEEEEEEEEEEEEEECC
DIKVRVYFPNQRENLPAVVYYHGGGFVYGNLDTHDSVCRLISKLSNTIIVSVDYRLAPEH
CEEEEEECCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECCCC
KFPTQVYDAYDVVKWLANNGGKLSIDTSKIAVAGDSAGGNLSTVVSILDRDNGENVVKYQ
CCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEE
IMIYPVVNMLDSSPSMYNYGDGYFLTYERILWYNKQYVKEDSDYYNPLASPILAESHNLP
EEEEEHHHHHCCCCCCCCCCCCEEEEEEEHHHHCHHHHCCCCCHHCHHHCHHHHCCCCCC
PALIITAEYDPLRDQGEMYAHKLKVSGVKTISLRYNGMIHGFVSFYEYLDTGKEAIHHIA
CEEEEEECCCCCCCCCHHHHHEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
SSIRKMFNVFY
HHHHHHHHHCC
>Mature Secondary Structure 
PLDPQVKELLTKINLLLPTTPLTPQEFRKVRNELFIKMFNNEKVGLYNIRDMTIPVRNG
CCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHEEEEECCCCEEEEEEEEEEEEEECC
DIKVRVYFPNQRENLPAVVYYHGGGFVYGNLDTHDSVCRLISKLSNTIIVSVDYRLAPEH
CEEEEEECCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECCCC
KFPTQVYDAYDVVKWLANNGGKLSIDTSKIAVAGDSAGGNLSTVVSILDRDNGENVVKYQ
CCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEE
IMIYPVVNMLDSSPSMYNYGDGYFLTYERILWYNKQYVKEDSDYYNPLASPILAESHNLP
EEEEEHHHHHCCCCCCCCCCCCEEEEEEEHHHHCHHHHCCCCCHHCHHHCHHHHCCCCCC
PALIITAEYDPLRDQGEMYAHKLKVSGVKTISLRYNGMIHGFVSFYEYLDTGKEAIHHIA
CEEEEEECCCCCCCCCHHHHHEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
SSIRKMFNVFY
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA