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The map label for this gene is glcA [H]

Identifier: 15898910

GI number: 15898910

Start: 1951498

End: 1953090

Strand: Direct

Name: glcA [H]

Synonym: SSO2126

Alternate gene names: 15898910

Gene position: 1951498-1953090 (Clockwise)

Preceding gene: 15898909

Following gene: 15898911

Centisome position: 65.22

GC content: 38.23

Gene sequence:

>1593_bases
ATGTATATACAACCCTTAAACCCAACTGGTAATGTAGGGTTAACTATTTTAGCATCCTTAACACCAATAATTGCACTATT
AATATTGTTGGCTGGGCTAAGAATAACTGCATGGCTAGCTACGCTCATAGGTTCGATAATAACGATTTTAGTTGCAACAT
TTGTATGGGGAACTCCAATAATACAAGTTAGTTATGCATGGCTTATAGGAGCATTAGTAGGAACTTGGGCAATATCTTGG
ATAGTATTCTGGGGGTTAACAATTTATAATACGTTAGTGTTAACTGGGAAATTTGACGCATTTAGAGACTGGATAGTGAG
AAATTCTACTAATGACGCTAGAGTTCAAGCTATAGTATTAGCGTGGTCTTTAGGAGCCTTATTTGAAGGACTGGTGGGAT
TTGGATATCCGTGGGCGTTTATCGCCCCACTCCTAATATATTTAGGTTTTGATGATCTAAAAGCTCTTCAAGTGTCCGCA
TTAGCTAATAACGCCCCAGTATCTTTTGGTGCATTAGGAACTCCAATAATTATTCTAGCGTCAGTTACTGGATTACCCTT
ATTATTCATATCATCTTCAGTTGCGAAAGTAGTGGCAATTTTGGCTCTATTACCACCTTGGATATTATTATATCTAGTTG
ATAAATGGAGGGGGATAAGAGAAGCATGGCCAATAGCTATACTTGGTTCACTATCCTATATTTTAGGTCAATATCCAGTA
GCGAGTTTTATTGGTCCTTATTTACCGGACATTACAGGATCTTTAATATCTTTCGTCATATTACTTTTATTCTTAAGAGT
GTGGAGACCTAAGAGAGTGCTGAGTCTAAGAAGCGTTCAGCCTAATGGTGGGGAGAGCAAGAAATACTCAACAAAAGACG
TTATTGAGGCCTTATCAGCCTTCATAGTGTTAATTATAGTAGTAACTCTATGGACTGGTCCTTGGTCACCACTAACTAAG
TTTTCATTAGTAACCTTATCTCAAACAGCATACTCCTCTCTTCTCCATAAAAATGTAGGAGTAAGTTTTGCATTCAATCC
TTTCGTAGCGGGTACGTCAATTTTAGTATCATGGATAATTATTTCTCTAGTTTTAAGGGCTTCGCCAAGAATTATGGGAG
AAGCTATAAAGAGGTCTTTTCATCAATACTGGGGAGGGATATTAACTGGTATTTTTGTGGTTGGGCTTGCATATGTATTT
AATTTCAGCGGAATGGCTTACTCATTAGCTTGGAAAACTAGTGACCTAGGTATAGCATTTATAATAGTTTCACCCTTATT
TGGTTGGATTGGATGCGCATTATCAGGGAGTAATACGTCGACCAACGCCCTTTTTGGGGCGTTTCAAGTTACAACTGCAC
GTTTAACTGGTTTGCCCGTAGGATTACCACCAGCGTTAAATTCTGTGGGTGCTGAGTTAGCTAAACCAGTTGCTCCACAG
ACTGCTAGTGCAGGAGTATCCACAACAAAATACGTTCGAAAAGAGGGTATAGTGATTAGGAAAAATCTACCTTGGGCTAT
AGGCATTTTGATATATCTAATACTTATAGGAGTACTATACGCATTCTTAGCTCCATCTCTATTTGTACATTAG

Upstream 100 bases:

>100_bases
ATGACGAGATGTAAGAAATTTTGTAATTATTTTTAAGAATTTTCAATCATTTATCAAAAGTAAGCTTATAAAGTTAGAGA
TATATTCCAATTAGAGAAGT

Downstream 100 bases:

>100_bases
TGTTAGATATATGAATTAACTTCCTTTTTTCTATTAAGTTAACTCCTAATGTTTTTACATCTCTAGGATTGTTTAACTTA
AATTATTCTTCTCAAAGTTT

Product: L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MYIQPLNPTGNVGLTILASLTPIIALLILLAGLRITAWLATLIGSIITILVATFVWGTPIIQVSYAWLIGALVGTWAISW
IVFWGLTIYNTLVLTGKFDAFRDWIVRNSTNDARVQAIVLAWSLGALFEGLVGFGYPWAFIAPLLIYLGFDDLKALQVSA
LANNAPVSFGALGTPIIILASVTGLPLLFISSSVAKVVAILALLPPWILLYLVDKWRGIREAWPIAILGSLSYILGQYPV
ASFIGPYLPDITGSLISFVILLLFLRVWRPKRVLSLRSVQPNGGESKKYSTKDVIEALSAFIVLIIVVTLWTGPWSPLTK
FSLVTLSQTAYSSLLHKNVGVSFAFNPFVAGTSILVSWIIISLVLRASPRIMGEAIKRSFHQYWGGILTGIFVVGLAYVF
NFSGMAYSLAWKTSDLGIAFIIVSPLFGWIGCALSGSNTSTNALFGAFQVTTARLTGLPVGLPPALNSVGAELAKPVAPQ
TASAGVSTTKYVRKEGIVIRKNLPWAIGILIYLILIGVLYAFLAPSLFVH

