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The map label for this gene is phrB [H]

Identifier: 15899214

GI number: 15899214

Start: 2242506

End: 2243807

Strand: Reverse

Name: phrB [H]

Synonym: SSO2472

Alternate gene names: 15899214

Gene position: 2243807-2242506 (Counterclockwise)

Preceding gene: 15899217

Following gene: 15899213

Centisome position: 74.99

GC content: 33.95

Gene sequence:

>1302_bases
GTGCTCTGCCTATTTATATTTAGAAGAGATCTGAGACTAGACGACAATACTGGATTAATTAAAGCTTTAGAGGATTGCGA
GAAAGTTATTCCAGCGTTTATATTAGATCCTAGACAAGTCGGTAATGAAAACGAATACAAATCAGAGTTTGCCATAAACT
TCATGATTAATTCATTGAATGAGCTAAATGACGAGTTAAGAAAAAGAGGATCCAGACTTTATGTCTACTTCGGACTAGCT
GAGGAGGTAATAAAGAACTTACTTAAAGATGTTGATGCAGTTTATCTAAATGAGGATTATACTCCATTTAGTAAAATGAG
GGACGAAAGAATTCGCAAGTACTGTGAAGATACCGGAAAAATTATGAAATCTTTTGAGGATTACTTACTCACATCAAAGA
ACGATTTTAAAAACTATAGGAACTTCACAACATTTTATAATGTAGTAAAAAATAAACAAATAAAGAAACCAGTTCAAAAT
AATTATACAAATTACTATAAAAATTCTTTAGGTGACGAGCATGAATTACCACCTAGACAAGGAGAACGAGGAGGAAGAAA
AGAGGGCATTAAATTAATAGAGAGAGCAAGGCAAATTAATTACGATAGGAAAGATTTCGTAGCAGAGGATAATAGAACTT
TTCTTTCACCCCATCTGAAATTTGGAACCTTGTCAATTAGAGAGGTTTATTATTCTCTATTAGACAGTCAAGCTATAATT
AGGCAGTTATACTGGAGGGATTTCTATACTTTATTGGCTTACTATAATGAGAGAGTATTTCATGAACCGTTAAAAAGGGA
ATATAATTGCATTGAGTGGGAGAACAATGAAAGGCTATTTCAAGCTTGGCTTGAGGGAAAAACTGGATATCCAATAATAG
ATGCTGGAATGAGACAATTGAACCGGACTGGTGATATGCCGAACAGAGTTAGAATGTTAACCGCATTTTTCCTAGTTAAG
GTTCTTATAATTGATTGGAGGATAGGTGAGAAATACTTTGCAAGTAAATTAATTGATTATGATCCTTCGGTTAACAATGG
AAACTGGCAATGGATAGCCTCTGTAGGTACTGATTATATATTTAGAGTATTTGACCCTTGGAAACAACAGGTTACATATG
ATCCGGAGGCAAAGTATATAAAAAGGTGGGTAGATGAATTGGAAAGCTATGACGCAGAGATCATACATAATGCATACAAA
TATACTCTGAAGAGCTACCCTAAACCTATAGTAGATTGGAGAATAAGGGTAAATCTCGCAAAGAGACTTTACGAGGTATG
TAGGAAATCTAAAATAAAATAG

Upstream 100 bases:

>100_bases
AAGATCAAATATCTAGTCATATAATATATAATGTTAAAAGCTTCCTTTTATTCTAACTCTTTTTAATATAAATCTAATAA
CTCGAGAACTGAAAAGAAAT

Downstream 100 bases:

>100_bases
GTTTCTTTTTCGGATTTAGTACGATGTAAATGTAATTTCTTCGTAAGAAATGCTTATTTATTAATAATTTTAACTCCTTA
TTGATGTCGGCAAAAACGTT

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLCLFIFRRDLRLDDNTGLIKALEDCEKVIPAFILDPRQVGNENEYKSEFAINFMINSLNELNDELRKRGSRLYVYFGLA
EEVIKNLLKDVDAVYLNEDYTPFSKMRDERIRKYCEDTGKIMKSFEDYLLTSKNDFKNYRNFTTFYNVVKNKQIKKPVQN
NYTNYYKNSLGDEHELPPRQGERGGRKEGIKLIERARQINYDRKDFVAEDNRTFLSPHLKFGTLSIREVYYSLLDSQAII
RQLYWRDFYTLLAYYNERVFHEPLKREYNCIEWENNERLFQAWLEGKTGYPIIDAGMRQLNRTGDMPNRVRMLTAFFLVK
VLIIDWRIGEKYFASKLIDYDPSVNNGNWQWIASVGTDYIFRVFDPWKQQVTYDPEAKYIKRWVDELESYDAEIIHNAYK
YTLKSYPKPIVDWRIRVNLAKRLYEVCRKSKIK

Sequences:

