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The map label for this gene is acsA-4 [H]

Identifier: 15898811

GI number: 15898811

Start: 1834088

End: 1834954

Strand: Direct

Name: acsA-4 [H]

Synonym: SSO2020

Alternate gene names: 15898811

Gene position: 1834088-1834954 (Clockwise)

Preceding gene: 15898810

Following gene: 15898812

Centisome position: 61.29

GC content: 37.83

Gene sequence:

>867_bases
ATGACTGCTAAGTATGGTAGAATTTTAAATGTAACTGATGCGTTGTTTTCAAAATGGGAAGATAGTGAGGCAGATAAAAG
AATAGCAATTTATTATAAAGATGAAGTTTGGTCATATCGCAAGGTAATAGAGGAAATAAATAGGGTAGGTAATGCCCTAA
GGGAATTAGGAGTGGAGAAAGAAAATAGAATATTGATAATAGCGTATGATACACCGTTCTTTATATCGATTTTCTATAGT
GCCATGAAAATTGGTGCGATCCCGGTACCAGTTAACACTTATTTAAAACCAGAGGAGTATCTTTTCCTTTTGGAGGATAC
TGGAGCTAAGGTATTGGCAATTGAACCAGACATTTGGCCCAAGTTATCTCCATACCTTAAAGGTAGAGCTGATAAACTTA
AACATGTTATAATTTTGCCCAGTATATCAGAAACGCAGAAGATCACTTTCGAGGATCATAATCACAAGGCAGAGTTGAGT
TTTTATCATGGACTTGTTTCTACAGCCTCCACTCACTTAGAAGCTGAAAAGACTTCTCCAAACGATATGGCGTTTTGGCT
CTATACGTCAGGGACTACGGGACATCCTAAAGCAGCAGTTCACTTACATAAAGACATATTAGTTGTTCTCGATACCTATG
TGAAAAACGTTCTCAAAATCAGTGAAAAAGATAGACTATTCAGCGCCTCTAAACTATTTTTCGCTTATGGATTAGGAAAT
GGATCCTACTTCGCCTTTGGTAACGGTGCCTCGACAGTTCTGATGCCAGATAGAGTAGAACCAGAAGGGTATTGGAGACC
ATACATAAATACAAGCCTACTATCTTCTTTGGAGTGCCTACTTTATATAACGCAATGCTACATGTAG

Upstream 100 bases:

>100_bases
AAATCTCCACTATAATATTGTACTAAAATAAATTGCGAGATTTTAACGTACTAGATTAACAGCGTAAACTATATAAATTG
GTTTAAAAAATTAAATAATA

Downstream 100 bases:

>100_bases
AGGATTGGAAGAATTACAACTTAACTAGTTTAAGATTTTGCGTTTCTGCTGGAGAACCCTTACCCCCGTTAATATATATT
AAATGGAAAGAGAGATACGG

Product: Acetyl-CoA synthetase (acetate-CoA ligase) amino-end fragment (acsA-4)

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTAKYGRILNVTDALFSKWEDSEADKRIAIYYKDEVWSYRKVIEEINRVGNALRELGVEKENRILIIAYDTPFFISIFYS
AMKIGAIPVPVNTYLKPEEYLFLLEDTGAKVLAIEPDIWPKLSPYLKGRADKLKHVIILPSISETQKITFEDHNHKAELS
FYHGLVSTASTHLEAEKTSPNDMAFWLYTSGTTGHPKAAVHLHKDILVVLDTYVKNVLKISEKDRLFSASKLFFAYGLGN
GSYFAFGNGASTVLMPDRVEPEGYWRPYINTSLLSSLECLLYITQCYM

Sequences:

>Translated_288_residues
MTAKYGRILNVTDALFSKWEDSEADKRIAIYYKDEVWSYRKVIEEINRVGNALRELGVEKENRILIIAYDTPFFISIFYS
AMKIGAIPVPVNTYLKPEEYLFLLEDTGAKVLAIEPDIWPKLSPYLKGRADKLKHVIILPSISETQKITFEDHNHKAELS
FYHGLVSTASTHLEAEKTSPNDMAFWLYTSGTTGHPKAAVHLHKDILVVLDTYVKNVLKISEKDRLFSASKLFFAYGLGN
GSYFAFGNGASTVLMPDRVEPEGYWRPYINTSLLSSLECLLYITQCYM
>Mature_287_residues
TAKYGRILNVTDALFSKWEDSEADKRIAIYYKDEVWSYRKVIEEINRVGNALRELGVEKENRILIIAYDTPFFISIFYSA
MKIGAIPVPVNTYLKPEEYLFLLEDTGAKVLAIEPDIWPKLSPYLKGRADKLKHVIILPSISETQKITFEDHNHKAELSF
YHGLVSTASTHLEAEKTSPNDMAFWLYTSGTTGHPKAAVHLHKDILVVLDTYVKNVLKISEKDRLFSASKLFFAYGLGNG
SYFAFGNGASTVLMPDRVEPEGYWRPYINTSLLSSLECLLYITQCYM

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Escherichia coli, GI221142682, Length=174, Percent_Identity=28.735632183908, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 32816; Mature: 32685

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKYGRILNVTDALFSKWEDSEADKRIAIYYKDEVWSYRKVIEEINRVGNALRELGVEK
CCCCCCCEEEHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
ENRILIIAYDTPFFISIFYSAMKIGAIPVPVNTYLKPEEYLFLLEDTGAKVLAIEPDIWP
CCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCC
KLSPYLKGRADKLKHVIILPSISETQKITFEDHNHKAELSFYHGLVSTASTHLEAEKTSP
CCCHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHEEECCCCC
NDMAFWLYTSGTTGHPKAAVHLHKDILVVLDTYVKNVLKISEKDRLFSASKLFFAYGLGN
CCEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCEEEEEEECCC
GSYFAFGNGASTVLMPDRVEPEGYWRPYINTSLLSSLECLLYITQCYM
CCEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAKYGRILNVTDALFSKWEDSEADKRIAIYYKDEVWSYRKVIEEINRVGNALRELGVEK
CCCCCCEEEHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
ENRILIIAYDTPFFISIFYSAMKIGAIPVPVNTYLKPEEYLFLLEDTGAKVLAIEPDIWP
CCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCC
KLSPYLKGRADKLKHVIILPSISETQKITFEDHNHKAELSFYHGLVSTASTHLEAEKTSP
CCCHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHEEECCCCC
NDMAFWLYTSGTTGHPKAAVHLHKDILVVLDTYVKNVLKISEKDRLFSASKLFFAYGLGN
CCEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCEEEEEEECCC
GSYFAFGNGASTVLMPDRVEPEGYWRPYINTSLLSSLECLLYITQCYM
CCEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]