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The map label for this gene is rnhB

Identifier: 15899137

GI number: 15899137

Start: 2170481

End: 2171119

Strand: Direct

Name: rnhB

Synonym: SSO2384

Alternate gene names: 15899137

Gene position: 2170481-2171119 (Clockwise)

Preceding gene: 15899136

Following gene: 15899138

Centisome position: 72.54

GC content: 36.31

Gene sequence:

>639_bases
ATGAGAGTAGGCATAGATGAAGCAGGTCGTGGAGCGTTAATAGGTCCAATGATAGTTGCAGGTGTAGTTATAAGTGATAC
GAAACTAAAGTTCCTTAAAGGTATTGGAGTAAAGGATAGTAAACAATTAACGAGAGAAAGAAGGGAGAAATTGTTCGATA
TCGTTGCGAATACCGTAGAGGCTTTCACAGTAGTTAAAGTCTTTCCTTACGAAATAGATAATTATAACTTGAACGATCTA
ACATATGACGCAGTATCTAAGATAATACTATCACTTTCATCTTTCAATCCTGAGATAGTAACAGTTGATAAAGTGGGAGA
TGAGAAGCCCGTTATAGAACTTATCAATAAGCTGGGTTATAAGTCAAATGTAGTTCACAAGGCGGATGTCCTATTTGTTG
AAGCAAGTGCAGCAAGTATTATAGCTAAAGTAATAAGGGATAACTATATTGATGAGTTGAAACAAGTTTACGGTGATTTT
GGGAGTGGATACCCTGCTGACCCTAGAACAATAAAGTGGCTTAAGTCCTTTTATGAGAAGAACCCAAATCCACCCCCTAT
TATCAGAAGATCATGGAAAATTTTACGTTCTACTGCTCCATTATATTATATAAGTAAAGAGGGTAGAAGGTTATGGTAA

Upstream 100 bases:

>100_bases
TGATATCTTCTATTGTGGACGTTGAAGTTAATGAGAGTTATCAGAATGAAAAGATGAAAAACGGTAAAAGGGAGGCAAAA
CTAGTTATTTCATTGAGGAT

Downstream 100 bases:

>100_bases
CGAATTTACCTGCCGAAGCTAAGGCTAAGTGGCTAAAGGTAATGGACGCAAAGACTCCAGAGGAGAAGTTCCAAGCCCTA
CAAGAATTTCTTAAGGAAGT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRVGIDEAGRGALIGPMIVAGVVISDTKLKFLKGIGVKDSKQLTRERREKLFDIVANTVEAFTVVKVFPYEIDNYNLNDL
TYDAVSKIILSLSSFNPEIVTVDKVGDEKPVIELINKLGYKSNVVHKADVLFVEASAASIIAKVIRDNYIDELKQVYGDF
GSGYPADPRTIKWLKSFYEKNPNPPPIIRRSWKILRSTAPLYYISKEGRRLW

Sequences:

>Translated_212_residues
MRVGIDEAGRGALIGPMIVAGVVISDTKLKFLKGIGVKDSKQLTRERREKLFDIVANTVEAFTVVKVFPYEIDNYNLNDL
TYDAVSKIILSLSSFNPEIVTVDKVGDEKPVIELINKLGYKSNVVHKADVLFVEASAASIIAKVIRDNYIDELKQVYGDF
GSGYPADPRTIKWLKSFYEKNPNPPPIIRRSWKILRSTAPLYYISKEGRRLW
>Mature_212_residues
MRVGIDEAGRGALIGPMIVAGVVISDTKLKFLKGIGVKDSKQLTRERREKLFDIVANTVEAFTVVKVFPYEIDNYNLNDL
TYDAVSKIILSLSSFNPEIVTVDKVGDEKPVIELINKLGYKSNVVHKADVLFVEASAASIIAKVIRDNYIDELKQVYGDF
GSGYPADPRTIKWLKSFYEKNPNPPPIIRRSWKILRSTAPLYYISKEGRRLW

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Homo sapiens, GI38455391, Length=213, Percent_Identity=34.2723004694836, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1786380, Length=167, Percent_Identity=32.3353293413174, Blast_Score=76, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536263, Length=214, Percent_Identity=33.1775700934579, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324257, Length=226, Percent_Identity=33.6283185840708, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24580635, Length=213, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_SULSO (Q97W56)

Other databases:

- EMBL:   AE006641
- PIR:   E90409
- RefSeq:   NP_343742.1
- ProteinModelPortal:   Q97W56
- SMR:   Q97W56
- GeneID:   1453859
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO2384
- NMPDR:   fig|273057.1.peg.2165
- HOGENOM:   HBG584843
- OMA:   AEHKADE
- PhylomeDB:   Q97W56
- ProtClustDB:   PRK00015
- BioCyc:   SSOL273057:SSO2384-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_A
- InterPro:   IPR004649
- InterPro:   IPR001352
- InterPro:   IPR020787
- InterPro:   IPR023160
- InterPro:   IPR012337
- Gene3D:   G3DSA:1.10.10.460
- PANTHER:   PTHR10954
- TIGRFAMs:   TIGR00729

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23925; Mature: 23925

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGIDEAGRGALIGPMIVAGVVISDTKLKFLKGIGVKDSKQLTRERREKLFDIVANTVE
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFTVVKVFPYEIDNYNLNDLTYDAVSKIILSLSSFNPEIVTVDKVGDEKPVIELINKLGY
HHHHEEEECEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCC
KSNVVHKADVLFVEASAASIIAKVIRDNYIDELKQVYGDFGSGYPADPRTIKWLKSFYEK
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NPNPPPIIRRSWKILRSTAPLYYISKEGRRLW
CCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure
MRVGIDEAGRGALIGPMIVAGVVISDTKLKFLKGIGVKDSKQLTRERREKLFDIVANTVE
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFTVVKVFPYEIDNYNLNDLTYDAVSKIILSLSSFNPEIVTVDKVGDEKPVIELINKLGY
HHHHEEEECEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCC
KSNVVHKADVLFVEASAASIIAKVIRDNYIDELKQVYGDFGSGYPADPRTIKWLKSFYEK
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NPNPPPIIRRSWKILRSTAPLYYISKEGRRLW
CCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726