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The map label for this gene is ogt [H]

Identifier: 15899228

GI number: 15899228

Start: 2258839

End: 2259294

Strand: Direct

Name: ogt [H]

Synonym: SSO2487

Alternate gene names: 15899228

Gene position: 2258839-2259294 (Clockwise)

Preceding gene: 15899227

Following gene: 15899229

Centisome position: 75.49

GC content: 37.72

Gene sequence:

>456_bases
GTGCTAGTGTATGGGTTGTATAAAAGTCCTCTTGGCTATATAACTGTTGCTAAGGACGATAAGGGATTTATTATGTTGGA
TTTCTGTGACTGTGTGGAAGGAAATTCAAGAGACGATAGTAGTTTTACTGAATTCTTCCATAAGCTAGATCTCTACTTTG
AGGGTAAGCCAATAAACTTAAGGGAGCCTATTAACCTAAAGACCTATCCTTTTAGATTATCAGTATTTAAAGAAGTAATG
AAGATCCCCTGGGGAAAAGTTATGACGTATAAACAGATAGCAGATTCACTGGGAACTTCTCCAAGAGCTGTTGGTATGGC
TTTATCTAAAAATCCCATTCTTTTAATCATACCATGCCACCGAGTAATAGCTGAAAATGGTATTGGAGGATACTCCAGAG
GAGTAAAGTTGAAGAGAGCCTTATTGGAACTAGAGGGAGTCAAAATACCAGAATGA

Upstream 100 bases:

>100_bases
TTATTGATTACCGAGATTGCGCAAAGGGATGTAGAACAAACTACAGTAAAAGCGTTAAAATAGTGAATTTTTTATAACAA
TATATACTATATTAATACTT

Downstream 100 bases:

>100_bases
TGTGGAAAAATTTAACAGTATTAACCAATTAATTTTTAACAAAAAGAGCTTTTATATCGTATTCGAGTTACTTGAGCATT
CTCCAAAGTCCGAATAGATA

Product: methylated DNA protein cysteine S-methyltransferase. (DNA 6 O methylguanine)(protein L cysteine S methyltransferase) (ogt)

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MLVYGLYKSPLGYITVAKDDKGFIMLDFCDCVEGNSRDDSSFTEFFHKLDLYFEGKPINLREPINLKTYPFRLSVFKEVM
KIPWGKVMTYKQIADSLGTSPRAVGMALSKNPILLIIPCHRVIAENGIGGYSRGVKLKRALLELEGVKIPE

Sequences:

>Translated_151_residues
MLVYGLYKSPLGYITVAKDDKGFIMLDFCDCVEGNSRDDSSFTEFFHKLDLYFEGKPINLREPINLKTYPFRLSVFKEVM
KIPWGKVMTYKQIADSLGTSPRAVGMALSKNPILLIIPCHRVIAENGIGGYSRGVKLKRALLELEGVKIPE
>Mature_151_residues
MLVYGLYKSPLGYITVAKDDKGFIMLDFCDCVEGNSRDDSSFTEFFHKLDLYFEGKPINLREPINLKTYPFRLSVFKEVM
KIPWGKVMTYKQIADSLGTSPRAVGMALSKNPILLIIPCHRVIAENGIGGYSRGVKLKRALLELEGVKIPE

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=44.6601941747573, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1788542, Length=166, Percent_Identity=27.710843373494, Blast_Score=69, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=39.0243902439024, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=39.0243902439024, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17037; Mature: 17037

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVYGLYKSPLGYITVAKDDKGFIMLDFCDCVEGNSRDDSSFTEFFHKLDLYFEGKPINL
CEEEEEECCCCCEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHEEECCCCCCC
REPINLKTYPFRLSVFKEVMKIPWGKVMTYKQIADSLGTSPRAVGMALSKNPILLIIPCH
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCH
RVIAENGIGGYSRGVKLKRALLELEGVKIPE
HHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLVYGLYKSPLGYITVAKDDKGFIMLDFCDCVEGNSRDDSSFTEFFHKLDLYFEGKPINL
CEEEEEECCCCCEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHEEECCCCCCC
REPINLKTYPFRLSVFKEVMKIPWGKVMTYKQIADSLGTSPRAVGMALSKNPILLIIPCH
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCH
RVIAENGIGGYSRGVKLKRALLELEGVKIPE
HHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA