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The map label for this gene is thrS [H]

Identifier: 15899227

GI number: 15899227

Start: 2257164

End: 2258801

Strand: Direct

Name: thrS [H]

Synonym: SSO2486

Alternate gene names: 15899227

Gene position: 2257164-2258801 (Clockwise)

Preceding gene: 15899222

Following gene: 15899228

Centisome position: 75.43

GC content: 37.55

Gene sequence:

>1638_bases
ATGGAAAGTTATAAACCAGTGTGGCTAAAGGGTGCGGTAATTTTAGCCATAAACCTAATAGATAAGGGATATAAGCCAGT
TGCTGTTGGCCTAGGTGAAAGGGATTTCTACATTGATGTGAAATCAGACACTAGCATTACCTTAGATGAGGTAAAGAAGG
CAATCAATGAGAACGTTCTAGCTAATGTATCCATTGAGAATAATCAGATAGTGTATAAAGGAAACAAGGTTTCAATAATA
GAGGATAAGGTTTCAATTTCGACCAATTTAAATCCTAAGTATTTTGAAATTTTAAATATCTCAACTCATCATCCAAATCC
CAATGAACAATACGTCAGAATTAGGGGTGTGGCTTTCGAGACTGAAGAACAGTTAAAGGATTACCTTTCTTGGCTAGAAA
AGGCTGAAGAGACTGACCATAGGCTGATTGGAGAAAAGTTAGACTTATTTAGCTTTCATGAGGAAGCTGGATCAGGATTA
GTACTATTTCATCCTAAGGGACAAACGATAAGAAATGAACTCATTGCGTTTATGAGAGAGATAAATGATAGCATGGGGTA
TCAAGAAGTTTATACTAGCCACGTTTTCAAGACTGATATATGGAAAATATCCGGTCATTATACCTTGTATAGGGATAAAC
TAATTGTCTTTAACATGGAAGGTGATGAGTATGGAGTAAAACCTATGAATTGTCCTGCTCACATTCTAATTTACAAATCT
AAACCTAGAACCTATCGTGATCTTCCAATCAGATTTTCAGAGTTTGGCCATGTATATAGGTGGGAAAAGAAAGGAGAATT
ATATGGGTTACTGAGAGTTAGAGGTTTCGTCCAAGACGATGGTCACATTTTCCTTAGGGAAGATCAACTAAGGGAAGAAA
TTAAGATGCTTATTTCTAAGACAGTGGAAGTTTGGCACAAGTTCGGCTTCAAAGATGACGATATTAAGCCTTATTTGAGT
ACAAGGCCGGATGAGAGTATAGGTTCTGATGAACTGTGGGAAAAAGCTACTAACGCGCTGATTTCCGCGTTGCAAGAATC
GGGCTTAAAATTCGGTATTAAGGAAAAAGAAGGTGCGTTTTATGGGCCTAAAATCGACTTCGAGATAAGGGATAGTCTAG
GTAGATGGTGGCAATTATCCACAATACAAGTTGACTTCAACTTACCTGAGAGGTTTAAGTTGGAGTACATTGATAAGGAT
GGGATAAAGAAGAGGCCAGTAATGGTACATAGAGCAATATATGGATCAATAGATAGATTTGTTGCAATACTACTTGAGCA
CTTTAAGGGTAAGCTACCAACGTGGTTAAGTTCAGTCCAGGTTAGGGTTTTACCGATAACTGATGAAGTAAATGAATACG
CTGAAAAAGTGCTAAACGATATGAGAAAGAGGAGAATAAGAGCAGAGATTGATTATGCAGGTGAGACCTTAAGTAAGAGG
ATTAAGAACGCATACGATCAAGGAGTTCCATACATATTGATAGTAGGTAAGAAAGAAGCTAGTGAGGGTACAGTAACTGT
TAGAGCTAGGGGTAACATTGAAGTGAGGAACGTTAAGTTTGAAAAGTTCCTAGAGTTATTGATTACCGAGATTGCGCAAA
GGGATGTAGAACAAACTACAGTAAAAGCGTTAAAATAG

Upstream 100 bases:

>100_bases
CGAAGCCTGTATGACCACCACCACTGGCTATTATTAAGATTCTTGTCACAATACTCTTTGTAACAGCTAAAGATTTAAGT
TAAATGACATAGCGATATTT

Downstream 100 bases:

>100_bases
TGAATTTTTTATAACAATATATACTATATTAATACTTGTGCTAGTGTATGGGTTGTATAAAAGTCCTCTTGGCTATATAA
CTGTTGCTAAGGACGATAAG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MESYKPVWLKGAVILAINLIDKGYKPVAVGLGERDFYIDVKSDTSITLDEVKKAINENVLANVSIENNQIVYKGNKVSII
EDKVSISTNLNPKYFEILNISTHHPNPNEQYVRIRGVAFETEEQLKDYLSWLEKAEETDHRLIGEKLDLFSFHEEAGSGL
VLFHPKGQTIRNELIAFMREINDSMGYQEVYTSHVFKTDIWKISGHYTLYRDKLIVFNMEGDEYGVKPMNCPAHILIYKS
KPRTYRDLPIRFSEFGHVYRWEKKGELYGLLRVRGFVQDDGHIFLREDQLREEIKMLISKTVEVWHKFGFKDDDIKPYLS
TRPDESIGSDELWEKATNALISALQESGLKFGIKEKEGAFYGPKIDFEIRDSLGRWWQLSTIQVDFNLPERFKLEYIDKD
GIKKRPVMVHRAIYGSIDRFVAILLEHFKGKLPTWLSSVQVRVLPITDEVNEYAEKVLNDMRKRRIRAEIDYAGETLSKR
IKNAYDQGVPYILIVGKKEASEGTVTVRARGNIEVRNVKFEKFLELLITEIAQRDVEQTTVKALK

Sequences:

>Translated_545_residues
MESYKPVWLKGAVILAINLIDKGYKPVAVGLGERDFYIDVKSDTSITLDEVKKAINENVLANVSIENNQIVYKGNKVSII
EDKVSISTNLNPKYFEILNISTHHPNPNEQYVRIRGVAFETEEQLKDYLSWLEKAEETDHRLIGEKLDLFSFHEEAGSGL
VLFHPKGQTIRNELIAFMREINDSMGYQEVYTSHVFKTDIWKISGHYTLYRDKLIVFNMEGDEYGVKPMNCPAHILIYKS
KPRTYRDLPIRFSEFGHVYRWEKKGELYGLLRVRGFVQDDGHIFLREDQLREEIKMLISKTVEVWHKFGFKDDDIKPYLS
TRPDESIGSDELWEKATNALISALQESGLKFGIKEKEGAFYGPKIDFEIRDSLGRWWQLSTIQVDFNLPERFKLEYIDKD
GIKKRPVMVHRAIYGSIDRFVAILLEHFKGKLPTWLSSVQVRVLPITDEVNEYAEKVLNDMRKRRIRAEIDYAGETLSKR
IKNAYDQGVPYILIVGKKEASEGTVTVRARGNIEVRNVKFEKFLELLITEIAQRDVEQTTVKALK
>Mature_545_residues
MESYKPVWLKGAVILAINLIDKGYKPVAVGLGERDFYIDVKSDTSITLDEVKKAINENVLANVSIENNQIVYKGNKVSII
EDKVSISTNLNPKYFEILNISTHHPNPNEQYVRIRGVAFETEEQLKDYLSWLEKAEETDHRLIGEKLDLFSFHEEAGSGL
VLFHPKGQTIRNELIAFMREINDSMGYQEVYTSHVFKTDIWKISGHYTLYRDKLIVFNMEGDEYGVKPMNCPAHILIYKS
KPRTYRDLPIRFSEFGHVYRWEKKGELYGLLRVRGFVQDDGHIFLREDQLREEIKMLISKTVEVWHKFGFKDDDIKPYLS
TRPDESIGSDELWEKATNALISALQESGLKFGIKEKEGAFYGPKIDFEIRDSLGRWWQLSTIQVDFNLPERFKLEYIDKD
GIKKRPVMVHRAIYGSIDRFVAILLEHFKGKLPTWLSSVQVRVLPITDEVNEYAEKVLNDMRKRRIRAEIDYAGETLSKR
IKNAYDQGVPYILIVGKKEASEGTVTVRARGNIEVRNVKFEKFLELLITEIAQRDVEQTTVKALK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=406, Percent_Identity=43.8423645320197, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI38570111, Length=410, Percent_Identity=41.219512195122, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI20070344, Length=422, Percent_Identity=39.5734597156398, Blast_Score=320, Evalue=3e-87,
Organism=Escherichia coli, GI1788013, Length=431, Percent_Identity=41.7633410672854, Blast_Score=356, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI71984192, Length=422, Percent_Identity=41.7061611374408, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI71984184, Length=422, Percent_Identity=41.7061611374408, Blast_Score=335, Evalue=3e-92,
Organism=Saccharomyces cerevisiae, GI6322112, Length=410, Percent_Identity=41.4634146341463, Blast_Score=330, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6322654, Length=418, Percent_Identity=35.6459330143541, Blast_Score=256, Evalue=9e-69,
Organism=Drosophila melanogaster, GI24583839, Length=405, Percent_Identity=42.4691358024691, Blast_Score=334, Evalue=8e-92,
Organism=Drosophila melanogaster, GI24583842, Length=405, Percent_Identity=42.4691358024691, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI28574141, Length=405, Percent_Identity=42.4691358024691, Blast_Score=334, Evalue=9e-92,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 63231; Mature: 63231

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESYKPVWLKGAVILAINLIDKGYKPVAVGLGERDFYIDVKSDTSITLDEVKKAINENVL
CCCCCCEEECCEEEEEEEECCCCCCEEEEECCCCEEEEEECCCCCEEHHHHHHHHCCCEE
ANVSIENNQIVYKGNKVSIIEDKVSISTNLNPKYFEILNISTHHPNPNEQYVRIRGVAFE
EEEEECCCEEEEECCEEEEEECCEEEECCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEC
TEEQLKDYLSWLEKAEETDHRLIGEKLDLFSFHEEAGSGLVLFHPKGQTIRNELIAFMRE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCEEEECCCCCHHHHHHHHHHHH
INDSMGYQEVYTSHVFKTDIWKISGHYTLYRDKLIVFNMEGDEYGVKPMNCPAHILIYKS
HHHCCCHHHHHHHHHHEEEEEEECCCEEEEECEEEEEEECCCCCCCCCCCCCEEEEEECC
KPRTYRDLPIRFSEFGHVYRWEKKGELYGLLRVRGFVQDDGHIFLREDQLREEIKMLISK
CCCCCCCCCCCHHCCCCEEEECCCCCEEEEEEEEEEEECCCEEEEEHHHHHHHHHHHHHH
TVEVWHKFGFKDDDIKPYLSTRPDESIGSDELWEKATNALISALQESGLKFGIKEKEGAF
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCE
YGPKIDFEIRDSLGRWWQLSTIQVDFNLPERFKLEYIDKDGIKKRPVMVHRAIYGSIDRF
ECCEECEEECCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
VAILLEHFKGKLPTWLSSVQVRVLPITDEVNEYAEKVLNDMRKRRIRAEIDYAGETLSKR
HHHHHHHHCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
IKNAYDQGVPYILIVGKKEASEGTVTVRARGNIEVRNVKFEKFLELLITEIAQRDVEQTT
HHHHHHCCCCEEEEEECCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
VKALK
HHHCC
>Mature Secondary Structure
MESYKPVWLKGAVILAINLIDKGYKPVAVGLGERDFYIDVKSDTSITLDEVKKAINENVL
CCCCCCEEECCEEEEEEEECCCCCCEEEEECCCCEEEEEECCCCCEEHHHHHHHHCCCEE
ANVSIENNQIVYKGNKVSIIEDKVSISTNLNPKYFEILNISTHHPNPNEQYVRIRGVAFE
EEEEECCCEEEEECCEEEEEECCEEEECCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEC
TEEQLKDYLSWLEKAEETDHRLIGEKLDLFSFHEEAGSGLVLFHPKGQTIRNELIAFMRE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCEEEECCCCCHHHHHHHHHHHH
INDSMGYQEVYTSHVFKTDIWKISGHYTLYRDKLIVFNMEGDEYGVKPMNCPAHILIYKS
HHHCCCHHHHHHHHHHEEEEEEECCCEEEEECEEEEEEECCCCCCCCCCCCCEEEEEECC
KPRTYRDLPIRFSEFGHVYRWEKKGELYGLLRVRGFVQDDGHIFLREDQLREEIKMLISK
CCCCCCCCCCCHHCCCCEEEECCCCCEEEEEEEEEEEECCCEEEEEHHHHHHHHHHHHHH
TVEVWHKFGFKDDDIKPYLSTRPDESIGSDELWEKATNALISALQESGLKFGIKEKEGAF
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCE
YGPKIDFEIRDSLGRWWQLSTIQVDFNLPERFKLEYIDKDGIKKRPVMVHRAIYGSIDRF
ECCEECEEECCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
VAILLEHFKGKLPTWLSSVQVRVLPITDEVNEYAEKVLNDMRKRRIRAEIDYAGETLSKR
HHHHHHHHCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
IKNAYDQGVPYILIVGKKEASEGTVTVRARGNIEVRNVKFEKFLELLITEIAQRDVEQTT
HHHHHHCCCCEEEEEECCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
VKALK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA