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The map label for this gene is est [H]

Identifier: 15899253

GI number: 15899253

Start: 2286281

End: 2287036

Strand: Direct

Name: est [H]

Synonym: SSO2517

Alternate gene names: 15899253

Gene position: 2286281-2287036 (Clockwise)

Preceding gene: 15899252

Following gene: 15899254

Centisome position: 76.41

GC content: 39.29

Gene sequence:

>756_bases
TTGGAAACTGAAATACCTATAAGAATATATTATCCAGATAATAAACGAGATCATCCTATAATTCTTCATTTTCATGGTGG
CGCATGGATTTTAGGCAGTATCGAGACTGAGGATAGTGTTTCTAGAATATTGGCTAATTCTTGTAACTGTATAGTGATTT
CAGTTAATTATAGACTAGCACCAGAACATAAGTTCCCTGCGGCCGTAACGGACTGCTTTGACTCTATTAAATGGACATAT
GAGAACGCTGAAAGTATAGGTGGTCATCCGAATCGAATCGCAGTTTTCGGAATTAGCGCTGGAGGTAACTTAGCTGCAGC
TACGTCTATTCTTTCCAGAGATCAAGGGATAAAATTAAGGGCTCAAGCACTTGTGGTTCCCTTCGTATATCTAGACTTAG
CCTCTACATCAATGACTGAATATAGAAAAGGCTATTTCTTGGACATTAACGTACCCATAGATTACGGAATAATGATGTAC
ATAAGAGACGAGAAGGATCTTCTTAATCCCATGTTCGTTCCTTTAATTGCAGAAGATCTCTCCAACTTACCACAAGCCAT
TATTGTCACAGCCGAATATGATCCCTTAAGAGACCAAGGGGAGGCTTATGCAAAGAGACTAATGGAGGCTGGGGTTTTAA
CCTTAAGTTTTAGAGTTAACGGTATGGTTCACGGCTTTTTAGGTTCTCCTAATATTAGTAGGCTAGTTAGTGTAATGGTA
GGTTCTGCGCTAAAGGACATCTTAATGAGGGGTTAA

Upstream 100 bases:

>100_bases
ATATTCATTACAAGAAGTTAGAGAAAAACTAAACAAACTCTTAGCAGAAGCTACACCAAAGGACTCTGTATATAAAATTG
AGGATAGGAAGATTAAAGGG

Downstream 100 bases:

>100_bases
CACTCATGTATGAAGAATGGAAAATCGTCAAAAGGGAAGCACCAATCTTGGGTAATGATCAGCTTATAGAGAACATATGG
AAAATGAAGAGAGAAGATTC

Product: carboxylesterase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
METEIPIRIYYPDNKRDHPIILHFHGGAWILGSIETEDSVSRILANSCNCIVISVNYRLAPEHKFPAAVTDCFDSIKWTY
ENAESIGGHPNRIAVFGISAGGNLAAATSILSRDQGIKLRAQALVVPFVYLDLASTSMTEYRKGYFLDINVPIDYGIMMY
IRDEKDLLNPMFVPLIAEDLSNLPQAIIVTAEYDPLRDQGEAYAKRLMEAGVLTLSFRVNGMVHGFLGSPNISRLVSVMV
GSALKDILMRG

Sequences:

>Translated_251_residues
METEIPIRIYYPDNKRDHPIILHFHGGAWILGSIETEDSVSRILANSCNCIVISVNYRLAPEHKFPAAVTDCFDSIKWTY
ENAESIGGHPNRIAVFGISAGGNLAAATSILSRDQGIKLRAQALVVPFVYLDLASTSMTEYRKGYFLDINVPIDYGIMMY
IRDEKDLLNPMFVPLIAEDLSNLPQAIIVTAEYDPLRDQGEAYAKRLMEAGVLTLSFRVNGMVHGFLGSPNISRLVSVMV
GSALKDILMRG
>Mature_251_residues
METEIPIRIYYPDNKRDHPIILHFHGGAWILGSIETEDSVSRILANSCNCIVISVNYRLAPEHKFPAAVTDCFDSIKWTY
ENAESIGGHPNRIAVFGISAGGNLAAATSILSRDQGIKLRAQALVVPFVYLDLASTSMTEYRKGYFLDINVPIDYGIMMY
IRDEKDLLNPMFVPLIAEDLSNLPQAIIVTAEYDPLRDQGEAYAKRLMEAGVLTLSFRVNGMVHGFLGSPNISRLVSVMV
GSALKDILMRG

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=301, Percent_Identity=28.2392026578073, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI206597554, Length=298, Percent_Identity=28.1879194630872, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI157041239, Length=307, Percent_Identity=27.3615635179153, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI68051721, Length=293, Percent_Identity=28.6689419795222, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI61966717, Length=129, Percent_Identity=31.0077519379845, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI226423947, Length=301, Percent_Identity=27.5747508305648, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI21328446, Length=104, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1786682, Length=248, Percent_Identity=29.8387096774194, Blast_Score=91, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI72001146, Length=148, Percent_Identity=38.5135135135135, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17540028, Length=250, Percent_Identity=28, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71996133, Length=246, Percent_Identity=28.0487804878049, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17567059, Length=129, Percent_Identity=34.1085271317829, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533410, Length=127, Percent_Identity=32.2834645669291, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533412, Length=127, Percent_Identity=32.2834645669291, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24656084, Length=100, Percent_Identity=32, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24656076, Length=100, Percent_Identity=32, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI20130169, Length=100, Percent_Identity=32, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 27811; Mature: 27811

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METEIPIRIYYPDNKRDHPIILHFHGGAWILGSIETEDSVSRILANSCNCIVISVNYRLA
CCCCCEEEEECCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHCCCCEEEEEEEEEEC
PEHKFPAAVTDCFDSIKWTYENAESIGGHPNRIAVFGISAGGNLAAATSILSRDQGIKLR
CCCCCCHHHHHHHHHHEEEECCHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEE
AQALVVPFVYLDLASTSMTEYRKGYFLDINVPIDYGIMMYIRDEKDLLNPMFVPLIAEDL
EHEEEEEHHEEEHHHCHHHHHHCCEEEEEECCCCCEEEEEEECCHHHCCHHHHHHHHHHH
SNLPQAIIVTAEYDPLRDQGEAYAKRLMEAGVLTLSFRVNGMVHGFLGSPNISRLVSVMV
HCCCEEEEEEECCCCCCCHHHHHHHHHHHCCEEEEEEEECCEEEEECCCCCHHHHHHHHH
GSALKDILMRG
HHHHHHHHHCC
>Mature Secondary Structure
METEIPIRIYYPDNKRDHPIILHFHGGAWILGSIETEDSVSRILANSCNCIVISVNYRLA
CCCCCEEEEECCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHCCCCEEEEEEEEEEC
PEHKFPAAVTDCFDSIKWTYENAESIGGHPNRIAVFGISAGGNLAAATSILSRDQGIKLR
CCCCCCHHHHHHHHHHEEEECCHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEE
AQALVVPFVYLDLASTSMTEYRKGYFLDINVPIDYGIMMYIRDEKDLLNPMFVPLIAEDL
EHEEEEEHHEEEHHHCHHHHHHCCEEEEEECCCCCEEEEEEECCHHHCCHHHHHHHHHHH
SNLPQAIIVTAEYDPLRDQGEAYAKRLMEAGVLTLSFRVNGMVHGFLGSPNISRLVSVMV
HCCCEEEEEEECCCCCCCHHHHHHHHHHHCCEEEEEEEECCEEEEECCCCCHHHHHHHHH
GSALKDILMRG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]