>Translated_433_residues
MLCLFIFRRDLRLDDNTGLIKALEDCEKVIPAFILDPRQVGNENEYKSEFAINFMINSLNELNDELRKRGSRLYVYFGLA
EEVIKNLLKDVDAVYLNEDYTPFSKMRDERIRKYCEDTGKIMKSFEDYLLTSKNDFKNYRNFTTFYNVVKNKQIKKPVQN
NYTNYYKNSLGDEHELPPRQGERGGRKEGIKLIERARQINYDRKDFVAEDNRTFLSPHLKFGTLSIREVYYSLLDSQAII
RQLYWRDFYTLLAYYNERVFHEPLKREYNCIEWENNERLFQAWLEGKTGYPIIDAGMRQLNRTGDMPNRVRMLTAFFLVK
VLIIDWRIGEKYFASKLIDYDPSVNNGNWQWIASVGTDYIFRVFDPWKQQVTYDPEAKYIKRWVDELESYDAEIIHNAYK
YTLKSYPKPIVDWRIRVNLAKRLYEVCRKSKIK
>Mature_433_residues
MLCLFIFRRDLRLDDNTGLIKALEDCEKVIPAFILDPRQVGNENEYKSEFAINFMINSLNELNDELRKRGSRLYVYFGLA
EEVIKNLLKDVDAVYLNEDYTPFSKMRDERIRKYCEDTGKIMKSFEDYLLTSKNDFKNYRNFTTFYNVVKNKQIKKPVQN
NYTNYYKNSLGDEHELPPRQGERGGRKEGIKLIERARQINYDRKDFVAEDNRTFLSPHLKFGTLSIREVYYSLLDSQAII
RQLYWRDFYTLLAYYNERVFHEPLKREYNCIEWENNERLFQAWLEGKTGYPIIDAGMRQLNRTGDMPNRVRMLTAFFLVK
VLIIDWRIGEKYFASKLIDYDPSVNNGNWQWIASVGTDYIFRVFDPWKQQVTYDPEAKYIKRWVDELESYDAEIIHNAYK
YTLKSYPKPIVDWRIRVNLAKRLYEVCRKSKIK

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=488, Percent_Identity=29.7131147540984, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4758072, Length=490, Percent_Identity=27.9591836734694, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI188536103, Length=439, Percent_Identity=29.1571753986333, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1786926, Length=476, Percent_Identity=31.9327731092437, Blast_Score=218, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6324962, Length=194, Percent_Identity=40.2061855670103, Blast_Score=133, Evalue=7e-32,
Organism=Drosophila melanogaster, GI17137248, Length=460, Percent_Identity=29.7826086956522, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24585455, Length=460, Percent_Identity=29.7826086956522, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24648152, Length=497, Percent_Identity=25.7545271629779, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 52018; Mature: 52018

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCLFIFRRDLRLDDNTGLIKALEDCEKVIPAFILDPRQVGNENEYKSEFAINFMINSLN
CEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHH
ELNDELRKRGSRLYVYFGLAEEVIKNLLKDVDAVYLNEDYTPFSKMRDERIRKYCEDTGK
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHH
IMKSFEDYLLTSKNDFKNYRNFTTFYNVVKNKQIKKPVQNNYTNYYKNSLGDEHELPPRQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHCCCCCCCCCCCC
GERGGRKEGIKLIERARQINYDRKDFVAEDNRTFLSPHLKFGTLSIREVYYSLLDSQAII
CCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
RQLYWRDFYTLLAYYNERVFHEPLKREYNCIEWENNERLFQAWLEGKTGYPIIDAGMRQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHH
NRTGDMPNRVRMLTAFFLVKVLIIDWRIGEKYFASKLIDYDPSVNNGNWQWIASVGTDYI
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEECCCHHH
FRVFDPWKQQVTYDPEAKYIKRWVDELESYDAEIIHNAYKYTLKSYPKPIVDWRIRVNLA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KRLYEVCRKSKIK
HHHHHHHHHCCCC
>Mature Secondary Structure
MLCLFIFRRDLRLDDNTGLIKALEDCEKVIPAFILDPRQVGNENEYKSEFAINFMINSLN
CEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHH
ELNDELRKRGSRLYVYFGLAEEVIKNLLKDVDAVYLNEDYTPFSKMRDERIRKYCEDTGK
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHH
IMKSFEDYLLTSKNDFKNYRNFTTFYNVVKNKQIKKPVQNNYTNYYKNSLGDEHELPPRQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHCCCCCCCCCCCC
GERGGRKEGIKLIERARQINYDRKDFVAEDNRTFLSPHLKFGTLSIREVYYSLLDSQAII
CCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
RQLYWRDFYTLLAYYNERVFHEPLKREYNCIEWENNERLFQAWLEGKTGYPIIDAGMRQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHH
NRTGDMPNRVRMLTAFFLVKVLIIDWRIGEKYFASKLIDYDPSVNNGNWQWIASVGTDYI
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEECCCHHH
FRVFDPWKQQVTYDPEAKYIKRWVDELESYDAEIIHNAYKYTLKSYPKPIVDWRIRVNLA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KRLYEVCRKSKIK
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2681164; 11016950 [H]