Sequences:

>Translated_530_residues
MYIQPLNPTGNVGLTILASLTPIIALLILLAGLRITAWLATLIGSIITILVATFVWGTPIIQVSYAWLIGALVGTWAISW
IVFWGLTIYNTLVLTGKFDAFRDWIVRNSTNDARVQAIVLAWSLGALFEGLVGFGYPWAFIAPLLIYLGFDDLKALQVSA
LANNAPVSFGALGTPIIILASVTGLPLLFISSSVAKVVAILALLPPWILLYLVDKWRGIREAWPIAILGSLSYILGQYPV
ASFIGPYLPDITGSLISFVILLLFLRVWRPKRVLSLRSVQPNGGESKKYSTKDVIEALSAFIVLIIVVTLWTGPWSPLTK
FSLVTLSQTAYSSLLHKNVGVSFAFNPFVAGTSILVSWIIISLVLRASPRIMGEAIKRSFHQYWGGILTGIFVVGLAYVF
NFSGMAYSLAWKTSDLGIAFIIVSPLFGWIGCALSGSNTSTNALFGAFQVTTARLTGLPVGLPPALNSVGAELAKPVAPQ
TASAGVSTTKYVRKEGIVIRKNLPWAIGILIYLILIGVLYAFLAPSLFVH
>Mature_530_residues
MYIQPLNPTGNVGLTILASLTPIIALLILLAGLRITAWLATLIGSIITILVATFVWGTPIIQVSYAWLIGALVGTWAISW
IVFWGLTIYNTLVLTGKFDAFRDWIVRNSTNDARVQAIVLAWSLGALFEGLVGFGYPWAFIAPLLIYLGFDDLKALQVSA
LANNAPVSFGALGTPIIILASVTGLPLLFISSSVAKVVAILALLPPWILLYLVDKWRGIREAWPIAILGSLSYILGQYPV
ASFIGPYLPDITGSLISFVILLLFLRVWRPKRVLSLRSVQPNGGESKKYSTKDVIEALSAFIVLIIVVTLWTGPWSPLTK
FSLVTLSQTAYSSLLHKNVGVSFAFNPFVAGTSILVSWIIISLVLRASPRIMGEAIKRSFHQYWGGILTGIFVVGLAYVF
NFSGMAYSLAWKTSDLGIAFIIVSPLFGWIGCALSGSNTSTNALFGAFQVTTARLTGLPVGLPPALNSVGAELAKPVAPQ
TASAGVSTTKYVRKEGIVIRKNLPWAIGILIYLILIGVLYAFLAPSLFVH

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=555, Percent_Identity=34.0540540540541, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790031, Length=545, Percent_Identity=30.2752293577982, Blast_Score=214, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 57226; Mature: 57226

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIQPLNPTGNVGLTILASLTPIIALLILLAGLRITAWLATLIGSIITILVATFVWGTPI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
IQVSYAWLIGALVGTWAISWIVFWGLTIYNTLVLTGKFDAFRDWIVRNSTNDARVQAIVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCCCCHHHHHHHH
AWSLGALFEGLVGFGYPWAFIAPLLIYLGFDDLKALQVSALANNAPVSFGALGTPIIILA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
SVTGLPLLFISSSVAKVVAILALLPPWILLYLVDKWRGIREAWPIAILGSLSYILGQYPV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCH
ASFIGPYLPDITGSLISFVILLLFLRVWRPKRVLSLRSVQPNGGESKKYSTKDVIEALSA
HHHHCHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
FIVLIIVVTLWTGPWSPLTKFSLVTLSQTAYSSLLHKNVGVSFAFNPFVAGTSILVSWII
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHH
ISLVLRASPRIMGEAIKRSFHQYWGGILTGIFVVGLAYVFNFSGMAYSLAWKTSDLGIAF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHH
IIVSPLFGWIGCALSGSNTSTNALFGAFQVTTARLTGLPVGLPPALNSVGAELAKPVAPQ
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
TASAGVSTTKYVRKEGIVIRKNLPWAIGILIYLILIGVLYAFLAPSLFVH
CCCCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYIQPLNPTGNVGLTILASLTPIIALLILLAGLRITAWLATLIGSIITILVATFVWGTPI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
IQVSYAWLIGALVGTWAISWIVFWGLTIYNTLVLTGKFDAFRDWIVRNSTNDARVQAIVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCCCCHHHHHHHH
AWSLGALFEGLVGFGYPWAFIAPLLIYLGFDDLKALQVSALANNAPVSFGALGTPIIILA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
SVTGLPLLFISSSVAKVVAILALLPPWILLYLVDKWRGIREAWPIAILGSLSYILGQYPV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCH
ASFIGPYLPDITGSLISFVILLLFLRVWRPKRVLSLRSVQPNGGESKKYSTKDVIEALSA
HHHHCHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
FIVLIIVVTLWTGPWSPLTKFSLVTLSQTAYSSLLHKNVGVSFAFNPFVAGTSILVSWII
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHH
ISLVLRASPRIMGEAIKRSFHQYWGGILTGIFVVGLAYVFNFSGMAYSLAWKTSDLGIAF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHH
IIVSPLFGWIGCALSGSNTSTNALFGAFQVTTARLTGLPVGLPPALNSVGAELAKPVAPQ
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
TASAGVSTTKYVRKEGIVIRKNLPWAIGILIYLILIGVLYAFLAPSLFVH
CCCCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]